Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,545,107 G→T 10.7% D239Y (GAT→TAT)  uxuR → fructuronate‑inducible hexuronate regulon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,545,1070GT10.7% 695.3 / 38.1 207D239Y (GAT→TAT) uxuRfructuronate‑inducible hexuronate regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base G (96/88);  new base T (8/15);  total (104/103)
Fisher's exact test for biased strand distribution p-value = 1.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

GACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTATTACTTTGTAAATTGCAGGG  >  CP009273/4544960‑4545253
                                                                                                                                                   |                                                                                                                                                  
gACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACg                                                                                                                                                    >  1:286276/1‑148 (MQ=255)
gACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACg                                                                                                                                                    <  1:551737/148‑1 (MQ=255)
gACAGCCTGTAGCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACg                                                                                                                                                    <  2:1772379/148‑1 (MQ=255)
   aGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGata                                                                                                                                                 >  1:2366448/1‑148 (MQ=255)
     ccTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACTAtatt                                                                                                                                               >  1:166707/1‑148 (MQ=255)
      cTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATTCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGAgattt                                                                                                                                              >  1:569360/1‑148 (MQ=255)
      cTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGAtattt                                                                                                                                              >  1:436807/1‑148 (MQ=255)
        gTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGTAACGTCTGCTGGAATTCTCGAACGTTGACGAtatttat                                                                                                                                            >  2:2661126/1‑148 (MQ=255)
        gTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGAtatttat                                                                                                                                            >  1:1467301/1‑148 (MQ=255)
         tATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGAtatttatt                                                                                                                                           <  1:3000652/148‑1 (MQ=255)
          aTCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACTATatttattt                                                                                                                                          <  1:2274469/148‑1 (MQ=255)
          aTCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATatttattt                                                                                                                                          <  2:1641768/148‑1 (MQ=255)
              cAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGAt                                                                                                                                      <  2:2050201/148‑1 (MQ=255)
              cAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGAt                                                                                                                                      <  2:2832706/148‑1 (MQ=255)
                 agagTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGc                                                                                                                                   >  1:1904269/1‑148 (MQ=255)
                  gagTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCt                                                                                                                                  >  1:805029/1‑148 (MQ=255)
                    gTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTAt                                                                                                                                <  1:2695423/148‑1 (MQ=255)
                      ggTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCt                                                                                                                              <  2:286276/148‑1 (MQ=255)
                       gTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTg                                                                                                                             >  1:2185612/1‑148 (MQ=255)
                       gTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTg                                                                                                                             <  1:2942851/148‑1 (MQ=255)
                        ttGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGt                                                                                                                            >  2:2792650/1‑148 (MQ=255)
                           ggCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTg                                                                                                                         <  2:1039470/148‑1 (MQ=255)
                           ggCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTg                                                                                                                         >  1:2858871/1‑148 (MQ=255)
                             cGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGAt                                                                                                                       >  1:2043844/1‑148 (MQ=255)
                                tCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACTATATTTATTTTGATGGCTATCTGTTTGATTCa                                                                                                                    <  2:854300/148‑1 (MQ=255)
                                 cacaAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCAt                                                                                                                   <  1:2678468/148‑1 (MQ=255)
                                    aaaCAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGc                                                                                                                >  2:893832/1‑148 (MQ=255)
                                     aaCAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGcc                                                                                                               >  1:154939/1‑148 (MQ=255)
                                      aCAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCg                                                                                                              >  2:411970/1‑148 (MQ=255)
                                          aTCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCTGCTgg                                                                                                          >  1:2667617/1‑148 (MQ=255)
                                            ccTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGAt                                                                                                        >  1:781097/1‑148 (MQ=255)
                                             cTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATa                                                                                                       <  2:49358/148‑1 (MQ=255)
                                              tCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATaa                                                                                                      <  1:625130/148‑1 (MQ=255)
                                               cgccgcGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATaaa                                                                                                     <  1:2226826/148‑1 (MQ=255)
                                               cgccgcGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATaaa                                                                                                     <  1:1389916/148‑1 (MQ=255)
                                                gccgcGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACTATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAg                                                                                                    <  2:1077939/148‑1 (MQ=255)
                                                gccgcGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAg                                                                                                    <  2:1546181/148‑1 (MQ=255)
                                                 ccgcGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGt                                                                                                   >  2:1996673/1‑148 (MQ=255)
                                                 ccgcGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGt                                                                                                   >  1:564030/1‑148 (MQ=255)
                                                   gcgTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCg                                                                                                 <  2:1317121/148‑1 (MQ=255)
                                                   gcgTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCg                                                                                                 >  1:1899354/1‑148 (MQ=255)
                                                    cgTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGa                                                                                                >  2:3181691/1‑148 (MQ=255)
                                                          tCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACTATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTg                                                                                          >  2:2249852/1‑148 (MQ=255)
                                                           cAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGa                                                                                         <  2:2584379/148‑1 (MQ=255)
                                                               aaaGATGCCCGAGCGGCGATGCTGGCAATGTGGCAGCTTCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACtta                                                                                     <  2:2438604/148‑1 (MQ=255)
                                                               aaaGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACtta                                                                                     >  1:2249637/1‑148 (MQ=255)
                                                               aaaGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACtta                                                                                     <  1:716244/148‑1 (MQ=255)
                                                               aaaGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACtta                                                                                     >  2:2677987/1‑148 (MQ=255)
                                                                aaGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACttat                                                                                    >  1:260063/1‑148 (MQ=255)
                                                                 aGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACttatt                                                                                   <  1:1966811/148‑1 (MQ=255)
                                                                     gCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTataa                                                                               >  2:3109940/1‑148 (MQ=255)
                                                                       ccGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACTATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTataata                                                                             <  2:2356644/148‑1 (MQ=255)
                                                                       ccGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTataata                                                                             >  2:289308/1‑148 (MQ=255)
                                                                        cGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTAtaataa                                                                            <  2:1167672/148‑1 (MQ=255)
                                                                        cGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTAtaataa                                                                            <  1:597667/148‑1 (MQ=255)
                                                                        cGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTAtaataa                                                                            <  1:3294517/148‑1 (MQ=255)
                                                                        cGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTAtaataa                                                                            <  1:3294165/148‑1 (MQ=255)
                                                                         gAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAg                                                                           >  2:299579/1‑148 (MQ=255)
                                                                                   gCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTa                                                                 >  2:1239230/1‑148 (MQ=255)
                                                                                       gCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATAATAATAAGCGCAAGGGTAAACg                                                             >  2:687516/1‑148 (MQ=255)
                                                                                         aaTGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGtt                                                           <  2:319226/148‑1 (MQ=255)
                                                                                         aaTGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGtt                                                           <  2:511136/148‑1 (MQ=255)
                                                                                         aaTGTGGCAGCATCTGGAAAACGTTAAGCAAAGTCTGCTGGAATTCTCGAACGTTTACGATATTTATTTTTATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGtt                                                           <  2:512384/148‑1 (MQ=255)
                                                                                          aTGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTc                                                          <  2:1067317/148‑1 (MQ=255)
                                                                                              ggCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTg                                                      >  1:3021615/1‑148 (MQ=255)
                                                                                                cagcaTCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGAAAGGGTAAACGTTCCTTgcg                                                    >  2:2919355/1‑148 (MQ=255)
                                                                                                   caTCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCtt                                                 <  1:308750/148‑1 (MQ=255)
                                                                                                     tCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTc                                               <  1:1107784/148‑1 (MQ=255)
                                                                                                          aaaaCGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACTATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTaaa                                          <  2:179033/148‑1 (MQ=255)
                                                                                                           aaaCGTTAAGCAACGTCTGCTTGAATTCTCGAACGTTGACTATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAAt                                         <  2:1714601/148‑1 (MQ=255)
                                                                                                            aaCGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACTATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAAtt                                        >  2:1365523/1‑148 (MQ=255)
                                                                                                            aaCGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACTATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAAtt                                        >  2:1365470/1‑148 (MQ=255)
                                                                                                            aaCGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAAtt                                        >  2:3144535/1‑148 (MQ=255)
                                                                                                            aaCGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAAtt                                        >  2:2417911/1‑148 (MQ=255)
                                                                                                            aaCGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAAtt                                        >  2:272791/1‑148 (MQ=255)
                                                                                                              cGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTaa                                      >  2:530891/1‑148 (MQ=255)
                                                                                                                 tAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTaagaa                                   >  2:1071259/1‑148 (MQ=255)
                                                                                                                   aGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGt                                 >  2:3307379/1‑148 (MQ=255)
                                                                                                                   aGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGt                                 <  2:2401747/148‑1 (MQ=255)
                                                                                                                    gCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTc                                >  1:2373787/1‑148 (MQ=255)
                                                                                                                       aCGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCa                             <  2:742254/148‑1 (MQ=255)
                                                                                                                           ctgctgGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGa                         >  1:1293121/1‑148 (MQ=255)
                                                                                                                           ctgctgGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGa                         <  2:1091610/148‑1 (MQ=255)
                                                                                                                           ctgctgGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGa                         >  1:3187384/1‑148 (MQ=255)
                                                                                                                             gctgGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGt                       <  1:3181691/148‑1 (MQ=255)
                                                                                                                              ctgGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTa                      <  2:3294771/148‑1 (MQ=255)
                                                                                                                              ctgGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTa                      <  1:1082745/148‑1 (MQ=255)
                                                                                                                               tgGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTAt                     >  2:613424/1‑148 (MQ=255)
                                                                                                                                ggAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTAtt                    <  2:1622049/148‑1 (MQ=255)
                                                                                                                                 gAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTATTa                   <  2:2367399/148‑1 (MQ=255)
                                                                                                                                    ttCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTATTACtt                <  1:887866/148‑1 (MQ=255)
                                                                                                                                     tctcGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTATTACttt               <  2:78714/148‑1 (MQ=255)
                                                                                                                                     tctcGAACGGTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTATTACttt               <  2:1751934/148‑1 (MQ=255)
                                                                                                                                      ctcGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTATTACTTTg              >  1:2438470/1‑148 (MQ=255)
                                                                                                                                       tcGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTATTACTTTGt             >  1:2153201/1‑148 (MQ=255)
                                                                                                                                        cGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTATTACTTTGTa            >  2:665891/1‑148 (MQ=255)
                                                                                                                                           aCGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTATTACTTTGTAAAt         >  2:1120103/1‑148 (MQ=255)
                                                                                                                                             gTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTATTACTTTGTAAATTg       >  1:1198646/1‑148 (MQ=255)
                                                                                                                                                gaagATATTTATTTTGATGTCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTATTACTTTGTAAATTGCAg    <  1:1372378/145‑1 (MQ=255)
                                                                                                                                                 aCGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTAGCAATGAGTATTACTTTGTAAATTGCAgg   <  1:2158845/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTATTACTTTGTAAATTGCAGGG  >  CP009273/4544960‑4545253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑