Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 372,091 C→A 93.3% R393R (CGG→AGG)  mhpT → 3‑hydroxyphenylpropionic transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273372,0910CA93.3% 369.1 / 19.7 134R393R (CGG→AGG) mhpT3‑hydroxyphenylpropionic transporter
Reads supporting (aligned to +/- strand):  ref base C (3/6);  new base A (71/54);  total (74/60)
Fisher's exact test for biased strand distribution p-value = 2.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

Only 100 of 134 total aligned reads displayed.

CGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAA  >  CP009273/371946‑372235
                                                                                                                                                 |                                                                                                                                                
cGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAgg                                                                                                                                                <  1:1981458/148‑1 (MQ=255)
        tAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCGTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACg                                                                                                                                        >  1:2565608/1‑148 (MQ=255)
          gggCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGaa                                                                                                                                      >  1:3134844/1‑148 (MQ=255)
           ggCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAAt                                                                                                                                     <  2:2090669/148‑1 (MQ=255)
            gCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATa                                                                                                                                    <  1:1391310/148‑1 (MQ=255)
            gCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATa                                                                                                                                    >  1:2458912/1‑148 (MQ=255)
             cGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATAc                                                                                                                                   <  1:78648/148‑1 (MQ=255)
             cGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATAc                                                                                                                                   <  1:80035/148‑1 (MQ=255)
             cGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATAc                                                                                                                                   <  2:2965688/148‑1 (MQ=255)
                 tGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACTGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGcc                                                                                                                               <  1:2369128/148‑1 (MQ=255)
                 tGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGcc                                                                                                                               <  1:2369127/148‑1 (MQ=255)
                  gggggCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCg                                                                                                                              >  1:2448680/1‑148 (MQ=255)
                    gggCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTg                                                                                                                            >  2:1619449/1‑148 (MQ=255)
                      gCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTgcg                                                                                                                          >  1:2995898/1‑148 (MQ=255)
                             gCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGc                                                                                                                   <  2:1821041/148‑1 (MQ=255)
                                gTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGccgg                                                                                                                >  1:24236/1‑146 (MQ=255)
                                gTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTg                                                                                                                <  1:2829009/148‑1 (MQ=255)
                                 tCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGa                                                                                                               >  2:3176614/1‑148 (MQ=255)
                                   cGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACg                                                                                                             >  2:3285139/1‑148 (MQ=255)
                                   cGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACg                                                                                                             >  2:1059493/1‑148 (MQ=255)
                                       aCTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGcc                                                                                                         >  2:280343/1‑148 (MQ=255)
                                         tGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCtt                                                                                                       <  2:194994/148‑1 (MQ=255)
                                           gCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCttat                                                                                                     >  1:2398473/1‑148 (MQ=255)
                                           gCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCGGACGTGCCttat                                                                                                     >  1:134119/1‑148 (MQ=255)
                                                gAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGc                                                                                                >  2:3113379/1‑148 (MQ=255)
                                                 aaaaTGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCa                                                                                               <  2:2551279/148‑1 (MQ=255)
                                                 aaaaTGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGGTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCa                                                                                               <  2:2551287/148‑1 (MQ=255)
                                                   aaTGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAgg                                                                                             >  2:1701383/1‑148 (MQ=255)
                                                   aaTGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTAGTAGGCAgg                                                                                             >  2:1701374/1‑148 (MQ=255)
                                                     tGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAgggg                                                                                           <  2:505156/148‑1 (MQ=255)
                                                       cTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCg                                                                                         >  2:1893105/1‑148 (MQ=255)
                                                         ggCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGaa                                                                                       <  2:820848/148‑1 (MQ=255)
                                                          gCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGaaa                                                                                      <  1:915548/148‑1 (MQ=255)
                                                          gCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGaaa                                                                                      <  1:2527858/148‑1 (MQ=255)
                                                          gCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGaaa                                                                                      <  1:915008/148‑1 (MQ=255)
                                                           cATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAg                                                                                     >  2:1375616/1‑148 (MQ=255)
                                                             ttAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAggg                                                                                   >  2:1230312/1‑148 (MQ=255)
                                                             ttAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAggg                                                                                   >  1:2640767/1‑148 (MQ=255)
                                                             ttAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAggg                                                                                   >  1:799089/1‑148 (MQ=255)
                                                              tAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGa                                                                                  >  2:2728664/1‑148 (MQ=255)
                                                              tAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCGTATTAGGCAGGGGCGAAAGGGa                                                                                  >  2:2728666/1‑148 (MQ=255)
                                                                ggCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACt                                                                                >  2:1009158/1‑148 (MQ=255)
                                                                ggCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACt                                                                                >  1:1201155/1‑148 (MQ=255)
                                                                ggCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACt                                                                                <  1:1667850/148‑1 (MQ=255)
                                                                 gCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCGGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACtt                                                                               >  1:1709858/1‑148 (MQ=255)
                                                                  cACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTc                                                                              <  1:2429550/148‑1 (MQ=255)
                                                                  cACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTc                                                                              <  1:2429937/148‑1 (MQ=255)
                                                                    cTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTGATTAGGCAGGGGCGAAAGGGACTTCAc                                                                            >  2:2640564/1‑148 (MQ=255)
                                                                     tGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCAcc                                                                           >  1:2917634/1‑148 (MQ=255)
                                                                        cACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCa                                                                        <  1:1467394/148‑1 (MQ=255)
                                                                            gTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGACGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATac                                                                    >  2:1496105/1‑148 (MQ=255)
                                                                                 cGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAACGGGACTTCACCCCAATACACCTa                                                               >  2:1867971/1‑148 (MQ=255)
                                                                                 cGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTa                                                               >  2:318570/1‑148 (MQ=255)
                                                                                 cGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTa                                                               >  2:319296/1‑148 (MQ=255)
                                                                                   tAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACg                                                             >  1:1963246/1‑148 (MQ=255)
                                                                                     aTGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGc                                                           >  2:1899959/1‑148 (MQ=255)
                                                                                      tGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCg                                                          <  1:1334927/148‑1 (MQ=255)
                                                                                       ggCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGt                                                         <  1:2052620/148‑1 (MQ=255)
                                                                                              tCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAgg                                                  >  2:2931092/1‑148 (MQ=255)
                                                                                                      gggTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCNCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAACGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCg                                          >  1:509750/1‑148 (MQ=255)
                                                                                                      gggTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCg                                          <  1:1987160/148‑1 (MQ=255)
                                                                                                       ggTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGc                                         <  2:423649/148‑1 (MQ=255)
                                                                                                           ttCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTaaa                                     >  1:2381702/1‑148 (MQ=255)
                                                                                                           ttCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTaaa                                     >  1:2382279/1‑148 (MQ=255)
                                                                                                           ttCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTaaa                                     >  1:2382302/1‑148 (MQ=255)
                                                                                                           ttCTTGGTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTaaa                                     <  2:1467878/148‑1 (MQ=255)
                                                                                                            tCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTaaaa                                    >  2:2415966/1‑148 (MQ=255)
                                                                                                            tCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTaaaa                                    >  2:1946760/1‑148 (MQ=255)
                                                                                                            tCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTaaaa                                    >  1:3139937/1‑148 (MQ=255)
                                                                                                              ttgttgCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAat                                  <  2:827138/148‑1 (MQ=255)
                                                                                                               tgttgCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAatt                                 <  2:2565608/148‑1 (MQ=255)
                                                                                                                gttgCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAatta                                <  1:1622427/148‑1 (MQ=255)
                                                                                                                 ttgCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAattat                               <  2:40150/148‑1 (MQ=255)
                                                                                                                 ttgCGGGGGTGGCGGTGTTTCTGTTGATGCGCAGGAGATCACGACTACAGCCGTGCGCCGATGCCTGACTTGCCTTATTAGGCAGGGACGAAAGGGACTTCACCGCAATACAGCTACGGCGTTTACAGGTATACTCGCTAAAAattat                               <  1:1991212/148‑1 (MQ=255)
                                                                                                                  tgCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAattatt                              >  2:2017984/1‑148 (MQ=255)
                                                                                                                  tgCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAattatt                              >  1:2531885/1‑148 (MQ=255)
                                                                                                                    cTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCa                            <  2:3047315/148‑1 (MQ=255)
                                                                                                                       ggTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCg                         >  1:1297263/1‑148 (MQ=255)
                                                                                                                         ttGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCggg                       <  1:1479675/148‑1 (MQ=255)
                                                                                                                            gCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGttt                    <  1:448881/148‑1 (MQ=255)
                                                                                                                               gtgtTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGa                 >  2:2177299/1‑148 (MQ=255)
                                                                                                                                tgtTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGaa                >  1:1468326/1‑148 (MQ=255)
                                                                                                                                tgtTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGaa                >  1:1468348/1‑148 (MQ=255)
                                                                                                                                tgtTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGaa                >  1:2753876/1‑148 (MQ=255)
                                                                                                                                  tttATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAAc              >  1:407254/1‑148 (MQ=255)
                                                                                                                                   ttATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACa             <  1:1059493/148‑1 (MQ=255)
                                                                                                                                   ttATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACa             <  2:1679629/148‑1 (MQ=255)
                                                                                                                                    tATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACaa            <  1:3872/148‑1 (MQ=255)
                                                                                                                                    tATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACaa            <  1:292739/148‑1 (MQ=255)
                                                                                                                                     aTTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACaaa           <  1:3153565/148‑1 (MQ=255)
                                                                                                                                      ttttGATGAGCAGGAGATTACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAg          <  2:1012386/148‑1 (MQ=255)
                                                                                                                                      ttttGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAg          >  1:362665/1‑148 (MQ=255)
                                                                                                                                      ttttGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAg          >  1:2240282/1‑148 (MQ=255)
                                                                                                                                      ttttGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAg          >  1:2028226/1‑148 (MQ=255)
                                                                                                                                       tttGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGa         >  1:1805066/1‑148 (MQ=255)
                                                                                                                                           atgaGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGc     >  1:930151/1‑148 (MQ=255)
                                                                                                                                           atgaGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGc     >  2:328979/1‑148 (MQ=255)
                                                                                                                                           atgaGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGc     >  2:328990/1‑148 (MQ=255)
                                                                                                                                             gaGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTAGTAGGCAGGGGCGAAAGGGTCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGACACAAAGATGGCaa   >  1:1824814/1‑148 (MQ=255)
                                                                                                                                              aGCAGGAGATCACGAATACAGCCGTGCGCCGAGGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCaaa  <  2:2995898/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAA  >  CP009273/371946‑372235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑