Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,274,926 A→C 100% I72M (ATT→ATG)  yjcH ← DUF485 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,274,9260AC100.0% 911.7 / NA 224I72M (ATT→ATG) yjcHDUF485 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (130/94);  total (130/94)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 224 total aligned reads displayed.

TTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCA  >  CP009273/4274781‑4275067
                                                                                                                                                 |                                                                                                                                             
ttAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGGCGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCAt                                                                                                                                             >  2:1360485/1‑148 (MQ=255)
   gCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCAc                                                                                                                                          >  2:615078/1‑148 (MQ=255)
      gCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTcc                                                                                                                                       >  1:1312229/1‑148 (MQ=255)
           aGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTTGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAacac                                                                                                                                  >  2:2747922/1‑148 (MQ=255)
             ggAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCa                                                                                                                                <  1:943919/148‑1 (MQ=255)
              gAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCCTGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCaa                                                                                                                               <  1:1622857/148‑1 (MQ=255)
              gAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCaa                                                                                                                               >  1:2206065/1‑148 (MQ=255)
              gAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCaa                                                                                                                               >  1:2206040/1‑148 (MQ=255)
              gAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCaa                                                                                                                               >  1:2056567/1‑148 (MQ=255)
              gAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCaa                                                                                                                               >  1:3021684/1‑148 (MQ=255)
               aGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAAt                                                                                                                              <  1:87379/148‑1 (MQ=255)
               aGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAAt                                                                                                                              <  1:410206/148‑1 (MQ=255)
                gtgtGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAAtt                                                                                                                             <  1:2791881/148‑1 (MQ=255)
                    ggcggcAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGaa                                                                                                                         <  1:799792/148‑1 (MQ=255)
                    ggcggcAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGaa                                                                                                                         <  1:2459264/148‑1 (MQ=255)
                     gcggcAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAAt                                                                                                                        >  1:2001061/1‑148 (MQ=255)
                      cgtcAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACAGGTGAGAACAAAGTAGATCACCATCACTCCAACACCAATTGGAATa                                                                                                                       <  1:38986/145‑1 (MQ=255)
                        gcAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGACCAAAGGAGATCACCATCACTCCAACACCAATTGGAATAcc                                                                                                                     >  2:3105063/1‑148 (MQ=255)
                        gcAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATAcc                                                                                                                     >  2:2237543/1‑148 (MQ=255)
                              gcCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCgtgt                                                                                                               <  2:476849/148‑1 (MQ=255)
                               ccGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGtgtg                                                                                                              <  2:978195/148‑1 (MQ=255)
                                   cAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGc                                                                                                          >  1:89121/1‑148 (MQ=255)
                                    aGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCt                                                                                                         >  2:833230/1‑148 (MQ=255)
                                    aGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCt                                                                                                         >  2:1197278/1‑148 (MQ=255)
                                      aaCTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTgg                                                                                                       >  1:1480547/1‑148 (MQ=255)
                                       aCTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGgt                                                                                                      <  2:1385304/148‑1 (MQ=255)
                                        ctctTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGgtg                                                                                                     >  2:544906/1‑148 (MQ=255)
                                        ctctTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGgtg                                                                                                     >  2:2026480/1‑148 (MQ=255)
                                           ttttCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTc                                                                                                  >  1:1075305/1‑148 (MQ=255)
                                           ttttCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTc                                                                                                  >  1:1738827/1‑148 (MQ=255)
                                            tttCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCg                                                                                                 >  2:339343/1‑148 (MQ=255)
                                                 tgatgCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTc                                                                                            >  1:1880644/1‑148 (MQ=255)
                                                  gatgCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCa                                                                                           >  2:126793/1‑148 (MQ=255)
                                                        tGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAgcggcg                                                                                     >  1:3181026/1‑148 (MQ=255)
                                                        tGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAgcggcg                                                                                     <  1:775628/148‑1 (MQ=255)
                                                        tGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAgcggcg                                                                                     >  2:3146865/1‑148 (MQ=255)
                                                           aCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGc                                                                                  >  1:1583305/1‑148 (MQ=255)
                                                            ccTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGcc                                                                                 <  1:768337/148‑1 (MQ=255)
                                                             cTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGccc                                                                                <  1:459142/148‑1 (MQ=255)
                                                              tCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCcca                                                                               >  1:681246/1‑148 (MQ=255)
                                                                aTGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCccagc                                                                             >  1:3301828/1‑148 (MQ=255)
                                                                aTGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCccagc                                                                             >  2:22019/1‑148 (MQ=255)
                                                                aTGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCccagc                                                                             <  2:456774/148‑1 (MQ=255)
                                                                       aCTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCgg                                                                      >  1:1608818/1‑148 (MQ=255)
                                                                        gttCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCggg                                                                     >  1:2887929/2‑148 (MQ=255)
                                                                        cTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCggg                                                                     <  2:851572/148‑1 (MQ=255)
                                                                          tCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGgcg                                                                   >  2:1097805/1‑148 (MQ=255)
                                                                             ttattaAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGa                                                                >  2:1959352/1‑148 (MQ=255)
                                                                              tattaAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGaa                                                               >  2:2787461/1‑148 (MQ=255)
                                                                              tattaAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGaa                                                               >  1:2492102/1‑148 (MQ=255)
                                                                              tattaAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGaa                                                               <  1:2308910/148‑1 (MQ=255)
                                                                              tattaAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGGGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGaa                                                               >  1:1138417/1‑148 (MQ=255)
                                                                               attaAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAg                                                              >  2:467772/1‑148 (MQ=255)
                                                                               attaAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATGGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAg                                                              <  2:2989818/148‑1 (MQ=255)
                                                                                  aaGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCg                                                           <  1:337095/148‑1 (MQ=255)
                                                                                    gACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGAt                                                         >  2:295558/1‑148 (MQ=255)
                                                                                     aCGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATc                                                        >  1:382318/1‑148 (MQ=255)
                                                                                       ggTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAg                                                      <  2:1112667/148‑1 (MQ=255)
                                                                                         tCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTa                                                    <  2:2280243/148‑1 (MQ=255)
                                                                                           gAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTaaa                                                  <  1:2952904/148‑1 (MQ=255)
                                                                                           gAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTaaa                                                  <  2:1897560/148‑1 (MQ=255)
                                                                                           gAATTCGCCGTTCGCCCGCCAGATGTAGAGACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTaaa                                                  <  2:3181026/148‑1 (MQ=255)
                                                                                            aagtCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAGAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTaaaa                                                 <  2:1786129/145‑1 (MQ=255)
                                                                                             aTTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAAt                                                >  1:1111553/1‑148 (MQ=255)
                                                                                               tCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATaa                                              >  2:1619854/1‑148 (MQ=255)
                                                                                               tCGCCGTTCGCCCGCCAGATGTAGAGACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATaa                                              <  2:3171859/148‑1 (MQ=255)
                                                                                                 gCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAg                                            <  1:1382255/148‑1 (MQ=255)
                                                                                                 gCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAg                                            <  2:599704/148‑1 (MQ=255)
                                                                                                  ccGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGc                                           <  2:459060/148‑1 (MQ=255)
                                                                                                  ccGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGc                                           <  1:562127/148‑1 (MQ=255)
                                                                                                  ccGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGc                                           <  2:1865637/148‑1 (MQ=255)
                                                                                                  ccGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGc                                           >  1:244595/1‑148 (MQ=255)
                                                                                                  ccGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGc                                           >  1:984932/1‑148 (MQ=255)
                                                                                                     ttCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGa                                        >  1:1975994/1‑148 (MQ=255)
                                                                                                      tCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGACGGCGATCAGTAAAATAAAGCCGat                                       >  2:632342/1‑148 (MQ=255)
                                                                                                      tCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGat                                       >  2:306920/1‑148 (MQ=255)
                                                                                                      tCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGat                                       >  2:2960511/1‑148 (MQ=255)
                                                                                                      tCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGat                                       >  2:2460413/1‑148 (MQ=255)
                                                                                                      tCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGat                                       >  2:1049087/1‑148 (MQ=255)
                                                                                                           cgccAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATaaa                                  <  1:3205952/148‑1 (MQ=255)
                                                                                                            gccAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAAc                                 <  2:2056567/148‑1 (MQ=255)
                                                                                                            gcaaGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGTATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAAc                                 <  2:1546083/145‑1 (MQ=255)
                                                                                                             ccAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACt                                >  2:715811/1‑148 (MQ=255)
                                                                                                                  tGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAg                           >  1:1092863/1‑148 (MQ=255)
                                                                                                                   gTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGc                          <  1:142269/148‑1 (MQ=255)
                                                                                                                     aGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCat                        <  1:700638/148‑1 (MQ=255)
                                                                                                                      gATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCata                       >  1:2366873/1‑148 (MQ=255)
                                                                                                                         aCCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCataata                    >  2:2581144/1‑148 (MQ=255)
                                                                                                                         aCCGGTGAGAACAAAGGAGATCACCATCACTCCAACACAAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAATCCGATATAAACTGCCAGCataata                    >  1:2721885/1‑148 (MQ=255)
                                                                                                                             gTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCg                <  2:89121/148‑1 (MQ=255)
                                                                                                                              tGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCGGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGa               >  1:403029/1‑148 (MQ=255)
                                                                                                                                 gaACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAg            >  1:634699/1‑148 (MQ=255)
                                                                                                                                     aaaGGAGATCACCATCACTCCAACACCAACTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGAtg        >  1:2768783/1‑148 (MQ=255)
                                                                                                                                       aGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGAtggt      >  1:35040/1‑148 (MQ=255)
                                                                                                                                       aGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGAtggt      <  1:1638096/148‑1 (MQ=255)
                                                                                                                                        ggAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATCATCGACAGGAtggtg     >  2:2704184/1‑148 (MQ=255)
                                                                                                                                        ggAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGAtggtg     >  2:835915/1‑148 (MQ=255)
                                                                                                                                         gagaTCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGAtggtgg    >  1:1911804/1‑148 (MQ=255)
                                                                                                                                          ataTCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGTATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACGGCCAGCATAATAATCGACAGGATGGTGGc   <  1:3288950/146‑1 (MQ=255)
                                                                                                                                           gaTCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCa  >  1:1173860/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
TTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCA  >  CP009273/4274781‑4275067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑