BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,231,784	G→T	9.6%	R133R (CGC→CGA)	xylE ←	D‑xylose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,231,784	0	G	T	9.6%	624.6 / 29.5	183	R133R (CGC→CGA)	xylE	D‑xylose transporter
Reads supporting (aligned to +/- strand): ref base G (87/78); new base T (8/10); total (95/88)
Fisher's exact test for biased strand distribution p-value = 6.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 187 total aligned reads displayed.

GTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGA  >  CP009273/4231637‑4231931

gTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATg                                                                                                                                                     <  2:1276860/148‑1 (MQ=255)

 tttACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGc                                                                                                                                                    >  1:467322/1‑148 (MQ=255)

     cGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGata                                                                                                                                                <  2:1074403/148‑1 (MQ=255)

        aGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGataaat                                                                                                                                             <  1:1879933/148‑1 (MQ=255)

        aGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGataaat                                                                                                                                             <  1:2326794/148‑1 (MQ=255)

         gTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGataaata                                                                                                                                            >  1:3246214/1‑148 (MQ=255)

            aaCTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACa                                                                                                                                         >  1:78857/1‑148 (MQ=255)

               tAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAAt                                                                                                                                      <  1:2955461/148‑1 (MQ=255)

                aaaaGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATTCGATAAATAACAAAtt                                                                                                                                     <  1:307878/148‑1 (MQ=255)

                aaaaGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAAtt                                                                                                                                     >  1:816948/1‑148 (MQ=255)

                aaaaGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAAtt                                                                                                                                     <  2:2590366/148‑1 (MQ=255)

                 aaaGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTc                                                                                                                                    <  1:445107/148‑1 (MQ=255)

                 aaaGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTc                                                                                                                                    >  1:705442/1‑148 (MQ=255)

                  aaGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTcc                                                                                                                                   >  2:946839/1‑148 (MQ=255)

                          cGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATa                                                                                                                           >  2:2981649/1‑148 (MQ=255)

                            aaaaTAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAAc                                                                                                                         >  2:1837386/1‑148 (MQ=255)

                            aaaaTAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAAc                                                                                                                         >  2:3198043/1‑148 (MQ=255)

                            aaaaTAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAAc                                                                                                                         >  2:32666/1‑148 (MQ=255)

                               ataatCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTg                                                                                                                      <  2:621459/148‑1 (MQ=255)

                               ataatCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTg                                                                                                                      >  1:1246777/1‑148 (MQ=255)

                                    cGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGa                                                                                                                 >  2:1738039/1‑148 (MQ=255)

                                     gCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGAt                                                                                                                >  1:2417362/1‑148 (MQ=255)

                                       aaaCTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATaa                                                                                                              <  1:412067/148‑1 (MQ=255)

                                       aaaCTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATaa                                                                                                              >  2:217177/1‑148 (MQ=255)

                                        aaCTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATaaa                                                                                                             <  2:401466/148‑1 (MQ=255)

                                         aCTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAAc                                                                                                            >  1:2506126/1‑148 (MQ=255)

                                         aCTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAAc                                                                                                            >  1:2385270/1‑148 (MQ=255)

                                              ttAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGca                                                                                                       <  2:2577086/148‑1 (MQ=255)

                                                 aaaGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAg                                                                                                    <  1:2580232/148‑1 (MQ=255)

                                                 aaaGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAg                                                                                                    <  1:1529106/148‑1 (MQ=255)

                                                   agagACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAgtg                                                                                                  <  1:600585/148‑1 (MQ=255)

                                                    gagaCCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAgtgt                                                                                                 >  2:524684/1‑148 (MQ=255)

                                                     agaCCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGtgtt                                                                                                <  2:487161/148‑1 (MQ=255)

                                                     agaCCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGtgtt                                                                                                <  2:588590/148‑1 (MQ=255)

                                                           gTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCgg                                                                                          <  2:2362519/148‑1 (MQ=255)

                                                           gTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCgg                                                                                          >  1:1610132/1‑148 (MQ=255)

                                                            tttCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATTCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCggg                                                                                         >  1:3152846/1‑148 (MQ=255)

                                                            tttCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCggg                                                                                         >  2:679286/1‑148 (MQ=255)

                                                            tttCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCggg                                                                                         <  1:1422596/148‑1 (MQ=255)

                                                            tttCCCGCGAATATGAGCTGGAGCAAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCggg                                                                                         <  1:2524447/148‑1 (MQ=255)

                                                             ttCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGt                                                                                        <  1:614199/148‑1 (MQ=255)

                                                                    gAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATaga                                                                                 <  1:946839/148‑1 (MQ=255)

                                                                    gAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATaga                                                                                 <  2:2898728/148‑1 (MQ=255)

                                                                     aaTATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATagag                                                                                <  2:1916163/148‑1 (MQ=255)

                                                                      atatGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAgg                                                                               <  2:2768791/148‑1 (MQ=255)

                                                                        atGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTa                                                                             <  1:1593033/148‑1 (MQ=255)

                                                                         tGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTaa                                                                            >  2:1387534/1‑148 (MQ=255)

                                                                          gAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTaaa                                                                           <  1:1496274/148‑1 (MQ=255)

                                                                                gAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAg                                                                     <  2:684382/148‑1 (MQ=255)

                                                                                      gTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTgg                                                               <  2:3002349/148‑1 (MQ=255)

                                                                                      gTTCCGCAAGATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTgg                                                               <  2:3003310/148‑1 (MQ=255)

                                                                                       ttCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGc                                                              >  1:1064937/1‑148 (MQ=255)

                                                                                       ttCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGc                                                              >  1:1161829/1‑148 (MQ=255)

                                                                                         ccGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGTGATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAATACCAAGTTCTGGCCa                                                            <  2:2311865/148‑1 (MQ=255)

                                                                                           gCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAgg                                                          <  2:78857/148‑1 (MQ=255)

                                                                                             aaTATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCa                                                        >  1:2267589/1‑148 (MQ=255)

                                                                                               tataCATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGa                                                      >  1:2889072/1‑148 (MQ=255)

                                                                                               tataCATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGa                                                      >  1:1369293/1‑148 (MQ=255)

                                                                                                   cATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCt                                                  <  2:1488201/148‑1 (MQ=255)

                                                                                                    aTTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTa                                                 >  1:3096349/1‑148 (MQ=255)

                                                                                                      tGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTaca                                               >  2:1795559/1‑148 (MQ=255)

                                                                                                        gCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACAcc                                             >  1:530183/1‑148 (MQ=255)

                                                                                                        gCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACAcc                                             >  1:2194272/1‑148 (MQ=255)

                                                                                                           agagCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGa                                          >  2:2930629/1‑148 (MQ=255)

                                                                                                            gagCATTGAGGCTAAACCAACGCCAATACCGCCAATAATTCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGaa                                         <  1:273048/148‑1 (MQ=255)

                                                                                                               cATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATa                                      >  1:351856/1‑148 (MQ=255)

                                                                                                               cATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATa                                      >  2:119338/1‑148 (MQ=255)

                                                                                                               cATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATa                                      >  1:2925408/1‑148 (MQ=255)

                                                                                                                 ttGAGGCTAAACCAACGCCAATACCGCCAATAATTCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATaaa                                    <  1:1947241/148‑1 (MQ=255)

                                                                                                                 ttGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATaaa                                    <  2:59210/148‑1 (MQ=255)

                                                                                                                   gAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCTGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATaaaaa                                  <  1:1076066/148‑1 (MQ=255)

                                                                                                                    agtcTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATaaaaaa                                 <  1:2758499/145‑1 (MQ=255)

                                                                                                                        tAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAgg                             <  1:141654/148‑1 (MQ=255)

                                                                                                                         aaaCCAACGCCAATACCGCCAATAATTCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGa                            <  1:1697878/148‑1 (MQ=255)

                                                                                                                         aaaCCAACGCCAATACCGCAAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGa                            <  1:1738039/148‑1 (MQ=255)

                                                                                                                          aaCCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAggcc                           <  2:1246640/148‑3 (MQ=255)

                                                                                                                             cAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTACAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGAcagc                        >  1:3118722/1‑148 (MQ=255)

                                                                                                                               aCGCCAATACCGCCAATAATTCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGAcagcag                      <  2:2819959/148‑1 (MQ=255)

                                                                                                                                cGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACagcagc                     >  1:2087503/1‑148 (MQ=255)

                                                                                                                                 gCCAATACCGCCAATAATTCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAgcagca                    <  1:2351536/148‑1 (MQ=255)

                                                                                                                                 gCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAgcagca                    <  1:1920538/148‑1 (MQ=255)

                                                                                                                                  ccAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCaa                   >  1:2357145/1‑148 (MQ=255)

                                                                                                                                    aaTACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATc                 <  2:2401961/148‑1 (MQ=255)

                                                                                                                                    aaTACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATc                 >  2:871359/1‑148 (MQ=255)

                                                                                                                                     aTACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCt                >  1:3208390/1‑148 (MQ=255)

                                                                                                                                        ccGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTaa             >  1:1645860/1‑148 (MQ=255)

                                                                                                                                         cGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAg            >  1:605687/1‑148 (MQ=255)

                                                                                                                                          gCCAATAATTCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGt           >  2:590185/1‑148 (MQ=255)

                                                                                                                                          cccAATACCGCGATAAATACCAAAGTCCGGGACCCAACCTCCCAGACAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGt           <  1:2767114/147‑1 (MQ=255)

                                                                                                                                           ccAATAATTCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTg          >  2:1268444/1‑148 (MQ=255)

                                                                                                                                            cAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGa         >  2:3115881/1‑148 (MQ=255)

                                                                                                                                             aataatGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACATGACAGCAGCAATCTTAAGTGaa        <  2:1610132/148‑1 (MQ=255)

                                                                                                                                              ataatGCGATACATAACAAATTCCGGGACATAACCTGCCTGATAAACAGGCACAGTGTTGTCCGGGTTTATAGATGTAAAACCAAGTTCTGGACAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAAt       <  2:1981655/148‑1 (MQ=255)

                                                                                                                                              ataatGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAAt       >  1:1322964/1‑148 (MQ=255)

                                                                                                                                              ataatGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAAt       >  2:2059166/1‑148 (MQ=255)

                                                                                                                                               taatTCGATAAATAACAAAGTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATc      <  2:2014934/148‑1 (MQ=255)

                                                                                                                                               taatGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATc      <  1:1795559/148‑1 (MQ=255)

                                                                                                                                               taatGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATc      >  1:2687444/1‑148 (MQ=255)

                                                                                                                                               taatGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATc      <  1:123868/148‑1 (MQ=255)

                                                                                                                                                   gCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCacga  <  2:2506126/148‑1 (MQ=255)

GTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGA  >  CP009273/4231637‑4231931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`