Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,297,485 G→C 91.6% G44G (GGC→GGG)  alsK ← D‑allose kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,297,4850GC91.6% 693.1 / 43.2 187G44G (GGC→GGG) alsKD‑allose kinase
Reads supporting (aligned to +/- strand):  ref base G (10/6);  new base C (75/96);  total (85/102)
Fisher's exact test for biased strand distribution p-value = 1.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 188 total aligned reads displayed.

CGCTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGCT  >  CP009273/4297341‑4297632
                                                                                                                                                |                                                                                                                                                   
cgcTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCCTCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCg                                                                                                                                                  >  2:71077/1‑148 (MQ=255)
cgcTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCg                                                                                                                                                  >  2:3290399/1‑148 (MQ=255)
  cTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCggg                                                                                                                                                >  2:1806557/1‑148 (MQ=255)
  cTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCggg                                                                                                                                                >  1:2790255/1‑148 (MQ=255)
    gTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAg                                                                                                                                              >  1:397760/1‑148 (MQ=255)
     ttAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGc                                                                                                                                             <  1:2870196/148‑1 (MQ=255)
     ttAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGc                                                                                                                                             >  2:2001705/1‑148 (MQ=255)
      tAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCa                                                                                                                                            <  2:279678/148‑1 (MQ=255)
      tAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCa                                                                                                                                            >  2:1920884/1‑148 (MQ=255)
         cGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATg                                                                                                                                         >  1:1103342/1‑148 (MQ=255)
          ggCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGa                                                                                                                                        >  2:568349/1‑148 (MQ=255)
             aGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACtt                                                                                                                                     <  2:1318848/148‑1 (MQ=255)
              ggTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTc                                                                                                                                    <  1:991243/148‑1 (MQ=255)
              ggTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTc                                                                                                                                    <  2:1979076/148‑1 (MQ=255)
              ggTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTc                                                                                                                                    <  1:1761615/148‑1 (MQ=255)
               gTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCt                                                                                                                                   >  1:2683790/1‑148 (MQ=255)
                ttAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTg                                                                                                                                  <  1:9774/148‑1 (MQ=255)
                ttAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTg                                                                                                                                  <  2:236723/148‑1 (MQ=255)
                 tAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGc                                                                                                                                 >  2:2056705/1‑148 (MQ=255)
                     cGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTc                                                                                                                             >  2:2786006/1‑148 (MQ=255)
                       tAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCg                                                                                                                           >  1:679059/1‑148 (MQ=255)
                        aGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGc                                                                                                                          <  2:1707646/148‑1 (MQ=255)
                        aGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGc                                                                                                                          <  2:1707645/148‑1 (MQ=255)
                        aGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGc                                                                                                                          >  1:2918331/1‑148 (MQ=255)
                         gAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCt                                                                                                                         <  1:1686499/148‑1 (MQ=255)
                            ataatGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCtttt                                                                                                                      <  2:2193478/148‑1 (MQ=255)
                            ataatGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCtttt                                                                                                                      >  1:1995938/1‑148 (MQ=255)
                            ataatGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCAGTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCtttt                                                                                                                      <  1:2496233/148‑1 (MQ=255)
                             taatGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCttttt                                                                                                                     <  1:1806557/148‑1 (MQ=255)
                               ttGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACGAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCttttttt                                                                                                                   <  2:1942389/147‑1 (MQ=255)
                               atGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCTAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCttttttt                                                                                                                   <  2:1511571/148‑1 (MQ=255)
                               atGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCttttttt                                                                                                                   >  2:2908740/1‑148 (MQ=255)
                               atGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCttttttt                                                                                                                   <  2:1173894/148‑1 (MQ=255)
                                   tGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCa                                                                                                               >  1:535331/1‑148 (MQ=255)
                                    gCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAg                                                                                                              >  2:2377063/1‑148 (MQ=255)
                                    gCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAg                                                                                                              <  1:295575/148‑1 (MQ=255)
                                     cGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAgt                                                                                                             <  1:779050/148‑1 (MQ=255)
                                        ttATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTa                                                                                                          <  2:2469490/148‑1 (MQ=255)
                                         tATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAg                                                                                                         >  1:3235720/1‑148 (MQ=255)
                                          aTCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGc                                                                                                        >  2:3207032/1‑148 (MQ=255)
                                          aTCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGc                                                                                                        <  1:757997/148‑1 (MQ=255)
                                            cTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGt                                                                                                      >  1:3137958/1‑148 (MQ=255)
                                              ttACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGttt                                                                                                    >  2:695680/1‑148 (MQ=255)
                                               tACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTc                                                                                                   <  1:1887375/148‑1 (MQ=255)
                                                aCTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCa                                                                                                  >  1:1400432/1‑148 (MQ=255)
                                                 cTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCAc                                                                                                 <  2:33201/148‑1 (MQ=255)
                                                    aCCCGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCtt                                                                                              <  2:2143464/148‑1 (MQ=255)
                                                    aCCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCtt                                                                                              <  1:2532952/148‑1 (MQ=255)
                                                    aCCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCtt                                                                                              >  2:1194593/1‑148 (MQ=255)
                                                       aGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTctg                                                                                           <  1:3013866/148‑1 (MQ=255)
                                                        gcgcCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTctgc                                                                                          >  1:3188349/1‑148 (MQ=255)
                                                        gcgcCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTctgc                                                                                          >  1:2823699/1‑148 (MQ=255)
                                                         cgcCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTctgct                                                                                         >  1:2031131/1‑148 (MQ=255)
                                                           ccGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGt                                                                                       <  2:141718/148‑1 (MQ=255)
                                                              gAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCg                                                                                    >  1:859965/1‑148 (MQ=255)
                                                               aaaTCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGc                                                                                   <  2:3259826/148‑1 (MQ=255)
                                                                         aCCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGc                                                                         <  1:2728525/148‑1 (MQ=255)
                                                                         aCCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGc                                                                         <  1:1641412/148‑1 (MQ=255)
                                                                           cAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCgg                                                                       >  1:1067153/1‑148 (MQ=255)
                                                                             gACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGat                                                                     >  2:814360/1‑148 (MQ=255)
                                                                              aCCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTATTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGata                                                                    <  1:1012647/148‑1 (MQ=255)
                                                                                   gACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGt                                                               <  1:3290399/148‑1 (MQ=255)
                                                                                     cAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTg                                                             >  2:2953877/1‑148 (MQ=255)
                                                                                          agcgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGccccc                                                        >  1:1713668/1‑148 (MQ=255)
                                                                                            cgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCat                                                      <  1:2186489/148‑1 (MQ=255)
                                                                                            cgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCat                                                      >  1:2652956/1‑148 (MQ=255)
                                                                                            cgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCat                                                      <  1:523724/148‑1 (MQ=255)
                                                                                              ttAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCatat                                                    >  1:970130/1‑148 (MQ=255)
                                                                                               tAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATc                                                   >  1:910049/1‑148 (MQ=255)
                                                                                                 aaGCGCCTGAGTTGCTCGTGAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCa                                                 <  2:2644625/148‑1 (MQ=255)
                                                                                                 aaGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCa                                                 <  2:1816254/148‑1 (MQ=255)
                                                                                                 aaGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCa                                                 >  1:2673513/1‑148 (MQ=255)
                                                                                                  aGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCAc                                                >  1:1375271/1‑148 (MQ=255)
                                                                                                   gcgcCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGTTATGCGTTGCCCCCATATCCACg                                               >  2:2394134/1‑148 (MQ=255)
                                                                                                        tGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCAGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGc                                          <  2:2935699/148‑1 (MQ=255)
                                                                                                          aGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTa                                        <  1:353911/148‑1 (MQ=255)
                                                                                                          aGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTa                                        >  1:782709/1‑148 (MQ=255)
                                                                                                            ttGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTacg                                      >  2:2922697/1‑148 (MQ=255)
                                                                                                               cTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTacgacg                                   >  2:1552591/1‑148 (MQ=255)
                                                                                                                tCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGt                                  <  2:1995938/148‑1 (MQ=255)
                                                                                                                  gTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTa                                <  2:2608410/148‑1 (MQ=255)
                                                                                                                  gTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTa                                >  2:2396195/1‑148 (MQ=255)
                                                                                                                     aaTCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGc                             <  2:370684/148‑1 (MQ=255)
                                                                                                                     aaTCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCAACTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGc                             >  1:1441069/1‑148 (MQ=255)
                                                                                                                     aaTCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTAGCAGTGTAACGTTTCACCTTATGCTGTCCGCAGACAAAACCCGATAAAACTTGCCGCCATATGCACCCCCGCAACGACCTTAtcc                             >  1:718450/1‑146 (MQ=255)
                                                                                                                      aTCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCt                            >  1:364016/1‑148 (MQ=255)
                                                                                                                       tCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTg                           >  2:646822/1‑148 (MQ=255)
                                                                                                                        cATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGt                          >  1:2352135/1‑148 (MQ=255)
                                                                                                                        cATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGt                          <  2:3035028/148‑1 (MQ=255)
                                                                                                                        cATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGt                          <  2:2683790/148‑1 (MQ=255)
                                                                                                                        cATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGt                          <  1:1021948/148‑1 (MQ=255)
                                                                                                                         aTTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGtt                         <  1:89467/148‑1 (MQ=255)
                                                                                                                             cGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTg                     <  1:2258085/148‑1 (MQ=255)
                                                                                                                               ccGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCa                   <  1:3041066/148‑1 (MQ=255)
                                                                                                                                   tACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAg               >  2:1007491/1‑148 (MQ=255)
                                                                                                                                    aCCCGCCACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAgg              <  2:2097695/148‑1 (MQ=255)
                                                                                                                                    aCCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAgg              <  2:2902204/148‑1 (MQ=255)
                                                                                                                                       cGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTg           >  2:2710750/1‑148 (MQ=255)
                                                                                                                                          cacCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTAc        >  2:2397763/1‑148 (MQ=255)
                                                                                                                                                gCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGACCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGCt  >  2:803139/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
CGCTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGCT  >  CP009273/4297341‑4297632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑