Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,548,084 A→T 41.5% L8* (TTG→TAG)  hypT ← hypochlorite‑responsive transcription factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,548,0840AT41.5% 115.8 / 245.1 183L8* (TTG→TAG) hypThypochlorite‑responsive transcription factor
Reads supporting (aligned to +/- strand):  ref base A (67/40);  new base T (46/30);  total (113/70)
Fisher's exact test for biased strand distribution p-value = 8.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 191 total aligned reads displayed.

TTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAGTTTG  >  CP009273/4547939‑4548228
                                                                                                                                                 |                                                                                                                                                
ttCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGTCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCaaa                                                                                                                                                <  1:2377630/148‑1 (MQ=255)
ttCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCtaa                                                                                                                                                >  2:155088/1‑148 (MQ=255)
ttCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCaaa                                                                                                                                                <  2:411624/148‑1 (MQ=255)
ttCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCAGTTGGTTTCAATATTATGCtaa                                                                                                                                                >  2:3050511/1‑148 (MQ=255)
     cacCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGc                                                                                                                                           <  2:225249/148‑1 (MQ=255)
      acCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGca                                                                                                                                          <  2:2593634/148‑1 (MQ=255)
      acCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGca                                                                                                                                          <  1:2527502/148‑1 (MQ=255)
      acCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGca                                                                                                                                          <  1:1583516/148‑1 (MQ=255)
         aaTCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGcacca                                                                                                                                       >  2:401566/1‑148 (MQ=255)
          aTCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGcaccac                                                                                                                                      >  2:2203045/1‑148 (MQ=255)
          aTCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGcaccac                                                                                                                                      <  1:1329093/148‑1 (MQ=255)
           tCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACcaca                                                                                                                                     >  2:728931/1‑148 (MQ=255)
            cGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAg                                                                                                                                    >  1:1068339/1‑148 (MQ=255)
              ccTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTc                                                                                                                                  >  1:1486917/1‑148 (MQ=255)
               cTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCa                                                                                                                                 >  1:2884317/1‑148 (MQ=255)
                tGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCAt                                                                                                                                >  2:2387028/1‑148 (MQ=255)
                    ccAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCAt                                                                                                                            <  2:477056/148‑1 (MQ=255)
                      aGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTa                                                                                                                          <  1:3220058/148‑1 (MQ=255)
                                tGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTActc                                                                                                                <  2:3287426/148‑1 (MQ=255)
                                  cggcggCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTg                                                                                                              >  1:157941/1‑148 (MQ=255)
                                        cTGAATGCCGGTTGCTAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAgcc                                                                                                        >  1:3109066/1‑148 (MQ=255)
                                           aaTGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAgccgcc                                                                                                     >  1:2584384/1‑148 (MQ=255)
                                           aaTGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAgccgcc                                                                                                     <  1:2004936/148‑1 (MQ=255)
                                             tGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCt                                                                                                   >  1:100951/1‑148 (MQ=255)
                                                    tGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGc                                                                                            <  1:2719934/148‑1 (MQ=255)
                                                     gCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCa                                                                                           <  2:995740/148‑1 (MQ=255)
                                                        agaCGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGca                                                                                                                                          >  1:1837440/1‑98 (MQ=255)
                                                          aCGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTa                                                                                      >  2:1692109/1‑148 (MQ=255)
                                                          aCGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTa                                                                                      >  2:413615/1‑148 (MQ=255)
                                                            gTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACg                                                                                    >  1:1297440/1‑148 (MQ=255)
                                                               gCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTcc                                                                                 <  1:1513413/148‑1 (MQ=255)
                                                                  actgactgCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCCTTCTACGTCCAtt                                                                              >  2:2070367/1‑148 (MQ=255)
                                                                  actgactgCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCAtt                                                                              <  2:500805/148‑1 (MQ=255)
                                                                   ctgtctgCCGCCTGTGAAAAATTGCGTCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACTTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTc                                                                             <  1:2482954/148‑1 (MQ=255)
                                                                   ctgactgCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTc                                                                             <  1:1012821/148‑1 (MQ=255)
                                                                   ctgactgCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTc                                                                             <  1:2788300/148‑1 (MQ=255)
                                                                   ctgactgCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTc                                                                             >  2:3224524/1‑148 (MQ=255)
                                                                     gactgCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCgg                                                                           >  1:1734133/1‑148 (MQ=255)
                                                                          ccgccTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGggcggc                                                                      <  2:2462521/148‑1 (MQ=255)
                                                                               tGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACa                                                                 >  1:208612/1‑148 (MQ=255)
                                                                                gggAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACaa                                                                <  1:1174617/148‑1 (MQ=255)
                                                                                gggAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACaa                                                                <  1:1175347/148‑1 (MQ=255)
                                                                                  gAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAAcc                                                              >  2:2329197/1‑148 (MQ=255)
                                                                                   aaaaaTTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCg                                                             >  1:2392126/1‑148 (MQ=255)
                                                                                    aaaaTTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGt                                                            <  1:2255721/148‑1 (MQ=255)
                                                                                     aaaTTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGtt                                                           <  1:504628/148‑1 (MQ=255)
                                                                                     aaaTTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGtt                                                           >  2:571181/1‑148 (MQ=255)
                                                                                        ttGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTaa                                                        >  1:960383/1‑148 (MQ=255)
                                                                                           cGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGcc                                                     >  2:639112/1‑148 (MQ=255)
                                                                                              cATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGtt                                                  >  1:3100944/1‑148 (MQ=255)
                                                                                                tttttCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTc                                                >  2:290838/1‑148 (MQ=255)
                                                                                                tttttCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGACAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTc                                                >  2:1646767/1‑148 (MQ=255)
                                                                                                  tttCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCaa                                              <  2:1321656/148‑1 (MQ=255)
                                                                                                     ccAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACg                                           <  2:818016/148‑1 (MQ=255)
                                                                                                       aGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGAAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGCCTGACAACCGTTTAAGCCGTTTAAAACGtt                                         >  2:2699565/1‑148 (MQ=255)
                                                                                                        gggTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTc                                        >  1:839729/1‑148 (MQ=255)
                                                                                                        gggTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTc                                        >  1:2763376/1‑148 (MQ=255)
                                                                                                         ggTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTcc                                       >  2:3147480/1‑148 (MQ=255)
                                                                                                         ggTCAGAAAATCATAAAGCCATTTGGTTACAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGGCTCCCTTGAGGGCATTCTAAGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTAAAACGTTcc                                       <  2:2037857/148‑1 (MQ=255)
                                                                                                           tCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCtt                                     >  2:555024/1‑148 (MQ=255)
                                                                                                            cAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCttt                                    >  2:1599591/1‑148 (MQ=255)
                                                                                                             aGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAACTCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTc                                   <  2:519409/148‑1 (MQ=255)
                                                                                                              gAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCa                                  >  2:1552660/1‑148 (MQ=255)
                                                                                                              gAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCa                                  >  2:1698518/1‑148 (MQ=255)
                                                                                                               aaaaTCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCAc                                 >  1:1807564/1‑148 (MQ=255)
                                                                                                                   tCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTATCACGCAg                             >  2:2442557/1‑148 (MQ=255)
                                                                                                                     aTAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGc                           <  2:604308/148‑1 (MQ=255)
                                                                                                                     aTAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGc                           >  2:2609751/1‑148 (MQ=255)
                                                                                                                      tAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCTCGCAGGCt                          >  2:266040/1‑148 (MQ=255)
                                                                                                                      tAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACCTTCCTTTCACGCAGGCt                          >  2:265567/1‑148 (MQ=255)
                                                                                                                       aaaGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCtt                         >  2:86623/1‑148 (MQ=255)
                                                                                                                       aaaGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCtt                         >  1:332808/1‑148 (MQ=255)
                                                                                                                       aaaGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCAACAATTACGTTTTACTCTGTCAGACGCCCTTGCGGTCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCATTTCACTCAGGCtt                         >  1:2270232/1‑148 (MQ=255)
                                                                                                                          gCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGc                      >  1:2679698/1‑148 (MQ=255)
                                                                                                                          gCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGAGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGc                      >  1:2423588/1‑148 (MQ=255)
                                                                                                                           ccATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCCTTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGcc                     >  2:77392/1‑148 (MQ=255)
                                                                                                                           ccATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGcc                     >  1:2913747/1‑148 (MQ=255)
                                                                                                                           ccATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGcc                     >  1:535398/1‑148 (MQ=255)
                                                                                                                           ccATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGcc                     >  2:1131795/1‑148 (MQ=255)
                                                                                                                              tttGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTc                  >  1:718854/1‑148 (MQ=255)
                                                                                                                              tttGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTc                  <  1:1910517/148‑1 (MQ=255)
                                                                                                                              tttGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTc                  >  2:3208822/1‑148 (MQ=255)
                                                                                                                              tttGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTc                  >  2:3208859/1‑148 (MQ=255)
                                                                                                                              tttGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTc                  >  1:1410164/1‑148 (MQ=255)
                                                                                                                                      cAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCa          <  2:970185/148‑1 (MQ=255)
                                                                                                                                      cAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCa          >  1:1443372/1‑148 (MQ=255)
                                                                                                                                      cAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCa          >  2:843666/1‑148 (MQ=255)
                                                                                                                                        atatTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATc        >  2:1069576/1‑148 (MQ=255)
                                                                                                                                         tattatGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCa       >  2:2776874/1‑148 (MQ=255)
                                                                                                                                          attatGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAg      >  2:3107124/1‑148 (MQ=255)
                                                                                                                                          attatGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAg      >  2:3242906/1‑148 (MQ=255)
                                                                                                                                          attatGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAg      >  2:3250346/1‑148 (MQ=255)
                                                                                                                                          attatGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAg      >  2:3059994/1‑148 (MQ=255)
                                                                                                                                           ttatGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAGt     <  2:100951/148‑1 (MQ=255)
                                                                                                                                           ttatGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAGt     <  2:2105679/148‑1 (MQ=255)
                                                                                                                                            tatGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAGtt    >  1:1844181/1‑148 (MQ=255)
                                                                                                                                            tatGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAGtt    >  1:174000/1‑148 (MQ=255)
                                                                                                                                              tGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAGTTTg  >  1:828331/1‑148 (MQ=255)
                                                                                                                                              tGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAGTTTg  >  1:1556145/1‑148 (MQ=255)
                                                                                                                                              tGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCGTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTACCTTTCACGCAGGCTTTGCCGTCTTTGAACAACAGTTTg  >  1:3194977/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAGTTTG  >  CP009273/4547939‑4548228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑