Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,337,551 C→A 5.7% V337V (GTG→GTT)  dcuB ← C4‑dicarboxylate transporter, anaerobic; DcuS co‑sensor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,337,5510CA 5.7% 731.8 / 12.6 192V337V (GTG→GTT) dcuBC4‑dicarboxylate transporter, anaerobic; DcuS co‑sensor
Reads supporting (aligned to +/- strand):  ref base C (77/104);  new base A (3/8);  total (80/112)
Fisher's exact test for biased strand distribution p-value = 3.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 194 total aligned reads displayed.

GCAAGCCGGTGCTGAAGCCACGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGACGCGGGATTGGTTTT  >  CP009273/4337404‑4337697
                                                                                                                                                   |                                                                                                                                                  
gCAAGCCGGTGCTGAAGCCACGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTc                                                                                                                                                    >  1:3151255/1‑148 (MQ=255)
    gCCGGTGCTGAAGCCACGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCa                                                                                                                                                <  2:2198376/148‑1 (MQ=255)
    gCCGGTGCTGAAGCCACGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCa                                                                                                                                                <  1:1847479/148‑1 (MQ=255)
    gCCGGTGCTGAAGCCACGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCa                                                                                                                                                >  2:1906043/1‑148 (MQ=255)
    gCCGGTGCTGAAGCCACGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCa                                                                                                                                                <  2:1123412/148‑1 (MQ=255)
    gCCGGTGCTGAAGCCACGATATATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCa                                                                                                                                                >  1:2239091/1‑148 (MQ=255)
      cGGTGCTGAAGCCACGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCAtt                                                                                                                                              <  1:2066353/148‑1 (MQ=255)
               aGCCACGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGt                                                                                                                                     >  1:2700913/1‑148 (MQ=255)
               aGCCACGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGt                                                                                                                                     >  2:233359/1‑148 (MQ=255)
                   aCGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGc                                                                                                                                 <  2:1257131/148‑1 (MQ=255)
                   aCGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGc                                                                                                                                 >  1:1141104/1‑148 (MQ=255)
                    cGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGcc                                                                                                                                >  1:761798/1‑148 (MQ=255)
                    cGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGcc                                                                                                                                <  1:1790341/148‑1 (MQ=255)
                    cGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGcc                                                                                                                                <  1:1984111/148‑1 (MQ=255)
                     gATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGccc                                                                                                                               >  1:1526011/1‑148 (MQ=255)
                     gATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGccc                                                                                                                               >  1:269590/1‑148 (MQ=255)
                        gTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGa                                                                                                                            >  1:441669/1‑148 (MQ=255)
                         tATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGaa                                                                                                                           >  1:2920620/1‑148 (MQ=255)
                          aTGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAAt                                                                                                                          <  2:2653946/148‑1 (MQ=255)
                             ccGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTc                                                                                                                       <  2:3150459/148‑1 (MQ=255)
                                gATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGa                                                                                                                    <  1:1210621/148‑1 (MQ=255)
                                 aTCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGAc                                                                                                                   <  1:1076435/148‑1 (MQ=255)
                                 aTCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGAc                                                                                                                   <  1:957966/148‑1 (MQ=255)
                                 aTCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGAc                                                                                                                   <  1:1366784/148‑1 (MQ=255)
                                  tCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACa                                                                                                                  <  2:2300367/148‑1 (MQ=255)
                                      cGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATg                                                                                                              >  1:340734/1‑148 (MQ=255)
                                          gATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCa                                                                                                          >  2:2385429/1‑148 (MQ=255)
                                               ccAGCTCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGaa                                                                                                     >  2:309926/1‑148 (MQ=255)
                                                cAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAAc                                                                                                    >  2:623699/1‑148 (MQ=255)
                                                    gcgACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATgg                                                                                                <  1:982304/148‑1 (MQ=255)
                                                     cgACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGt                                                                                               <  2:2613800/148‑1 (MQ=255)
                                                       tccGGATCATTTGCCTCCAGCGGCGATGCCTGATAGTTTACAAAGTTGGAAACCAGCAGCAGAATTATGGCATTGGCCCTGGGATTCTCTTTCAACATTTCTGGCTGTAGGCCCTGTGTTTGAGTGATTTGCTGACCGAACATGGttt                                                                                             <  2:533556/147‑1 (MQ=255)
                                                       aCCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGttt                                                                                             <  2:306310/148‑1 (MQ=255)
                                                       aCCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGttt                                                                                             >  1:1415901/1‑148 (MQ=255)
                                                       aCCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGttt                                                                                             <  2:745512/148‑1 (MQ=255)
                                                        ccGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTc                                                                                            >  1:282773/1‑148 (MQ=255)
                                                        ccGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTc                                                                                            <  2:1598918/148‑1 (MQ=255)
                                                          ggAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTg                                                                                          <  2:2700913/148‑1 (MQ=255)
                                                           gAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGc                                                                                         >  2:1868384/1‑148 (MQ=255)
                                                            aaCAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCCGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGcc                                                                                        >  2:1753180/1‑148 (MQ=255)
                                                              cAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGccat                                                                                      >  1:3111304/1‑148 (MQ=255)
                                                              cAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGccat                                                                                      >  1:2147543/1‑148 (MQ=255)
                                                                 tCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGccatcca                                                                                   <  2:2686006/148‑1 (MQ=255)
                                                                 tCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGccatcca                                                                                   <  2:2007772/148‑1 (MQ=255)
                                                                 tCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGccatcca                                                                                   >  1:64972/1‑148 (MQ=255)
                                                                       ccAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGAt                                                                             <  1:2996219/148‑1 (MQ=255)
                                                                          gcgcCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATAcc                                                                          >  2:1466181/1‑148 (MQ=255)
                                                                              cgcAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTac                                                                      <  1:3307045/148‑1 (MQ=255)
                                                                              cgcAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTac                                                                      <  2:1945315/148‑1 (MQ=255)
                                                                                      gagaGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCAc                                                              >  1:595204/1‑148 (MQ=255)
                                                                                        gagTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGa                                                            >  2:1700019/1‑148 (MQ=255)
                                                                                         agTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGAt                                                           >  1:1321623/1‑148 (MQ=255)
                                                                                          gTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATg                                                          <  2:3009336/148‑1 (MQ=255)
                                                                                          gTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATg                                                          <  2:1362345/148‑1 (MQ=255)
                                                                                           tttACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATgg                                                         <  1:3289593/148‑1 (MQ=255)
                                                                                              aCAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGa                                                      <  2:1209926/148‑1 (MQ=255)
                                                                                                aaaCTTGGAAACCAGCAGCAGAACACTGGCATAGGCACACGGATACTCTTTCACCATTTCACCCAGTACGACATGTATTTCAGACTTATGCGCACCGAACATGGTTTATGCCATCCATGCGATACCGTACACCGCAACGATGGCGATc                                                    <  2:459406/148‑1 (MQ=255)
                                                                                                  aCTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCAt                                                  >  1:2582207/1‑148 (MQ=255)
                                                                                                  aCTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCAt                                                  <  1:241745/148‑1 (MQ=255)
                                                                                                   cTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATa                                                 >  2:1141291/1‑148 (MQ=255)
                                                                                                    ttGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATAc                                                <  1:233359/148‑1 (MQ=255)
                                                                                                    ttGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATAc                                                >  2:2152136/1‑148 (MQ=255)
                                                                                                        aaaCCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACcgga                                            <  2:2239065/148‑1 (MQ=255)
                                                                                                         aaCCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACcggaa                                           <  1:1203059/148‑1 (MQ=255)
                                                                                                             agcagcagAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACcggaacgga                                       <  2:990622/148‑1 (MQ=255)
                                                                                                             agcagcagAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACcggaacgga                                       <  2:990584/148‑1 (MQ=255)
                                                                                                                  cagAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACt                                  >  2:857644/1‑148 (MQ=255)
                                                                                                                   agAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACtt                                 <  2:2632054/148‑1 (MQ=255)
                                                                                                                     aaCAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCg                               <  2:2617706/148‑1 (MQ=255)
                                                                                                                      aCAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGt                              <  2:3230910/148‑1 (MQ=255)
                                                                                                                      aCAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGt                              <  2:483553/148‑1 (MQ=255)
                                                                                                                      aCAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGt                              >  1:2092248/1‑148 (MQ=255)
                                                                                                                       cAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGtt                             >  1:3194421/1‑148 (MQ=255)
                                                                                                                       cAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGtt                             >  1:2086169/1‑148 (MQ=255)
                                                                                                                       cAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGtt                             <  2:2672407/148‑1 (MQ=255)
                                                                                                                        aaTGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGttt                            <  2:1208895/148‑1 (MQ=255)
                                                                                                                         aTGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGtttt                           <  1:538554/148‑1 (MQ=255)
                                                                                                                          tGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGACGACTTCGttttt                          >  2:2511725/1‑148 (MQ=255)
                                                                                                                          tGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGttttt                          >  2:2510389/1‑148 (MQ=255)
                                                                                                                          tGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGttttt                          <  1:309926/148‑1 (MQ=255)
                                                                                                                           ggCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGtttttt                         >  2:2916505/1‑148 (MQ=255)
                                                                                                                           ggCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGtttttt                         >  2:1321959/1‑148 (MQ=255)
                                                                                                                            gCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTg                        <  1:1997117/148‑1 (MQ=255)
                                                                                                                               tAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTgaga                     >  2:987695/1‑148 (MQ=255)
                                                                                                                                aGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGAt                    >  2:412210/1‑148 (MQ=255)
                                                                                                                                 ggCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATa                   >  2:357219/1‑148 (MQ=255)
                                                                                                                                 ggCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATa                   <  1:2729823/148‑1 (MQ=255)
                                                                                                                                 ggCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATa                   <  1:1078259/148‑1 (MQ=255)
                                                                                                                                     cACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGAcg               >  2:3036226/1‑148 (MQ=255)
                                                                                                                                     cACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGAcg               <  2:441665/148‑1 (MQ=255)
                                                                                                                                     cACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGAcg               <  1:725202/148‑1 (MQ=255)
                                                                                                                                     cACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGAcg               <  1:2385429/148‑1 (MQ=255)
                                                                                                                                         gATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGACGCggg           >  1:1312483/1‑148 (MQ=255)
                                                                                                                                          aTACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGACGCGGGa          <  1:2592283/148‑1 (MQ=255)
                                                                                                                                          aTACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGACGCGGGa          <  1:1713521/148‑1 (MQ=255)
                                                                                                                                            aCTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGACGCGGGAtt        <  2:2821839/148‑1 (MQ=255)
                                                                                                                                            aCTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGACGCGGGAtt        <  2:694536/148‑1 (MQ=255)
                                                                                                                                             ctctTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGACGCGGGATTg       <  1:1881743/148‑1 (MQ=255)
                                                                                                                                              tctTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGACGCGGGATTgg      <  1:2944149/148‑1 (MQ=255)
                                                                                                                                                 ttCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGACGCGGGATTGGttt   >  2:2706818/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCAAGCCGGTGCTGAAGCCACGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGACGCGGGATTGGTTTT  >  CP009273/4337404‑4337697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑