Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,350,223 G→A 91.2% A510V (GCT→GTT)  cadC ← cadBA operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,350,2230GA91.2% 761.1 / 51.8 250A510V (GCT→GTT) cadCcadBA operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (10/12);  new base A (104/124);  total (114/136)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 250 total aligned reads displayed.

TTGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTC  >  CP009273/4350078‑4350366
                                                                                                                                                 |                                                                                                                                               
 tGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACaa                                                                                                                                              >  2:1875561/1‑148 (MQ=255)
  gCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAg                                                                                                                                             <  1:1463855/148‑1 (MQ=255)
     tGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGaaa                                                                                                                                          >  1:1644899/1‑148 (MQ=255)
      gACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAAt                                                                                                                                         >  1:510116/1‑148 (MQ=255)
      gACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAAt                                                                                                                                         >  1:215287/1‑148 (MQ=255)
        cATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAAttt                                                                                                                                       <  2:596961/148‑1 (MQ=255)
        cATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAAttt                                                                                                                                       >  1:1890744/1‑148 (MQ=255)
         aTTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTg                                                                                                                                      <  1:67450/148‑1 (MQ=255)
         aTTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTg                                                                                                                                      <  2:2677394/148‑1 (MQ=255)
                 gTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATa                                                                                                                              <  2:2611175/148‑1 (MQ=255)
                 gTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATa                                                                                                                              >  1:2528596/1‑148 (MQ=255)
                 gTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATa                                                                                                                              >  1:874406/1‑148 (MQ=255)
                  ttAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATaa                                                                                                                             <  1:1446220/148‑1 (MQ=255)
                   tAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAg                                                                                                                            <  2:2036591/148‑1 (MQ=255)
                    aaaTTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAgg                                                                                                                           <  1:2062075/148‑1 (MQ=255)
                      aTTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTa                                                                                                                         >  1:327164/1‑148 (MQ=255)
                      aTTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTGGTCGAGATAAGGTa                                                                                                                         >  2:1134958/1‑148 (MQ=255)
                       tttATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTac                                                                                                                        >  1:1319181/1‑148 (MQ=255)
                              aataaAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATaa                                                                                                                 >  2:3203289/1‑148 (MQ=255)
                                taaAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAgg                                                                                                               >  2:787101/1‑148 (MQ=255)
                                 aaaaaTTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGa                                                                                                              <  1:804862/148‑1 (MQ=255)
                                 aaaaaTTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGa                                                                                                              >  2:2175364/1‑148 (MQ=255)
                                  aaaaTTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGaa                                                                                                             <  1:3204354/148‑1 (MQ=255)
                                   aaaTTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAAc                                                                                                            <  2:3085367/148‑1 (MQ=255)
                                    aaTTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACa                                                                                                           >  1:1154925/1‑148 (MQ=255)
                                     aTTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAg                                                                                                          >  1:395114/1‑148 (MQ=255)
                                       tATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGaa                                                                                                        <  2:460408/148‑1 (MQ=255)
                                         tGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGt                                                                                                      <  1:692427/148‑1 (MQ=255)
                                         tGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGt                                                                                                      <  2:1520712/148‑1 (MQ=255)
                                              cAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGa                                                                                                 >  2:1077496/1‑148 (MQ=255)
                                                aataaatTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAat                                                                                               >  1:2226881/1‑148 (MQ=255)
                                                 ataaatTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAata                                                                                              >  2:2229642/1‑148 (MQ=255)
                                                 ataaatTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAata                                                                                              >  2:2446164/1‑148 (MQ=255)
                                                 ataaatTGCCGCAACATATTAGACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAata                                                                                              <  2:2763021/148‑1 (MQ=255)
                                                  taaatTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAatat                                                                                             >  2:1859928/1‑148 (MQ=255)
                                                   aaatTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAAtata                                                                                            <  2:1714619/148‑1 (MQ=255)
                                                     atTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATAcc                                                                                          <  1:545101/148‑1 (MQ=255)
                                                      ttGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCa                                                                                         <  1:175183/148‑1 (MQ=255)
                                                       tGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCAt                                                                                        <  1:1874997/148‑1 (MQ=255)
                                                         ccGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCAttt                                                                                      <  1:2346543/148‑1 (MQ=255)
                                                         ccGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCAttt                                                                                      <  2:1415916/148‑1 (MQ=255)
                                                         ccGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCAttt                                                                                      <  1:2900408/148‑1 (MQ=255)
                                                            cAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCa                                                                                   <  2:1511700/148‑1 (MQ=255)
                                                             aaCATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCaa                                                                                  >  2:3177711/1‑148 (MQ=255)
                                                              aCATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAAt                                                                                 >  2:2481984/1‑148 (MQ=255)
                                                              aCATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAAt                                                                                 >  1:1895663/1‑148 (MQ=255)
                                                                 tattatACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCa                                                                              <  2:1906156/148‑1 (MQ=255)
                                                                  attatACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAg                                                                             >  2:2318078/1‑148 (MQ=255)
                                                                  attatACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAg                                                                             <  1:2965099/148‑1 (MQ=255)
                                                                       aCCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAg                                                                        <  1:1706193/148‑1 (MQ=255)
                                                                          aaCAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTTATTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAATGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGgt                                                                     <  1:2420566/148‑1 (MQ=255)
                                                                           aCAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGgtg                                                                    >  2:1972202/1‑148 (MQ=255)
                                                                               gAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTg                                                                <  1:107363/148‑1 (MQ=255)
                                                                               gAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTg                                                                <  1:207802/148‑1 (MQ=255)
                                                                                  cATACAAAAACTCAACAACAACTATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGccc                                                             <  2:1786178/148‑1 (MQ=255)
                                                                                  cATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGccc                                                             >  2:1678398/1‑148 (MQ=255)
                                                                                    tACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCt                                                           <  2:1605510/148‑1 (MQ=255)
                                                                                     aCAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCGAGTAAAGGGTGTTTGCCCCTg                                                          >  2:3252726/1‑148 (MQ=255)
                                                                                     aCAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTg                                                          <  1:1057495/148‑1 (MQ=255)
                                                                                      cAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTgg                                                         >  2:1016921/1‑148 (MQ=255)
                                                                                          aaCTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGt                                                     <  1:2481984/148‑1 (MQ=255)
                                                                                            cTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTaa                                                   >  2:591261/1‑148 (MQ=255)
                                                                                            cTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTaa                                                   <  1:3120803/148‑1 (MQ=255)
                                                                                            cTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTaa                                                   <  1:2127546/148‑1 (MQ=255)
                                                                                              caacaacaaATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAAt                                                 <  2:482941/148‑1 (MQ=255)
                                                                                                  aacaaATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTaaa                                             <  2:2957782/148‑1 (MQ=255)
                                                                                                   acaaATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAg                                            >  1:1616014/1‑148 (MQ=255)
                                                                                                    caaATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAgg                                           >  2:520043/1‑148 (MQ=255)
                                                                                                    caaATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAgg                                           <  1:1359332/148‑1 (MQ=255)
                                                                                                     aaaTATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGc                                          <  1:3146744/148‑1 (MQ=255)
                                                                                                      aaTATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCg                                         <  1:1200630/148‑1 (MQ=255)
                                                                                                       atatTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCgg                                        >  2:2154739/1‑148 (MQ=255)
                                                                                                        tatTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTCCAACATAAGGAACAGAAGTCTTGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCACTGGGCGTAAATTAAATGCGGt                                       >  2:266695/1‑148 (MQ=255)
                                                                                                        tatTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGt                                       >  1:657424/1‑148 (MQ=255)
                                                                                                         atTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTg                                      >  1:2620717/1‑148 (MQ=255)
                                                                                                              cGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGata                                 <  1:589046/148‑1 (MQ=255)
                                                                                                                 gCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGc                              <  2:874406/148‑1 (MQ=255)
                                                                                                                  cATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAATGGCGGTGAGATATGCa                             >  2:2079502/1‑148 (MQ=255)
                                                                                                                    tAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATACGGTACAACATAAGGAACAGAAGTCTGGAATATACCAGTTTCAATCCAGTAACGGGTGTTTGCCCCTGGGCGTCAATTAAAGGCGGGGAGATATGCATc                           >  2:2031347/1‑148 (MQ=255)
                                                                                                                    tAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATc                           <  2:819883/148‑1 (MQ=255)
                                                                                                                       aTCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAgc                        <  1:2678968/148‑1 (MQ=255)
                                                                                                                       aTCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAgc                        <  2:291432/148‑1 (MQ=255)
                                                                                                                       aTCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAgc                        <  1:1222324/148‑1 (MQ=255)
                                                                                                                             ccGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTc                  >  1:1849645/1‑148 (MQ=255)
                                                                                                                             ccGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTc                  <  2:1607731/148‑1 (MQ=255)
                                                                                                                             ccGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTc                  >  1:2653980/1‑148 (MQ=255)
                                                                                                                             ccGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTc                  <  2:3002701/148‑1 (MQ=255)
                                                                                                                              cGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTcc                 <  1:2874810/148‑1 (MQ=255)
                                                                                                                                 aGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCgg              <  1:1130594/148‑1 (MQ=255)
                                                                                                                                 aGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCgg              <  1:2829276/148‑1 (MQ=255)
                                                                                                                                  gTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGt             >  2:561188/1‑148 (MQ=255)
                                                                                                                                     aCTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCa          >  1:1979301/1‑148 (MQ=255)
                                                                                                                                      cTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCAt         >  1:2045320/1‑148 (MQ=255)
                                                                                                                                       ttATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATc        >  2:1271484/1‑148 (MQ=255)
                                                                                                                                          ttCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATcccc     <  1:1460905/148‑1 (MQ=255)
                                                                                                                                          ttCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATcccc     <  2:3307892/148‑1 (MQ=255)
                                                                                                                                          ttCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATcccc     >  1:1116727/1‑148 (MQ=255)
                                                                                                                                          ttCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATcccc     <  1:413719/148‑1 (MQ=255)
                                                                                                                                           tCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCt    <  2:2601716/148‑1 (MQ=255)
                                                                                                                                             tGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCttc  <  2:725586/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TTGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTC  >  CP009273/4350078‑4350366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑