BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,258,903	G→T	16.8%	S13Y (TCT→TAT)	yjbS ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,258,903	0	G	T	16.8%	682.7 / 85.8	238	S13Y (TCT→TAT)	yjbS	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (104/94); new base T (26/14); total (130/108)
Fisher's exact test for biased strand distribution p-value = 1.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.77e-01

Only 100 of 242 total aligned reads displayed.

AAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGTAATGCTGTATCA  >  CP009273/4258756‑4259049

aaaaaCTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAg                                                                                                                                                    >  1:48439/1‑148 (MQ=255)

  aaaCTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGAt                                                                                                                                                  >  1:2404355/1‑148 (MQ=255)

   aaCTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATg                                                                                                                                                 >  1:2391764/1‑148 (MQ=255)

   aaCTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATg                                                                                                                                                 <  1:1236627/148‑1 (MQ=255)

   aaCTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATg                                                                                                                                                 >  2:1524553/1‑148 (MQ=255)

     cTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTc                                                                                                                                               >  2:263462/1‑148 (MQ=255)

      tttACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCg                                                                                                                                              <  1:1185936/148‑1 (MQ=255)

       ttACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGt                                                                                                                                             <  2:620273/148‑1 (MQ=255)

         aCTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGttt                                                                                                                                           <  2:349828/148‑1 (MQ=255)

           ttGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAATATGTCGTTttg                                                                                                                                         >  2:2964911/1‑148 (MQ=255)

           ttGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTttg                                                                                                                                         <  2:2145047/148‑1 (MQ=255)

            tGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTttgt                                                                                                                                        <  2:705198/148‑1 (MQ=255)

            tGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTttgt                                                                                                                                        <  1:3047743/148‑1 (MQ=255)

              cAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAATATGTCGTTttgttg                                                                                                                                      >  1:616206/1‑148 (MQ=255)

              cAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTttgttg                                                                                                                                      >  2:3018216/1‑148 (MQ=255)

              cAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTttgttg                                                                                                                                      <  1:2137840/148‑1 (MQ=255)

              cAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTttgttg                                                                                                                                      <  1:1561600/148‑1 (MQ=255)

                aTAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCt                                                                                                                                    >  1:828932/1‑148 (MQ=255)

                  aGAACTTTATACTTCTTCTCCAGGTATAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGTCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTAt                                                                                                                                  <  2:2899396/148‑1 (MQ=255)

                    aaCTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTc                                                                                                                                >  2:2198146/1‑148 (MQ=255)

                    aaCTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTc                                                                                                                                <  2:1096405/148‑1 (MQ=255)

                      cTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTATTcac                                                                                                                              <  1:2853935/148‑1 (MQ=255)

                      cTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTATTcac                                                                                                                              >  2:2788424/1‑148 (MQ=255)

                       ttGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTcaca                                                                                                                             >  2:454958/1‑148 (MQ=255)

                       ttGATACTTCTTCTCCAGGTCTAAAAAATATTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATTTCGTTTTGTTGCTATTcaca                                                                                                                             <  2:1508666/148‑1 (MQ=255)

                        tGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACat                                                                                                                            >  1:2242426/1‑148 (MQ=255)

                        tGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACat                                                                                                                            >  2:1633810/1‑148 (MQ=255)

                        tGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACat                                                                                                                            <  1:1498003/148‑1 (MQ=255)

                        tGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGAATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACat                                                                                                                            >  1:1806654/1‑148 (MQ=255)

                           tACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATg                                                                                                                         <  2:2512577/148‑1 (MQ=255)

                             cttcttCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGat                                                                                                                       >  2:1048689/1‑148 (MQ=255)

                             cttcttCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGat                                                                                                                       <  2:995014/148‑1 (MQ=255)

                               tcttctCAAGGTCTAAAAAATCTTTTTCAATTTTTAACTGTGTTTCTATGTATAAAAATAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGatat                                                                                                                     <  1:2661166/148‑1 (MQ=255)

                                  tctcCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATAtt                                                                                                                  >  2:2052786/1‑148 (MQ=255)

                                   ctcCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTATTCACATATGATATATTc                                                                                                                 <  2:582647/148‑1 (MQ=255)

                                         gTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATatca                                                                                                           <  1:909418/148‑1 (MQ=255)

                                         gTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATatca                                                                                                           >  2:2242455/1‑148 (MQ=255)

                                          tCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTATTCACATATGATATATTCATatcat                                                                                                          <  2:3082650/148‑1 (MQ=255)

                                          tCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTAATatcat                                                                                                          >  2:2392690/1‑148 (MQ=255)

                                           cTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATatcatc                                                                                                         >  1:2037464/1‑148 (MQ=255)

                                            tAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTATTCACATATGATATATTCATatcatca                                                                                                        >  2:227557/1‑148 (MQ=255)

                                               aaaaTCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCAtct                                                                                                     >  1:2555905/1‑148 (MQ=255)

                                                 aaTCTATTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTcc                                                                                                   <  2:1199099/148‑1 (MQ=255)

                                                  aTCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCa                                                                                                  >  2:1020419/1‑148 (MQ=255)

                                                    cTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATc                                                                                                >  1:35673/1‑148 (MQ=255)

                                                    cTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATc                                                                                                >  1:765903/1‑148 (MQ=255)

                                                      tttGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATcgc                                                                                              >  1:1315337/1‑148 (MQ=255)

                                                       ttGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATcgcg                                                                                             <  2:2702269/148‑1 (MQ=255)

                                                       ttGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATcgcg                                                                                             <  2:1051059/148‑1 (MQ=255)

                                                        tGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGa                                                                                            >  2:1035835/1‑148 (MQ=255)

                                                         gCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAg                                                                                           <  1:99043/148‑1 (MQ=255)

                                                              ttttAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGtaata                                                                                      >  1:2360053/1‑148 (MQ=255)

                                                                     tgtgTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATTATATATTCATATCATCATATCCATCGCGAGTAATAAAATTaa                                                                               >  2:2678448/1‑148 (MQ=255)

                                                                     tgtgTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTaa                                                                               >  2:2479505/1‑148 (MQ=255)

                                                                      gtgtTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAAt                                                                              >  2:84094/1‑148 (MQ=255)

                                                                        gtTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCa                                                                            >  1:3263986/1‑148 (MQ=255)

                                                                         tttCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCAc                                                                           <  1:2964911/148‑1 (MQ=255)

                                                                         tttCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCAc                                                                           >  1:875723/1‑148 (MQ=255)

                                                                          ttCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCAcc                                                                          >  1:1749135/1‑148 (MQ=255)

                                                                           tCTATTTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCa                                                                         <  1:250790/148‑1 (MQ=255)

                                                                             tATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCAtt                                                                       >  2:3233434/1‑148 (MQ=255)

                                                                              aTGTATAAACAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTg                                                                      <  2:3192049/148‑1 (MQ=255)

                                                                               tGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTgt                                                                     >  1:2819784/1‑148 (MQ=255)

                                                                               tGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTgt                                                                     >  2:2714918/1‑148 (MQ=255)

                                                                                 tataAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTgtag                                                                   <  2:543652/148‑1 (MQ=255)

                                                                                 tataAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTggag                                                                   >  1:3004107/1‑148 (MQ=255)

                                                                                  ataAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTgtagg                                                                  >  1:1023944/1‑148 (MQ=255)

                                                                                  ataAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTgtagg                                                                  >  1:2677361/1‑148 (MQ=255)

                                                                                   taAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTgtaggg                                                                 <  2:701092/148‑1 (MQ=255)

                                                                                    aaaaaGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTgtagggt                                                                >  1:705571/1‑148 (MQ=255)

                                                                                       aaGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTgtagggtagg                                                             <  2:2214985/148‑1 (MQ=255)

                                                                                        aGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGtagggtaggg                                                            >  1:1040136/1‑148 (MQ=255)

                                                                                         gAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGTTAAATATGTCGTTTTGTTGCTATTCACATATGATATATTAATATCATCATCTCCATCGCGAGTAATAAAATTAATCAACATTGTAGGGTAgggg                                                           >  2:2357131/1‑148 (MQ=255)

                                                                                          aaaTCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAggggg                                                          >  2:912587/1‑148 (MQ=255)

                                                                                          aaaTCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAggggg                                                          <  1:263462/148‑1 (MQ=255)

                                                                                                   gCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAAt                                                 >  2:1948260/1‑148 (MQ=255)

                                                                                                    cATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATc                                                <  1:2674276/148‑1 (MQ=255)

                                                                                                        agagATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGaa                                            <  2:1256973/148‑1 (MQ=255)

                                                                                                            atatCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCAAATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAatca                                        >  2:1751323/1‑148 (MQ=255)

                                                                                                             tatCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAatcat                                       <  2:733698/148‑1 (MQ=255)

                                                                                                              atCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAatcatc                                      >  1:1647/1‑148 (MQ=255)

                                                                                                                  ttattaTTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTta                                  >  2:2585326/1‑148 (MQ=255)

                                                                                                                   tattatTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTtat                                 >  1:2205646/1‑148 (MQ=255)

                                                                                                                    attattTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTtata                                >  2:2111810/1‑148 (MQ=255)

                                                                                                                        tttAGTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTTGTCAATCAGAAATCATCTTTATAAACt                            >  2:2829716/1‑148 (MQ=255)

                                                                                                                        tttAGTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACt                            >  2:3126584/1‑148 (MQ=255)

                                                                                                                        tttAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACt                            <  1:3132122/148‑1 (MQ=255)

                                                                                                                        tttAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACt                            <  1:1947963/148‑1 (MQ=255)

                                                                                                                         ttAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACtt                           >  2:2296098/1‑148 (MQ=255)

                                                                                                                            gTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGa                        <  1:732225/148‑1 (MQ=255)

                                                                                                                             tGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGAt                       <  2:2252448/148‑1 (MQ=255)

                                                                                                                              gCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGAtt                      <  1:2585949/148‑1 (MQ=255)

                                                                                                                                  cTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGttt                  >  1:662381/1‑148 (MQ=255)

                                                                                                                                    aGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGttttt                <  1:147982/148‑1 (MQ=255)

                                                                                                                                          aCAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGTAATg          <  1:861836/148‑1 (MQ=255)

                                                                                                                                          aCAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGTAATg          <  1:761834/148‑1 (MQ=255)

                                                                                                                                             ggTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGTAATGCTg       >  1:1629070/1‑148 (MQ=255)

                                                                                                                                                aaaTATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGTAATGCTGTAt    >  2:1473396/1‑148 (MQ=255)

                                                                                                                                                  aGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGTAATGCTGTATCa  <  1:3018332/148‑1 (MQ=255)

                                                                                                                                                  aGATGTCGTTTTGTTGCTATTCACATATGATATATTCAGATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGTAATGCTGTATCa  <  2:685127/148‑1 (MQ=255)

AAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGTAATGCTGTATCA  >  CP009273/4258756‑4259049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`