Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,372,049 C→A 15.3% P29P (CCG→CCT)  frdA ← fumarate reductase (anaerobic) catalytic and NAD/flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,372,0490CA15.3% 693.5 / 73.6 228P29P (CCG→CCT) frdAfumarate reductase (anaerobic) catalytic and NAD/flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base C (127/66);  new base A (28/7);  total (155/73)
Fisher's exact test for biased strand distribution p-value = 1.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

Only 100 of 229 total aligned reads displayed.

ACACAACCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAG  >  CP009273/4371902‑4372195
                                                                                                                                                   |                                                                                                                                                  
  acaACCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCgg                                                                                                                                                  <  1:2777687/148‑1 (MQ=255)
   caAACAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCTAAGATGTCATGATCCTGCGCGACAGAGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGa                                                                                                                                                 >  1:1961450/1‑148 (MQ=255)
          tCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCt                                                                                                                                          >  1:591898/1‑148 (MQ=255)
          tCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCt                                                                                                                                          <  1:920531/148‑1 (MQ=255)
               aCCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGcgcg                                                                                                                                     >  1:1436197/1‑148 (MQ=255)
                    cTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGgcagc                                                                                                                                >  1:2499951/1‑148 (MQ=255)
                     tACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGgcagca                                                                                                                               >  1:1282899/1‑148 (MQ=255)
                     tACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTAGGATTTGCCTGCGCGgcagca                                                                                                                               <  1:1615002/148‑1 (MQ=255)
                      aCTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTAGGATTTGCCTGCGCGgcagcac                                                                                                                              >  1:3141831/1‑147 (MQ=255)
                       cTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAAt                                                                                                                             >  1:787223/1‑148 (MQ=255)
                        tGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAAtt                                                                                                                            <  1:1552166/148‑1 (MQ=255)
                        tGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAAtt                                                                                                                            >  2:693110/1‑148 (MQ=255)
                               tGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCAc                                                                                                                     >  2:467901/1‑148 (MQ=255)
                                     tGATATTCGCAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATc                                                                                                               <  2:2674587/148‑1 (MQ=255)
                                     tGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATc                                                                                                               <  2:1110418/148‑1 (MQ=255)
                                     tGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATc                                                                                                               <  2:764049/148‑1 (MQ=255)
                                     tGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATc                                                                                                               >  2:10665/1‑148 (MQ=255)
                                      gATATTCGAAGCTGTCATGATCCTGCGAGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTAGAGAATAGTCCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATca                                                                                                              >  2:2484732/1‑147 (MQ=255)
                                         atTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGNTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCcgc                                                                                                           >  1:1682010/1‑148 (MQ=255)
                                         atTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCcgc                                                                                                           >  1:214955/1‑148 (MQ=255)
                                          ttCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCcgcg                                                                                                          >  2:3255284/1‑148 (MQ=255)
                                          ttCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCcgcg                                                                                                          >  2:1770731/1‑148 (MQ=255)
                                          ttCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCcgcg                                                                                                          >  1:941897/1‑148 (MQ=255)
                                              aaGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCAc                                                                                                      >  1:1792640/1‑148 (MQ=255)
                                              aaGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCAc                                                                                                      >  1:2259054/1‑148 (MQ=255)
                                                gCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCg                                                                                                    >  2:119133/1‑148 (MQ=255)
                                                gCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTAGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCg                                                                                                    >  1:3106137/1‑148 (MQ=255)
                                                 cTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCgg                                                                                                   >  2:572893/1‑148 (MQ=255)
                                                    tCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTGGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGgcgc                                                                                                <  1:444585/148‑1 (MQ=255)
                                                    tCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCACTTGCAGCACGTAATCCCGCGCCACCGgcgc                                                                                                >  1:3086615/1‑148 (MQ=255)
                                                    tCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTAGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGgcgc                                                                                                >  2:2574634/1‑148 (MQ=255)
                                                      aTGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCt                                                                                              <  1:2934050/148‑1 (MQ=255)
                                                       tGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTAGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTa                                                                                             >  2:1455856/1‑148 (MQ=255)
                                                         aTCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACa                                                                                           <  2:2115557/148‑1 (MQ=255)
                                                             tGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATgg                                                                                       >  2:2800728/1‑148 (MQ=255)
                                                                   aCAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGAt                                                                                 >  2:3293169/1‑148 (MQ=255)
                                                                    cAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTAGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATc                                                                                >  1:1193054/1‑148 (MQ=255)
                                                                       cggcggAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGc                                                                             <  2:2846866/148‑1 (MQ=255)
                                                                       cggcggAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGc                                                                             <  2:1533766/148‑1 (MQ=255)
                                                                         gcggAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCtt                                                                           <  2:224059/148‑1 (MQ=255)
                                                                          cggAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTAGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTg                                                                          >  1:2730789/1‑148 (MQ=255)
                                                                             aGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGaaa                                                                       >  1:2881117/1‑148 (MQ=255)
                                                                             aGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGaaa                                                                       >  1:266819/1‑148 (MQ=255)
                                                                               cccccTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAgg                                                                     <  2:942894/148‑1 (MQ=255)
                                                                                ccccTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGt                                                                    <  1:549371/148‑1 (MQ=255)
                                                                                 cccTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGtt                                                                   >  2:1800921/1‑148 (MQ=255)
                                                                                  ccTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGttt                                                                  >  1:2942987/1‑148 (MQ=255)
                                                                                  ccTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGttt                                                                  >  2:2459224/1‑148 (MQ=255)
                                                                                  ccTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTAGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGttt                                                                  >  1:173566/1‑148 (MQ=255)
                                                                                     tCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCa                                                               <  1:10665/148‑1 (MQ=255)
                                                                                       tGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACg                                                             <  2:195352/148‑1 (MQ=255)
                                                                                         cagcaACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGAc                                                           <  1:1800921/148‑1 (MQ=255)
                                                                                           gcaACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTAGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACAt                                                         >  1:635968/1‑148 (MQ=255)
                                                                                            caACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACAtt                                                        >  1:1862480/1‑148 (MQ=255)
                                                                                            caACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACAtt                                                        >  2:3092592/1‑148 (MQ=255)
                                                                                            caACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTAGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACAtt                                                        >  1:3271665/1‑148 (MQ=255)
                                                                                             aaCGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATtc                                                       <  1:1308104/148‑1 (MQ=255)
                                                                                                ggTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATtcctc                                                    <  1:121950/148‑1 (MQ=255)
                                                                                                ggTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATtcctc                                                    <  2:2925856/148‑1 (MQ=255)
                                                                                                  tATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCa                                                  >  1:1879925/1‑148 (MQ=255)
                                                                                                  tATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCa                                                  >  1:2185144/1‑148 (MQ=255)
                                                                                                   aTGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAg                                                 >  2:1540464/1‑148 (MQ=255)
                                                                                                   aTGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTAGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAg                                                 <  2:2312376/148‑1 (MQ=255)
                                                                                                       cTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTg                                             >  2:726839/1‑148 (MQ=255)
                                                                                                       cTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTg                                             >  1:3071430/1‑148 (MQ=255)
                                                                                                         aCGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGtt                                           >  1:104909/1‑148 (MQ=255)
                                                                                                         aCGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGtt                                           >  1:1965082/1‑148 (MQ=255)
                                                                                                         aCGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGtt                                           <  1:2704049/148‑1 (MQ=255)
                                                                                                         aCGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGtt                                           >  1:2825604/1‑148 (MQ=255)
                                                                                                          cGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGttt                                          <  2:1961450/148‑1 (MQ=255)
                                                                                                          cGCATCGGGTATACTGTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGttt                                          <  2:2185933/148‑1 (MQ=255)
                                                                                                                  gTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCca                                  <  2:162003/148‑1 (MQ=255)
                                                                                                                  gTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCca                                  >  1:979302/1‑148 (MQ=255)
                                                                                                                   tataCTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCcac                                 >  1:1466328/1‑148 (MQ=255)
                                                                                                                    ataCTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGAGTGTTTTTATCCcaca                                >  1:455823/1‑148 (MQ=255)
                                                                                                                     taCTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAg                               >  2:704922/1‑148 (MQ=255)
                                                                                                                     taCTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAg                               >  2:834714/1‑148 (MQ=255)
                                                                                                                     taCTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAg                               >  2:950391/1‑148 (MQ=255)
                                                                                                                      aCTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGc                              >  1:820630/1‑148 (MQ=255)
                                                                                                                      aCTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGc                              >  1:1075499/1‑148 (MQ=255)
                                                                                                                       cTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGcc                             <  2:2229374/148‑1 (MQ=255)
                                                                                                                       cTTTTGAGATTAGTGCGATTTTTGCATTAGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGcc                             >  2:1972723/1‑148 (MQ=255)
                                                                                                                         tttGAGATTAGTGCGATTTTTGCATTAGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCAc                           >  1:725683/1‑148 (MQ=255)
                                                                                                                          ttGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACg                          >  2:1315387/1‑148 (MQ=255)
                                                                                                                          ttGAGATTAGTGCGATTTTTGCATTAGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACg                          >  1:2491326/1‑148 (MQ=255)
                                                                                                                              gaTTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACt                      >  1:3219650/1‑148 (MQ=255)
                                                                                                                               aTTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACtt                     >  2:896904/1‑148 (MQ=255)
                                                                                                                                ttAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTc                    >  1:2924594/1‑148 (MQ=255)
                                                                                                                                 tAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCa                   <  2:1633349/148‑1 (MQ=255)
                                                                                                                                     gCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGt               >  1:3262716/1‑148 (MQ=255)
                                                                                                                                      cGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTa              >  2:927276/1‑148 (MQ=255)
                                                                                                                                       gATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTaa             <  1:2122545/148‑1 (MQ=255)
                                                                                                                                       gATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTaa             <  1:2185803/148‑1 (MQ=255)
                                                                                                                                         tttttGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGt           >  2:3192247/1‑148 (MQ=255)
                                                                                                                                          ttttGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTa          >  2:1621641/1‑148 (MQ=255)
                                                                                                                                          ttttGCATTAGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTa          <  1:1382893/148‑1 (MQ=255)
                                                                                                                                               cATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGa     <  1:924153/148‑1 (MQ=255)
                                                                                                                                               cATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGa     <  1:2578829/148‑1 (MQ=255)
                                                                                                                                                aTTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGaa    >  2:909055/1‑148 (MQ=255)
                                                                                                                                                  tCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAg  <  2:3025921/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACACAACCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAG  >  CP009273/4371902‑4372195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑