BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,472,936	G→T	13.7%	P240Q (CCG→CAG)	valS ←	valyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,472,936	0	G	T	13.7%	572.7 / 50.1	182	P240Q (CCG→CAG)	valS	valyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base G (91/65); new base T (13/12); total (105/77)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

Only 100 of 188 total aligned reads displayed.

ACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCTTTCGAT  >  CP009273/4472789‑4473079

aCTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAAcgcg                                                                                                                                                 >  2:1528185/1‑148 (MQ=255)

aCTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAAcgcg                                                                                                                                                 >  2:3167729/1‑148 (MQ=255)

aCTTCATAGTCGTGAAAGTCGTGCGCCGGAGGGATTTTAACGCCGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCCACGATCGGAAAACGAACGTTTACCAGCGGCAGAATTTCATATTTTCCAATAAGATCTTTGTAAcgcg                                                                                                                                                 >  1:3071021/1‑148 (MQ=255)

 cTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCgg                                                                                                                                                >  2:1086259/1‑148 (MQ=255)

       aGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTACATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACCTATTTGCCAATCAGATCTTTGTAACGCCGATCTTc                                                                                                                                          >  2:2058314/1‑148 (MQ=255)

         tCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCg                                                                                                                                        >  2:367771/1‑148 (MQ=255)

          cGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCgg                                                                                                                                       >  2:2897811/1‑148 (MQ=255)

           gTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCggg                                                                                                                                      <  2:1880896/148‑1 (MQ=255)

            ttAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGt                                                                                                                                     >  1:2650271/1‑148 (MQ=255)

             tAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGtt                                                                                                                                    >  1:1109055/1‑148 (MQ=255)

               aaGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTaa                                                                                                                                  <  1:1441818/148‑1 (MQ=255)

                aGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATAAGATCTTTGTAACGCGGATCTTCCGGGTTAAc                                                                                                                                 >  2:2044729/1‑148 (MQ=255)

                            gAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGt                                                                                                                     >  2:2324540/1‑148 (MQ=255)

                             aGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTa                                                                                                                    >  1:1505711/1‑148 (MQ=255)

                             aGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTa                                                                                                                    >  2:846357/1‑148 (MQ=255)

                             aGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTa                                                                                                                    <  2:2011244/148‑1 (MQ=255)

                               tGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATc                                                                                                                  >  2:1335019/1‑148 (MQ=255)

                                gATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCg                                                                                                                 >  1:1251563/1‑148 (MQ=255)

                                  ttttCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCNGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGcc                                                                                                               <  1:1677656/148‑1 (MQ=255)

                                  ttttCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGcc                                                                                                               <  1:1564518/148‑1 (MQ=255)

                                  ttttCACGCAGCCGGTGCCTTTGTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGcc                                                                                                               <  2:2372155/148‑1 (MQ=255)

                                   tttCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGccc                                                                                                              >  1:1359358/1‑148 (MQ=255)

                                       aCGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCcagca                                                                                                          >  1:2345029/1‑148 (MQ=255)

                                         gCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAgg                                                                                                        <  2:1254958/148‑1 (MQ=255)

                                               ggTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCTGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGtttat                                                                                                  <  1:2831478/148‑3 (MQ=255)

                                               ggTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCTGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGtttat                                                                                                  <  1:1183233/148‑3 (MQ=255)

                                                 tGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGTTCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCTGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTgg                                                                                                >  1:1515507/1‑148 (MQ=255)

                                                 tGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTgg                                                                                                >  1:1347413/1‑148 (MQ=255)

                                                  gCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCTGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGa                                                                                               <  1:401387/148‑1 (MQ=255)

                                                  gCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGa                                                                                               <  2:1671498/148‑1 (MQ=255)

                                                    cTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACg                                                                                             <  2:719781/148‑1 (MQ=255)

                                                    cTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACg                                                                                             <  2:3245437/148‑1 (MQ=255)

                                                    cTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACg                                                                                             >  2:2532638/1‑148 (MQ=255)

                                                     tttttCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACgg                                                                                            <  2:3038841/148‑1 (MQ=255)

                                                          ccATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGACTGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGt                                                                                       >  1:2541410/1‑148 (MQ=255)

                                                          ccATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGt                                                                                       >  2:989914/1‑148 (MQ=255)

                                                            aTGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTcg                                                                                     >  1:50930/1‑148 (MQ=255)

                                                             tGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCTGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTcgc                                                                                    >  2:1470823/1‑148 (MQ=255)

                                                              gTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCTGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTcgcg                                                                                   >  2:727720/1‑148 (MQ=255)

                                                                   cGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGaccac                                                                              <  1:1045655/148‑1 (MQ=255)

                                                                   cGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGaccac                                                                              >  1:898059/1‑148 (MQ=255)

                                                                      gtTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCTGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAg                                                                           <  2:2804043/148‑1 (MQ=255)

                                                                      gtTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAg                                                                           >  2:2529655/1‑148 (MQ=255)

                                                                      gtTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAg                                                                           <  2:2169820/148‑1 (MQ=255)

                                                                         cgtcgCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATa                                                                        <  2:176046/148‑1 (MQ=255)

                                                                           tcgCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAAt                                                                      <  1:1191990/148‑1 (MQ=255)

                                                                               cAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCttt                                                                  <  1:309650/148‑1 (MQ=255)

                                                                                aaCGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTGTa                                                                 >  2:1805284/1‑148 (MQ=255)

                                                                                 aCGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCTGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTAc                                                                <  1:2127429/148‑1 (MQ=255)

                                                                                  cGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTAcc                                                               >  2:3202936/1‑148 (MQ=255)

                                                                                  cGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTAcc                                                               >  1:786277/1‑148 (MQ=255)

                                                                                  cGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTAcc                                                               >  1:2444602/1‑148 (MQ=255)

                                                                                    aTCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCTGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGt                                                             <  1:2062526/148‑1 (MQ=255)

                                                                                           tACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGt                                                      <  1:1763831/148‑1 (MQ=255)

                                                                                           tACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGt                                                      >  1:821891/1‑148 (MQ=255)

                                                                                           tACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGt                                                      <  2:2774271/148‑1 (MQ=255)

                                                                                           tACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGt                                                      >  1:2503712/1‑148 (MQ=255)

                                                                                           tACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGt                                                      >  1:2504497/1‑148 (MQ=255)

                                                                                             cgacgGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGttt                                                    <  1:1970847/148‑1 (MQ=255)

                                                                                              gacgGTTAACCAGCGGCAGAATGACATATTTTCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGTTAATATTTACCGTATGCGGtttt                                                   <  2:2099797/148‑1 (MQ=255)

                                                                                              gacgGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGtttt                                                   <  1:1078645/148‑1 (MQ=255)

                                                                                                  gTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCa                                               >  2:834239/1‑148 (MQ=255)

                                                                                                  gTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCa                                               >  2:834030/1‑148 (MQ=255)

                                                                                                   ttAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCAc                                              <  1:1349094/148‑1 (MQ=255)

                                                                                                      aCCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGt                                           <  2:1217731/148‑1 (MQ=255)

                                                                                                       ccAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTc                                          <  2:2423798/148‑1 (MQ=255)

                                                                                                            ggCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTcagcca                                     <  2:2650271/148‑1 (MQ=255)

                                                                                                              cAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTcagccagc                                   >  1:3128295/1‑148 (MQ=255)

                                                                                                                 aaTGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCagcgga                                >  1:687145/1‑148 (MQ=255)

                                                                                                                  aTGACATATTTGCCAATCAGATCTTTGTAACGCTGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTCGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCagcggat                               >  1:3172067/1‑148 (MQ=255)

                                                                                                                  aTGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTCTCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCCGCCagcggat                               >  2:2251164/1‑148 (MQ=255)

                                                                                                                    gACATATTTGCCAATCAGATCTTTGTAACGCTGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCagcggatag                             >  2:2934194/1‑148 (MQ=255)

                                                                                                                    gACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATACTCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCagcggatag                             >  1:1280304/1‑148 (MQ=255)

                                                                                                                    gACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCTCACCGTCAGCCagcggatag                             >  2:2624610/1‑148 (MQ=255)

                                                                                                                       atatTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCagcggatagcgg                          <  2:1107379/148‑1 (MQ=255)

                                                                                                                        tatTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCagcggatagcgga                         >  2:736835/1‑148 (MQ=255)

                                                                                                                         atTTGCCAATCAGATCTTTGTAACGCTGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCagcggatagcggat                        >  1:392706/1‑148 (MQ=255)

                                                                                                                          tttGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGAtg                       <  1:164078/148‑1 (MQ=255)

                                                                                                                             gCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGc                    >  2:2968278/1‑148 (MQ=255)

                                                                                                                             gCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGc                    <  1:3009613/148‑1 (MQ=255)

                                                                                                                               cAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCca                  >  2:3304204/1‑148 (MQ=255)

                                                                                                                               cAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCca                  <  2:1621082/148‑1 (MQ=255)

                                                                                                                                 aTCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTCGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCcaca                >  1:180119/1‑148 (MQ=255)

                                                                                                                                  tCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACAt               >  1:226192/1‑148 (MQ=255)

                                                                                                                                   cAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATc              >  2:172603/1‑148 (MQ=255)

                                                                                                                                     gATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGa            >  1:989229/1‑148 (MQ=255)

                                                                                                                                     gATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGa            >  2:1420492/1‑148 (MQ=255)

                                                                                                                                      aTCTTTGTAACGCTGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGaa           >  2:251602/1‑148 (MQ=255)

                                                                                                                                      aTCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGaa           >  1:2409509/1‑148 (MQ=255)

                                                                                                                                      aTCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCATTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGaa           <  2:115605/148‑1 (MQ=255)

                                                                                                                                        cTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAAcc         <  2:3278744/148‑1 (MQ=255)

                                                                                                                                         tttGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCt        >  2:1622464/1‑148 (MQ=255)

                                                                                                                                         tttGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCt        <  2:3298106/148‑1 (MQ=255)

                                                                                                                                          ttGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCtt       <  1:2532638/148‑1 (MQ=255)

                                                                                                                                          ttGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCtt       <  2:1218233/148‑1 (MQ=255)

                                                                                                                                          ttGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCtt       <  2:1505746/148‑1 (MQ=255)

                                                                                                                                           tGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCttt      <  1:3281917/148‑1 (MQ=255)

                                                                                                                                             tAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCTTTCg    >  2:2503893/1‑148 (MQ=255)

                                                                                                                                               aCGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCTTTCGAt  >  1:362873/1‑148 (MQ=255)

                                                                                                                                               aCGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCTTTCGAt  >  1:908231/1‑148 (MQ=255)

ACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCTTTCGAT  >  CP009273/4472789‑4473079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`