BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,357,772	G→T	5.1%	Q125K (CAA→AAA)	aspA ←	aspartate ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,357,772	0	G	T	5.1%	748.3 / 2.6	222	Q125K (CAA→AAA)	aspA	aspartate ammonia‑lyase
Reads supporting (aligned to +/- strand): ref base G (100/105); new base T (15/2); total (115/107)
Fisher's exact test for biased strand distribution p-value = 1.79e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 230 total aligned reads displayed.

AGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCATTTTCCGTTGTTCAGGACTTCATCACATGCGGCAATGAT  >  CP009273/4357625‑4357919

aGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTg                                                                                                                                                     <  1:2741677/148‑1 (MQ=255)

aGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCATTTTg                                                                                                                                                     >  2:1475340/1‑148 (MQ=255)

  ccTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACCTTTGTTAACATGGTCGTTAGGGTTAAGTTACTTATATTACCCATTTTgtt                                                                                                                                                   >  1:407781/1‑146 (MQ=255)

   cTTCACGCAGTTGGTTAATAGCATCTTCAAGCTTAATCAGTGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTTTGCTTCGTTAGTGGACTGACATGTGTTAAGAGGGTCGTTCGGGTTCTGGTACTGATATTCACCTTTTTGGTg                                                                                                                                                  <  1:2138648/148‑1 (MQ=255)

    ttCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGa                                                                                                                                                 >  2:930504/1‑148 (MQ=255)

          cAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATc                                                                                                                                           <  2:2778682/148‑1 (MQ=255)

             ttGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGt                                                                                                                                        >  2:2739013/1‑148 (MQ=255)

             ttGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGt                                                                                                                                        >  1:2555120/1‑148 (MQ=255)

              tGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTGCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCCGtt                                                                                                                                       >  1:82359/1‑148 (MQ=255)

                 ttAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCa                                                                                                                                    >  2:1235819/1‑148 (MQ=255)

                  tAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAg                                                                                                                                   <  1:1316441/148‑1 (MQ=255)

                  tAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAg                                                                                                                                   <  1:1316170/148‑1 (MQ=255)

                  tAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAg                                                                                                                                   >  2:248258/1‑148 (MQ=255)

                   aaTCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGa                                                                                                                                  <  2:1863120/148‑1 (MQ=255)

                   aaTCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCGTTTTGGTGACCCATCAGTTCCAGa                                                                                                                                  >  1:972142/1‑148 (MQ=255)

                          tCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGatat                                                                                                                           <  1:2376018/148‑1 (MQ=255)

                          tCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTAACCTTTTTGGTGACCCATCAGTTCCAGACCGatat                                                                                                                           >  1:2878336/1‑148 (MQ=255)

                           cTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATAtt                                                                                                                          <  1:767233/148‑1 (MQ=255)

                           cTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATAtt                                                                                                                          <  1:2227432/148‑1 (MQ=255)

                            tACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTg                                                                                                                         <  2:1366397/148‑1 (MQ=255)

                             aCCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTgg                                                                                                                        >  1:1057445/1‑148 (MQ=255)

                                aGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCa                                                                                                                     >  2:2916637/1‑148 (MQ=255)

                                 gCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTAACCTTTTTTGTGACCCATCAGTTCCAGACCGATATTTGCCAg                                                                                                                    >  1:211859/1‑148 (MQ=255)

                                 gCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTAAGGTAAAGATATTAACCAGTTTTGTGACCCAACAGTTCCAGACCGATATTGGCCAg                                                                                                                    >  1:1248010/1‑148 (MQ=255)

                                   ttAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCa                                                                                                                  <  2:373269/148‑1 (MQ=255)

                                      aTCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACtt                                                                                                               <  2:2013142/148‑1 (MQ=255)

                                        cAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCg                                                                                                             >  2:329188/1‑148 (MQ=255)

                                        cAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCg                                                                                                             <  1:3292933/148‑1 (MQ=255)

                                         aGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGt                                                                                                            <  2:373163/148‑1 (MQ=255)

                                         aGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGt                                                                                                            <  2:2039035/148‑1 (MQ=255)

                                            gAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCTCCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTgg                                                                                                         <  1:3010312/148‑1 (MQ=255)

                                            gAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTgg                                                                                                         <  1:1455793/148‑1 (MQ=255)

                                                   aaaCTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTAAGGTACTGATATTAACCATTTTTGTGACCCAAAAGTTCCAGAACCATATTTTCCAGCACATCGTTGGTTTTTAt                                                                                                  >  1:2280770/1‑148 (MQ=255)

                                                    aaCTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATg                                                                                                 >  2:1673958/1‑148 (MQ=255)

                                                      cTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGtt                                                                                               <  1:1666825/148‑1 (MQ=255)

                                                        gCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTAACCGTTTTGGTGAACCAACAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTa                                                                                             >  1:1016661/1‑148 (MQ=255)

                                                           aTACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTAACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACgg                                                                                          >  2:1715106/1‑148 (MQ=255)

                                                            tACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGa                                                                                         <  1:1989992/148‑1 (MQ=255)

                                                             aCGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGaa                                                                                        >  2:1875672/1‑148 (MQ=255)

                                                              cGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAg                                                                                       <  1:3246593/148‑1 (MQ=255)

                                                              cGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATGGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAg                                                                                       <  1:1144505/148‑1 (MQ=255)

                                                                gAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTa                                                                                     <  2:160797/148‑1 (MQ=255)

                                                                gAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTa                                                                                     <  2:1831811/148‑1 (MQ=255)

                                                                 aaaCCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTAc                                                                                    >  1:1380960/1‑148 (MQ=255)

                                                                 aaaCCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCAACAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTAc                                                                                    >  1:2208011/1‑148 (MQ=255)

                                                                 aaaCCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTAAGGTACAGATATTAACCTTTTTTGTGACCCAACAGTTACAGACCGATATTTGCCAGCACATAGTTGGTGTTGATGTTTTAGGAAGTAc                                                                                    >  1:2615765/1‑148 (MQ=255)

                                                                  aaCCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGGTTACGGAAGTAcc                                                                                   >  2:1566044/1‑148 (MQ=255)

                                                                           ggTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCgccgcc                                                                          <  1:1273240/148‑1 (MQ=255)

                                                                            gTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGccc                                                                         <  1:1594113/148‑1 (MQ=255)

                                                                               ggCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTAAGGTACAGATATTCACCTTTTTGGTGTCCCAACAGTTACAGACCCATATTGGCCAGCACTTGGTTTGTTTTAATGTTTAAGGAAGTACCAGCGCCGCCCTgg                                                                      >  1:713073/1‑148 (MQ=255)

                                                                                  gtcgtTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAg                                                                   <  1:1659001/148‑1 (MQ=255)

                                                                                   tcgtTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGa                                                                  >  2:808751/1‑148 (MQ=255)

                                                                                   tcgtTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCGTTTTTGTGACCCAACAGTTCCAGACCGATATTGGCCAGCCCTTCGTTGGTGTTCATGTTTAAGGAAGTACCAGCGCCGCCCAGGTAGa                                                                  >  1:532974/1‑148 (MQ=255)

                                                                                     gtTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTTGTGACCCAACAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACg                                                                >  1:241863/1‑148 (MQ=255)

                                                                                     gtTAGTGGACTGACATTTGTTAACAGGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACg                                                                <  2:942309/148‑1 (MQ=255)

                                                                                        aGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCt                                                             >  2:463808/1‑148 (MQ=255)

                                                                                          tGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGGTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTAc                                                           >  2:2738672/1‑148 (MQ=255)

                                                                                            gACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCg                                                         <  2:2555120/148‑1 (MQ=255)

                                                                                            gACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGGTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCg                                                         >  1:2022821/1‑148 (MQ=255)

                                                                                              cTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCggg                                                       <  1:2686324/148‑1 (MQ=255)

                                                                                                gACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGaa                                                     >  1:2592170/1‑148 (MQ=255)

                                                                                                  cATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTAACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACt                                                   >  2:44064/1‑148 (MQ=255)

                                                                                                   aTTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTg                                                  >  2:2965884/1‑148 (MQ=255)

                                                                                                    tttGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGa                                                 <  2:2851038/148‑1 (MQ=255)

                                                                                                    tttGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGa                                                 >  2:896003/1‑148 (MQ=255)

                                                                                                     ttGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGAt                                                >  2:2254063/1‑148 (MQ=255)

                                                                                                       gTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATcc                                              <  1:1132749/148‑1 (MQ=255)

                                                                                                           aCATGGTCGTTCGGGTTCAGGTACTGATATTAACCTTTTTTGTGACCCAACAGTTCCAGACCGATATTGGCCAGCACATCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCAGGTAGACGTCTACCGGGAACTGATACAAGc                                          >  2:1159572/1‑148 (MQ=255)

                                                                                                            cATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCa                                         <  2:125325/148‑1 (MQ=255)

                                                                                                              tGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCAtt                                       <  1:2684193/148‑1 (MQ=255)

                                                                                                               ggTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCAttt                                      <  2:706494/148‑1 (MQ=255)

                                                                                                                gTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCAtttt                                     >  2:686542/1‑148 (MQ=255)

                                                                                                                gTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCAtttt                                     >  1:2892542/1‑148 (MQ=255)

                                                                                                                   gTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCATTTTCCg                                  <  2:3039121/148‑1 (MQ=255)

                                                                                                                     tCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCATTTTCCgtt                                <  2:2278356/148‑1 (MQ=255)

                                                                                                                      cGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCATTTTCCgttg                               <  2:210209/148‑1 (MQ=255)

                                                                                                                      cGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCATTTTCCgttg                               <  1:2456527/148‑1 (MQ=255)

                                                                                                                        ggTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCATTTTCCgttgtt                             <  1:930504/148‑1 (MQ=255)

                                                                                                                         gTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCATTTTCCGTTGTTc                            <  2:3052077/148‑1 (MQ=255)

                                                                                                                         gTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCATTTTCCGTTGTTc                            >  1:1861402/1‑148 (MQ=255)

                                                                                                                           tCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCATTTTCCGTTGTTCAg                          >  2:2351378/1‑148 (MQ=255)

                                                                                                                           tCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCATTTTCCGTTGTTCAg                          >  2:3084679/1‑148 (MQ=255)

                                                                                                                            cAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCATTTTCCGTTGTTCAgg                         >  2:2328089/1‑148 (MQ=255)

                                                                                                                             aGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCATTTTCCGTTGTTCAGGa                        <  2:2191738/148‑1 (MQ=255)

                                                                                                                                tACTGATAGTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACGTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCATTTTCCGTTGTTCAGGACtt                     <  2:519014/148‑1 (MQ=255)

                                                                                                                                 aCTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCATTTTCCGTTGTTCAGGACTtc                    <  1:2112478/148‑1 (MQ=255)

                                                                                                                                 aCTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATACATGCATTTTCCGTTGTTAAGGACtta                    >  1:3186588/1‑147 (MQ=255)

                                                                                                                                  cTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCATTTTCCGTTGTTCAGGACTtca                   <  1:3005575/148‑1 (MQ=255)

                                                                                                                                   tGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCATTTTCCGTTGTTCAGGACTtcat                  >  1:2956800/1‑148 (MQ=255)

                                                                                                                                    gATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCATTTTCCGTTGTTCAGGACTtcatc                 >  2:2583050/1‑148 (MQ=255)

                                                                                                                                     atatTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCATTTTCCGTTGTTCAGGACTtcatca                >  1:802455/1‑148 (MQ=255)

                                                                                                                                     atatTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCATTTTCCGTTGTTCAGGACTtcatca                >  2:2138140/1‑148 (MQ=255)

                                                                                                                                      tatTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCATTTTCCGTTGTTCAGGACTTCATcac               >  2:551232/1‑148 (MQ=255)

                                                                                                                                        ttCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCATTTTCCGTTGTTCAGGACTTCATCACAt             >  1:2336943/1‑148 (MQ=255)

                                                                                                                                            ccTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCATTTTCCGTTGTTCAGGACTTCATCACATGCgg         >  1:1293160/1‑148 (MQ=255)

                                                                                                                                              tttttGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCATTTTCCGTTGTTCAGGACTTCATCACATGCGGCa       <  2:3201038/148‑1 (MQ=255)

                                                                                                                                               ttttGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCATTTTCCGTTGTTCAGGACTTCATCACATGCGGCaa      <  1:648406/148‑1 (MQ=255)

                                                                                                                                                tttGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCATTTTCCGTTGTTCAGGACTTCATCACATGCGGCAat     <  1:3310286/148‑1 (MQ=255)

                                                                                                                                                  tGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCATTTTCCGTTGTTCAGGACTTCATCACATGCGGCAatga   <  1:2711124/148‑1 (MQ=255)

                                                                                                                                                  tGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCATTTTCCGTTGTTCAGGACTTCATCACATGCGGCAatga   <  1:3083484/148‑1 (MQ=255)

AGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTTGGTGTTCATGTTTACGGAAGTACCTGCGCCGCCCTGGTAGACGTCTACCGGGAACTGATCCATGCATTTTCCGTTGTTCAGGACTTCATCACATGCGGCAATGAT  >  CP009273/4357625‑4357919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`