Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,555,156 G→T 100% A171E (GCG→GAG)  yjiM ← putative 2‑hydroxyglutaryl‑CoA dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,555,1560GT99.4% 586.4 / ‑4.2 178A171E (GCG→GAG) yjiMputative 2‑hydroxyglutaryl‑CoA dehydratase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (98/79);  total (99/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 178 total aligned reads displayed.

CAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACA  >  CP009273/4555011‑4555299
                                                                                                                                                 |                                                                                                                                               
cAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCa                                                                                                                                               <  2:160108/148‑1 (MQ=255)
cAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCa                                                                                                                                               <  1:1203009/148‑1 (MQ=255)
cAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCa                                                                                                                                               <  2:2850225/148‑1 (MQ=255)
 aGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCaa                                                                                                                                              <  1:3090005/148‑1 (MQ=255)
    tCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATgg                                                                                                                                           >  1:3188627/1‑148 (MQ=255)
     cATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGc                                                                                                                                          >  1:1020045/1‑148 (MQ=255)
     cATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTAAGCTCAATGGc                                                                                                                                          <  1:3232552/148‑1 (MQ=255)
     cATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTAAGCTCAATGGc                                                                                                                                          <  1:2127392/148‑1 (MQ=255)
       ttGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCAt                                                                                                                                        <  1:560077/148‑1 (MQ=255)
           tCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATcgcg                                                                                                                                    >  1:1380134/1‑148 (MQ=255)
              aCGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCag                                                                                                                                 >  1:2390240/1‑148 (MQ=255)
              aCGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCag                                                                                                                                 >  1:2388963/1‑148 (MQ=255)
                gCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCagag                                                                                                                               >  2:1443307/1‑148 (MQ=255)
                  ctctTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCa                                                                                                                             >  2:2133499/1‑148 (MQ=255)
                  ctctTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGAGAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCa                                                                                                                             >  2:2145239/1‑148 (MQ=255)
                    ctTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATc                                                                                                                           >  2:241082/1‑148 (MQ=255)
                      ttATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCtt                                                                                                                         >  1:1261549/1‑148 (MQ=255)
                       tATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTc                                                                                                                        >  1:2805544/1‑148 (MQ=255)
                       tATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTc                                                                                                                        >  1:1785069/1‑148 (MQ=255)
                        aTCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCg                                                                                                                       <  1:573544/148‑1 (MQ=255)
                          cGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCt                                                                                                                     >  1:3137377/1‑148 (MQ=255)
                           gAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTa                                                                                                                    <  1:241181/148‑1 (MQ=255)
                            aaCCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTaa                                                                                                                   <  2:378626/148‑1 (MQ=255)
                             aCCGGAAGGTTGCGCCGTAAACCACTTTCAGAAGGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAAt                                                                                                                  <  2:1776345/148‑1 (MQ=255)
                                  aaGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGt                                                                                                             >  2:2208112/1‑148 (MQ=255)
                                    ggTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGc                                                                                                           <  2:1272579/148‑1 (MQ=255)
                                    ggTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGc                                                                                                           >  1:1874298/1‑148 (MQ=255)
                                    ggTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGc                                                                                                           >  1:1874510/1‑148 (MQ=255)
                                      ttGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTGCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGccc                                                                                                         >  1:613462/1‑148 (MQ=255)
                                       tGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCa                                                                                                        >  2:461136/1‑148 (MQ=255)
                                         cgcCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCaaa                                                                                                      <  1:683745/148‑1 (MQ=255)
                                            cGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACg                                                                                                   <  1:233495/148‑1 (MQ=255)
                                              tAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGtt                                                                                                 >  2:3037636/1‑148 (MQ=255)
                                              tAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGtt                                                                                                 >  1:2029387/1‑148 (MQ=255)
                                               aaaCCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGttc                                                                                                >  1:2179992/1‑148 (MQ=255)
                                               aaaCCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGttc                                                                                                >  2:1107013/1‑148 (MQ=255)
                                                aaCCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGttct                                                                                               <  1:2319265/148‑1 (MQ=255)
                                                 aCCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGttctt                                                                                              >  1:2426439/1‑148 (MQ=255)
                                                  ccACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGttcttc                                                                                             <  1:2990252/148‑1 (MQ=255)
                                                  ccACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGttcttc                                                                                             >  2:1404425/1‑148 (MQ=255)
                                                   cACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTtcttct                                                                                            <  2:693045/148‑1 (MQ=255)
                                                   cACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTtcttct                                                                                            <  2:2898198/148‑1 (MQ=255)
                                                    aCTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTa                                                                                           <  1:2995018/148‑1 (MQ=255)
                                                       ttCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCg                                                                                        <  1:2947713/148‑1 (MQ=255)
                                                        tCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGt                                                                                       <  2:3189124/148‑1 (MQ=255)
                                                        tCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGt                                                                                       <  2:3188627/148‑1 (MQ=255)
                                                           gAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGtttt                                                                                    <  1:2522308/148‑1 (MQ=255)
                                                            aaTGTCGCTGCCGCTAATCGCCTGAGGATTTAACTGCCCAATATGATCAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGAAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGttttt                                                                                   >  2:2963249/1‑148 (MQ=255)
                                                            aaTGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGttttt                                                                                   <  1:697322/148‑1 (MQ=255)
                                                              tGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTg                                                                                 >  1:2787754/1‑148 (MQ=255)
                                                                  gCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAg                                                                             <  2:1792323/148‑1 (MQ=255)
                                                                     gCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAgcgc                                                                          <  1:1338532/148‑1 (MQ=255)
                                                                           aaGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCAtc                                                                    >  1:926635/1‑148 (MQ=255)
                                                                           aaGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCAAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCAtc                                                                    >  1:3182611/1‑148 (MQ=255)
                                                                             gcgcCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCAtctc                                                                  >  1:453600/1‑148 (MQ=255)
                                                                                ccGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGc                                                               <  1:39584/148‑1 (MQ=255)
                                                                                  ggaggaTTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCtt                                                             >  2:2690459/1‑148 (MQ=255)
                                                                                   gaggaTTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCttt                                                            >  2:2766132/1‑148 (MQ=255)
                                                                                    aggaTTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTc                                                           >  1:2734797/1‑148 (MQ=255)
                                                                                      gaTTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGACATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCa                                                         >  1:880629/1‑148 (MQ=255)
                                                                                       aTTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCAt                                                        >  1:1755553/1‑148 (MQ=255)
                                                                                       aTTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCAt                                                        >  1:1755570/1‑148 (MQ=255)
                                                                                         ttAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATaa                                                      >  2:3103491/1‑148 (MQ=255)
                                                                                          tAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAac                                                     >  1:3017314/1‑148 (MQ=255)
                                                                                                cccAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCAACCGTTTTTTGCAAGCGCAGCAACTCGGCTTTCAATAactcacgc                                               >  2:3211555/1‑148 (MQ=255)
                                                                                                  cAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCNCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGa                                             >  1:1680697/1‑148 (MQ=255)
                                                                                                  cAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGa                                             >  1:1667229/1‑148 (MQ=255)
                                                                                                   aaGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAg                                            >  1:1879752/1‑148 (MQ=255)
                                                                                                       tgatAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCAt                                        >  2:3083360/1‑148 (MQ=255)
                                                                                                       agatAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCAt                                        <  1:1011851/147‑1 (MQ=255)
                                                                                                        gatAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATc                                       <  2:1046499/148‑1 (MQ=255)
                                                                                                               aTTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTa                                >  2:1885682/1‑148 (MQ=255)
                                                                                                                ttAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTaa                               >  1:2836718/1‑148 (MQ=255)
                                                                                                                ttAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTaa                               <  2:1785069/148‑1 (MQ=255)
                                                                                                                ttAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTaa                               >  2:512688/1‑148 (MQ=255)
                                                                                                                ttAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTaa                               >  1:3241011/1‑148 (MQ=255)
                                                                                                                ttAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTaa                               <  1:1582986/148‑1 (MQ=255)
                                                                                                                ttAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTaa                               <  2:2812334/148‑1 (MQ=255)
                                                                                                                ttAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTaa                               <  1:772080/148‑1 (MQ=255)
                                                                                                                 tAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAAc                              >  1:67269/1‑148 (MQ=255)
                                                                                                                 tAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAAc                              >  2:2974208/1‑148 (MQ=255)
                                                                                                                 tAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAAc                              >  2:2158381/1‑148 (MQ=255)
                                                                                                                 tAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAAc                              >  2:1325923/1‑148 (MQ=255)
                                                                                                                   gCCAGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGc                            >  2:1407918/1‑148 (MQ=255)
                                                                                                                      aGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGt                         <  2:1862799/148‑1 (MQ=255)
                                                                                                                      aGTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGt                         <  2:1858771/148‑1 (MQ=255)
                                                                                                                       gTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGtt                        <  1:2133499/148‑1 (MQ=255)
                                                                                                                       gTGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGGGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGtt                        <  1:2145239/148‑1 (MQ=255)
                                                                                                                        tGCGCGACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTg                       >  2:3161312/1‑148 (MQ=255)
                                                                                                                            cgACGTTCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCa                   <  2:2076717/148‑1 (MQ=255)
                                                                                                                                 ttCGCGGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGc              >  1:3236221/1‑148 (MQ=255)
                                                                                                                                    gcgGTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATc           <  1:2208112/148‑1 (MQ=255)
                                                                                                                                       gTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATcaca        <  2:1189662/148‑1 (MQ=255)
                                                                                                                                       gTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATcaca        <  2:889280/148‑1 (MQ=255)
                                                                                                                                       gTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATcaca        >  1:1875794/1‑148 (MQ=255)
                                                                                                                                       gTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATcaca        <  1:2754799/148‑1 (MQ=255)
                                                                                                                                       gTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATcaca        <  2:2603883/148‑1 (MQ=255)
                                                                                                                                       gTTTTTCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATcaca        <  2:2603856/148‑1 (MQ=255)
                                                                                                                                          tttCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGa     >  2:687403/1‑148 (MQ=255)
                                                                                                                                            tCAGCTCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAAc   >  2:537555/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTAAGCGCCGGAGGATTTAACTGCCCAAGATGATAAAAATTAGCCAGTGCGCGACGTTCGCGGTTTTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACA  >  CP009273/4555011‑4555299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑