Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 362,159 G→T 100% I165I (ATC→ATA)  mhpR ← mhp operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273362,1590GT99.5% 638.2 / ‑4.2 199I165I (ATC→ATA) mhpRmhp operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (91/106);  total (92/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 199 total aligned reads displayed.

CGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATT  >  CP009273/362014‑362303
                                                                                                                                                 |                                                                                                                                                
     gagGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAAtt                                                                                                                                           >  2:996050/1‑148 (MQ=255)
      agGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTc                                                                                                                                          <  2:2800484/148‑1 (MQ=255)
       ggCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTcc                                                                                                                                         <  2:506060/148‑1 (MQ=255)
       ggCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTcc                                                                                                                                         <  1:64679/148‑1 (MQ=255)
        gCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCt                                                                                                                                        <  1:1421325/148‑1 (MQ=255)
        gCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCt                                                                                                                                        >  1:2846596/1‑148 (MQ=255)
         cGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTCACATTTCTATTAATTCCtt                                                                                                                                       >  2:1969591/1‑148 (MQ=255)
         cGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCtt                                                                                                                                       >  1:2410387/1‑148 (MQ=255)
           aTCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGc                                                                                                                                     >  1:2103148/1‑148 (MQ=255)
            tcttctCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCg                                                                                                                                    >  1:959940/1‑148 (MQ=255)
            tcttctCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCg                                                                                                                                    >  1:1752547/1‑148 (MQ=255)
                ctcctcCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCt                                                                                                                                <  2:294686/148‑1 (MQ=255)
                  cctcctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTg                                                                                                                              <  1:3205070/148‑1 (MQ=255)
                  cctcctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTg                                                                                                                              >  2:2438940/1‑148 (MQ=255)
                  cctcctGATCCCAGCCGCGGGAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTg                                                                                                                              <  1:996671/148‑1 (MQ=255)
                   ctcctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGt                                                                                                                             <  1:770428/148‑1 (MQ=255)
                    tcctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGtt                                                                                                                            >  2:3035471/1‑148 (MQ=255)
                     cctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTc                                                                                                                           >  1:2014466/1‑148 (MQ=255)
                       tGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCg                                                                                                                         <  2:559165/148‑1 (MQ=255)
                       tGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCg                                                                                                                         >  1:1312884/1‑148 (MQ=255)
                        gATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCgg                                                                                                                        >  1:2655939/1‑148 (MQ=255)
                         aTCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCggg                                                                                                                       <  2:555658/148‑1 (MQ=255)
                          tCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGc                                                                                                                      >  2:673506/1‑148 (MQ=255)
                          tCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGc                                                                                                                      <  1:2277712/148‑1 (MQ=255)
                            ccAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCaa                                                                                                                    >  1:3002423/1‑148 (MQ=255)
                                     gTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagc                                                                                                           <  2:1534674/148‑1 (MQ=255)
                                     gTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagc                                                                                                           <  2:2738650/148‑1 (MQ=255)
                                      tAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagcc                                                                                                          >  2:1521358/1‑148 (MQ=255)
                                      tAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagcc                                                                                                          >  2:1817769/1‑148 (MQ=255)
                                      tAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagcc                                                                                                          >  1:2231750/1‑148 (MQ=255)
                                      tAGTTCTGGCCGGAACCCTCTTTGCGCGCGCGCGCCAGACTGGCGTCCAGCTTTAACGGGTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagcc                                                                                                          <  2:1236416/148‑1 (MQ=255)
                                       aGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagcca                                                                                                         <  2:1490336/148‑1 (MQ=255)
                                        gTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGccagccag                                                                                                        <  2:1930380/148‑1 (MQ=255)
                                         ttCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAgg                                                                                                       <  1:1571384/148‑1 (MQ=255)
                                           cTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTc                                                                                                     <  1:1047739/148‑1 (MQ=255)
                                              tCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTCACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAgg                                                                                                  >  1:2392616/1‑148 (MQ=255)
                                              tCCGTAACCCTCTTGGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAgg                                                                                                  <  2:1011750/148‑1 (MQ=255)
                                               ccGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGc                                                                                                 <  1:2218537/148‑1 (MQ=255)
                                               ccGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGc                                                                                                 >  1:2966672/1‑148 (MQ=255)
                                               ccGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGc                                                                                                 >  2:1121430/1‑148 (MQ=255)
                                                  tAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCg                                                                                              >  1:2088910/1‑148 (MQ=255)
                                                  tAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCg                                                                                              <  1:1719536/148‑1 (MQ=255)
                                                   aaCCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGa                                                                                             >  2:631355/1‑148 (MQ=255)
                                                    aCCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAg                                                                                            >  2:3206002/1‑148 (MQ=255)
                                                     cccTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAgg                                                                                           >  2:2323997/1‑148 (MQ=255)
                                                     cccTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAgg                                                                                           <  1:442764/148‑1 (MQ=255)
                                                     cccTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAgg                                                                                           >  1:1024520/1‑148 (MQ=255)
                                                     cccTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATAACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAgg                                                                                           >  2:2261966/1‑148 (MQ=255)
                                                      ccTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCCTCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGc                                                                                          >  2:2500535/1‑148 (MQ=255)
                                                        tctTTGCGCGCGCGCGCCAGAATGGCTTCAAGCTTTAAAGGTTCCCGTGCAAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCGTGCGGTCTTGTTCCGGGCAAAATGCCAGCCAGGTCAGGCCCGAGGCgg                                                                                        >  1:2130576/1‑148 (MQ=255)
                                                         ctTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGt                                                                                       >  1:326779/1‑148 (MQ=255)
                                                         ctTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGt                                                                                       >  1:2403319/1‑148 (MQ=255)
                                                         ctTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGt                                                                                       >  1:2403583/1‑148 (MQ=255)
                                                         ctTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGt                                                                                       >  1:1918648/1‑148 (MQ=255)
                                                          tttGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGtt                                                                                      <  1:861254/148‑1 (MQ=255)
                                                          tttGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGGTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGtt                                                                                      <  1:2517058/148‑1 (MQ=255)
                                                            tGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGtttt                                                                                    <  2:1650347/148‑1 (MQ=255)
                                                                cgcgcgcgcCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTGAGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGa                                                                                >  2:2659333/1‑148 (MQ=255)
                                                                   gcgcgcCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGc                                                                             <  1:1836819/148‑1 (MQ=255)
                                                                        ccAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCaa                                                                        <  1:1590999/148‑1 (MQ=255)
                                                                         cAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCaaa                                                                       <  1:779272/148‑1 (MQ=255)
                                                                         cAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCaaa                                                                       <  1:1277811/148‑1 (MQ=255)
                                                                           gAATGGCGTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACg                                                                     <  2:2588668/148‑1 (MQ=255)
                                                                               ggCTTCCAGATTTAACGGGTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAAGTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGc                                                                 <  1:1987447/148‑1 (MQ=255)
                                                                                gCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGcc                                                                <  1:2440775/148‑1 (MQ=255)
                                                                                gCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGcc                                                                >  2:1705820/1‑148 (MQ=255)
                                                                                gCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGcc                                                                <  1:781826/148‑1 (MQ=255)
                                                                                   tCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGa                                                             <  1:2071885/148‑1 (MQ=255)
                                                                                       gCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCAt                                                         >  1:2925307/1‑148 (MQ=255)
                                                                                       gCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCAt                                                         >  1:3115212/1‑148 (MQ=255)
                                                                                         tttAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTg                                                       <  1:1658128/148‑1 (MQ=255)
                                                                                           tAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGcc                                                     <  2:1110414/148‑1 (MQ=255)
                                                                                             aCGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCg                                                   >  1:3071964/1‑148 (MQ=255)
                                                                                             aCGGTTCCCGTGCCAGTTGAGAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCg                                                   <  1:2789167/148‑1 (MQ=255)
                                                                                              cGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCgg                                                  <  2:2379385/148‑1 (MQ=255)
                                                                                                 ttCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGa                                               >  2:466532/1‑148 (MQ=255)
                                                                                                  tCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGaa                                              <  2:2014461/148‑1 (MQ=255)
                                                                                                  tCCCGTGCCAGTTGAGAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGaa                                              <  2:2865877/148‑1 (MQ=255)
                                                                                                   cccGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGaaa                                             <  1:541554/148‑1 (MQ=255)
                                                                                                     cGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAgg                                           <  2:2917052/148‑1 (MQ=255)
                                                                                                             ttGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCgg                                   <  1:1826846/148‑1 (MQ=255)
                                                                                                             ttGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCgg                                   <  2:768382/148‑1 (MQ=255)
                                                                                                             ttGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCgg                                   <  1:386283/148‑1 (MQ=255)
                                                                                                              tGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGc                                  >  1:932345/1‑148 (MQ=255)
                                                                                                               gATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCt                                 <  2:2790513/148‑1 (MQ=255)
                                                                                                                  aGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAAGTCCTGGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGaa                              <  2:1432020/148‑1 (MQ=255)
                                                                                                                      atcaCCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACgg                          <  2:2843597/148‑1 (MQ=255)
                                                                                                                      atcaCCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACgg                          <  2:844164/148‑1 (MQ=255)
                                                                                                                        caCCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTg                        <  2:2024694/148‑1 (MQ=255)
                                                                                                                             gggCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTgg                   <  1:139119/148‑1 (MQ=255)
                                                                                                                               gCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGtt                 <  2:2230816/148‑1 (MQ=255)
                                                                                                                                  ggAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTcg              <  2:3114461/148‑1 (MQ=255)
                                                                                                                                    aGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTcgcg            >  2:2349760/1‑148 (MQ=255)
                                                                                                                                    aGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTcgcg            >  2:689149/1‑148 (MQ=255)
                                                                                                                                     ggCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGt           <  2:2231750/148‑1 (MQ=255)
                                                                                                                                       cTACCATTTCTATTAATGCCTTGCGGTCTTGTTCCGGGCAAAAGGCCCGCCTGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGttc         <  2:2597773/148‑3 (MQ=255)
                                                                                                                                       cTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTac         >  2:135742/1‑148 (MQ=255)
                                                                                                                                            aTTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTAccacca    <  2:2403583/148‑1 (MQ=255)
                                                                                                                                              ttCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCAtt  >  1:1978288/1‑148 (MQ=255)
                                                                                                                                              ttCTATTAATTCCTTCCGGTCTTGTTCCGGGCAAAAGGACAGCCAGGTCAGGCCCGAGGCGGTTTTAATAATCGGCAAACGTCTCCCGACCAATTCCCGGTGAAAGGATAATATGCTGAAACACTGAGAGGTTTAGCCTaccacaaat  >  1:582731/1‑144 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATT  >  CP009273/362014‑362303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑