BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,417,122	G→T	84.9%	R40S (CGT→AGT)	yjfZ ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,417,122	0	G	T	84.9%	490.4 / 95.2	242	R40S (CGT→AGT)	yjfZ	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (22/15); new base T (104/98); total (129/113)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 242 total aligned reads displayed.

CATCCAAAAGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTT  >  CP009273/4416977‑4417264

 atccaAAAGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATTATCTCTTCCACTAtt                                                                                                                                             >  1:2780365/1‑148 (MQ=255)

  tccaAAAGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACGTATTGAATGCTTTCCAGTATGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTAttt                                                                                                                                            >  1:3217609/1‑148 (MQ=255)

     aaaaGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGaa                                                                                                                                         <  2:849243/148‑1 (MQ=255)

     aaaaGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGaa                                                                                                                                         <  2:2398560/148‑1 (MQ=255)

      aaaGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGGTTGTGGATCTCAACAGTACGTCTGCCATCGCAAGTATGATATCATCCACTATTTGaaa                                                                                                                                        >  1:95793/1‑148 (MQ=255)

         gAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTa                                                                                                                                     <  1:2220111/148‑1 (MQ=255)

           aTTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAAt                                                                                                                                   <  1:1816613/148‑1 (MQ=255)

            ttATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATg                                                                                                                                  <  2:2780722/148‑1 (MQ=255)

               tCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAg                                                                                                                               <  2:1120349/148‑1 (MQ=255)

               tCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAg                                                                                                                               <  2:3122779/148‑1 (MQ=255)

                  ccATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAgcgc                                                                                                                            >  1:1736892/1‑148 (MQ=255)

                   cattcattGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGcgcg                                                                                                                           >  2:62846/1‑148 (MQ=255)

                   cattcattGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGcgcg                                                                                                                           >  2:1264206/1‑148 (MQ=255)

                    attcattGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCgcgt                                                                                                                          >  1:527878/1‑148 (MQ=255)

                      tcattGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCgcgtgc                                                                                                                        >  1:2649081/1‑148 (MQ=255)

                      tcattGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCgcgtgc                                                                                                                        >  1:1550476/1‑148 (MQ=255)

                        attGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCgcgtgcgt                                                                                                                      <  2:3073699/148‑1 (MQ=255)

                         ttGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATTCTTGCCAGTCTGAGATATCTAGTTTGTGGATCTCAAAAGTACGTCTGCCATCGCAATTATTATATCTTCCACAATTTGAAAGTAATGTAGCgcgtgcgga                                                                                                                     >  1:1521474/1‑146 (MQ=255)

                          tgtgATTCCATCGTAAGCATTACACCTCGTCGCTAACATATTGAAATCTTGCCACTCAGACAGATCGAGTTTGTGGTTCTCAAAAGTACCTCTGCCAACGCAATTATTAACTCTTACACGAATTGAAAGTAATTTAGCGCGGGCGTAt                                                                                                                    >  1:1954624/1‑148 (MQ=255)

                           gtgATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTAtt                                                                                                                   >  2:2960408/1‑148 (MQ=255)

                             gATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATtga                                                                                                                 >  2:788738/1‑148 (MQ=255)

                              aTTCCATCGTAATCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATtgat                                                                                                                <  1:427268/148‑1 (MQ=255)

                                   aTCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaat                                                                                                           <  1:435341/148‑1 (MQ=255)

                                    tCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATATCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaatt                                                                                                          >  1:144736/1‑148 (MQ=255)

                                    tCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTATGCCATAGCAAATATGATCTCTTCCACAATTTGAAAGTAATGTATCGCGTGCGTATTGATGAaatt                                                                                                          >  1:1610084/1‑148 (MQ=255)

                                     cGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaattt                                                                                                         <  2:24487/148‑1 (MQ=255)

                                       tAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATATCAACAGTACGTCTGCCATCGCAATTATTATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaatttta                                                                                                       >  1:2014976/1‑148 (MQ=255)

                                        aaGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaattttaa                                                                                                      >  2:945601/1‑148 (MQ=255)

                                          gCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCCCGTTTTGAAAGTAATGTATCGCGTGCGTATTGATGAaattttaatt                                                                                                    >  2:1970721/1‑148 (MQ=255)

                                          gCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaattttaatt                                                                                                    <  1:1173220/148‑1 (MQ=255)

                                          gCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaattttaatt                                                                                                    >  2:2086088/1‑148 (MQ=255)

                                           cATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaattttaattt                                                                                                   <  1:2556279/148‑1 (MQ=255)

                                             ttACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAattttaatttta                                                                                                 <  1:2814002/148‑1 (MQ=255)

                                              tACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTAc                                                                                                >  2:2303122/1‑148 (MQ=255)

                                                cacCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTg                                                                                              >  2:1630388/1‑148 (MQ=255)

                                                cacCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATATCAACAGTACGTCTGCCATCGCAATTATGATATCTTCCACAATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTAATg                                                                                              >  1:826523/1‑148 (MQ=255)

                                                 acCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTgg                                                                                             <  1:731657/148‑1 (MQ=255)

                                                     cgtcgCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTg                                                                                         >  1:2485979/1‑148 (MQ=255)

                                                        cgCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCg                                                                                      >  1:2646182/1‑148 (MQ=255)

                                                            aaCTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTgg                                                                                  <  2:1848807/148‑1 (MQ=255)

                                                            aaCTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTgg                                                                                  >  1:657021/1‑148 (MQ=255)

                                                              cTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGAc                                                                                >  1:2971889/1‑148 (MQ=255)

                                                              cTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGAc                                                                                >  2:9503/1‑148 (MQ=255)

                                                                     aaTGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAAc                                                                         >  2:219375/1‑148 (MQ=255)

                                                                        gCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACgag                                                                      <  2:200959/148‑1 (MQ=255)

                                                                         cTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTACTTTTACTGGTTTGTCGGTGGACGAAAAACgaga                                                                     <  2:1736892/148‑1 (MQ=255)

                                                                            gCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATaa                                                                  <  1:3075191/148‑1 (MQ=255)

                                                                             ccAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATaaa                                                                 <  2:896759/148‑1 (MQ=255)

                                                                              cAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAat                                                                >  2:1410281/1‑148 (MQ=255)

                                                                              cAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAat                                                                >  2:1325947/1‑148 (MQ=255)

                                                                              cAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAat                                                                >  2:2682383/1‑148 (MQ=255)

                                                                               aGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAata                                                               <  1:796131/148‑1 (MQ=255)

                                                                               aGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTACACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAata                                                               <  1:2354476/148‑1 (MQ=255)

                                                                                gTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAatat                                                              <  1:34728/148‑1 (MQ=255)

                                                                                 tCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATg                                                             >  2:702981/1‑148 (MQ=255)

                                                                                  cTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGc                                                            <  2:1767148/148‑1 (MQ=255)

                                                                                   tGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCa                                                           <  1:2775577/148‑1 (MQ=255)

                                                                                     agagaTCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAgg                                                         >  2:1364321/1‑148 (MQ=255)

                                                                                     agagaTCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAgg                                                         >  2:2580992/1‑148 (MQ=255)

                                                                                       agaTCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGaa                                                       >  1:293699/1‑148 (MQ=255)

                                                                                       agaTCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGaa                                                       <  1:219375/148‑1 (MQ=255)

                                                                                        gaTCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGaaa                                                      <  2:1958185/148‑1 (MQ=255)

                                                                                         aTCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAAt                                                     >  1:900261/1‑148 (MQ=255)

                                                                                          tCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATg                                                    >  2:1017323/1‑148 (MQ=255)

                                                                                          tCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATg                                                    <  2:498480/148‑1 (MQ=255)

                                                                                           cTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGa                                                   <  1:1280826/148‑1 (MQ=255)

                                                                                            tAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGaa                                                  <  1:2578779/148‑1 (MQ=255)

                                                                                             aGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGaat                                                 >  2:1582171/1‑148 (MQ=255)

                                                                                              gTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGaata                                                >  2:562471/1‑148 (MQ=255)

                                                                                               tttGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGaataa                                               >  2:2155246/1‑148 (MQ=255)

                                                                                               tttGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGaataa                                               >  1:2379531/1‑148 (MQ=255)

                                                                                                  gtgGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGaataaata                                            <  1:1699535/148‑1 (MQ=255)

                                                                                                      aTCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCg                                        >  2:3202676/1‑148 (MQ=255)

                                                                                                       tctcAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGt                                       <  1:2915813/148‑1 (MQ=255)

                                                                                                        ctcAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGtt                                      >  1:1300198/1‑148 (MQ=255)

                                                                                                         tcAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTg                                     >  1:2113048/1‑148 (MQ=255)

                                                                                                            aCAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATCAATAGTCGTTGGTa                                  >  1:174873/1‑148 (MQ=255)

                                                                                                            aCAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTa                                  <  2:2335886/148‑1 (MQ=255)

                                                                                                            aCAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTa                                  >  2:1919746/1‑148 (MQ=255)

                                                                                                                tccGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGt                              <  2:2572169/146‑1 (MQ=255)

                                                                                                                  cGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCa                            <  2:894512/148‑1 (MQ=255)

                                                                                                                     cTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTg                         >  1:1409568/1‑148 (MQ=255)

                                                                                                                         cATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAAt                     >  2:3259900/1‑148 (MQ=255)

                                                                                                                          aTCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATg                    >  1:596689/1‑148 (MQ=255)

                                                                                                                          aTCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATg                    >  1:930395/1‑148 (MQ=255)

                                                                                                                          aTCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATg                    <  1:2095605/148‑1 (MQ=255)

                                                                                                                            cGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGtt                  <  1:1017323/148‑1 (MQ=255)

                                                                                                                            cGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGtt                  <  2:2856022/148‑1 (MQ=255)

                                                                                                                            cGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGtt                  <  1:1017199/148‑1 (MQ=255)

                                                                                                                             gCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTa                 <  2:146808/148‑1 (MQ=255)

                                                                                                                               aaTTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTAcc               <  2:527878/148‑1 (MQ=255)

                                                                                                                                 ttATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTg             >  2:2505805/1‑148 (MQ=255)

                                                                                                                                  tATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGt            <  2:78037/148‑1 (MQ=255)

                                                                                                                                     gATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTa         >  2:411830/1‑148 (MQ=255)

                                                                                                                                      aTCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTaa        >  2:570291/1‑148 (MQ=255)

                                                                                                                                        ctctTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATg      <  1:2249837/148‑1 (MQ=255)

                                                                                                                                         tctTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGt     <  1:1327436/148‑1 (MQ=255)

                                                                                                                                            tCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGtttt  <  1:3207765/148‑1 (MQ=255)

                                                                                                                                            tCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGtttt  <  2:1313097/148‑1 (MQ=255)

                                                                                                                                            tCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGtttt  >  2:2982851/1‑148 (MQ=255)

CATCCAAAAGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTT  >  CP009273/4416977‑4417264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`