Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,308,395 G→T 11.3% H63N (CAC→AAC)  phnK ← carbon‑phosphorus lyase complex subunit, putative ATP transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,308,3950GT11.3% 663.3 / 39.8 194H63N (CAC→AAC) phnKcarbon‑phosphorus lyase complex subunit, putative ATP transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (83/89);  new base T (12/10);  total (95/99)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 196 total aligned reads displayed.

CCATCAGCCGCTCGCCGATATTGCCGCCTGCCGACACCTGGCGGCGCAGGCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCGGCGCGTAAAG  >  CP009273/4308248‑4308542
                                                                                                                                                   |                                                                                                                                                   
   tCAGCCGCTCGCCGATATTGCCGCCTGCCGACACCTGGCGGCGCAGGCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCTGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAAt                                                                                                                                                  >  2:1006378/1‑148 (MQ=255)
   tCAGCCGCTCGCCGATATTGCCGCCTGCCGACACCTGGCGGCGCAGGCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAAt                                                                                                                                                  >  1:3105125/1‑148 (MQ=255)
   tCAGCCGCTCGCCGATATTGCCGCCTGCCGACACCTGGCGGCGCAGGCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAAt                                                                                                                                                  >  1:1303219/1‑148 (MQ=255)
    cAGCCGCTCGCCGATATTGCCGCCTGCCGACACCTGGCGGCGCAGGCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAAtt                                                                                                                                                 <  1:1717795/148‑1 (MQ=255)
    cAGCCGCTCGCCGATATTGCCGCCTGCCGACACCTGGCGGCGCAGGCCGTCGAGTGGATGATGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTATCGTAGTGAAtt                                                                                                                                                 <  1:3020202/148‑1 (MQ=255)
      gCCGCTCGCCGATATTGCCGCCTGCCGACACCTGGCGGCGCAGGCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTc                                                                                                                                               >  2:2195406/1‑148 (MQ=255)
       ccGCTCGCCGATATTGCCGCCTGCCGACACCTGGCGGCGCAGGCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTcc                                                                                                                                              >  1:806330/1‑148 (MQ=255)
       ccGCTCGCCGATATTGCCGCCTGCCGACACCTGGCGGCGCAGGCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTcc                                                                                                                                              >  1:302983/1‑148 (MQ=255)
         gCTCGCCGATATTGCCGCCTGCCGACACCTGGCGGCGCAGGCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTcccc                                                                                                                                            <  1:1041763/148‑1 (MQ=255)
          cTCGCCGATATTGCCGCCTGCCGACACCTGGCGGCGCAGGCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTccccc                                                                                                                                           >  1:165990/1‑148 (MQ=255)
            cGCCGATATTGCCGCCTGCCGACACCTGGCGGCGCAGGCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCctg                                                                                                                                         >  2:139880/1‑148 (MQ=255)
             gCCGATATTGCCGCCTGCCGACACCTGGCGGCGCAGGCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCctgc                                                                                                                                        >  1:2145131/1‑148 (MQ=255)
                 atatTGCCGCCTGCCGACACCTGGCGGCGCAGGCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTgcg                                                                                                                                    >  2:2738519/1‑148 (MQ=255)
                      gccgccTGCCGACACCTGGCGGCGCAGGCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTc                                                                                                                               <  1:2954610/148‑1 (MQ=255)
                          ccTGCCGACACCTGGCGGCGCAGGCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGgc                                                                                                                           >  1:1154981/1‑148 (MQ=255)
                             gCCGACACCTGGCGGCGCAGGCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGgcgcg                                                                                                                        <  2:2637658/148‑1 (MQ=255)
                                gACACCTGGCGGCGCAGGCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGGAGTTAATTTCCCCCTGCTGCGGCGTCAGGCGCGCgg                                                                                                                     >  2:2228576/1‑148 (MQ=255)
                                  cacCTGGCGGCGCAGGCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGgag                                                                                                                   >  2:1787235/1‑148 (MQ=255)
                                   acCTGGCGGCGCAGGCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGgaga                                                                                                                  <  2:700670/148‑1 (MQ=255)
                                      tGGCGGCGCAGGCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCg                                                                                                               >  2:1614635/1‑148 (MQ=255)
                                      tGGCGGCGCAGGCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCg                                                                                                               >  1:1903135/1‑148 (MQ=255)
                                          ggcgCAGGCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACtt                                                                                                           <  1:2218692/148‑1 (MQ=255)
                                           gcgcAGGCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTc                                                                                                          <  1:3271558/148‑1 (MQ=255)
                                                ggCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTcagcag                                                                                                     <  1:3131580/148‑1 (MQ=255)
                                                ggCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTcagcag                                                                                                     >  1:2751219/1‑148 (MQ=255)
                                                ggCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTcagcag                                                                                                     >  2:840534/1‑148 (MQ=255)
                                                 gCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCagcagc                                                                                                    >  1:1074022/1‑148 (MQ=255)
                                                 gCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCagcagc                                                                                                    >  1:2809679/1‑148 (MQ=255)
                                                  ccGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCg                                                                                                   <  1:2340347/148‑1 (MQ=255)
                                                    gTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTg                                                                                                 <  1:2940823/148‑1 (MQ=255)
                                                    gTCGAGTGGATGCTGATGCACCACGCCCCAGTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTg                                                                                                 <  1:841030/148‑1 (MQ=255)
                                                       gAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTc                                                                                              <  1:3309028/148‑1 (MQ=255)
                                                        aGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTTAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCt                                                                                             >  1:2515486/1‑148 (MQ=255)
                                                          tGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTc                                                                                           <  1:447183/148‑1 (MQ=255)
                                                          tGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTc                                                                                           <  1:2927491/148‑1 (MQ=255)
                                                          tGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTc                                                                                           >  1:505525/1‑148 (MQ=255)
                                                           ggATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTcc                                                                                          >  2:1851405/1‑148 (MQ=255)
                                                           ggATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTcc                                                                                          <  2:3284164/148‑1 (MQ=255)
                                                            gATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTccc                                                                                         >  2:1045770/1‑148 (MQ=255)
                                                            gATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTccc                                                                                         >  1:2384237/1‑148 (MQ=255)
                                                             aTGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTTAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCg                                                                                        >  2:3146667/1‑148 (MQ=255)
                                                                cTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTTAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAg                                                                                     >  2:2732361/1‑148 (MQ=255)
                                                                cTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAg                                                                                     <  2:2863062/148‑1 (MQ=255)
                                                                 tGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGc                                                                                    <  1:527692/148‑1 (MQ=255)
                                                                    tGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCgg                                                                                 >  2:2738433/1‑148 (MQ=255)
                                                                    tGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCgg                                                                                 >  2:99167/1‑148 (MQ=255)
                                                                     gCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGa                                                                                <  1:194806/148‑1 (MQ=255)
                                                                     gCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCACACTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGa                                                                                <  1:3248868/148‑1 (MQ=255)
                                                                      caccacGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGAt                                                                               <  2:2211743/148‑1 (MQ=255)
                                                                         cacGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTcc                                                                            <  2:287860/148‑1 (MQ=255)
                                                                          acGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTccc                                                                           <  1:424899/148‑1 (MQ=255)
                                                                          acGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTccc                                                                           <  1:2090270/148‑1 (MQ=255)
                                                                           cGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTTAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTcccc                                                                          >  2:2209516/1‑148 (MQ=255)
                                                                              cccATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGAc                                                                       >  1:2277616/1‑148 (MQ=255)
                                                                                cATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACaa                                                                     >  2:2401032/1‑148 (MQ=255)
                                                                                cATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACaa                                                                     <  2:2124303/148‑1 (MQ=255)
                                                                                cATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACaa                                                                     >  2:1232665/1‑148 (MQ=255)
                                                                                 aTTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAAt                                                                    >  1:2955327/1‑148 (MQ=255)
                                                                                 aTTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAAt                                                                    >  2:1987648/1‑148 (MQ=255)
                                                                                 aTTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAAt                                                                    >  2:1575779/1‑148 (MQ=255)
                                                                                  ttCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTTAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATg                                                                   <  2:2471989/148‑1 (MQ=255)
                                                                                  ttCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTTAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATg                                                                   <  2:1888048/148‑1 (MQ=255)
                                                                                  ttCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTTAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATg                                                                   <  2:2593907/148‑1 (MQ=255)
                                                                                   tCGTTACGCTGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTCGTGAATTTCCCCCTGGTGCGGCGTCAGGCGCGCGGAGATCGACTTAAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACTATGc                                                                  <  1:255861/148‑1 (MQ=255)
                                                                                    cGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGcc                                                                 <  2:2569610/148‑1 (MQ=255)
                                                                                         cGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTTAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCa                                                            <  1:301552/148‑1 (MQ=255)
                                                                                             gcagGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTc                                                        >  1:1137629/1‑148 (MQ=255)
                                                                                               agGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTTAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTccc                                                      <  1:1168673/148‑1 (MQ=255)
                                                                                                     cggcggTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGcc                                                <  2:1561883/148‑1 (MQ=255)
                                                                                                      ggcggTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCa                                               <  2:60624/148‑1 (MQ=255)
                                                                                                         ggTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATaa                                            <  1:1768656/148‑1 (MQ=255)
                                                                                                         ggTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATaa                                            <  1:3186931/148‑1 (MQ=255)
                                                                                                           tCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAAt                                          <  2:527234/148‑1 (MQ=255)
                                                                                                           tCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAAt                                          >  1:1373805/1‑148 (MQ=255)
                                                                                                           tCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAAt                                          >  1:2167872/1‑148 (MQ=255)
                                                                                                           tCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAAt                                          >  1:2032707/1‑148 (MQ=255)
                                                                                                            cGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTTAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATc                                         >  2:2293781/1‑148 (MQ=255)
                                                                                                            cGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATc                                         <  2:2950518/148‑1 (MQ=255)
                                                                                                                 tCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAag                                    >  1:2228136/1‑148 (MQ=255)
                                                                                                                  cGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAaga                                   <  1:2987377/148‑1 (MQ=255)
                                                                                                                      cATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACa                               <  1:1851405/148‑1 (MQ=255)
                                                                                                                      cATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACa                               <  1:835647/148‑1 (MQ=255)
                                                                                                                         tGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCg                            >  1:2239895/1‑148 (MQ=255)
                                                                                                                         tGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCg                            >  1:944595/1‑148 (MQ=255)
                                                                                                                          gCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGc                           <  1:2211497/148‑1 (MQ=255)
                                                                                                                           cATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCt                          >  1:626867/1‑148 (MQ=255)
                                                                                                                              aCAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTaaa                       <  1:103852/148‑1 (MQ=255)
                                                                                                                                aGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGc                     <  1:1553001/148‑1 (MQ=255)
                                                                                                                                  cGAACGGTTTTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCt                   <  1:2469054/148‑1 (MQ=255)
                                                                                                                                         ttCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCg            >  2:2033378/1‑148 (MQ=255)
                                                                                                                                         ttCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCg            >  2:2033250/1‑148 (MQ=255)
                                                                                                                                         ttCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCg            >  2:1365870/1‑148 (MQ=255)
                                                                                                                                          tctcGTAGTTAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCgg           >  1:801880/1‑148 (MQ=255)
                                                                                                                                          tctcGTAGTTAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCgg           >  1:333871/1‑148 (MQ=255)
                                                                                                                                             cGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCGgcgc        >  1:474289/1‑148 (MQ=255)
                                                                                                                                               tAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCGGCGCGt      >  2:478310/1‑148 (MQ=255)
                                                                                                                                                 gTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCGGCGCGTaa    >  1:285740/1‑148 (MQ=255)
                                                                                                                                                  tGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCGGCGCGTaaa   <  1:2195406/148‑1 (MQ=255)
                                                                                                                                                  tGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCGGCGCGTaaa   >  2:731729/1‑148 (MQ=255)
                                                                                                                                                  tGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCGGCGCGTaaa   >  2:926094/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCATCAGCCGCTCGCCGATATTGCCGCCTGCCGACACCTGGCGGCGCAGGCCGTCGAGTGGATGCTGATGCACCACGCCCCATTCGGTACGCAGCAGGCGACGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCGGCGCGTAAAG  >  CP009273/4308248‑4308542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑