Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,274,460 C→A 12.1% R124L (CGT→CTT)  actP ← acetate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,274,4600CA12.1% 598.6 / 41.1 181R124L (CGT→CTT) actPacetate transporter
Reads supporting (aligned to +/- strand):  ref base C (81/78);  new base A (12/10);  total (93/88)
Fisher's exact test for biased strand distribution p-value = 8.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 181 total aligned reads displayed.

TCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCG  >  CP009273/4274314‑4274606
                                                                                                                                                  |                                                                                                                                                  
tCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACg                                                                                                                                                   >  2:342758/1‑148 (MQ=255)
tCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACg                                                                                                                                                   <  1:9189/148‑1 (MQ=255)
     ttACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCgg                                                                                                                                              >  1:206515/1‑148 (MQ=255)
     ttACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCgg                                                                                                                                              >  1:2152319/1‑148 (MQ=255)
     ttACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCgg                                                                                                                                              >  1:1875898/1‑148 (MQ=255)
       aCCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCa                                                                                                                                            <  1:834569/148‑1 (MQ=255)
         cTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACCTCGGGAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAAt                                                                                                                                          >  1:1079950/1‑148 (MQ=255)
           gcgcCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGACTACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCa                                                                                                                                        <  1:811853/148‑1 (MQ=255)
           gcgcCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCa                                                                                                                                        >  2:1093051/1‑148 (MQ=255)
           gcgcCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGGTCGGCAATCa                                                                                                                                        >  2:1092893/1‑148 (MQ=255)
            cgcCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAg                                                                                                                                       <  2:1996128/148‑1 (MQ=255)
                caccaTCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTCAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGAAGTTCGGCAATCAGGAAc                                                                                                                                   >  2:1733838/1‑148 (MQ=255)
                caccaTCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAAc                                                                                                                                   >  1:628231/1‑148 (MQ=255)
                 accaTCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACa                                                                                                                                  >  1:3194277/1‑148 (MQ=255)
                   caTCTGGGCGATACGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACaaa                                                                                                                                <  1:928607/148‑1 (MQ=255)
                   caTCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACaaa                                                                                                                                >  1:2995943/1‑148 (MQ=255)
                   caTCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACaaa                                                                                                                                <  1:928955/148‑1 (MQ=255)
                   caTCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACaaa                                                                                                                                >  1:1925174/1‑148 (MQ=255)
                   caTCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACaaa                                                                                                                                >  2:2683981/1‑148 (MQ=255)
                    aTCTGGGCGATACGGTAAAGCGCCACCACCCCCAGAGACCCACAGGCCGACAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACCTCGGCAAAGGTGTAGCGCCCCAGGTGACGCAGACGTTCGGCAATCAGGAACaaaa                                                                                                                               <  2:1595557/148‑1 (MQ=255)
                    aTCAGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGATTCAGACGCTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACaaaa                                                                                                                               <  2:1226899/148‑1 (MQ=255)
                     tCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAAt                                                                                                                              <  2:381797/148‑1 (MQ=255)
                      cTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATg                                                                                                                             >  1:2866449/1‑148 (MQ=255)
                       tGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGa                                                                                                                            <  2:2533992/148‑1 (MQ=255)
                            gATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGc                                                                                                                       <  1:383220/148‑1 (MQ=255)
                                 ggTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCACTCAGGAACAAAATGATCGGCCAGcc                                                                                                                  >  2:601895/1‑148 (MQ=255)
                                 ggTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGcc                                                                                                                  >  2:601798/1‑148 (MQ=255)
                                  gTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGccc                                                                                                                 >  1:3272770/1‑148 (MQ=255)
                                    aaaGCGCCACCACCACCAGAGACCCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCccac                                                                                                               >  1:1518673/1‑148 (MQ=255)
                                          ccaccaccaccaGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGaa                                                                                                         >  2:2572968/1‑148 (MQ=255)
                                            accaccaccaGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGc                                                                                                       <  2:2507092/148‑1 (MQ=255)
                                             ccaccaccaGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGcc                                                                                                      <  1:1587375/148‑1 (MQ=255)
                                              caccaccaGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGccc                                                                                                     <  2:2106825/148‑1 (MQ=255)
                                              caccaccaGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGccc                                                                                                     >  1:3301613/1‑148 (MQ=255)
                                              caccaccaGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGccc                                                                                                     >  1:772093/1‑148 (MQ=255)
                                              caccaccaGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGAAGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGccc                                                                                                     >  1:172688/1‑148 (MQ=255)
                                                ccaccaGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGAAGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAg                                                                                                   <  1:1135549/148‑1 (MQ=255)
                                                ccaccaGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGAAGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAg                                                                                                   <  1:1135531/148‑1 (MQ=255)
                                                  accaGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCg                                                                                                 <  2:3159851/148‑1 (MQ=255)
                                                    caGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCGCAGGTTACGCAGACGTTCGGCAATCAGGACCAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAg                                                                                               >  1:1595723/1‑148 (MQ=255)
                                                    caGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAg                                                                                               <  2:613174/148‑1 (MQ=255)
                                                    caGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAg                                                                                               <  2:3135375/148‑1 (MQ=255)
                                                    caGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAg                                                                                               <  2:2274102/148‑1 (MQ=255)
                                                           ccACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTa                                                                                        <  1:1462605/148‑1 (MQ=255)
                                                             acaGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAg                                                                                      <  2:519341/148‑1 (MQ=255)
                                                             acaGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAg                                                                                      <  2:323595/148‑1 (MQ=255)
                                                                  ccGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTc                                                                                 <  1:771757/148‑1 (MQ=255)
                                                                  ccGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTc                                                                                 <  1:2433717/148‑1 (MQ=255)
                                                                  ccGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTc                                                                                 <  1:1269369/148‑1 (MQ=255)
                                                                    gAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCAt                                                                               <  2:1624607/148‑1 (MQ=255)
                                                                    gAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCAt                                                                               <  1:454007/148‑1 (MQ=255)
                                                                     aaaGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATa                                                                              >  1:563746/1‑148 (MQ=255)
                                                                      aaGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGAAGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAg                                                                             <  1:652792/148‑1 (MQ=255)
                                                                       aGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGc                                                                            <  2:2067656/148‑1 (MQ=255)
                                                                       aGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGc                                                                            >  2:2882881/1‑148 (MQ=255)
                                                                           tACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGa                                                                        <  1:342758/148‑1 (MQ=255)
                                                                             cGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAgg                                                                      <  1:2369430/148‑1 (MQ=255)
                                                                             cGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAgg                                                                      <  1:95325/148‑1 (MQ=255)
                                                                               aagcGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTa                                                                    <  2:1693062/145‑1 (MQ=255)
                                                                                aTCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGAAGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTaa                                                                   <  2:2689602/148‑1 (MQ=255)
                                                                                  cGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCCTCATAGCCGGAGGTaaaa                                                                 >  1:1022469/1‑147 (MQ=255)
                                                                                     cccTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACAcc                                                              <  2:563746/148‑1 (MQ=255)
                                                                                     cccTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACAcc                                                              <  1:2870087/148‑1 (MQ=255)
                                                                                     cccTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACAcc                                                              <  2:812779/148‑1 (MQ=255)
                                                                                       cTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGAAGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAg                                                            >  1:2343477/1‑148 (MQ=255)
                                                                                        ttGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAgc                                                           >  2:640997/1‑148 (MQ=255)
                                                                                         tGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAgcg                                                          >  2:2431124/1‑148 (MQ=255)
                                                                                           tttCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGcgcg                                                        >  2:34507/1‑148 (MQ=255)
                                                                                           tttCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGAAGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGcgcg                                                        >  2:2814620/1‑148 (MQ=255)
                                                                                            ttCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCgg                                                       >  2:2267484/1‑148 (MQ=255)
                                                                                              cAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGgag                                                     <  2:3223368/148‑1 (MQ=255)
                                                                                              cAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGgag                                                     >  2:2256830/1‑148 (MQ=255)
                                                                                                    gTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATcccc                                               >  1:2899896/1‑148 (MQ=255)
                                                                                                        gagGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGa                                           >  1:1433029/1‑148 (MQ=255)
                                                                                                           gCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGAAGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATg                                        <  1:2320445/148‑1 (MQ=255)
                                                                                                                    gCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGcggcgg                               <  2:221242/148‑1 (MQ=255)
                                                                                                                     cAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGa                              >  1:1053579/1‑148 (MQ=255)
                                                                                                                     cAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGa                              >  1:1054897/1‑148 (MQ=255)
                                                                                                                     cAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGa                              >  1:1098870/1‑148 (MQ=255)
                                                                                                                       aaGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACa                            >  2:855458/1‑148 (MQ=255)
                                                                                                                        aGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACat                           >  2:1856806/1‑148 (MQ=255)
                                                                                                                        aGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACat                           >  1:2260891/1‑148 (MQ=255)
                                                                                                                        aGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACat                           >  1:2419216/1‑148 (MQ=255)
                                                                                                                         ggTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACata                          >  2:2471239/1‑148 (MQ=255)
                                                                                                                          gtgtAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACatat                         >  1:3008594/1‑148 (MQ=255)
                                                                                                                             tAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGt                      <  1:2473794/148‑1 (MQ=255)
                                                                                                                             tAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGt                      <  2:308389/148‑1 (MQ=255)
                                                                                                                              aGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTc                     <  2:1378/148‑1 (MQ=255)
                                                                                                                                     cAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCa              >  1:256856/1‑148 (MQ=255)
                                                                                                                                      aGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCaa             >  2:2984331/1‑148 (MQ=255)
                                                                                                                                      aGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCaa             <  2:3301613/148‑1 (MQ=255)
                                                                                                                                      aGGTTACGCAGAAGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCaa             >  1:1398368/1‑148 (MQ=255)
                                                                                                                                      aGGTTACGCAGAAGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCaa             >  1:1191303/1‑148 (MQ=255)
                                                                                                                                       ggTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAAt            <  1:1870016/148‑1 (MQ=255)
                                                                                                                                         ttACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCg          >  1:1669928/1‑148 (MQ=255)
                                                                                                                                           aCGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGcc        <  1:1237306/148‑1 (MQ=255)
                                                                                                                                            cGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCa       >  1:578550/1‑148 (MQ=255)
                                                                                                                                            cGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCa       >  1:2059323/1‑148 (MQ=255)
                                                                                                                                            cGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCa       >  1:2059107/1‑148 (MQ=255)
                                                                                                                                                gACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGccc   >  2:1025199/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCG  >  CP009273/4274314‑4274606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑