BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,495,859	T→G	90.0%	D252E (GAT→GAG)	yjhC →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,495,859	0	T	G	90.0%	653.0 / 31.8	189	D252E (GAT→GAG)	yjhC	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (12/7); new base G (83/87); total (95/94)
Fisher's exact test for biased strand distribution p-value = 3.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 189 total aligned reads displayed.

AGGATTTGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAG  >  CP009273/4495715‑4496001

aGGATTTGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATg                                                                                                                                             <  2:806353/148‑1 (MQ=255)

    tttGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAg                                                                                                                                         >  1:1520882/1‑148 (MQ=255)

    tttGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAg                                                                                                                                         >  2:760720/1‑148 (MQ=255)

     ttGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGa                                                                                                                                        >  2:2405128/1‑148 (MQ=255)

     ttGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGAAAGa                                                                                                                                        >  2:2245209/1‑148 (MQ=255)

       ggCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGaaa                                                                                                                                      <  1:2064180/148‑1 (MQ=255)

        gCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAAc                                                                                                                                     <  1:2165893/148‑1 (MQ=255)

        gCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAAc                                                                                                                                     <  1:1789774/148‑1 (MQ=255)

        gCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAAc                                                                                                                                     <  2:1184204/148‑1 (MQ=255)

         cAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAAca                                                                                                                                    >  1:2542677/1‑148 (MQ=255)

           aTGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAAcagc                                                                                                                                  <  1:308406/148‑1 (MQ=255)

                gatgatATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGt                                                                                                                             >  2:275917/1‑148 (MQ=255)

                gatgatATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGt                                                                                                                             >  1:1847778/1‑148 (MQ=255)

                  tgatATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCa                                                                                                                           >  2:533580/1‑148 (MQ=255)

                  tgatATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCa                                                                                                                           <  1:488242/148‑1 (MQ=255)

                  tgatATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCa                                                                                                                           >  2:125429/1‑148 (MQ=255)

                     tatGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCAcct                                                                                                                        >  1:2944202/1‑146 (MQ=255)

                       tGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAg                                                                                                                      <  1:1518471/148‑1 (MQ=255)

                        gTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAgg                                                                                                                     >  2:285525/1‑148 (MQ=255)

                          tatttatGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGAt                                                                                                                   <  1:991975/148‑1 (MQ=255)

                           atttatGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGAtt                                                                                                                  <  1:1514522/148‑1 (MQ=255)

                            tttatGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTg                                                                                                                 <  2:865367/148‑1 (MQ=255)

                                tGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCgg                                                                                                             <  2:1856301/148‑1 (MQ=255)

                                 gACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGt                                                                                                            >  1:2464242/1‑148 (MQ=255)

                                 gACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGt                                                                                                            <  2:633460/148‑1 (MQ=255)

                                  aCCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTc                                                                                                           >  2:3095657/1‑148 (MQ=255)

                                  aCCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTc                                                                                                           >  1:669539/1‑148 (MQ=255)

                                    cTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAg                                                                                                         >  1:2786541/1‑148 (MQ=255)

                                     ttGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGa                                                                                                        <  1:659326/148‑1 (MQ=255)

                                     ttGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGAGGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGa                                                                                                        <  1:2320852/148‑1 (MQ=255)

                                      tGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGAc                                                                                                       >  2:3117544/1‑148 (MQ=255)

                                      tGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGAc                                                                                                       >  2:3117520/1‑148 (MQ=255)

                                                gTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCAtttt                                                                                             <  2:2787810/148‑1 (MQ=255)

                                                 tCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCAttttt                                                                                            >  2:3096666/1‑148 (MQ=255)

                                                  cAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCAtttttt                                                                                           <  1:801364/148‑1 (MQ=255)

                                                    ggAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTgg                                                                                         >  2:315007/1‑148 (MQ=255)

                                                    ggAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTgg                                                                                         >  2:1401218/1‑148 (MQ=255)

                                                     gAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGt                                                                                        >  2:529981/1‑148 (MQ=255)

                                                      aaaaCTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTc                                                                                       >  1:1885918/1‑148 (MQ=255)

                                                      aaaaCTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTc                                                                                       <  1:2909667/148‑1 (MQ=255)

                                                         aCTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCAt                                                                                    <  2:209210/148‑1 (MQ=255)

                                                         aCTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCAt                                                                                    >  1:2442317/1‑148 (MQ=255)

                                                         aCTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCAt                                                                                    >  1:1313553/1‑148 (MQ=255)

                                                            aGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGaa                                                                                 >  2:173627/1‑148 (MQ=255)

                                                            aGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGaa                                                                                 >  1:1336261/1‑148 (MQ=255)

                                                             gCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGaaa                                                                                <  2:3246385/148‑1 (MQ=255)

                                                             gCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGAAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGaaa                                                                                >  1:631108/1‑148 (MQ=255)

                                                                aCCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAacac                                                                             >  1:2202301/1‑148 (MQ=255)

                                                                 ccTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAAcaca                                                                            <  2:2669309/148‑1 (MQ=255)

                                                                  cTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACaa                                                                           >  2:207627/1‑148 (MQ=255)

                                                                  cTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACaa                                                                           >  2:206299/1‑148 (MQ=255)

                                                                   ttAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACACAGCATTTTTTGGTCCATGAAACACaag                                                                          >  1:108159/1‑148 (MQ=255)

                                                                    tAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACaaga                                                                         >  1:2050929/1‑148 (MQ=255)

                                                                    tAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACaaga                                                                         >  2:828451/1‑148 (MQ=255)

                                                                    tAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACaaga                                                                         <  1:125429/148‑1 (MQ=255)

                                                                      gagTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACaagaag                                                                       <  1:879521/148‑1 (MQ=255)

                                                                        gTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACaagaagaa                                                                     <  1:707553/148‑1 (MQ=255)

                                                                            gggAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAgatg                                                                 >  1:3312713/1‑148 (MQ=255)

                                                                             tgAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAAATAAAGGCTATATTAAAATTGAGATGCATGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAgatga                                                                <  1:2245209/147‑1 (MQ=255)

                                                                               aGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAgatgatg                                                              >  2:2709214/1‑148 (MQ=255)

                                                                                gTGGATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAgatgatga                                                             <  2:377962/148‑1 (MQ=255)

                                                                                  gCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGAtc                                                           >  1:70037/1‑148 (MQ=255)

                                                                                   cATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGAtcg                                                          >  1:2433612/1‑148 (MQ=255)

                                                                                         aCTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGaa                                                    >  1:209929/1‑148 (MQ=255)

                                                                                            ggCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAgg                                                 <  2:2898052/148‑1 (MQ=255)

                                                                                              ccGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCa                                               <  1:1109591/148‑1 (MQ=255)

                                                                                                    cATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGa                                         >  2:2435879/1‑148 (MQ=255)

                                                                                                    cATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGa                                         >  2:778262/1‑148 (MQ=255)

                                                                                                       tATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCt                                      <  2:3295156/148‑1 (MQ=255)

                                                                                                       tATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCt                                      <  2:2149301/148‑1 (MQ=255)

                                                                                                        aTGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTc                                     <  1:1924541/148‑1 (MQ=255)

                                                                                                         tGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCa                                    >  1:3123434/1‑148 (MQ=255)

                                                                                                            catcatcaATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGaa                                 <  1:1134827/148‑1 (MQ=255)

                                                                                                             atcatcaATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGaaa                                <  2:963908/148‑1 (MQ=255)

                                                                                                                atcaATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAatgg                             <  2:35086/148‑1 (MQ=255)

                                                                                                                  caATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAatggat                           <  2:356692/148‑1 (MQ=255)

                                                                                                                  caATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAatggat                           <  2:536852/148‑1 (MQ=255)

                                                                                                                    aTGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAatggatgg                         >  1:449454/1‑148 (MQ=255)

                                                                                                                    aTGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAatggatgg                         <  1:2346859/148‑1 (MQ=255)

                                                                                                                    aTGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAatggatgg                         <  1:2346935/148‑1 (MQ=255)

                                                                                                                    aTGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAatggatgg                         <  1:549663/148‑1 (MQ=255)

                                                                                                                    aTGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAatggatgg                         <  2:1097340/148‑1 (MQ=255)

                                                                                                                       gAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGgcgc                      >  2:611477/1‑148 (MQ=255)

                                                                                                                       gAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGgcgc                      >  1:218006/1‑148 (MQ=255)

                                                                                                                        aaCTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCt                     >  1:2727171/1‑148 (MQ=255)

                                                                                                                          cTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCtat                   <  2:1130603/148‑1 (MQ=255)

                                                                                                                           tAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCtata                  >  1:2672393/1‑148 (MQ=255)

                                                                                                                             aaGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGc                <  1:2925082/148‑1 (MQ=255)

                                                                                                                             aaGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGc                <  1:496078/148‑1 (MQ=255)

                                                                                                                              aGGCTCTATTAAAATTGAGATGCAAGACACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCa               <  1:1587250/148‑1 (MQ=255)

                                                                                                                               ggCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCat              <  1:1357026/148‑1 (MQ=255)

                                                                                                                                gCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCata             >  2:2851044/1‑148 (MQ=255)

                                                                                                                                gCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCata             >  2:1268514/1‑148 (MQ=255)

                                                                                                                                gCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACCTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCata             <  2:2944202/148‑1 (MQ=255)

                                                                                                                                 ctctATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCatat            >  1:3197602/1‑148 (MQ=255)

                                                                                                                                  tctATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATg           >  1:136918/1‑148 (MQ=255)

                                                                                                                                     aTTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTc        <  1:386467/148‑1 (MQ=255)

                                                                                                                                       tAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCAt      <  2:2567459/148‑1 (MQ=255)

                                                                                                                                       tAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCAt      <  2:1560705/148‑1 (MQ=255)

                                                                                                                                          aaTTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCa   <  2:3029240/148‑1 (MQ=255)

AGGATTTGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAG  >  CP009273/4495715‑4496001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`