Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,470,873 G→T 86.4% R928S (CGT→AGT)  valS ← valyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,470,8730GT86.4% 329.2 / 56.4 155R928S (CGT→AGT) valSvalyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (11/10);  new base T (69/65);  total (80/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-01

Only 100 of 160 total aligned reads displayed.

TTTACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGGCCAGC  >  CP009273/4470728‑4471017
                                                                                                                                                 |                                                                                                                                                
tttACCATTTCTGTAAGATATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCActct                                                                                                                                                <  1:2748020/148‑1 (MQ=255)
 ttACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGctc                                                                                                                                               >  1:2425437/1‑148 (MQ=255)
  tACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCActctct                                                                                                                                              >  2:1138078/1‑148 (MQ=255)
   aCCATTTCTGCAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCATCGATTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCtt                                                                                                                                             <  2:451967/148‑1 (MQ=255)
    ccATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCttt                                                                                                                                            <  2:2759280/148‑1 (MQ=255)
    ccATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCttt                                                                                                                                            <  2:3166939/148‑1 (MQ=255)
      aTTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTcg                                                                                                                                          >  2:565090/1‑148 (MQ=255)
      aTTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTcg                                                                                                                                          >  1:2145964/1‑148 (MQ=255)
      aTTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTcg                                                                                                                                          >  1:2146343/1‑148 (MQ=255)
      aTTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTcg                                                                                                                                          >  2:108160/1‑148 (MQ=255)
      aTTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACGGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCtttag                                                                                                                                          >  2:565039/1‑146 (MQ=255)
            gTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGa                                                                                                                                    >  1:1373357/1‑148 (MQ=255)
            gTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGa                                                                                                                                    >  2:1537752/1‑148 (MQ=255)
            gTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGa                                                                                                                                    <  1:1929591/148‑1 (MQ=255)
             tAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGAc                                                                                                                                   >  1:3119771/1‑148 (MQ=255)
              aaGAGATAAAAAAGGCCGGAGCATGCTCCGGCGTTCGTTTTCATCACGGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGTcc                                                                                                                                  >  2:673328/1‑148 (MQ=255)
               agagATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCg                                                                                                                                 >  2:1409978/1‑148 (MQ=255)
                 agaTAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCt                                                                                                                               >  2:1550592/1‑148 (MQ=255)
                  gaTAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCtt                                                                                                                              <  2:3170884/148‑1 (MQ=255)
                  gaTAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCtt                                                                                                                              <  1:714309/148‑1 (MQ=255)
                    tAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTcc                                                                                                                            <  2:3301835/148‑1 (MQ=255)
                          aGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTgcg                                                                                                                      <  2:2317374/148‑1 (MQ=255)
                          aGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTgcg                                                                                                                      <  2:2317254/148‑1 (MQ=255)
                          aGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTgcg                                                                                                                      >  2:682119/1‑148 (MQ=255)
                          aGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTgcg                                                                                                                      >  2:723900/1‑148 (MQ=255)
                          aGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTgcg                                                                                                                      >  1:3089741/1‑148 (MQ=255)
                                   cATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACa                                                                                                             >  1:529926/1‑148 (MQ=255)
                                     tGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACaaa                                                                                                           >  2:454321/1‑148 (MQ=255)
                                      gCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCACACTCTCTTTAGCGATGACCGCTTCCGGTGCGCGGGCGACAAAg                                                                                                          >  1:59977/1‑148 (MQ=255)
                                            gCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCttcg                                                                                                    <  1:1079372/148‑1 (MQ=255)
                                            gCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCttcg                                                                                                    <  1:1079022/148‑1 (MQ=255)
                                             ccTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCttcgt                                                                                                   <  2:2563284/148‑1 (MQ=255)
                                             ccTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCttcgt                                                                                                   >  1:2769121/1‑148 (MQ=255)
                                              cTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCatcgtt                                                                                                  >  1:2915918/1‑148 (MQ=255)
                                                    tttCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAg                                                                                            >  1:410588/1‑148 (MQ=255)
                                                    tttCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAg                                                                                            >  1:411480/1‑148 (MQ=255)
                                                        atcaCTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTg                                                                                        <  2:3089741/148‑1 (MQ=255)
                                                          caCTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGtt                                                                                      <  2:1886992/148‑1 (MQ=255)
                                                           aCTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTtc                                                                                     <  1:1648958/148‑1 (MQ=255)
                                                                 ttttGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATa                                                                               <  2:2218383/148‑1 (MQ=255)
                                                                 ttttGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATa                                                                               >  2:134931/1‑148 (MQ=255)
                                                                        tACAGCGCGGCGATAACAGCCTGCTGTTCACTCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACCAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGa                                                                        >  2:386044/1‑148 (MQ=255)
                                                                        tACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGa                                                                        <  2:668549/148‑1 (MQ=255)
                                                                        tACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCGTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGa                                                                        >  2:2684918/1‑148 (MQ=255)
                                                                        tACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGa                                                                        >  2:630374/1‑148 (MQ=255)
                                                                         aCAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGAt                                                                       <  1:2674448/148‑1 (MQ=255)
                                                                          cAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGGTCGCCAGTTTGTTCTCGATACGGCTGAtt                                                                      >  1:2666998/1‑148 (MQ=255)
                                                                            gcgcGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTc                                                                    >  2:69497/1‑148 (MQ=255)
                                                                               cggcgATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCAcc                                                                 <  2:1064543/148‑1 (MQ=255)
                                                                               cggcgATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCAcc                                                                 <  1:2388116/148‑1 (MQ=255)
                                                                               cggcgATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGAGGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCAcc                                                                 <  1:2607424/148‑1 (MQ=255)
                                                                                    aTAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCaa                                                            >  2:1704473/1‑148 (MQ=255)
                                                                                    aTAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCaa                                                            >  2:1704484/1‑148 (MQ=255)
                                                                                    aTAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCaa                                                            >  2:1171070/1‑148 (MQ=255)
                                                                                     tAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAAt                                                           >  2:410666/1‑148 (MQ=255)
                                                                                     tAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAAt                                                           >  2:2702232/1‑148 (MQ=255)
                                                                                      aaCAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATc                                                          <  1:2776806/148‑1 (MQ=255)
                                                                                      aaCAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATc                                                          <  2:382956/148‑1 (MQ=255)
                                                                                          gCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCg                                                      >  1:995328/1‑148 (MQ=255)
                                                                                           ccTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGc                                                     >  2:1884012/1‑148 (MQ=255)
                                                                                           ccTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGc                                                     <  2:1694430/148‑1 (MQ=255)
                                                                                           ccTGCTGTTCAATCAGTTTCGCTTTCGCGTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGc                                                     >  1:960988/1‑148 (MQ=255)
                                                                                              gctgTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCAc                                                  >  2:2705348/1‑148 (MQ=255)
                                                                                              gctgTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCAc                                                  >  2:2705297/1‑148 (MQ=255)
                                                                                              gctgTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCAc                                                  <  2:1896029/148‑1 (MQ=255)
                                                                                              gctgTTCAATCAGTTTCGCTTTCGCATCCGCATAGCCTTCAAGCTTCTCACTCTATTTAGCGATGACAGCTTCCGGTTCGCGGGCGACAAAGCCATCGTTCGCCAGTTTGTTCTCTATACGTCTGATTTCACCATCAATCTAAGCCAc                                                  >  2:345285/1‑148 (MQ=255)
                                                                                                 gTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCActtc                                               <  1:2569253/148‑1 (MQ=255)
                                                                                                   tCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCActtctt                                             <  2:1123800/148‑1 (MQ=255)
                                                                                                   tCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCActtctt                                             <  1:443225/148‑1 (MQ=255)
                                                                                                   tCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCActtctt                                             <  2:1122980/148‑1 (MQ=255)
                                                                                                    cAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCttt                                            <  2:2146343/148‑1 (MQ=255)
                                                                                                     aaTCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTc                                           >  1:2918968/1‑148 (MQ=255)
                                                                                                        cAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGcc                                        >  2:1989931/1‑148 (MQ=255)
                                                                                                        cAGTTTCGCTTTCGCTTCCCCATAGCCATCCACCTTCTCACTCTCTTTCGCCATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTTTTCTAGATAAGGCTGATTTAAACTTCAATATTCGCCACTACTTTCGcc                                        >  1:118424/1‑148 (MQ=255)
                                                                                                         aGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCa                                       <  2:308657/148‑1 (MQ=255)
                                                                                                             tcgctttcgcttCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGAcg                                   <  1:1409999/148‑1 (MQ=255)
                                                                                                              cgctttcgcttCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGAcgc                                  >  2:754267/1‑148 (MQ=255)
                                                                                                              cgctttcgcttCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGAcgc                                  >  2:2396571/1‑148 (MQ=255)
                                                                                                               gctttcgcttCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGAcgcg                                 <  1:3215744/148‑1 (MQ=255)
                                                                                                                ctttcgcttCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACgcgc                                <  2:1159488/148‑1 (MQ=255)
                                                                                                                  ttcgcttCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCAACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCa                              >  2:797890/1‑148 (MQ=255)
                                                                                                                        tCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCAt                        <  1:1537752/148‑1 (MQ=255)
                                                                                                                         ccGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCAtc                       <  1:2787989/148‑1 (MQ=255)
                                                                                                                         ccGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCAtc                       <  1:3299756/148‑1 (MQ=255)
                                                                                                                         ccGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCAtc                       >  2:800075/1‑148 (MQ=255)
                                                                                                                            cATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCAtcttc                    <  2:293161/148‑1 (MQ=255)
                                                                                                                             aTAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCAtcttct                   <  2:2442232/148‑1 (MQ=255)
                                                                                                                               aGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCttt                 <  2:2050490/148‑1 (MQ=255)
                                                                                                                                gCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTttg                <  1:3103535/148‑1 (MQ=255)
                                                                                                                                    tCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTtga            <  1:1550592/148‑1 (MQ=255)
                                                                                                                                    tCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTtga            >  2:2705045/1‑148 (MQ=255)
                                                                                                                                    tCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTtga            >  1:2813470/1‑148 (MQ=255)
                                                                                                                                      cAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTtgatg          >  2:2202643/1‑148 (MQ=255)
                                                                                                                                         cTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAgg       >  2:3185988/1‑148 (MQ=255)
                                                                                                                                         cTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAgg       <  2:2784269/148‑1 (MQ=255)
                                                                                                                                         cTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAgg       >  2:809037/1‑148 (MQ=255)
                                                                                                                                          ttCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGgc      <  1:1097924/148‑1 (MQ=255)
                                                                                                                                          ttCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGgc      >  1:2672711/1‑148 (MQ=255)
                                                                                                                                           tctcACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGACAGACGAGCCAGATCATATTCTTTGTTTATTAGgcc     >  2:871444/1‑148 (MQ=255)
                                                                                                                                              cACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGgccagc  >  1:2499217/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTTACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGGCCAGC  >  CP009273/4470728‑4471017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑