Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,480,139 G→T 88.1% A273E (GCG→GAG)  idnR ← transcriptional repressor, 5‑gluconate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,480,1390GT88.1% 557.1 / 71.7 225A273E (GCG→GAG) idnRtranscriptional repressor, 5‑gluconate‑binding
Reads supporting (aligned to +/- strand):  ref base G (20/7);  new base T (106/92);  total (126/99)
Fisher's exact test for biased strand distribution p-value = 6.16e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 225 total aligned reads displayed.

GTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAA  >  CP009273/4479994‑4480282
                                                                                                                                                 |                                                                                                                                               
  cAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAAt                                                                                                                                             <  2:700375/148‑1 (MQ=255)
      aGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGaa                                                                                                                                         <  1:2378511/148‑1 (MQ=255)
      aGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGaa                                                                                                                                         <  2:754456/148‑1 (MQ=255)
       gttttgtggttgtgATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGaaa                                                                                                                                        <  2:601037/145‑1 (MQ=255)
       gtgtTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGaaa                                                                                                                                        <  1:1006613/148‑1 (MQ=255)
       gtgtTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGaaa                                                                                                                                        <  2:2836780/148‑1 (MQ=255)
        tgttgtGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAAt                                                                                                                                       <  1:1042391/148‑1 (MQ=255)
          ttgtggttgtgATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAAttt                                                                                                                                     >  1:332445/1‑148 (MQ=255)
           tgtggttgtgATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAAtgtg                                                                                                                                    <  2:1344598/148‑1 (MQ=255)
             tggttgtgATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGtt                                                                                                                                  >  1:535941/1‑148 (MQ=255)
              ggttgtgATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTc                                                                                                                                 <  2:2370674/148‑1 (MQ=255)
              ggttgtgATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTc                                                                                                                                 <  2:2295855/148‑1 (MQ=255)
              ggttgtgATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTc                                                                                                                                 <  1:974018/148‑1 (MQ=255)
                ttgtgATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTg                                                                                                                               >  1:3075358/1‑148 (MQ=255)
                    gATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCAc                                                                                                                           >  1:496987/1‑148 (MQ=255)
                     aTCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGCGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACg                                                                                                                          >  2:3164088/1‑148 (MQ=255)
                      tCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGCGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACgg                                                                                                                         >  1:1510459/1‑148 (MQ=255)
                      tCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACgg                                                                                                                         >  1:1397572/1‑148 (MQ=255)
                       cGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGc                                                                                                                        >  1:1591090/1‑148 (MQ=255)
                             ttttAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGtt                                                                                                                  >  2:2548202/1‑148 (MQ=255)
                             ttttAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATGTGTTCTGGCACGGCGAGGtt                                                                                                                  >  2:1251548/1‑148 (MQ=255)
                              tttAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGttt                                                                                                                 >  2:2959731/1‑148 (MQ=255)
                                tAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTtcg                                                                                                               <  1:2841229/148‑1 (MQ=255)
                                  aTTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTtcgct                                                                                                             <  2:1372715/148‑1 (MQ=255)
                                   ttttGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTGtcgctc                                                                                                            >  2:2063725/1‑148 (MQ=255)
                                    tttGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTtcgctcg                                                                                                           >  1:767266/1‑148 (MQ=255)
                                    tttGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTtcgctcg                                                                                                           >  1:1938051/1‑148 (MQ=255)
                                         tCAGCAACATTTGGGCGACCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGca                                                                                                      >  1:1687791/1‑148 (MQ=255)
                                          cAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGcac                                                                                                     <  2:2387795/148‑1 (MQ=255)
                                          cAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGcac                                                                                                     <  1:2549851/148‑1 (MQ=255)
                                            gCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCAcag                                                                                                   <  1:70841/148‑1 (MQ=255)
                                            gCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCAcag                                                                                                   <  1:2996064/148‑1 (MQ=255)
                                             cAACATTTGGGCGGACATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGATGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCAcagc                                                                                                  <  2:2127777/148‑1 (MQ=255)
                                                 aTTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGaa                                                                                              >  1:439902/1‑148 (MQ=255)
                                                  tttGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAg                                                                                             >  2:2663219/1‑148 (MQ=255)
                                                   ttGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAgc                                                                                            >  2:174184/1‑148 (MQ=255)
                                                   ttGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAgc                                                                                            >  2:2840226/1‑148 (MQ=255)
                                                   ttGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAgc                                                                                            >  2:1018727/1‑148 (MQ=255)
                                                   ttGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAgc                                                                                            >  2:2900274/1‑148 (MQ=255)
                                                   ttGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAgc                                                                                            >  2:432253/1‑148 (MQ=255)
                                                        cggcCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCAcc                                                                                       <  1:1864493/148‑1 (MQ=255)
                                                           ccATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCAt                                                                                    >  1:2253607/1‑148 (MQ=255)
                                                           ccATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCAt                                                                                    >  1:478311/1‑148 (MQ=255)
                                                               ccGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCa                                                                                >  1:1198636/1‑148 (MQ=255)
                                                               ccGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCCTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCa                                                                                >  1:1198679/1‑148 (MQ=255)
                                                                cGACCAATATCAAAACGGGGAGTACTTACGCTGGCGAGGCTTGGGATCAATTGCCTGCCAATCTCCAGCCCATGAAAACACTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCaa                                                                               <  2:2939687/148‑1 (MQ=255)
                                                                  aCCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAata                                                                             <  2:2645300/148‑1 (MQ=255)
                                                                  aCCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAata                                                                             >  2:2876775/1‑148 (MQ=255)
                                                                   ccAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAatat                                                                            <  1:1756757/148‑1 (MQ=255)
                                                                     aaTATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCg                                                                          <  2:1323884/148‑1 (MQ=255)
                                                                      atatCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGt                                                                         >  2:781061/1‑148 (MQ=255)
                                                                       tatCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTc                                                                        >  2:1356225/1‑148 (MQ=255)
                                                                         tCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCAt                                                                      <  2:2223163/148‑1 (MQ=255)
                                                                          cAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCAtt                                                                     >  2:615860/1‑148 (MQ=255)
                                                                            aaaCGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTgg                                                                   >  2:1111298/1‑148 (MQ=255)
                                                                             aaCGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGt                                                                  <  1:2548202/148‑1 (MQ=255)
                                                                                   gAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCaaaa                                                            <  2:1059377/148‑1 (MQ=255)
                                                                                    aGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAAt                                                           >  1:2152609/1‑148 (MQ=255)
                                                                                    aGTAATTACGCTGGCGAGGCGTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAAt                                                           <  1:1818744/148‑1 (MQ=255)
                                                                                      tAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATac                                                         <  2:1791041/148‑1 (MQ=255)
                                                                                       aaTTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATaca                                                        <  1:1299333/148‑1 (MQ=255)
                                                                                        aTTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATacac                                                       >  2:873493/1‑148 (MQ=255)
                                                                                           aCGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGt                                                    <  2:850683/148‑1 (MQ=255)
                                                                                           aCGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGt                                                    <  1:2109875/148‑1 (MQ=255)
                                                                                               tGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATGTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATGGTCATTGGTGCAAAATACACCGTCTaa                                                <  2:909382/148‑1 (MQ=255)
                                                                                                ggCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTaaa                                               <  2:2696930/148‑1 (MQ=255)
                                                                                                ggCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTaaa                                               <  2:2354763/148‑1 (MQ=255)
                                                                                                 gCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAAt                                              <  2:1510459/148‑1 (MQ=255)
                                                                                                    aGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCg                                           >  1:2414138/1‑148 (MQ=255)
                                                                                                      gCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCggg                                         >  1:1650232/1‑148 (MQ=255)
                                                                                                       cTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATGTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGt                                        <  2:1542670/148‑1 (MQ=255)
                                                                                                             aTCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTgcgc                                  <  2:2986777/148‑1 (MQ=255)
                                                                                                                aTTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTaa                               <  2:1185809/148‑1 (MQ=255)
                                                                                                                aTTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTaa                               <  2:383986/148‑1 (MQ=255)
                                                                                                                aTTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTaa                               <  2:2071920/148‑1 (MQ=255)
                                                                                                                  ttGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAgg                             >  1:2971961/1‑148 (MQ=255)
                                                                                                                  ttGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAgg                             >  1:2694230/1‑148 (MQ=255)
                                                                                                                  ttGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAgg                             >  1:1331473/1‑148 (MQ=255)
                                                                                                                    gcctgccAATCTCCAGCCCATGAAAACCCTCAATGGAAATGTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGc                           >  2:399334/1‑148 (MQ=255)
                                                                                                                      ctgccAATCTCCAGCCCATGAAAACCCTCAATGGAAATGTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCAt                         <  2:186472/148‑1 (MQ=255)
                                                                                                                          cAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACg                     >  2:2664714/1‑148 (MQ=255)
                                                                                                                           aaTCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGc                    >  2:3178579/1‑148 (MQ=255)
                                                                                                                            aTCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCa                   >  1:1713611/1‑148 (MQ=255)
                                                                                                                            aTCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCa                   <  1:2291650/148‑1 (MQ=255)
                                                                                                                            aTCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACAGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCa                   >  2:1483389/1‑148 (MQ=255)
                                                                                                                             tctcCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCAt                  >  2:626481/1‑148 (MQ=255)
                                                                                                                                ccAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTaa               <  1:3121625/148‑1 (MQ=255)
                                                                                                                                 cAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAAc              <  2:515643/148‑1 (MQ=255)
                                                                                                                                 cAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAAc              >  2:1143341/1‑148 (MQ=255)
                                                                                                                                 cAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAACACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAAc              >  2:1255353/1‑148 (MQ=255)
                                                                                                                                    cccATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGc           >  1:2324806/1‑148 (MQ=255)
                                                                                                                                      cATGAAAACCCTCAATGGAAATGTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCAt         >  1:1146087/1‑148 (MQ=255)
                                                                                                                                      cATGAAAACCCTCAATGGAAATGTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCAt         >  1:1146060/1‑148 (MQ=255)
                                                                                                                                        tGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTc       >  1:563203/1‑148 (MQ=255)
                                                                                                                                         gAAAACCAGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGAGCAAAATACACCGTCTAAATCCGTGTTTGCGCTAAGGGCATCACGCATTAACTGCATTcc      >  2:1489352/1‑148 (MQ=255)
                                                                                                                                           aaaCCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTa    >  1:2327562/1‑148 (MQ=255)
                                                                                                                                           aaaCCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTa    >  1:1255062/1‑148 (MQ=255)
                                                                                                                                           aaaCCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTa    >  2:1903116/1‑148 (MQ=255)
                                                                                                                                             aCCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTaaa  <  1:2013860/148‑1 (MQ=255)
                                                                                                                                             aCCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTaaa  >  2:815767/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAA  >  CP009273/4479994‑4480282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑