Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,589,130 G→T 84.2% I43I (ATC→ATA)  opgB ← phosphoglycerol transferases I and II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,589,1300GT84.2% 431.5 / 86.0 205I43I (ATC→ATA) opgBphosphoglycerol transferases I and II
Reads supporting (aligned to +/- strand):  ref base G (18/15);  new base T (103/69);  total (121/84)
Fisher's exact test for biased strand distribution p-value = 5.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 206 total aligned reads displayed.

CCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTA  >  CP009273/4588984‑4589274
                                                                                                                                                  |                                                                                                                                                
ccGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGa                                                                                                                                                 >  2:3064721/1‑148 (MQ=255)
 cGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTat                                                                                                                                                <  1:1983065/148‑1 (MQ=255)
  gAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTtata                                                                                                                                               >  2:2411409/1‑148 (MQ=255)
   aaCACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTatat                                                                                                                                              >  1:3130923/1‑148 (MQ=255)
      acCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTa                                                                                                                                           <  2:2688751/148‑1 (MQ=255)
        cGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTaaa                                                                                                                                         >  1:2120521/1‑148 (MQ=255)
        cGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTaaa                                                                                                                                         <  1:1323776/148‑1 (MQ=255)
                  cccAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATa                                                                                                                               <  2:2545044/148‑1 (MQ=255)
                  cccAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATa                                                                                                                               <  2:3105102/148‑1 (MQ=255)
                   ccAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAg                                                                                                                              >  2:2722919/1‑148 (MQ=255)
                   ccAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAg                                                                                                                              <  1:267735/148‑1 (MQ=255)
                      gCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGccc                                                                                                                           <  2:2465316/148‑1 (MQ=255)
                      gCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGccc                                                                                                                           >  1:6059/1‑148 (MQ=255)
                       cacaATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGcccc                                                                                                                          <  2:1718499/148‑1 (MQ=255)
                       cacaATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGcccc                                                                                                                          <  2:1988230/148‑1 (MQ=255)
                           aTGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCa                                                                                                                      >  2:1655550/1‑148 (MQ=255)
                             gCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCAcc                                                                                                                    >  1:2037885/1‑148 (MQ=255)
                               cGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGt                                                                                                                  >  1:763122/1‑148 (MQ=255)
                                   aCCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAAc                                                                                                              >  1:2418932/1‑148 (MQ=255)
                                    cccGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACg                                                                                                             >  2:2493259/1‑148 (MQ=255)
                                    cccGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACg                                                                                                             >  2:836406/1‑148 (MQ=255)
                                       ggCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTgg                                                                                                          >  2:944210/1‑148 (MQ=255)
                                        gaaaaaTGTATTTGCTGACGCCAGCACCGGTCAGGGTGTTGGTTAAGGTATAGAGAACCGCGTCTTTAATCCCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGc                                                                                                         <  1:2584894/146‑1 (MQ=255)
                                            aaTGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCa                                                                                                     >  2:2204037/1‑148 (MQ=255)
                                            aaTGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCa                                                                                                     >  2:2227488/1‑148 (MQ=255)
                                            aaTGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCa                                                                                                     >  2:1793445/1‑148 (MQ=255)
                                             aTGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCaa                                                                                                    >  2:3197398/1‑148 (MQ=255)
                                              tGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCaaa                                                                                                   >  1:2876974/1‑148 (MQ=255)
                                               gTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAac                                                                                                  >  2:3287714/1‑148 (MQ=255)
                                                tATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACCAATAGCCCCAGCACCGTTAACGTGGCTGCAAacc                                                                                                 >  2:2673257/1‑148 (MQ=255)
                                                   ttGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAaccacc                                                                                              >  1:669354/1‑148 (MQ=255)
                                                        gACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGgtg                                                                                         >  1:1879110/1‑148 (MQ=255)
                                                        gACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGgtg                                                                                         >  2:2211788/1‑148 (MQ=255)
                                                         aCGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATATAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGgtgt                                                                                        <  2:1797338/148‑1 (MQ=255)
                                                         aCGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGgtgt                                                                                        >  2:1378246/1‑148 (MQ=255)
                                                                cACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTcc                                                                                 >  2:2286471/1‑148 (MQ=255)
                                                                cACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTcc                                                                                 >  1:2307703/1‑148 (MQ=255)
                                                                cACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTcc                                                                                 >  1:2305489/1‑148 (MQ=255)
                                                                 aCCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCGGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCCTTAACGTGGCTGCAAACCACCAGGTGTTACGTccc                                                                                >  1:103429/1‑148 (MQ=255)
                                                                      tCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCttt                                                                           >  2:8498/1‑148 (MQ=255)
                                                                         ggCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCa                                                                        <  1:2470988/148‑1 (MQ=255)
                                                                         ggCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCa                                                                        >  1:879417/1‑148 (MQ=255)
                                                                         ggCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCa                                                                        <  2:2846626/148‑1 (MQ=255)
                                                                         ggCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGAGGCTGCAAACCACCAGGAGTTACGTCCAGCTTTACa                                                                        >  2:964059/1‑148 (MQ=255)
                                                                          gCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCAt                                                                       >  2:2696656/1‑148 (MQ=255)
                                                                          gCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAAAAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCAt                                                                       >  2:1415702/1‑148 (MQ=255)
                                                                          gCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCAt                                                                       <  2:1186191/148‑1 (MQ=255)
                                                                           cTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATg                                                                      >  1:2996194/1‑148 (MQ=255)
                                                                              ttGGTTAAGTTATATACAACCGCGTCTTTAATCCCATCGCCATTAAAATAGTCGCTGGAAAACAGGTTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGt                                                                   <  1:2224715/148‑1 (MQ=255)
                                                                              ttGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGt                                                                   >  1:1460385/1‑148 (MQ=255)
                                                                              ttGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGt                                                                   >  1:118315/1‑148 (MQ=255)
                                                                               tGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTa                                                                  >  1:519840/1‑148 (MQ=255)
                                                                                ggTTAAGGTATAGAGAACCGCGTCTTTAATACCATCGCCAGTAAAATAGTCGCTGCAAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTaa                                                                 <  1:1154575/148‑1 (MQ=255)
                                                                                ggTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTaa                                                                 <  1:1214588/148‑1 (MQ=255)
                                                                                 gTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTaaa                                                                <  2:2108119/148‑1 (MQ=255)
                                                                                  ttAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAAt                                                               <  2:789300/148‑1 (MQ=255)
                                                                                  ttAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAAt                                                               <  2:2062214/148‑1 (MQ=255)
                                                                                    aaGTTATATAGAACCGTGTCGTTAATACATTCCCGAGTAATATTGTCGCTGTAAAACAGGTTTATATTTAAAACGAAAATTAGCCCCAGCACCGTTAACGTGTCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCa                                                             <  1:307344/148‑1 (MQ=255)
                                                                                       ttataGAGAACCGCGTCTTTAATACCATCGCCATTAAAATCGTCGCGGGCAAACCGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGca                                                          <  1:847304/147‑1 (MQ=255)
                                                                                       gTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGca                                                          >  2:1492883/1‑148 (MQ=255)
                                                                                        tataGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGcac                                                         >  2:2857080/1‑148 (MQ=255)
                                                                                          taGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACag                                                       <  1:2979914/148‑1 (MQ=255)
                                                                                          taGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCGGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACag                                                       <  2:842220/148‑1 (MQ=255)
                                                                                            gagaACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACagag                                                     <  1:2735680/148‑1 (MQ=255)
                                                                                            gagaACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACagag                                                     >  2:3159287/1‑148 (MQ=255)
                                                                                             agaACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAgg                                                    >  1:1827296/1‑148 (MQ=255)
                                                                                               aaCCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCg                                                  <  1:1699808/148‑1 (MQ=255)
                                                                                               aaCCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCg                                                  >  2:2955825/1‑148 (MQ=255)
                                                                                                aCCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCga                                                 >  2:3084859/1‑148 (MQ=255)
                                                                                                  cgcgTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCgaga                                               <  2:170176/148‑1 (MQ=255)
                                                                                                    cgtcgtTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGaaa                                             >  2:979008/1‑148 (MQ=255)
                                                                                                     gtcgtTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGaaaa                                            <  2:1197256/148‑1 (MQ=255)
                                                                                                       cgtTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAg                                          <  2:419668/148‑1 (MQ=255)
                                                                                                       cgtTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAg                                          <  2:1523799/148‑1 (MQ=255)
                                                                                                        tttAATCCCATCGCCATTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAgg                                         <  1:585852/147‑1 (MQ=255)
                                                                                                         ttAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAggg                                        >  1:3116426/1‑148 (MQ=255)
                                                                                                         ttAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAggg                                        <  2:921229/148‑1 (MQ=255)
                                                                                                          tAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGc                                       >  1:2802192/1‑148 (MQ=255)
                                                                                                           aaTACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCg                                      <  1:927962/148‑1 (MQ=255)
                                                                                                            aTACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGa                                     <  2:1208018/148‑1 (MQ=255)
                                                                                                              aCCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGaaa                                   >  1:1192445/1‑148 (MQ=255)
                                                                                                                cATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAaga                                 <  2:3130923/148‑1 (MQ=255)
                                                                                                                    gCCAGTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGagt                             <  2:6059/148‑1 (MQ=255)
                                                                                                                        gTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGtt                         <  1:1422851/148‑1 (MQ=255)
                                                                                                                        gTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGtt                         >  1:512571/1‑148 (MQ=255)
                                                                                                                        gTAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGtt                         <  2:1305334/148‑1 (MQ=255)
                                                                                                                         tAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTCGTTc                        >  2:2591060/1‑148 (MQ=255)
                                                                                                                         tAAAATAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTc                        <  2:1879110/148‑1 (MQ=255)
                                                                                                                          aaaaTAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCt                       >  1:3229155/1‑148 (MQ=255)
                                                                                                                              tAGTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACa                   >  2:53747/1‑148 (MQ=255)
                                                                                                                               aGTCGCTGGCAAACAGGGTTAAAATTAAAACCAAAAATAGCCCCAGCACCGTTAACGTGGCTTCAAACCACCAGCTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTATTTCTGACaa                  >  1:3267046/1‑148 (MQ=255)
                                                                                                                                gTCGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAAc                 >  1:2141721/1‑148 (MQ=255)
                                                                                                                                  cGCTGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACgg               >  1:2515665/1‑148 (MQ=255)
                                                                                                                                     tGGCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCg            >  2:614475/1‑148 (MQ=255)
                                                                                                                                       gCAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGAt          >  1:2132704/1‑148 (MQ=255)
                                                                                                                                        cAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATc         >  2:1426693/1‑148 (MQ=255)
                                                                                                                                        cAAACAGGGTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATc         <  2:2978365/148‑1 (MQ=255)
                                                                                                                                           aCAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTc      >  2:1018220/1‑148 (MQ=255)
                                                                                                                                            cAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGGGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCa     <  2:732277/148‑1 (MQ=255)
                                                                                                                                               ggTTATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTa  >  1:5407/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTA  >  CP009273/4588984‑4589274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑