Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,495,560 A→T 13.3% N153Y (AAC→TAC)  yjhC → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,495,5600AT13.3% 649.0 / 75.9 225N153Y (AAC→TAC) yjhCputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (81/114);  new base T (17/13);  total (98/127)
Fisher's exact test for biased strand distribution p-value = 1.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 235 total aligned reads displayed.

AAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATTGGTGGAAATTTGGCCCATT  >  CP009273/4495417‑4495707
                                                                                                                                               |                                                                                                                                                   
 aGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAaaacaaa                                                                                                                                                >  1:2688999/1‑148 (MQ=255)
 aGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAaaacaaa                                                                                                                                                >  2:2377667/1‑148 (MQ=255)
       tAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAagag                                                                                                                                          <  2:2617314/148‑1 (MQ=255)
       tAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAagag                                                                                                                                          <  2:2454816/148‑1 (MQ=255)
       tAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAagag                                                                                                                                          <  2:1134458/148‑1 (MQ=255)
       tAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAagag                                                                                                                                          >  1:1957410/1‑148 (MQ=255)
       tAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTAAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAagag                                                                                                                                          <  1:524718/148‑1 (MQ=255)
          agaagCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAgagaga                                                                                                                                       <  1:1011197/148‑1 (MQ=255)
          agaagCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAgagaga                                                                                                                                       >  1:2370007/1‑148 (MQ=255)
          agaagCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAgagaga                                                                                                                                       >  2:1305924/1‑148 (MQ=255)
              gCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACttt                                                                                                                                   <  1:1633317/148‑1 (MQ=255)
                   tgtgACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTgg                                                                                                                              >  1:224340/1‑148 (MQ=255)
                       aCCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGaaaa                                                                                                                          >  2:551904/1‑148 (MQ=255)
                         cTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAg                                                                                                                        <  2:1812521/148‑1 (MQ=255)
                          tttATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGa                                                                                                                       <  1:2227394/148‑1 (MQ=255)
                          tttATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGa                                                                                                                       <  2:442809/148‑1 (MQ=255)
                             aTGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGa                                                                                                                    <  2:1254907/148‑1 (MQ=255)
                               ggCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGaaa                                                                                                                  <  2:1620790/148‑1 (MQ=255)
                                gCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAg                                                                                                                 <  1:915076/148‑1 (MQ=255)
                                gCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAg                                                                                                                 <  1:639006/148‑1 (MQ=255)
                                  cGGTCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGaa                                                                                                               <  1:1987264/148‑1 (MQ=255)
                                       atatTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATc                                                                                                          >  2:1023695/1‑148 (MQ=255)
                                        tattatGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCt                                                                                                         <  2:1490973/148‑1 (MQ=255)
                                        tattatGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCt                                                                                                         >  2:7315/1‑148 (MQ=255)
                                        tattatGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGAGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCt                                                                                                         >  2:1901214/1‑148 (MQ=255)
                                         attatGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCtg                                                                                                        >  2:1363806/1‑148 (MQ=255)
                                         attatGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCtg                                                                                                        <  2:2504092/148‑1 (MQ=255)
                                           tatGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCtggt                                                                                                      >  2:226793/1‑148 (MQ=255)
                                            atGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCtggtg                                                                                                     >  1:809265/1‑148 (MQ=255)
                                             tGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCtggtgg                                                                                                    >  1:1756120/1‑148 (MQ=255)
                                              gAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGa                                                                                                   <  1:66235/148‑1 (MQ=255)
                                              gAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGa                                                                                                   <  1:596041/148‑1 (MQ=255)
                                              gAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGa                                                                                                   >  2:1399533/1‑148 (MQ=255)
                                              gAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGa                                                                                                   <  1:3312688/148‑1 (MQ=255)
                                                 ttttttCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAATAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACAt                                                                                                >  2:1279456/1‑148 (MQ=255)
                                                 ttttttCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACAt                                                                                                <  2:3295924/148‑1 (MQ=255)
                                                       cAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTatat                                                                                          <  1:622860/148‑1 (MQ=255)
                                                       cAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTatat                                                                                          <  2:100728/148‑1 (MQ=255)
                                                          tGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATatcat                                                                                       <  1:1743654/148‑1 (MQ=255)
                                                          tGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATatcat                                                                                       >  2:533767/1‑148 (MQ=255)
                                                             ggTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATcatcat                                                                                    <  2:1686160/148‑1 (MQ=255)
                                                             ggTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATcatcat                                                                                    >  1:2470251/1‑148 (MQ=255)
                                                              gTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCata                                                                                   <  1:604785/148‑1 (MQ=255)
                                                              gTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCata                                                                                   <  1:2803171/148‑1 (MQ=255)
                                                               ttCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCatat                                                                                  >  1:2303048/1‑148 (MQ=255)
                                                                 cAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATAc                                                                                <  2:2719351/148‑1 (MQ=255)
                                                                 cAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATAc                                                                                <  1:1045790/148‑1 (MQ=255)
                                                                      tGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAg                                                                           <  2:886958/148‑1 (MQ=255)
                                                                          cGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTTGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAg                                                                       <  2:2828021/148‑1 (MQ=255)
                                                                          cGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAg                                                                       <  1:1215355/148‑1 (MQ=255)
                                                                            gAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGAt                                                                     <  1:2129518/148‑1 (MQ=255)
                                                                             aaGTTAATTAAAGAAGGTGTTCTCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGAtt                                                                    <  2:1761387/148‑1 (MQ=255)
                                                                               gTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATtgt                                                                  >  1:2553917/1‑148 (MQ=255)
                                                                               gTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATtgt                                                                  >  2:182655/1‑148 (MQ=255)
                                                                                ttaattaaAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATtgtg                                                                 <  1:2621966/148‑1 (MQ=255)
                                                                                 taattaaAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTgtgt                                                                >  1:2488958/1‑148 (MQ=255)
                                                                                 taattaaAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTgtgt                                                                <  1:454641/148‑1 (MQ=255)
                                                                                  aattaaAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGtt                                                               <  2:3089814/148‑1 (MQ=255)
                                                                                  aattaaAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGtt                                                               <  2:960150/148‑1 (MQ=255)
                                                                                    ttaaAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCa                                                             >  1:134263/1‑148 (MQ=255)
                                                                                    ttaaAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCa                                                             >  2:87882/1‑148 (MQ=255)
                                                                                        agaagGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCAt                                                         <  1:267923/148‑1 (MQ=255)
                                                                                        agaagGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCAt                                                         >  2:488265/1‑148 (MQ=255)
                                                                                           agGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTa                                                      >  2:3243805/1‑148 (MQ=255)
                                                                                             gtgtTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACt                                                    <  1:1220165/148‑1 (MQ=255)
                                                                                              tgtTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACtt                                                   <  1:1486785/148‑1 (MQ=255)
                                                                                               gtTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTg                                                  >  2:2771295/1‑148 (MQ=255)
                                                                                                ttATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTgg                                                 >  2:2692305/1‑148 (MQ=255)
                                                                                                 tATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGga                                                >  1:1498243/1‑148 (MQ=255)
                                                                                                  aTCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACACGAGAGACTTTCCTGGAAAAAGATGAAAGAACACTCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGgag                                               <  1:2377667/148‑1 (MQ=255)
                                                                                                  aTCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGgag                                               >  2:1983636/1‑148 (MQ=255)
                                                                                                    cGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGaa                                             <  2:2905325/148‑1 (MQ=255)
                                                                                                      gCGAAATATTATCATGTCATACTAAGAGCAAGGGCTGGGAAAACAAACCAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAAt                                           <  2:2023454/148‑1 (MQ=255)
                                                                                                      gCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAAt                                           <  2:3147294/148‑1 (MQ=255)
                                                                                                       cGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATa                                          <  1:3051045/148‑1 (MQ=255)
                                                                                                          aaTATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCa                                       <  2:651157/148‑1 (MQ=255)
                                                                                                           atatTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCag                                      >  1:1452927/1‑148 (MQ=255)
                                                                                                              ttatCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAgaga                                   <  1:2234503/148‑1 (MQ=255)
                                                                                                                 tCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACgg                                <  1:182655/148‑1 (MQ=255)
                                                                                                                 tCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACgg                                <  1:3175439/148‑1 (MQ=255)
                                                                                                                    tGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATAACAGAGACGGTTa                             >  1:888891/1‑148 (MQ=255)
                                                                                                                     gTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTAc                            >  2:2221184/1‑148 (MQ=255)
                                                                                                                     gTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTAc                            >  1:59721/1‑148 (MQ=255)
                                                                                                                     gTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTAc                            >  1:3278695/1‑148 (MQ=255)
                                                                                                                      tCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACt                           <  1:1023695/148‑1 (MQ=255)
                                                                                                                      tCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACt                           <  1:2879761/148‑1 (MQ=255)
                                                                                                                         tACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATg                        <  2:2370007/148‑1 (MQ=255)
                                                                                                                           cTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGAt                      <  2:263203/148‑1 (MQ=255)
                                                                                                                               gagaAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATtggt                  <  2:976021/148‑1 (MQ=255)
                                                                                                                                  aaaTGGCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATTGGTGGa               >  2:1302280/1‑148 (MQ=255)
                                                                                                                                   aaTGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATTGGTGGaa              >  1:44030/1‑148 (MQ=255)
                                                                                                                                      ggCTGGGAATACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATTGGTGGAAAtt           >  2:159267/1‑148 (MQ=255)
                                                                                                                                      ggCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATTGGTGGAAAtt           <  1:2404925/148‑1 (MQ=255)
                                                                                                                                       gCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATTGGTGGAAAttt          >  2:1518471/1‑148 (MQ=255)
                                                                                                                                         tGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATTGGTGGAAATTTgg        <  2:1171571/148‑1 (MQ=255)
                                                                                                                                           ggAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATTGGTGGAAATTTGGcc      <  1:502062/148‑1 (MQ=255)
                                                                                                                                           ggAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATTGGTGGAAATTTGGcc      >  1:1843113/1‑148 (MQ=255)
                                                                                                                                           ggAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATTGGTGGAAATTTGGcc      >  1:179226/1‑148 (MQ=255)
                                                                                                                                            gAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATTGGTGGAAATTTGGccc     >  2:475034/1‑148 (MQ=255)
                                                                                                                                              aaacaaacAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATTGGTGGAAATTTGGCCCAt   <  1:2185501/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
AAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGTTAATTAAAGAAGGTGTTATCGGCGAAATATTATCATGTCATACTAAGAGAAATGGCTGGGAAAACAAACAAGAGAGACTTTCCTGGAAAAAGATGAAAGAACAATCTGGTGGACATCTATATCATCATATACATGAGTTAGATTGTGTTCAGCATTTACTTGGAGAAATACCAGAGACGGTTACTATGATTGGTGGAAATTTGGCCCATT  >  CP009273/4495417‑4495707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑