| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 361,806 | G→A | 100% | intergenic (‑63/+14) | lacI ← / ← mhpR | DNA‑binding transcriptional repressor/mhp operon transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 361,806 | 0 | G | A | 97.2% | 22384.2 / inf | 6834 | intergenic (‑63/+14) | lacI/mhpR | DNA‑binding transcriptional repressor/mhp operon transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base G (84/107); new base A (3454/3183); total (3540/3294) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.29e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01 | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 6838 total aligned reads displayed.
GCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGC > CP009273/361660‑361953 | gcCTGGTTCACCACGCGGGAAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCAt > 2:2884752/1‑148 (MQ=255) gcCTGGTTCACCACGCGGGAAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCAt > 1:723960/1‑148 (MQ=255) cTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCg > 1:1001095/1‑148 (MQ=255) tGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGa > 2:875149/1‑148 (MQ=255) gggTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGa < 1:2133693/147‑1 (MQ=255) ggTTCTCCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGAt > 1:4642/1‑148 (MQ=255) ttCACCACGCGGGAAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATg < 2:456750/148‑1 (MQ=255) ttCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATgg > 2:1785182/1‑148 (MQ=255) ttCACCACGCGGG‑AAAAGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATgg < 2:2393112/148‑1 (MQ=255) tCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGgt < 2:1406592/148‑1 (MQ=255) tCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGgt < 2:2248583/148‑1 (MQ=255) caccacGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGgtg > 2:516607/1‑148 (MQ=255) caccacGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACANTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGgtg > 1:1682613/1‑148 (MQ=255) accacGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTAACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGgtgt > 1:2773568/1‑148 (MQ=255) ccacGCGGGAAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGgtgt < 1:2647129/148‑1 (MQ=255) ccacGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTc > 2:2452415/1‑148 (MQ=255) cacGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCa < 2:1837243/148‑1 (MQ=255) acGCGGGAAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCa < 2:320163/148‑1 (MQ=255) cgcgGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAt > 1:1740699‑M2/1‑147 (MQ=255) cgcgGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAt > 2:3068165‑M2/1‑147 (MQ=255) ggg‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaa > 2:1215791‑M2/1‑144 (MQ=255) gg‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaat > 1:1462550‑M2/1‑143 (MQ=255) g‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGCTGGTGTCAAttaatt < 2:1277850‑M2/148‑7 (MQ=255) aaCGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaa > 2:2628676‑M2/1‑140 (MQ=255) cGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaag > 1:2867608‑M2/1‑138 (MQ=255) ggTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaagg > 1:1867183‑M2/1‑137 (MQ=255) ggTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaagg < 2:2507376‑M2/148‑12 (MQ=255) gTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggc > 2:1473153‑M2/1‑136 (MQ=255) tCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggca > 2:1896895‑M2/1‑135 (MQ=255) tCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAgtaattaaaggca < 2:2023272‑M2/148‑14 (MQ=255) tCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAatgcatgc < 1:3084003/148‑1 (MQ=255) cTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcat > 2:342535‑M2/1‑134 (MQ=255) cTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcat > 2:671133‑M2/1‑134 (MQ=255) tatAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCCAttaattaaaggcatca < 2:3141861‑M2/147‑17 (MQ=255) gATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatca < 1:675971‑M2/148‑17 (MQ=255) aTAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCt < 1:320955/148‑1 (MQ=255) aTAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaa > 2:108609‑M2/1‑131 (MQ=255) tAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaa < 1:236466‑M2/148‑19 (MQ=255) aaGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaat > 2:2995935‑M2/1‑129 (MQ=255) gagaCACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataa < 1:3270565‑M2/148‑22 (MQ=255) gaCACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaa > 1:1287395‑M2/1‑125 (MQ=255) ccGGCATACTCTGCGAGATCGTATAACGTTACTGGTTTCACATTCACCACACTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTAGATGGTGTCAAttaattaaaggcatcaaataaaacgaa > 2:1969943‑M2/1‑121 (MQ=255) ccGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaa > 2:1532248‑M2/1‑121 (MQ=255) ccGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaa > 1:570958‑M2/1‑121 (MQ=255) gCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaagg > 1:2188275‑M2/1‑118 (MQ=255) gCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAACGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaagg < 1:2965496‑M2/148‑31 (MQ=255) cATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggc > 2:3269867‑M2/1‑117 (MQ=255) cATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggc < 1:3150064‑M2/148‑32 (MQ=255) tACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctc > 1:1057436‑M2/1‑115 (MQ=255) aCTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctca < 2:2576551‑M2/148‑35 (MQ=255) aCTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctca < 2:614506‑M2/148‑35 (MQ=255) tctGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGa < 1:2048833/148‑1 (MQ=255) tctGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagt > 2:564159‑M2/1‑112 (MQ=255) cGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaa > 2:1155069‑M2/1‑108 (MQ=255) aCATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaag < 1:1986693‑M2/148‑43 (MQ=255) aCATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaag > 1:1222902‑M2/1‑106 (MQ=255) cATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCt < 2:2829696/148‑1 (MQ=255) cATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaaga < 1:1341936‑M2/148‑44 (MQ=255) cATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaaga > 2:270379‑M2/1‑105 (MQ=255) tataACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTAGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactggg < 1:2474632‑M2/148‑49 (MQ=255) ataACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggc > 2:1026573‑M2/1‑99 (MQ=255) taACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataacacgaaaggctcagtcgaaagactgggcc > 1:1922629‑M2/1‑98 (MQ=255) taACGTTACTGGTATCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTAACATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc > 2:2939773‑M2/1‑98 (MQ=255) aaCGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcct > 1:960494‑M2/1‑97 (MQ=255) aaCGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcct > 1:1798829‑M2/1‑97 (MQ=255) aaCGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcct < 2:1721758‑M2/148‑52 (MQ=255) cGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttt > 1:896106‑M2/1‑95 (MQ=255) cGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTGCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttt > 2:2316338‑M2/1‑95 (MQ=255) tACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt < 1:3027744‑M2/148‑57 (MQ=255) ggTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta > 2:2190737‑M2/1‑88 (MQ=255) ggTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta > 2:404459‑M2/1‑88 (MQ=255) ggTTTAACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAGGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta > 2:1597740‑M2/1‑88 (MQ=255) tttCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatc < 1:1628489‑M2/148‑63 (MQ=255) ttCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct < 1:1165069‑M2/148‑64 (MQ=255) tCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt < 2:808403‑M2/148‑71 (MQ=255) caccacCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttg > 1:616274‑M2/1‑77 (MQ=255) caccacCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttg > 1:2709889‑M2/1‑77 (MQ=255) ccaccCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttttc < 1:5221‑M2/148‑74 (MQ=255) ccaccCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc < 1:2422700‑M2/148‑74 (MQ=255) cccTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggt < 2:1341648‑M2/148‑77 (MQ=255) cTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga > 2:213126‑M2/1‑70 (MQ=255) gAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac < 2:743584‑M2/148‑81 (MQ=255) aTTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc > 1:2821825‑M2/1‑66 (MQ=255) ttGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct > 2:942785‑M2/1‑65 (MQ=255) tGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc < 2:2750228‑M2/148‑85 (MQ=255) aCTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAg < 1:831638/148‑1 (MQ=255) aCTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc > 2:1655886‑M2/1‑62 (MQ=255) aCTCTCTTCCGGGCGCTATCATGCCATAACGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc < 1:783390‑M2/148‑87 (MQ=255) ctctctTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcc > 1:25227‑M2/1‑61 (MQ=255) ggCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggac < 1:2310249‑M2/148‑98 (MQ=255) gcgcTATCATGCCATACCGCGAAAGGTTTTGCACCATTTGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggaca > 1:2913335‑M2/1‑50 (MQ=255) cgcTATCATGCCATACCGCGAAAGGTTTTGCACCATTAGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaa > 1:41081‑M2/1‑49 (MQ=255) cTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaat < 1:1520985‑M2/148‑102 (MQ=255) tATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcgtttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatc < 1:139979‑M2/148‑103 (MQ=255) tCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccg > 1:1577745‑M2/1‑44 (MQ=255) cATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgc < 1:2243814‑M2/148‑106 (MQ=255) aTACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgccct < 2:2084076‑M2/148‑112 (MQ=255) tACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgcccta < 1:1114108‑M2/148‑113 (MQ=255) aCCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgccctag > 1:340082‑M2/1‑35 (MQ=255) ccGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgccctaga < 1:2588174‑M2/148‑115 (MQ=255) | GCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGC > CP009273/361660‑361953 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |