Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,494,721 G→A 92.6% A283T (GCC→ACC)  yjhB → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,494,7210GA92.6% 824.7 / 47.0 269A283T (GCC→ACC) yjhBputative transporter
Reads supporting (aligned to +/- strand):  ref base G (12/8);  new base A (117/132);  total (129/140)
Fisher's exact test for biased strand distribution p-value = 3.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.68e-01

Only 100 of 274 total aligned reads displayed.

ATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTTAT  >  CP009273/4494577‑4494866
                                                                                                                                                |                                                                                                                                                 
aTAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCt                                                                                                                                                >  2:141437/1‑148 (MQ=255)
 tAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCtt                                                                                                                                               >  2:3125389/1‑148 (MQ=255)
   aaCGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTg                                                                                                                                             >  1:1644815/1‑148 (MQ=255)
     cGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTa                                                                                                                                           >  2:258946/1‑148 (MQ=255)
       gACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGNTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGt                                                                                                                                         >  1:1677755/1‑148 (MQ=255)
          tactTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCgg                                                                                                                                      <  1:2638551/148‑1 (MQ=255)
          tacgTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCgg                                                                                                                                      <  1:846257/148‑1 (MQ=255)
           actTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGt                                                                                                                                     >  1:98539/1‑148 (MQ=255)
            cttccttcCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTc                                                                                                                                    <  2:2533488/148‑1 (MQ=255)
                 ttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCtaata                                                                                                                               <  1:2508786/148‑1 (MQ=255)
                  tcctccCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCtaataa                                                                                                                              <  2:3284474/148‑1 (MQ=255)
                             aGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCatttatt                                                                                                                   <  2:2006871/148‑1 (MQ=255)
                              gATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCatttattt                                                                                                                  <  1:1330942/148‑1 (MQ=255)
                              gATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCatttattt                                                                                                                  >  1:2962256/1‑148 (MQ=255)
                               aTAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAAGTTCATTTAtttt                                                                                                                 >  1:116860/1‑148 (MQ=255)
                               aTAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTAtttt                                                                                                                 >  1:1969553/1‑148 (MQ=255)
                                tAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTc                                                                                                                <  2:601340/148‑1 (MQ=255)
                                 aaTGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTcc                                                                                                               >  2:2790050/1‑148 (MQ=255)
                                    ggAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCttt                                                                                                            <  1:668829/148‑1 (MQ=255)
                                     gAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTg                                                                                                           >  1:1265888/1‑148 (MQ=255)
                                        ttAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTcc                                                                                                        <  1:1612078/148‑1 (MQ=255)
                                        ttAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTcc                                                                                                        <  2:1455633/148‑1 (MQ=255)
                                        ttAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTcc                                                                                                        >  2:3003619/1‑148 (MQ=255)
                                         tAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCct                                                                                                       <  1:1882637/148‑1 (MQ=255)
                                         tAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCct                                                                                                       <  1:210523/148‑1 (MQ=255)
                                             aCAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCttt                                                                                                   <  1:2772856/148‑1 (MQ=255)
                                                 tGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCttttttt                                                                                               <  2:272703/148‑1 (MQ=255)
                                                  ggTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCtttttttt                                                                                              <  1:2014734/148‑1 (MQ=255)
                                                   gTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTa                                                                                             >  1:1833614/1‑148 (MQ=255)
                                                     cATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTAtt                                                                                           >  1:581823/1‑148 (MQ=255)
                                                       tttCAACTCTGATGACAATAGCAGGTTTAGGAACACTGTCAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTc                                                                                         <  1:1010386/148‑1 (MQ=255)
                                                       tttCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTc                                                                                         <  1:2310524/148‑1 (MQ=255)
                                                         tCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCtg                                                                                       <  2:816929/148‑1 (MQ=255)
                                                           aaCTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCtgtg                                                                                     <  2:714446/148‑1 (MQ=255)
                                                                tgatgaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGaaaaa                                                                                <  1:1080834/148‑1 (MQ=255)
                                                                 gatgaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAAc                                                                               >  1:3161940/1‑148 (MQ=255)
                                                                  atgaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAAct                                                                              <  2:1361311/148‑1 (MQ=255)
                                                                  atgaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAAct                                                                              >  1:3138732/1‑148 (MQ=255)
                                                                    gaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActct                                                                            >  2:3013393/1‑148 (MQ=255)
                                                                    gaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGCCCTCTTTTTTTTATTTCTGTGAAAAActct                                                                            <  1:2118795/148‑1 (MQ=255)
                                                                      cAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttc                                                                          >  2:171424/1‑148 (MQ=255)
                                                                         tAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttctct                                                                       <  1:3241148/148‑1 (MQ=255)
                                                                         tAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTATGTGAATTAgtcttctct                                                                       >  2:2199977/1‑148 (MQ=255)
                                                                          aGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttctctt                                                                      <  2:1215498/148‑1 (MQ=255)
                                                                          aGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttctctt                                                                      <  2:109786/148‑1 (MQ=255)
                                                                           gCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTta                                                                     >  1:2407547/1‑148 (MQ=255)
                                                                             aGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTtata                                                                   <  2:438797/148‑1 (MQ=255)
                                                                                 ttAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGAt                                                               <  1:814242/148‑1 (MQ=255)
                                                                                   aGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTa                                                             <  1:3007846/148‑1 (MQ=255)
                                                                                     gAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATg                                                           >  1:3118438/1‑148 (MQ=255)
                                                                                      aaCACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGt                                                          >  1:1542323/1‑148 (MQ=255)
                                                                                       acacTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTATCTTATAGGATTATGtc                                                         >  2:2149266/1‑148 (MQ=255)
                                                                                       acacTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGtc                                                         <  2:589234/148‑1 (MQ=255)
                                                                                        cacTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGtct                                                        <  1:141437/148‑1 (MQ=255)
                                                                                        cacTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGtct                                                        <  1:1053150/148‑1 (MQ=255)
                                                                                        cacTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGtct                                                        <  1:2191500/148‑1 (MQ=255)
                                                                                         acTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGtctc                                                       >  2:3058295/1‑148 (MQ=255)
                                                                                          cTGACATGTACAATATTTTTTGTTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTctct                                                      <  2:1752485/148‑1 (MQ=255)
                                                                                          cTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTctct                                                      <  2:1969409/148‑1 (MQ=255)
                                                                                          cTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTctct                                                      <  1:1340185/148‑1 (MQ=255)
                                                                                          cTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTctct                                                      >  2:1891755/1‑148 (MQ=255)
                                                                                            gACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCttt                                                    <  2:2397312/148‑1 (MQ=255)
                                                                                             aCAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATTTCTCTTTg                                                   >  1:2426063/1‑148 (MQ=255)
                                                                                             aCAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTACTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTg                                                   >  2:1810199/1‑148 (MQ=255)
                                                                                              cAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTgg                                                  >  2:3253337/1‑148 (MQ=255)
                                                                                               aGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGa                                                 <  2:1924243/148‑1 (MQ=255)
                                                                                                ggTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGAt                                                >  2:1397414/1‑148 (MQ=255)
                                                                                                  tACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTa                                              <  1:71900/148‑1 (MQ=255)
                                                                                                  tACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTa                                              <  2:3202285/148‑1 (MQ=255)
                                                                                                      atatTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGt                                          <  1:258946/148‑1 (MQ=255)
                                                                                                      atatTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGt                                          >  1:787762/1‑148 (MQ=255)
                                                                                                        atTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGttt                                        >  1:1498887/1‑148 (MQ=255)
                                                                                                         ttttttGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTa                                       >  1:466436/1‑148 (MQ=255)
                                                                                                          tttttGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTAc                                      >  1:436329/1‑148 (MQ=255)
                                                                                                          tttttGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTAc                                      >  1:2420009/1‑148 (MQ=255)
                                                                                                          tttttGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTAc                                      <  2:1677755/148‑1 (MQ=255)
                                                                                                              tGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAAt                                  <  1:3037259/148‑1 (MQ=255)
                                                                                                                  tttGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAg                              >  1:2444110/1‑148 (MQ=255)
                                                                                                                     gttgGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTa                           <  1:393554/148‑1 (MQ=255)
                                                                                                                      ttgGTGATAAGATTGGTGTAAAAAAAAACTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTAt                          <  1:2489019/148‑1 (MQ=255)
                                                                                                                         gTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGc                       <  1:1958117/148‑1 (MQ=255)
                                                                                                                          tGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCa                      <  1:1014352/148‑1 (MQ=255)
                                                                                                                          tGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCa                      <  2:1868285/148‑1 (MQ=255)
                                                                                                                           gATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAg                     <  2:2647056/148‑1 (MQ=255)
                                                                                                                            aTAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAgg                    >  2:1357999/1‑148 (MQ=255)
                                                                                                                             tAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAggg                   >  2:2400999/1‑148 (MQ=255)
                                                                                                                             tAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAggg                   <  2:1644815/148‑1 (MQ=255)
                                                                                                                              aaGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggt                  <  1:592474/148‑1 (MQ=255)
                                                                                                                              aaGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggt                  >  1:741774/1‑148 (MQ=255)
                                                                                                                              aaGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggt                  >  1:1152009/1‑148 (MQ=255)
                                                                                                                              aaGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTGTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTGTGTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggt                  >  1:3300730/1‑148 (MQ=255)
                                                                                                                                  ttGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttggt              >  2:2352961/1‑148 (MQ=255)
                                                                                                                                      tgtAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGTTTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCa          >  1:307865/1‑148 (MQ=255)
                                                                                                                                       gtAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTTCAGGGTTGGTTCCaa         >  1:2084209/1‑148 (MQ=255)
                                                                                                                                        tAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCaaa        >  1:1814079/1‑148 (MQ=255)
                                                                                                                                        tAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCaaa        >  1:2623011/1‑148 (MQ=255)
                                                                                                                                            aaaaACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAAttt    >  1:714998/1‑148 (MQ=255)
                                                                                                                                             aaaaCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTta   >  1:879959/1‑148 (MQ=255)
                                                                                                                                             aaaaCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTta   >  1:2678175/1‑148 (MQ=255)
                                                                                                                                              aaGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTtat  >  1:541976/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
ATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTTAT  >  CP009273/4494577‑4494866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑