Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,225,841 A→C 89.7% K3T (AAA→ACA)  yjbE → extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,225,8410AC89.7% 683.7 / 34.4 199K3T (AAA→ACA) yjbEextracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand):  ref base A (13/8);  new base C (99/79);  total (112/87)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 204 total aligned reads displayed.

TTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAG  >  CP009273/4225696‑4225985
                                                                                                                                                 |                                                                                                                                                
ttGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAg                                                                                                                                                >  2:2090925/1‑148 (MQ=255)
ttGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAg                                                                                                                                                >  2:1564901/1‑148 (MQ=255)
ttGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAg                                                                                                                                                <  1:837306/148‑1 (MQ=255)
 tGATATTCATTCCAATTATCCTAACACGCCATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGt                                                                                                                                               <  2:2474769/148‑1 (MQ=255)
   atatTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTc                                                                                                                                             <  1:496611/148‑1 (MQ=255)
    tatTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCt                                                                                                                                            >  1:953016/1‑148 (MQ=255)
     attcattcCAATTATCCTAAAACGCCATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTg                                                                                                                                           <  1:105001/148‑1 (MQ=255)
       tcattcCAATTATCCTAAAACGCCATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTa                                                                                                                                         >  1:2969396/1‑148 (MQ=255)
        cattcCAATTATCCTAAAACGCCATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTAt                                                                                                                                        <  1:155204/148‑1 (MQ=255)
         agtcCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATg                                                                                                                                       <  2:2861560/146‑1 (MQ=255)
           tcCAATTATCCTAAAACGCCATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGc                                                                                                                                     >  1:1585042/1‑148 (MQ=255)
           tcCAATTATCCTAAAACGCCATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGc                                                                                                                                     >  2:1498562/1‑148 (MQ=255)
           tcCAATTATCCTAAAACGCCATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGc                                                                                                                                     >  1:767128/1‑148 (MQ=255)
           tcCAATTATCCTAAAACGCCATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGc                                                                                                                                     <  1:376487/148‑1 (MQ=255)
           tcCAATTATCCTAAAACGCCATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGc                                                                                                                                     >  1:109584/1‑148 (MQ=255)
            ccAATTATCCTAAAACGCCATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCa                                                                                                                                    <  1:3162833/148‑1 (MQ=255)
            ccAATTATCCTAAAACGCCATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCa                                                                                                                                    <  1:2836579/148‑1 (MQ=255)
             cAATTATCCTAAAACGCCATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCAt                                                                                                                                   >  2:1292090/1‑148 (MQ=255)
              aaTTATCCTAAAACGCCATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCAtt                                                                                                                                  <  2:487450/148‑1 (MQ=255)
                ttATCCTAAAACGCCATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCAtttt                                                                                                                                <  2:2178553/148‑1 (MQ=255)
                ttATCCTAAAACGCCATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCAtttt                                                                                                                                <  2:2178642/148‑1 (MQ=255)
                 tATCCTAAAACGCCATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCAttttt                                                                                                                               >  1:1258038/1‑148 (MQ=255)
                 tATCCTAAAACGCCATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCAttttt                                                                                                                               >  2:2382182/1‑148 (MQ=255)
                 tATCCTAAAACGCCATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCAttttt                                                                                                                               >  2:70822/1‑148 (MQ=255)
                      tAAAACGCCATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCat                                                                                                                          <  2:781946/148‑1 (MQ=255)
                       aaaaCGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATGTTTGCCata                                                                                                                         >  2:3159958/1‑148 (MQ=255)
                        aaaCGCCATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCatat                                                                                                                        <  1:380026/148‑1 (MQ=255)
                         aaCGCCATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATc                                                                                                                       >  2:2754743/1‑148 (MQ=255)
                         aaCGCCATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATc                                                                                                                       >  2:137896/1‑148 (MQ=255)
                         aaCGCCATCGCTAAGTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATc                                                                                                                       <  2:1969477/148‑1 (MQ=255)
                           cGCCATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTg                                                                                                                     >  1:2281107/1‑148 (MQ=255)
                           cGCCATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTg                                                                                                                     >  1:202323/1‑148 (MQ=255)
                             ccATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTgcg                                                                                                                   <  2:1575524/148‑1 (MQ=255)
                              cATCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTgcgc                                                                                                                  <  1:1404710/148‑1 (MQ=255)
                                tCGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCtt                                                                                                                >  1:1596805/1‑148 (MQ=255)
                                 cGCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTg                                                                                                               <  1:1514720/148‑1 (MQ=255)
                                  gCTAATTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTgc                                                                                                              <  2:2534684/148‑1 (MQ=255)
                                     aaTTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTgcggc                                                                                                           >  1:1875047/1‑148 (MQ=255)
                                      aTTCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTgcggcg                                                                                                          >  2:3015042/1‑148 (MQ=255)
                                       ttCCCCTCGCCGTAAGTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGa                                                                                                         >  2:436912/1‑148 (MQ=255)
                                        tCCCCTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGAc                                                                                                        >  2:1501560/1‑148 (MQ=255)
                                         ccccTCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACt                                                                                                       >  1:3268263/1‑148 (MQ=255)
                                           cctcgcCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTc                                                                                                     <  2:857351/145‑1 (MQ=255)
                                              cgcCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGc                                                                                                  >  1:470409/1‑148 (MQ=255)
                                               gcCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCg                                                                                                 <  1:118356/148‑1 (MQ=255)
                                               gcCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCg                                                                                                 >  1:2568103/1‑148 (MQ=255)
                                                ccGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGt                                                                                                <  2:3264242/148‑1 (MQ=255)
                                                 cGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTg                                                                                               <  2:1007388/148‑1 (MQ=255)
                                                  gTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTgg                                                                                              >  2:2985727/1‑148 (MQ=255)
                                                  gTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTgg                                                                                              >  2:170100/1‑148 (MQ=255)
                                                   tAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTggg                                                                                             <  2:3006026/148‑1 (MQ=255)
                                                   tAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTggg                                                                                             <  2:3087943/148‑1 (MQ=255)
                                                   tAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTggg                                                                                             <  1:2520644/148‑1 (MQ=255)
                                                    aaTTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGc                                                                                            >  2:1655215/1‑148 (MQ=255)
                                                        cGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCa                                                                                        <  1:1498562/148‑1 (MQ=255)
                                                         gCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCAc                                                                                       <  1:2798348/148‑1 (MQ=255)
                                                         gCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCAc                                                                                       <  2:1645670/148‑1 (MQ=255)
                                                          cATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCAcc                                                                                      >  2:186188/1‑148 (MQ=255)
                                                             gCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGt                                                                                   >  2:17080/1‑148 (MQ=255)
                                                                ttAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACa                                                                                <  2:1141713/148‑1 (MQ=255)
                                                                   gTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGt                                                                             <  2:2124798/148‑1 (MQ=255)
                                                                   gTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTCTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGt                                                                             <  2:2246707/148‑1 (MQ=255)
                                                                    ttGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTg                                                                            >  2:976129/1‑148 (MQ=255)
                                                                    ttGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTg                                                                            >  1:715315/1‑148 (MQ=255)
                                                                             cTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCg                                                                   >  1:2158857/1‑148 (MQ=255)
                                                                              tCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCgg                                                                  >  1:1309157/1‑148 (MQ=255)
                                                                                            aaTGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGgcagca                                                    <  2:1819580/148‑1 (MQ=255)
                                                                                                   ttGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCgg                                             <  1:3235712/148‑1 (MQ=255)
                                                                                                         aaaTCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTcgg                                       <  1:593338/148‑1 (MQ=255)
                                                                                                          aaTCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTcggc                                      >  1:2171810/1‑148 (MQ=255)
                                                                                                           aTCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTcggcg                                     >  2:270868/1‑148 (MQ=255)
                                                                                                              tcCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCgggg                                  <  2:551185/148‑1 (MQ=255)
                                                                                                              tcCATTCATTCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCgggg                                  <  1:2382182/148‑1 (MQ=255)
                                                                                                               ccATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGa                                 <  1:266478/148‑1 (MQ=255)
                                                                                                                cattcattCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAg                                <  2:2530517/148‑1 (MQ=255)
                                                                                                                cattcattCAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAg                                >  2:605770/1‑148 (MQ=255)
                                                                                                                 attcattcAATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGt                               >  2:1803222/1‑148 (MQ=255)
                                                                                                                   tcattcaATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTtc                             >  1:1022777/1‑148 (MQ=255)
                                                                                                                      ttcaATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTtcctc                          <  1:1791882/148‑1 (MQ=255)
                                                                                                                      ttcaATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTtcctc                          <  2:955027/148‑1 (MQ=255)
                                                                                                                        caATGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCcg                        >  1:2894327/1‑148 (MQ=255)
                                                                                                                           tGAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGa                     <  2:290846/148‑1 (MQ=255)
                                                                                                                            gAAGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGAc                    >  2:84589/1‑148 (MQ=255)
                                                                                                                             aaGGGAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGAcc                   <  2:1585042/148‑1 (MQ=255)
                                                                                                                               gggAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAg                 >  1:415789/1‑148 (MQ=255)
                                                                                                                               gggAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAg                 >  2:1063336/1‑148 (MQ=255)
                                                                                                                                ggAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGc                >  2:351496/1‑148 (MQ=255)
                                                                                                                                ggAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGc                >  1:771786/1‑148 (MQ=255)
                                                                                                                                 gAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCg               >  1:2469762/1‑148 (MQ=255)
                                                                                                                                 gAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCg               >  1:2926843/1‑148 (MQ=255)
                                                                                                                                 gAAATTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCg               >  1:460301/1‑148 (MQ=255)
                                                                                                                                  aaaTTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGt              <  2:1875047/148‑1 (MQ=255)
                                                                                                                                  aaaTTGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGt              >  2:1454553/1‑148 (MQ=255)
                                                                                                                                      tGTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGc          >  2:1773640/1‑148 (MQ=255)
                                                                                                                                       tttattaAAACAGTTATTTTTGTAATTTTTGCCATTTCTGCTCTTGATTCTTCTTATGAGTTGGGTTCACGTTTACATGTGTTGGAAGCGGAATGGTATGATGCATCGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGtgtgca         <  2:402743/147‑6 (MQ=255)
                                                                                                                                       gTTATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCa         <  2:1027235/148‑1 (MQ=255)
                                                                                                                                         tATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCAcc       >  2:498534/1‑148 (MQ=255)
                                                                                                                                         tATGAAAACAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCAcc       >  2:1275245/1‑148 (MQ=255)
                                                                                                                                             aaaacaGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTaa   >  1:2163418/1‑148 (MQ=255)
                                                                                                                                             aaaacaGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTaa   >  1:2162967/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAG  >  CP009273/4225696‑4225985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑