Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,235,869 G→T 91.9% A160E (GCG→GAG)  malE ← maltose transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,235,8690GT91.9% 639.7 / 46.9 240A160E (GCG→GAG) malEmaltose transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (8/12);  new base T (102/117);  total (111/129)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 240 total aligned reads displayed.

GCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCGCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTGTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCC  >  CP009273/4235724‑4236012
                                                                                                                                                 |                                                                                                                                               
  ccACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCgg                                                                                                                                             <  2:1955471/148‑1 (MQ=255)
   cACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCggg                                                                                                                                            >  2:1539521/1‑148 (MQ=255)
   cACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCggg                                                                                                                                            <  1:1049800/148‑1 (MQ=255)
    aCGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGa                                                                                                                                           >  2:954291/1‑148 (MQ=255)
     cGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGAt                                                                                                                                          <  1:2707556/148‑1 (MQ=255)
        cTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTGAGTTCTTTATAAAGCGCCGGGGtctc                                                                                                                                       >  1:1433619/1‑148 (MQ=255)
        cTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGAtctc                                                                                                                                       >  1:276834/1‑148 (MQ=255)
         tttAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCCGCGCGCTCTTACCTTTCGCTTTGAGTTCTTTATACAGCTCCGGGctctct                                                                                                                                      >  1:709724/1‑148 (MQ=255)
         tttAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATctct                                                                                                                                      >  2:2003824/1‑148 (MQ=255)
          ttAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCtt                                                                                                                                     >  2:1486482/1‑148 (MQ=255)
           tAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCGCCGGGATCTCTTc                                                                                                                                    >  2:1956255/1‑148 (MQ=255)
            aaTGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTcc                                                                                                                                   <  1:802300/148‑1 (MQ=255)
            aaTGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTcc                                                                                                                                   <  1:2003824/148‑1 (MQ=255)
            aaTGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTcc                                                                                                                                   <  2:539129/148‑1 (MQ=255)
              tGTCGTACTTGCCGTTTTCCTACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGCTCTCTTcccc                                                                                                                                 >  1:2946645/1‑147 (MQ=255)
              tGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCa                                                                                                                                 <  1:2568795/148‑1 (MQ=255)
                 cGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGt                                                                                                                              <  1:828362/148‑1 (MQ=255)
                   tACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGttt                                                                                                                            >  2:1907349/1‑148 (MQ=255)
                   tACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTAGCTTTCAGTTCTTTATCCAGCTCCGGGATATCTTCCCAGGttt                                                                                                                            >  1:517969/1‑148 (MQ=255)
                    aCTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTCCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGtttt                                                                                                                           <  1:1249938/148‑1 (MQ=255)
                     cTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGttttt                                                                                                                          >  2:1648834/1‑148 (MQ=255)
                      ttGCCGTTTTCATACTTTAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGGTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTg                                                                                                                         >  2:883816/1‑148 (MQ=255)
                       tGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGGTTTTgg                                                                                                                        >  2:3179715/1‑148 (MQ=255)
                         ccGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCg                                                                                                                      <  2:2494403/148‑1 (MQ=255)
                             tttCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATACAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGtt                                                                                                                  >  1:2234214/1‑148 (MQ=255)
                               tCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCg                                                                                                                >  1:119922/1‑148 (MQ=255)
                                 aTACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGgc                                                                                                              >  2:646040/1‑148 (MQ=255)
                                 aTACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGgc                                                                                                              <  2:2240379/148‑1 (MQ=255)
                                       gAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGa                                                                                                        <  1:2853422/148‑1 (MQ=255)
                                        aaCGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGac                                                                                                       <  1:1280190/148‑2 (MQ=255)
                                        aaCGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGAt                                                                                                       <  1:2269065/148‑1 (MQ=255)
                                        aaCGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGAt                                                                                                       <  1:65528/148‑1 (MQ=255)
                                          cGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCt                                                                                                     <  2:2889754/148‑1 (MQ=255)
                                            cATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCATGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCttt                                                                                                   <  1:2169976/148‑1 (MQ=255)
                                             aTAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCtttt                                                                                                  <  2:2082262/148‑2 (MQ=255)
                                                aCCCCCGTCAGCAGCAATCCGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCGTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTta                                                                                               <  1:1562347/148‑1 (MQ=255)
                                                 cccccGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTtat                                                                                              >  1:3042278/1‑148 (MQ=255)
                                                   cccGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATaa                                                                                            <  2:1251391/148‑1 (MQ=255)
                                                   cccGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATaa                                                                                            <  1:570524/148‑1 (MQ=255)
                                                   cccGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATaa                                                                                            <  1:1857113/148‑1 (MQ=255)
                                                       tCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCa                                                                                        <  1:3106932/148‑1 (MQ=255)
                                                       tCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCa                                                                                        <  2:2393399/148‑1 (MQ=255)
                                                        cagcagCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAg                                                                                       >  1:726136/1‑148 (MQ=255)
                                                           cagcaATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATAAGCGa                                                                                    >  2:1912535/1‑148 (MQ=255)
                                                             gcaATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATa                                                                                  <  1:1027161/148‑1 (MQ=255)
                                                             gcaATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCGCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTGTTATAAATCAGCGATa                                                                                  <  2:1031247/148‑1 (MQ=255)
                                                              caATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATaa                                                                                 <  2:2749000/148‑1 (MQ=255)
                                                              caATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATaa                                                                                 <  2:2825197/148‑1 (MQ=255)
                                                              caATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATaa                                                                                 >  2:522928/1‑148 (MQ=255)
                                                              caATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATACAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATaa                                                                                 >  2:355699/1‑148 (MQ=255)
                                                                 tCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCGCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTGTTATAAATCAGCGATAACGc                                                                              <  1:150651/148‑1 (MQ=255)
                                                                     cGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGGGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCa                                                                          >  1:2778882/1‑148 (MQ=255)
                                                                      ggCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCaa                                                                         <  1:2138254/148‑1 (MQ=255)
                                                                      ggCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCaa                                                                         <  2:922497/148‑1 (MQ=255)
                                                                        ccAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACa                                                                       <  1:1530067/148‑1 (MQ=255)
                                                                         cAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAg                                                                      <  2:2711408/148‑1 (MQ=255)
                                                                            gTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCGCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTGTTATAAATCAGCGATAACGCTTCAACAGCGa                                                                   >  1:423727/1‑148 (MQ=255)
                                                                            gTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCGCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTGTTATAAATCAGAGATAACGCTTCAACAGCGa                                                                   >  1:2091617/1‑148 (MQ=255)
                                                                            gTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCGCCGGGATCACATCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCTATAACGCTTCAACAGCGa                                                                   >  1:2912105/1‑148 (MQ=255)
                                                                             tGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGAt                                                                  >  2:3204262/1‑148 (MQ=255)
                                                                                 gTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCggg                                                              <  2:489779/148‑1 (MQ=255)
                                                                                 gTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCggg                                                              <  2:868922/148‑1 (MQ=255)
                                                                                  tACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGt                                                             >  2:2921041/1‑148 (MQ=255)
                                                                                         cTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAAt                                                      >  1:2571120/1‑148 (MQ=255)
                                                                                           tGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCTATAACGCTTCAACAGCGATCGGGTAAGCAATCa                                                    >  1:1003280/1‑148 (MQ=255)
                                                                                           tGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCTATAAAGCTTCAACAGCGATCGGGTAAGCAATCa                                                    >  1:1002830/1‑148 (MQ=255)
                                                                                                gTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTg                                               <  1:1748132/148‑1 (MQ=255)
                                                                                                 ttGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGc                                              <  1:1384217/148‑1 (MQ=255)
                                                                                                 ttGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGc                                              <  1:1384353/148‑1 (MQ=255)
                                                                                                    aaCATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCGCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTGTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGt                                           <  2:1433619/148‑1 (MQ=255)
                                                                                                      cATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTg                                         <  2:1290811/148‑1 (MQ=255)
                                                                                                      cATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTg                                         >  2:798672/1‑148 (MQ=255)
                                                                                                        tCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTa                                       <  1:938287/148‑1 (MQ=255)
                                                                                                         cAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGCTCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATAAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATAAGCTTGCCGGTGTaa                                      >  1:583914/1‑148 (MQ=255)
                                                                                                            cgcgcTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGt                                   <  1:1907349/148‑1 (MQ=255)
                                                                                                            cgcgcTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGt                                   >  1:3018440/1‑148 (MQ=255)
                                                                                                            cgcgcTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGGTGTAACGt                                   >  1:629073/1‑148 (MQ=255)
                                                                                                              cgcTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTAc                                 <  1:997805/148‑1 (MQ=255)
                                                                                                               gcTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACg                                <  1:67642/148‑1 (MQ=255)
                                                                                                                ctctTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACgg                               >  2:2428569/1‑148 (MQ=255)
                                                                                                                 tctTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGc                              >  2:1702878/1‑148 (MQ=255)
                                                                                                                  ctTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCa                             <  2:3255708/148‑1 (MQ=255)
                                                                                                                  ctTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCa                             >  1:1082448/1‑148 (MQ=255)
                                                                                                                    tACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCAta                           >  2:2649916/1‑147 (MQ=255)
                                                                                                                        tttCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAg                       >  1:896110/1‑148 (MQ=255)
                                                                                                                            gCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTaa                   <  1:1648834/148‑1 (MQ=255)
                                                                                                                             cTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTaaa                  >  2:1508108/1‑148 (MQ=255)
                                                                                                                             cTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTaaa                  <  1:1672834/148‑1 (MQ=255)
                                                                                                                              tttCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAAc                 >  1:1605211/1‑148 (MQ=255)
                                                                                                                               ttCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACg                >  2:2553585/1‑148 (MQ=255)
                                                                                                                                tCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACgg               <  2:813683/148‑1 (MQ=255)
                                                                                                                                 cAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGa              >  2:3307137/1‑148 (MQ=255)
                                                                                                                                    ttCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATAc           >  1:2572381/1‑148 (MQ=255)
                                                                                                                                       tttATCCAGCGCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTGTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGc        <  1:1980741/148‑1 (MQ=255)
                                                                                                                                       tttATCCAGCGCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTGTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGc        <  1:1369425/148‑1 (MQ=255)
                                                                                                                                        ttATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCt       >  2:121488/1‑148 (MQ=255)
                                                                                                                                            ccAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTc   >  1:1327930/1‑148 (MQ=255)
                                                                                                                                            ccAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTc   >  1:1326607/1‑148 (MQ=255)
                                                                                                                                            ccAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTc   <  1:3177340/148‑1 (MQ=255)
                                                                                                                                             cAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTcc  <  1:2921041/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCGCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTGTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCC  >  CP009273/4235724‑4236012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑