Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,563,898 G→T 9.2% pseudogene (163/2937 nt) yjiV → pseudogene; conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,563,8980GT 9.2% 784.4 / 35.0 224pseudogene (163/2937 nt)yjiVpseudogene; conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (102/101);  new base T (12/9);  total (114/110)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 230 total aligned reads displayed.

AAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCAC  >  CP009273/4563751‑4564045
                                                                                                                                                   |                                                                                                                                                   
aaaCAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAg                                                                                                                                                     >  2:95174/1‑148 (MQ=255)
aaaCAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAg                                                                                                                                                     <  1:3270587/148‑1 (MQ=255)
 aaCAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGa                                                                                                                                                    <  1:363412/148‑1 (MQ=255)
  aCAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAatat                                                                                                                                                   >  1:833499/1‑148 (MQ=255)
    aGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTg                                                                                                                                                 >  2:1893039/1‑148 (MQ=255)
         tACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGAt                                                                                                                                            >  2:2858167/1‑148 (MQ=255)
           cagccagcAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTa                                                                                                                                          >  1:1563253/1‑148 (MQ=255)
           cagccagcAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGAGACAGAGTGCAGATTa                                                                                                                                          >  1:2263258/1‑148 (MQ=255)
            agccagcAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTaa                                                                                                                                         <  1:2881840/148‑1 (MQ=255)
              ccagcAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAg                                                                                                                                       <  1:966994/148‑1 (MQ=255)
               cagcagGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGc                                                                                                                                      <  1:2017495/148‑1 (MQ=255)
                agcagGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCa                                                                                                                                     <  2:555191/148‑1 (MQ=255)
                 gcagGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCAc                                                                                                                                    >  1:1451798/1‑148 (MQ=255)
                   agGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACgg                                                                                                                                  >  1:687885/1‑148 (MQ=255)
                    ggAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGt                                                                                                                                 <  1:1606113/148‑1 (MQ=255)
                     gAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAATATTGCAGATTAAAGCACGGTa                                                                                                                                >  2:2314132/1‑148 (MQ=255)
                     gAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTa                                                                                                                                >  2:280276/1‑148 (MQ=255)
                     gAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTa                                                                                                                                >  2:1224667/1‑148 (MQ=255)
                           cTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAATATTGCAGATTAAAGCACGGTACACCGc                                                                                                                          <  1:2365786/148‑1 (MQ=255)
                              cGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAATATTGCAGATTAAAGCACGGTACACCGCTCg                                                                                                                       <  1:2763368/148‑1 (MQ=255)
                                 tGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACa                                                                                                                    >  2:2624511/1‑148 (MQ=255)
                                  ggATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACaa                                                                                                                   <  2:2529571/148‑1 (MQ=255)
                                   gATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACaaa                                                                                                                  >  2:1283313/1‑148 (MQ=255)
                                   gATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACaaa                                                                                                                  >  2:1922559/1‑148 (MQ=255)
                                    aTCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAg                                                                                                                 >  1:1744071/1‑148 (MQ=255)
                                      cGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGc                                                                                                               >  2:581729/1‑148 (MQ=255)
                                        aTGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTg                                                                                                             >  1:1169437/1‑148 (MQ=255)
                                        aTGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTg                                                                                                             <  1:2646251/148‑1 (MQ=255)
                                          gTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGc                                                                                                           >  1:653290/1‑148 (MQ=255)
                                           tCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGcc                                                                                                          >  1:1432057/1‑148 (MQ=255)
                                                   gggACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCt                                                                                                  <  1:2197520/148‑1 (MQ=255)
                                                   gggACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCt                                                                                                  <  1:586062/148‑1 (MQ=255)
                                                     gACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCtat                                                                                                >  1:2099373/1‑148 (MQ=255)
                                                      aCTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCtatg                                                                                               <  2:1688901/148‑1 (MQ=255)
                                                        tCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCtatgtg                                                                                             <  2:856034/148‑1 (MQ=255)
                                                           aCCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCtatgtgtat                                                                                          <  2:1082616/148‑1 (MQ=255)
                                                           aCCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCtatgtgtat                                                                                          >  1:1093371/1‑148 (MQ=255)
                                                             cTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCtatgtgtatgt                                                                                        <  1:1584523/148‑1 (MQ=255)
                                                                 tGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGa                                                                                      >  1:1873309/1‑146 (MQ=255)
                                                                 tGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAAc                                                                                    <  1:1700712/148‑1 (MQ=255)
                                                                    aaaGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAg                                                                                 <  1:661697/148‑1 (MQ=255)
                                                                      aGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCg                                                                               >  2:2167035/1‑148 (MQ=255)
                                                                        gATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAATATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGAc                                                                             >  1:1663483/1‑148 (MQ=255)
                                                                        gATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAATATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGAc                                                                             >  1:2662489/1‑148 (MQ=255)
                                                                        gATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGAc                                                                             >  2:101716/1‑148 (MQ=255)
                                                                               cATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATtg                                                                      >  2:1393504/1‑148 (MQ=255)
                                                                               cATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATtg                                                                      <  2:2479379/148‑1 (MQ=255)
                                                                                  ttCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATTTGAACCAGCGACAAAATTGTGa                                                                   >  1:1840597/1‑148 (MQ=255)
                                                                                  ttCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGa                                                                   <  2:3144062/148‑1 (MQ=255)
                                                                                     ttAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACtg                                                                <  1:1290223/148‑1 (MQ=255)
                                                                                      tAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACtgt                                                               <  1:81116/148‑1 (MQ=255)
                                                                                      tAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACtgt                                                               >  1:1534963/1‑148 (MQ=255)
                                                                                      tAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACtgt                                                               <  2:1073803/148‑1 (MQ=255)
                                                                                          gTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGaa                                                           <  2:567618/148‑1 (MQ=255)
                                                                                            cAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGc                                                         >  1:559636/1‑148 (MQ=255)
                                                                                            cAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGc                                                         <  1:247856/148‑1 (MQ=255)
                                                                                             aGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGcc                                                        <  2:3227757/148‑1 (MQ=255)
                                                                                               ggCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCt                                                      >  1:3203378/1‑148 (MQ=255)
                                                                                               ggCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCt                                                      >  1:258109/1‑148 (MQ=255)
                                                                                               ggCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCt                                                      >  1:1008783/1‑148 (MQ=255)
                                                                                                   cATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGtat                                                  <  2:1432057/148‑1 (MQ=255)
                                                                                                    atatCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGtata                                                 <  1:537218/148‑1 (MQ=255)
                                                                                                       tCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGa                                              >  1:176079/1‑148 (MQ=255)
                                                                                                         ttCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAg                                            <  2:3007422/148‑1 (MQ=255)
                                                                                                          tCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAATATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGt                                           >  1:259943/1‑148 (MQ=255)
                                                                                                           ccAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACGAAATTGTGACTGTGGAAGCCCTGTATACGAAGtt                                          >  2:2515941/1‑148 (MQ=255)
                                                                                                           ccAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGtt                                          >  2:2515998/1‑148 (MQ=255)
                                                                                                           ccAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGtt                                          >  1:3108350/1‑148 (MQ=255)
                                                                                                              gCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCa                                       <  2:46843/148‑1 (MQ=255)
                                                                                                               cGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGATGCAt                                      >  1:1641225/1‑148 (MQ=255)
                                                                                                                gTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCAtt                                     <  2:1098438/148‑1 (MQ=255)
                                                                                                                gTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGGTGCAtt                                     <  2:1744199/148‑1 (MQ=255)
                                                                                                                   aTACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTg                                  <  2:292625/148‑1 (MQ=255)
                                                                                                                   aTACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTg                                  >  2:235497/1‑148 (MQ=255)
                                                                                                                    tACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGt                                 >  1:1350894/1‑148 (MQ=255)
                                                                                                                      ccTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTaa                               >  2:2219962/1‑148 (MQ=255)
                                                                                                                      ccTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTaa                               <  1:3136862/148‑1 (MQ=255)
                                                                                                                       cTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAAt                              <  2:920263/148‑1 (MQ=255)
                                                                                                                        tAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATg                             <  1:1021598/148‑1 (MQ=255)
                                                                                                                        tAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATg                             <  2:2500068/148‑1 (MQ=255)
                                                                                                                        tAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATg                             <  1:124364/148‑1 (MQ=255)
                                                                                                                         aGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGa                            <  2:213106/148‑1 (MQ=255)
                                                                                                                         aGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGa                            >  2:3114825/1‑148 (MQ=255)
                                                                                                                         aGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGa                            <  2:1728228/148‑1 (MQ=255)
                                                                                                                           ggAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGAtt                          >  1:2843144/1‑148 (MQ=255)
                                                                                                                            gAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTg                         <  1:479968/148‑1 (MQ=255)
                                                                                                                               tatGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTaa                      <  2:867196/148‑1 (MQ=255)
                                                                                                                                  gggCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGa                   <  2:2609226/148‑1 (MQ=255)
                                                                                                                                  gggCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGa                   >  1:309561/1‑148 (MQ=255)
                                                                                                                                  gggCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGa                   <  2:1503809/148‑1 (MQ=255)
                                                                                                                                    gCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGc                 >  1:1965016/1‑148 (MQ=255)
                                                                                                                                      ttGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCt               <  2:258109/148‑1 (MQ=255)
                                                                                                                                       tgtgTTGATAAATATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTc              <  2:212059/148‑1 (MQ=255)
                                                                                                                                       tgtgTTGATAAATATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTc              <  2:626786/148‑1 (MQ=255)
                                                                                                                                       tgtgTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTc              >  1:2316147/1‑148 (MQ=255)
                                                                                                                                           ttGATAAATATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCAtct          >  2:77930/1‑148 (MQ=255)
                                                                                                                                                aaaGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTgg     >  2:1142964/1‑148 (MQ=255)
                                                                                                                                                aaaGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTgg     >  2:2047758/1‑148 (MQ=255)
                                                                                                                                                aaaGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTgg     >  2:2121124/1‑148 (MQ=255)
                                                                                                                                                   gATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCAc  >  2:2241778/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCAC  >  CP009273/4563751‑4564045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑