Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,224,685 G→A 20.7% A334T (GCG→ACG)  pgi → glucosephosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,224,6850GA20.7% 437.9 / 101.6 187A334T (GCG→ACG) pgiglucosephosphate isomerase
Reads supporting (aligned to +/- strand):  ref base G (85/59);  new base A (21/17);  total (107/80)
Fisher's exact test for biased strand distribution p-value = 7.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 189 total aligned reads displayed.

GCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTA  >  CP009273/4224542‑4224832
                                                                                                                                               |                                                                                                                                                   
gCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTAAAACAATTTCTTTGGTGCGGAAACTGAAGCGAt                                                                                                                                                 <  1:2822236/148‑1 (MQ=255)
 cTTTGATAAGTTCGTTGAACTGCTTTCCGGCGCACACGCTATGGACAAGCATTTCTCCACCACGCCTGCCGTGAAAAACCTGCCTGTACTGCTGGCGCTGTTTGGCATCTGGTACAACAATTTATTTGGTGCGGAAACTGAAGCGAtt                                                                                                                                                <  2:2710400/148‑1 (MQ=255)
  tttGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTc                                                                                                                                               >  1:2670496/1‑148 (MQ=255)
  tttGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAACGATTc                                                                                                                                               >  2:1863583/1‑148 (MQ=255)
    ttaTAACTTCTTTGAACTGGTTTCGGGCGCACACGCGATTGACAAGCATTTCACCACCACGCCTGCCGAGAAAACCCTGCCTGTACTGCTGGCGGTGATTGGCTTCTGTTAAAAAAATTTCTTTGGTGCGGAAACTGAAACGATTCTg                                                                                                                                             <  2:585074/146‑1 (MQ=255)
     gATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGc                                                                                                                                            >  1:987299/1‑148 (MQ=255)
     gATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAACGATTCTGc                                                                                                                                            >  1:1325900/1‑148 (MQ=255)
        aaCTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGt                                                                                                                                         >  2:2832876/1‑148 (MQ=255)
         aCTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTa                                                                                                                                        >  2:228667/1‑148 (MQ=255)
         aCTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAACGATTCTGCCGTa                                                                                                                                        <  1:3288602/148‑1 (MQ=255)
                tGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAg                                                                                                                                 >  1:521708/1‑148 (MQ=255)
                 gAACTGCTTTCCGGCGCACACGCTATGGACACGCTTTTCGCCACCACGCCTGCCGACAAAAACCTGCCTGTACTGCTGGCGCTGTTTGGAATCTGTAACAACAATTTCTTGGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGt                                                                                                                                <  1:3079099/148‑1 (MQ=255)
                 gAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCCCGCCTGCCGAGAAAAACCTGCCTGTACGGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGt                                                                                                                                <  1:1562970/148‑1 (MQ=255)
                 gAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAATCGATTCTGCCGTATGACCAGt                                                                                                                                <  2:1476067/148‑1 (MQ=255)
                 gAACTGCTTTCCGGCGCACACGCGATGGACAAGAATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTAAAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGt                                                                                                                                <  1:3202840/148‑1 (MQ=255)
                 gAACTGCTGTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGt                                                                                                                                <  2:846785/148‑1 (MQ=255)
                  aaCTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGta                                                                                                                               >  1:1231918/1‑148 (MQ=255)
                   aCTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGtat                                                                                                                              <  2:1078506/148‑1 (MQ=255)
                    cTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGtata                                                                                                                             <  2:2567360/148‑1 (MQ=255)
                    cTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAACGATTCTGCCGTATGACCAGtata                                                                                                                             <  2:2732233/148‑1 (MQ=255)
                     tGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTatat                                                                                                                            >  1:3071493/1‑148 (MQ=255)
                      gCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATg                                                                                                                           <  1:542960/148‑1 (MQ=255)
                              gcgcACACGCGATGGACAAGCATTTCTCCACCCCGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTGGGTGCGGAAACTGAAACGATTCTGCCGTATGACCAGTATATGCACCGttt                                                                                                                   <  1:431463/148‑1 (MQ=255)
                              gcgcACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAATCGATTCTGCCGTATGACCAGTATATGCACCGttt                                                                                                                   <  1:3084902/148‑1 (MQ=255)
                              gcgcACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAACGATTCTGCCGTATGACCAGTATATGCACCGttt                                                                                                                   <  1:1634032/148‑1 (MQ=255)
                                 cacaCGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTcgc                                                                                                                >  2:2908915/1‑148 (MQ=255)
                                  acacGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTcgcg                                                                                                               >  1:883585/1‑148 (MQ=255)
                                   cacGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCgcgg                                                                                                              >  2:1749812/1‑148 (MQ=255)
                                     cgcgATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAACGATTCTGCCGTATGACCAGTATATGCACCGTTTCgcggcg                                                                                                            >  2:777647/1‑148 (MQ=255)
                                       cgATGGACACGCTTTTCTCCCCCCCGCCTGCCGAGAAAAACCTGCCTGTACTGCGGGCGCTGATTGGCATCGGGTACAACAATTTCTTTGGGGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTa                                                                                                          <  1:570099/148‑1 (MQ=255)
                                         aTGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACt                                                                                                        >  2:1879026/1‑148 (MQ=255)
                                           ggACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTc                                                                                                      >  2:2257715/1‑148 (MQ=255)
                                           ggACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTc                                                                                                      <  1:2739182/148‑1 (MQ=255)
                                            gACACGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTGGGTGCGGAAACTGAAACGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTcc                                                                                                     <  1:2730321/148‑1 (MQ=255)
                                                aGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCcagca                                                                                                 >  1:1054872/1‑148 (MQ=255)
                                                    tttCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAACGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGc                                                                                             >  2:991950/1‑148 (MQ=255)
                                                     ttCTCCACCCCGCCTGCCGAGAAAAACCGGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCa                                                                                            <  1:711537/148‑1 (MQ=255)
                                                     ttCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCa                                                                                            >  2:2358866/1‑148 (MQ=255)
                                                             cacGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAg                                                                                    >  1:2302761/1‑148 (MQ=255)
                                                              acGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGt                                                                                   >  2:2705639/1‑148 (MQ=255)
                                                              acGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGt                                                                                   >  2:2705906/1‑148 (MQ=255)
                                                              acGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGt                                                                                   >  2:1701905/1‑148 (MQ=255)
                                                              acGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGt                                                                                   >  2:3142794/1‑148 (MQ=255)
                                                                 ccTGCCTAGAAAAACCTGCCTGTACTGCTGGCGCTTATTGGAATCTGTTACAACAATTTCTTTGGTGCGGAAACTGAAACGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCa                                                                                <  1:2024458/148‑1 (MQ=255)
                                                                 ccTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGTCCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCa                                                                                >  1:2629264/1‑148 (MQ=255)
                                                                  cTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCaa                                                                               >  2:1944679/1‑148 (MQ=255)
                                                                   tGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAAc                                                                              <  2:1557750/148‑1 (MQ=255)
                                                                   tGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAAc                                                                              >  1:2665604/1‑148 (MQ=255)
                                                                   tGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAAc                                                                              <  2:2665604/148‑1 (MQ=255)
                                                                       gagaAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTa                                                                          <  1:824948/148‑1 (MQ=255)
                                                                       gagaAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTa                                                                          <  1:3075234/148‑1 (MQ=255)
                                                                         gaAAAACCTGCCTGTACTGGTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGCAAACTGACACGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAg                                                                        <  1:1439697/148‑1 (MQ=255)
                                                                          aaaaaCCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGt                                                                       <  2:169399/148‑1 (MQ=255)
                                                                          aaaaaCCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGt                                                                       >  1:1813644/1‑148 (MQ=255)
                                                                          aaaaaCCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGt                                                                       >  1:2227506/1‑148 (MQ=255)
                                                                          aaaaaCCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGt                                                                       >  1:1734821/1‑148 (MQ=255)
                                                                          aaaaaCCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGt                                                                       >  2:2610418/1‑148 (MQ=255)
                                                                          aaaaaCCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGt                                                                       >  1:2380029/1‑148 (MQ=255)
                                                                              aCCTGCCTGTACTGCTGGCGCTGATTGGCATCTGCTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGt                                                                   <  2:3061728/148‑1 (MQ=255)
                                                                                  gcctgTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGAc                                                               >  2:1936513/1‑148 (MQ=255)
                                                                                     tgTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCgt                                                            >  2:321904/1‑148 (MQ=255)
                                                                                      gTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCgta                                                           <  1:398774/148‑1 (MQ=255)
                                                                                      gTACTGCTGGCGCTGATTGGCATCGGGTACAACAATTTCTTGGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCgta                                                           <  1:1039285/148‑1 (MQ=255)
                                                                                      gTACGGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCgta                                                           <  1:1039641/148‑1 (MQ=255)
                                                                                            ctgGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCgtaacggta                                                     <  1:1944527/148‑1 (MQ=255)
                                                                                             tgGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCgtaacggtaa                                                    <  2:2441049/148‑1 (MQ=255)
                                                                                              ggCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCgtaacggtaac                                                   >  1:1853096/1‑148 (MQ=255)
                                                                                              ggCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCgtaacggtaac                                                   >  1:1960480/1‑148 (MQ=255)
                                                                                              ggCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCgtaacggtaac                                                   >  1:1852972/1‑148 (MQ=255)
                                                                                              ggCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCgtaacggtaac                                                   >  1:1960549/1‑148 (MQ=255)
                                                                                                    gATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTtgtg                                             >  1:446272/1‑148 (MQ=255)
                                                                                                       tGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAACGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGAt                                          >  1:1442489/1‑148 (MQ=255)
                                                                                                            tCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAACGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCa                                     >  2:724477/1‑148 (MQ=255)
                                                                                                             cTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAg                                    >  2:360536/1‑148 (MQ=255)
                                                                                                             cTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAg                                    >  2:421417/1‑148 (MQ=255)
                                                                                                                  acaacaATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTgg                               >  1:1578625/1‑148 (MQ=255)
                                                                                                                  acaacaATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTgg                               >  1:1577772/1‑148 (MQ=255)
                                                                                                                   caacaaTTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGc                              >  2:1053203/1‑148 (MQ=255)
                                                                                                                   caacaaTTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGc                              >  2:1121803/1‑148 (MQ=255)
                                                                                                                    aacaaTTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACAAGACTGGcc                             >  1:2877151/1‑148 (MQ=255)
                                                                                                                     acaaTTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGccc                            >  1:1277957/1‑148 (MQ=255)
                                                                                                                        aTTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGAt                         >  2:465319/1‑148 (MQ=255)
                                                                                                                        aTTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGAt                         <  2:2854007/148‑1 (MQ=255)
                                                                                                                           tCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTAt                      >  2:1798422/1‑148 (MQ=255)
                                                                                                                           tCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTAt                      >  2:3239793/1‑148 (MQ=255)
                                                                                                                              ttGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTg                   >  1:586497/1‑148 (MQ=255)
                                                                                                                              ttGGTGCGGAAACTGAAACGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTg                   >  2:2374275/1‑148 (MQ=255)
                                                                                                                              ttGGTGCGGAAACTGAAACGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTg                   >  2:3009541/1‑148 (MQ=255)
                                                                                                                               tGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTgg                  <  1:3040554/148‑1 (MQ=255)
                                                                                                                                  tGCGGAAACTGAAACGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGt               <  2:1041666/148‑1 (MQ=255)
                                                                                                                                  tGCGGAAACTGAAACGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGAGTATCTGGGGt               >  2:1794372/1‑148 (MQ=255)
                                                                                                                                    cGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGa             <  1:2059701/148‑1 (MQ=255)
                                                                                                                                      gAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAAc           >  1:2486669/1‑148 (MQ=255)
                                                                                                                                       aaaCTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAAcc          >  1:993495/1‑148 (MQ=255)
                                                                                                                                       aaaCTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAAcc          >  1:993556/1‑148 (MQ=255)
                                                                                                                                       aaaCTGAAACGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAAcc          <  1:1644072/148‑1 (MQ=255)
                                                                                                                                        aaCTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCa         <  2:2504799/148‑1 (MQ=255)
                                                                                                                                            gAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCa     <  2:2364913/148‑1 (MQ=255)
                                                                                                                                              aGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACt   >  2:1654413/1‑148 (MQ=255)
                                                                                                                                               gCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTa  <  2:2437431/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
GCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTA  >  CP009273/4224542‑4224832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑