Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,428,092 G→T 90.2% G190V (GGA→GTA)  ytfI → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,428,0920GT90.2% 632.9 / 65.3 246G190V (GGA→GTA) ytfIuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (13/11);  new base T (120/102);  total (133/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.28e-01

Only 100 of 248 total aligned reads displayed.

TATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTT  >  CP009273/4427946‑4428236
                                                                                                                                                  |                                                                                                                                                
tatTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCagga                                                                                                                                                 <  2:948559/148‑1 (MQ=255)
   tCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGt                                                                                                                                              >  2:42832/1‑148 (MQ=255)
      aCAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGa                                                                                                                                           <  1:1545021/148‑1 (MQ=255)
      aCAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGa                                                                                                                                           <  1:1696864/148‑1 (MQ=255)
       cAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGaa                                                                                                                                          >  1:1153669/1‑148 (MQ=255)
          aaaaaaCAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGGGTCTTTAGGTTATGCCTTATTGGCGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTa                                                                                                                                       >  2:36984/1‑148 (MQ=255)
            aaaaCAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTaaa                                                                                                                                     >  1:709122/1‑148 (MQ=255)
             aaaCAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAg                                                                                                                                    >  1:659318/1‑148 (MQ=255)
             aaaCAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAg                                                                                                                                    <  1:874854/148‑1 (MQ=255)
              aaCAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGa                                                                                                                                   <  1:437604/148‑1 (MQ=255)
                cAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAAt                                                                                                                                 <  1:3073284/148‑1 (MQ=255)
                cAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAAt                                                                                                                                 <  2:2054636/148‑1 (MQ=255)
                  gTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGc                                                                                                                               >  2:1924825/1‑148 (MQ=255)
                    cTGACATACCGAAAATGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTTTTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCga                                                                                                                             <  2:2803508/148‑1 (MQ=255)
                    cTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCga                                                                                                                             <  1:748267/148‑1 (MQ=255)
                    cTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCga                                                                                                                             <  2:3288515/148‑1 (MQ=255)
                    cTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCga                                                                                                                             <  2:1822825/148‑1 (MQ=255)
                        cATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCgatgga                                                                                                                         <  1:304946/148‑1 (MQ=255)
                         aTACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCgatggat                                                                                                                        >  1:1885159/1‑148 (MQ=255)
                          tACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCgatggatg                                                                                                                       <  2:2093193/148‑1 (MQ=255)
                          tACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCgatggatg                                                                                                                       >  1:2180261/1‑148 (MQ=255)
                           aCCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGatggatgg                                                                                                                      >  1:2262305/1‑148 (MQ=255)
                           aCCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGatggatgg                                                                                                                      >  2:2409429/1‑148 (MQ=255)
                              gAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGtat                                                                                                                   <  1:1275651/148‑1 (MQ=255)
                                aaaGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTatat                                                                                                                 <  2:1521983/148‑1 (MQ=255)
                                aaaGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTatat                                                                                                                 <  2:1323312/148‑1 (MQ=255)
                                aaaGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTatat                                                                                                                 <  2:1249749/148‑1 (MQ=255)
                                 aaGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATAtt                                                                                                                >  2:1152215/1‑148 (MQ=255)
                                  aGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAANCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATAttt                                                                                                               >  1:1676656/1‑148 (MQ=255)
                                   ggATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTa                                                                                                              >  2:2661771/1‑148 (MQ=255)
                                   ggATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTa                                                                                                              >  2:1138373/1‑148 (MQ=255)
                                          aGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGaaaaa                                                                                                       <  2:1698797/148‑1 (MQ=255)
                                             ttATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAaata                                                                                                    >  2:515111/1‑148 (MQ=255)
                                              tataTTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAaataa                                                                                                   >  1:287030/1‑148 (MQ=255)
                                                 atTCACCAGGCGAATTGTGACGTTTCTTTAGGTTATGCCTTATTGTAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATATACGAATAAATAATtg                                                                                                >  2:3303032/1‑148 (MQ=255)
                                                      ccAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTgatgat                                                                                           >  2:680798/1‑148 (MQ=255)
                                                       cAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATa                                                                                          >  2:2592156/1‑148 (MQ=255)
                                                          gCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAAtt                                                                                       >  2:1018200/1‑148 (MQ=255)
                                                             aTTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTg                                                                                    >  2:2771957/1‑148 (MQ=255)
                                                               tGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCCAAGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTgac                                                                                  >  2:137516/1‑146 (MQ=255)
                                                                ggTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCa                                                                                 <  1:1400302/148‑1 (MQ=255)
                                                                    aCGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGt                                                                             <  2:3039302/148‑1 (MQ=255)
                                                                     cGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTc                                                                            >  2:898692/1‑148 (MQ=255)
                                                                      gtgtCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCa                                                                           <  2:1264633/148‑1 (MQ=255)
                                                                      gtgtCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCa                                                                           <  2:2289118/148‑1 (MQ=255)
                                                                      gtgtCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCa                                                                           <  2:2943090/148‑1 (MQ=255)
                                                                      gtgtCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCa                                                                           <  2:1626069/148‑1 (MQ=255)
                                                                        gtCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATa                                                                         >  1:2873803/1‑148 (MQ=255)
                                                                         tCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAg                                                                        >  2:595062/1‑148 (MQ=255)
                                                                          cTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGa                                                                       >  2:1282914/1‑148 (MQ=255)
                                                                          cTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGa                                                                       <  1:1138373/148‑1 (MQ=255)
                                                                              aGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCt                                                                   <  1:2267130/148‑1 (MQ=255)
                                                                               ggTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCtt                                                                  >  2:1841226/1‑148 (MQ=255)
                                                                               ggTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCtt                                                                  >  2:139296/1‑148 (MQ=255)
                                                                                gTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCttt                                                                 >  2:1409689/1‑148 (MQ=255)
                                                                                  tATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTgg                                                               >  1:1066633/1‑148 (MQ=255)
                                                                                  tATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTgg                                                               >  2:2447272/1‑148 (MQ=255)
                                                                                    tGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGc                                                             >  2:482960/1‑148 (MQ=255)
                                                                                     gCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCt                                                            <  1:1096588/148‑1 (MQ=255)
                                                                                        ttATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTg                                                         <  2:571561/148‑1 (MQ=255)
                                                                                         tATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTgc                                                        >  1:2436094/1‑148 (MQ=255)
                                                                                         tATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTgc                                                        >  2:1064949/1‑148 (MQ=255)
                                                                                         tATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTgc                                                        >  1:950724/1‑148 (MQ=255)
                                                                                           ttGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTgcgc                                                      >  2:2891695/1‑148 (MQ=255)
                                                                                                ggaTATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTaaa                                                 <  2:1650890/148‑1 (MQ=255)
                                                                                                 gaTATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTaaaa                                                >  1:2783277/1‑148 (MQ=255)
                                                                                                   tataGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAAta                                              >  2:1904780/1‑147 (MQ=255)
                                                                                                     taGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTa                                            <  2:2105628/148‑1 (MQ=255)
                                                                                                          aGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAg                                       >  1:2636070/1‑148 (MQ=255)
                                                                                                          aGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAg                                       >  1:2829656/1‑148 (MQ=255)
                                                                                                            cAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAggg                                     >  2:2189588/1‑148 (MQ=255)
                                                                                                            cAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAggg                                     >  1:1318725/1‑148 (MQ=255)
                                                                                                             aaaTTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAgggg                                    >  1:2183575/1‑148 (MQ=255)
                                                                                                              aaTTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGTCTTTGCGCTTAAAATCTATGGAGGGGa                                   <  1:1596177/148‑1 (MQ=255)
                                                                                                                ttAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATg                                 >  2:2737190/1‑148 (MQ=255)
                                                                                                                 tAAAACGGTGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGa                                <  1:2539524/148‑1 (MQ=255)
                                                                                                                    aaCGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACg                             <  1:1894156/148‑1 (MQ=255)
                                                                                                                    aaCGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACg                             >  2:2038607/1‑148 (MQ=255)
                                                                                                                    aaCGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACg                             <  1:42832/148‑1 (MQ=255)
                                                                                                                     aCGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGt                            >  1:1828151/1‑148 (MQ=255)
                                                                                                                         ggTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTc                        >  2:3124845/1‑148 (MQ=255)
                                                                                                                            aaaTTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTa                     <  1:2409429/148‑1 (MQ=255)
                                                                                                                                 tGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGt                <  1:1118849/148‑1 (MQ=255)
                                                                                                                                    aCTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTcc             <  2:1676656/148‑1 (MQ=255)
                                                                                                                                     cTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCct            >  2:731814/1‑148 (MQ=255)
                                                                                                                                      tCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCctc           <  1:542796/148‑1 (MQ=255)
                                                                                                                                      tCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCctc           <  1:815899/148‑1 (MQ=255)
                                                                                                                                      tCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCctc           >  2:121346/1‑148 (MQ=255)
                                                                                                                                        cAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCtg         <  1:1185491/148‑1 (MQ=255)
                                                                                                                                        cAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCtg         <  2:829606/148‑1 (MQ=255)
                                                                                                                                        cAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCtg         <  1:829235/148‑1 (MQ=255)
                                                                                                                                          aaTACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCtgtt       <  2:950741/148‑1 (MQ=255)
                                                                                                                                             aCCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTgttgtt    >  1:3246627/1‑148 (MQ=255)
                                                                                                                                              ccAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGttt   <  1:1724761/148‑1 (MQ=255)
                                                                                                                                               cAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGtttt  >  2:1770889/1‑148 (MQ=255)
                                                                                                                                               cAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGtttt  >  2:1771056/1‑148 (MQ=255)
                                                                                                                                               cAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGtttt  >  2:3168572/1‑148 (MQ=255)
                                                                                                                                               cAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGtttt  >  2:3168531/1‑148 (MQ=255)
                                                                                                                                               cAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGtttt  <  1:2581879/148‑1 (MQ=255)
                                                                                                                                               cAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGtttt  <  1:361050/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTT  >  CP009273/4427946‑4428236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑