Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,318,517 A→T 90.4% T522T (ACA→ACT)  crfC → clamp‑binding sister replication fork colocalization protein, dynamin‑related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,318,5170AT90.4% 529.0 / 35.1 176T522T (ACA→ACT) crfCclamp‑binding sister replication fork colocalization protein, dynamin‑related
Reads supporting (aligned to +/- strand):  ref base A (9/8);  new base T (64/95);  total (73/103)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 177 total aligned reads displayed.

GCGAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTT  >  CP009273/4318372‑4318660
                                                                                                                                                 |                                                                                                                                               
gcgAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGActct                                                                                                                                               >  2:2161691/1‑148 (MQ=255)
   agaTTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGAATCTGtt                                                                                                                                            >  1:1003046/1‑148 (MQ=255)
   agaTTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCGTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGtt                                                                                                                                            >  2:2931652/1‑148 (MQ=255)
    gaTTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTa                                                                                                                                           >  1:1743425/1‑148 (MQ=255)
       tAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAg                                                                                                                                        <  1:1636035/148‑1 (MQ=255)
             tGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGgcg                                                                                                                                  >  2:143612/1‑148 (MQ=255)
                aaTCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAg                                                                                                                               <  1:935295/148‑1 (MQ=255)
                   cGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAac                                                                                                                            >  2:1675348/1‑148 (MQ=255)
                    gAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAaca                                                                                                                           <  1:58592/148‑1 (MQ=255)
                    gAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAaca                                                                                                                           <  1:2057482/148‑1 (MQ=255)
                       cTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCa                                                                                                                        >  2:1858727/1‑148 (MQ=255)
                       cTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCa                                                                                                                        <  1:1883687/148‑1 (MQ=255)
                        tGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCAt                                                                                                                       <  1:2974866/148‑1 (MQ=255)
                           cccTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAg                                                                                                                    >  2:1508833/1‑148 (MQ=255)
                             cTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTc                                                                                                                  >  1:1951894/1‑148 (MQ=255)
                             cTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTc                                                                                                                  >  1:2502048/1‑148 (MQ=255)
                             cTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTc                                                                                                                  >  1:1132551/1‑148 (MQ=255)
                                aCCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTcgcg                                                                                                               <  1:154279/148‑1 (MQ=255)
                                aCCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTcgcg                                                                                                               >  1:836509/1‑148 (MQ=255)
                                aCCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTcgcg                                                                                                               >  2:2961151/1‑148 (MQ=255)
                                aCCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTcgcg                                                                                                               >  1:836662/1‑148 (MQ=255)
                                aCCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCACTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTcgcg                                                                                                               >  1:1724993/1‑148 (MQ=255)
                                 ccTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGa                                                                                                              >  1:1020834/1‑148 (MQ=255)
                                     cGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACttt                                                                                                          <  1:2941881/148‑1 (MQ=255)
                                       ccAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTAc                                                                                                        >  1:2475429/1‑148 (MQ=255)
                                       ccAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTAc                                                                                                        >  1:682804/1‑148 (MQ=255)
                                       ccAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTAc                                                                                                        >  1:682813/1‑148 (MQ=255)
                                        cAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACg                                                                                                       >  1:1504206/1‑148 (MQ=255)
                                        cAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACg                                                                                                       <  2:131945/148‑1 (MQ=255)
                                             aCTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCg                                                                                                  >  1:3016967/1‑148 (MQ=255)
                                              cTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGt                                                                                                 >  1:2163768/1‑148 (MQ=255)
                                               tttCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGtt                                                                                                <  2:2411345/148‑1 (MQ=255)
                                                     cGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCg                                                                                          <  2:316533/148‑1 (MQ=255)
                                                     cGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCg                                                                                          <  2:406146/148‑1 (MQ=255)
                                                        cAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGc                                                                                       >  1:1063670/1‑148 (MQ=255)
                                                         aaCAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCt                                                                                      <  1:984395/148‑1 (MQ=255)
                                                         aaCAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCt                                                                                      >  2:2242798/1‑148 (MQ=255)
                                                              cAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAAt                                                                                 >  2:1098514/1‑148 (MQ=255)
                                                                 gATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCa                                                                              >  1:1410430/1‑148 (MQ=255)
                                                                 gATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCa                                                                              >  2:2635856/1‑148 (MQ=255)
                                                                  aTGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCAc                                                                             >  1:1461585/1‑148 (MQ=255)
                                                                    gcgcTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCAccc                                                                           <  2:2240571/148‑1 (MQ=255)
                                                                     cgcTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCt                                                                          <  2:315925/148‑1 (MQ=255)
                                                                     cgcTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCt                                                                          <  2:1102074/148‑1 (MQ=255)
                                                                      gcTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGATCTTGAATCCACCCtt                                                                         <  1:2290773/148‑1 (MQ=255)
                                                                           aGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCg                                                                    <  2:3262812/148‑1 (MQ=255)
                                                                              tGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGgt                                                                 <  1:903085/148‑1 (MQ=255)
                                                                                cAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGgtgt                                                               <  2:3240129/148‑1 (MQ=255)
                                                                                cAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGgtgt                                                               <  1:2673309/148‑1 (MQ=255)
                                                                                   ttAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTaa                                                            >  1:3163030/1‑148 (MQ=255)
                                                                                      gccgccTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCg                                                         <  2:472385/148‑1 (MQ=255)
                                                                                       cctccTTGTTTCAGGATGTTTCTGAGCCGTTAAGGGAGATTCGTACCCGCTGTGTGACTCTGTTACAGACGGCGCAGAACACCATCAGTAGCGACTTTACGCTGCGTTTTGCCGAGATTGATTACACCCTTTCCCGGGTGTTAACCGa                                                        <  2:1692085/145‑1 (MQ=255)
                                                                                       ccgccTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGa                                                        <  2:2346153/148‑1 (MQ=255)
                                                                                       ccgccTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGa                                                        <  1:1508384/148‑1 (MQ=255)
                                                                                       ccgccTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGa                                                        <  2:1684636/148‑1 (MQ=255)
                                                                                       ccgccTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGa                                                        <  2:321388/148‑1 (MQ=255)
                                                                                       ccgccTTGTGTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGa                                                        <  2:686268/148‑1 (MQ=255)
                                                                                        cgccTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGAt                                                       <  2:2078002/148‑1 (MQ=255)
                                                                                          ccTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGt                                                     >  2:840559/1‑148 (MQ=255)
                                                                                           cTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGtt                                                    <  2:1282521/148‑1 (MQ=255)
                                                                                            ttGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTa                                                   <  2:581098/148‑1 (MQ=255)
                                                                                              gcttCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTAtt                                                 <  2:2049689/146‑1 (MQ=255)
                                                                                               tttCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTc                                                >  2:2470744/1‑148 (MQ=255)
                                                                                               tttCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTc                                                >  2:2881196/1‑148 (MQ=255)
                                                                                               tttCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTc                                                >  2:812387/1‑148 (MQ=255)
                                                                                                   aGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCNGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGccc                                            <  1:1681264/148‑1 (MQ=255)
                                                                                                   aGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGccc                                            <  1:1924350/148‑1 (MQ=255)
                                                                                                        gatTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTg                                       <  1:2567836/148‑1 (MQ=255)
                                                                                                          ttCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAg                                     <  2:2084863/148‑1 (MQ=255)
                                                                                                          ttCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAg                                     <  2:347849/148‑1 (MQ=255)
                                                                                                          ttCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAg                                     <  2:347916/148‑1 (MQ=255)
                                                                                                          ttCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAg                                     <  2:1951894/148‑1 (MQ=255)
                                                                                                           tCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGc                                    <  1:2904887/148‑1 (MQ=255)
                                                                                                           tCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGc                                    >  2:2681657/1‑148 (MQ=255)
                                                                                                           tCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGc                                    <  2:1417883/148‑1 (MQ=255)
                                                                                                           tCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGc                                    >  1:3288042/1‑148 (MQ=255)
                                                                                                             ggAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGcaa                                  >  2:1934526/1‑148 (MQ=255)
                                                                                                              gAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGcaac                                 >  1:495145/1‑148 (MQ=255)
                                                                                                               aGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGcaaca                                <  1:1066583/148‑1 (MQ=255)
                                                                                                               aGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATACACCCTTTGCCGGGTGTTAAACGATGTTATTCGCCCCAATGAGcaaaa                                >  1:1248984/1‑146 (MQ=255)
                                                                                                                gCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGcaacaa                               >  1:2753682/1‑148 (MQ=255)
                                                                                                                  cGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGt                             <  2:1989665/148‑1 (MQ=255)
                                                                                                                  cGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGt                             <  2:2498855/148‑1 (MQ=255)
                                                                                                                    ttAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCa                           >  1:2143060/1‑148 (MQ=255)
                                                                                                                      aaGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCaaa                         <  1:3241287/148‑1 (MQ=255)
                                                                                                                       aGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCaaaa                        <  1:2369378/148‑1 (MQ=255)
                                                                                                                        gCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAAt                       >  2:2663407/1‑148 (MQ=255)
                                                                                                                           agaTTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGa                    <  2:3301307/148‑1 (MQ=255)
                                                                                                                            gaTTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGaa                   <  1:2331764/148‑1 (MQ=255)
                                                                                                                            gaTTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGaa                   <  2:90049/148‑1 (MQ=255)
                                                                                                                             aTTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAAt                  <  1:1969313/148‑1 (MQ=255)
                                                                                                                             aTTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAAt                  <  1:92603/148‑1 (MQ=255)
                                                                                                                             aTTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAAt                  <  1:2979794/148‑1 (MQ=255)
                                                                                                                              ttCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAAtt                 >  2:1145959/1‑148 (MQ=255)
                                                                                                                               tCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTg                <  2:2597850/148‑1 (MQ=255)
                                                                                                                                    cccGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGa           >  1:1833945/1‑148 (MQ=255)
                                                                                                                                    cccGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGa           <  2:2118317/148‑1 (MQ=255)
                                                                                                                                         tgtgAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAg      <  1:444053/148‑1 (MQ=255)
                                                                                                                                           tgAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAggg    >  1:3200483/1‑148 (MQ=255)
                                                                                                                                            gagaCTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGt   <  2:3302689/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GCGAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTT  >  CP009273/4318372‑4318660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑