Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,590,074 A→C 12.8% V240G (GTA→GGA)  dnaC ← DNA biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,590,0740AC12.8% 479.5 / 74.8 204V240G (GTA→GGA) dnaCDNA biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base A (103/75);  new base C (14/12);  total (117/87)
Fisher's exact test for biased strand distribution p-value = 8.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

Only 100 of 206 total aligned reads displayed.

GACGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAG  >  CP009273/4589927‑4590220
                                                                                                                                                   |                                                                                                                                                  
gACGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTa                                                                                                                                                    <  2:2652028/148‑1 (MQ=255)
      gcaTGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGc                                                                                                                                              <  1:3217466/148‑1 (MQ=255)
      gcaTGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGc                                                                                                                                              >  2:199968/1‑148 (MQ=255)
       caTGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCt                                                                                                                                             <  2:937570/148‑1 (MQ=255)
          gCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACg                                                                                                                                          >  1:318897/1‑148 (MQ=255)
              cccTGTGGAAATTAATGCGCGGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAAGTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAg                                                                                                                                      <  2:1951595/148‑1 (MQ=255)
                cGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCt                                                                                                                                    >  2:2408611/1‑148 (MQ=255)
                         ttAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGtt                                                                                                                           >  2:2066609/1‑148 (MQ=255)
                          tAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGACACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTg                                                                                                                          >  2:2139605/1‑148 (MQ=255)
                                gcTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATc                                                                                                                    <  2:1375711/148‑1 (MQ=255)
                                gcTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATc                                                                                                                    <  2:222969/148‑1 (MQ=255)
                                  tGGCGGCACTGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCAc                                                                                                                  <  2:2335582/148‑1 (MQ=255)
                                   ggcggcAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCAcc                                                                                                                 <  2:2453940/148‑1 (MQ=255)
                                    gcggcAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCAccc                                                                                                                >  2:828906/1‑148 (MQ=255)
                                    gcggcAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCAccc                                                                                                                >  2:765925/1‑148 (MQ=255)
                                        cAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACaa                                                                                                            >  1:1477167/1‑148 (MQ=255)
                                        cAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACaa                                                                                                            <  1:574772/148‑1 (MQ=255)
                                        cAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACaa                                                                                                            <  2:2557205/148‑1 (MQ=255)
                                          aTGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAAc                                                                                                          >  1:84307/1‑148 (MQ=255)
                                           tGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACt                                                                                                         >  2:1465514/1‑148 (MQ=255)
                                            ggCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTg                                                                                                        >  1:163299/1‑148 (MQ=255)
                                             gcacagcacaGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGt                                                                                                       >  1:226201/1‑148 (MQ=255)
                                             gcacagcacaGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGt                                                                                                       >  1:1353258/1‑148 (MQ=255)
                                              cacagcacagAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGtt                                                                                                      >  2:2311682/1‑148 (MQ=255)
                                              cacagcacagAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGtt                                                                                                      <  1:2358682/148‑1 (MQ=255)
                                               acagcacagAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGAAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTa                                                                                                     <  1:1396649/148‑1 (MQ=255)
                                                cagcacagAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTAc                                                                                                    >  1:1754084/1‑148 (MQ=255)
                                                 agcacagAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTAcc                                                                                                   <  1:768396/148‑1 (MQ=255)
                                                 agcacagAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTAcc                                                                                                   <  2:116879/148‑1 (MQ=255)
                                                   cacagAAGATGTTTAACAGTTTTCATCATCATTACACAAGGTGGAATTGTGTCGCAGAATACCGCCAGAAACGGGGATTAATACACTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGATGAAGATCACCCACAAACTGATAcaca                                                                                                 >  1:2060873/1‑148 (MQ=255)
                                                     cagAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAgg                                                                                               >  1:296874/1‑148 (MQ=255)
                                                          gaTGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCAt                                                                                          >  1:2415449/1‑148 (MQ=255)
                                                          gaTGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCAt                                                                                          >  1:3280041/1‑148 (MQ=255)
                                                           aTGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATa                                                                                         >  2:1623110/1‑148 (MQ=255)
                                                            tGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATAc                                                                                        <  1:483325/148‑1 (MQ=255)
                                                             gTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACg                                                                                       <  1:1975406/148‑1 (MQ=255)
                                                             gTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACg                                                                                       >  2:2290427/1‑148 (MQ=255)
                                                             gTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACg                                                                                       >  2:1900531/1‑148 (MQ=255)
                                                             gTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCAAGGCGCATACg                                                                                       >  2:2658441/1‑148 (MQ=255)
                                                               ttAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGt                                                                                     >  1:379163/1‑148 (MQ=255)
                                                                tAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTc                                                                                    <  2:624599/148‑1 (MQ=255)
                                                                 aaCAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTcc                                                                                   >  1:907759/1‑148 (MQ=255)
                                                                    aGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATc                                                                                <  1:3061570/148‑1 (MQ=255)
                                                                     gTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCa                                                                               <  1:946383/148‑1 (MQ=255)
                                                                      ttttCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATc                                                                                >  1:1967393/1‑146 (MQ=255)
                                                                      ttttCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCACCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATc                                                                                <  2:1967417/146‑1 (MQ=255)
                                                                        ttCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCAcgc                                                                            >  2:1985393/1‑148 (MQ=255)
                                                                        ttCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCAcgc                                                                            >  2:318080/1‑148 (MQ=255)
                                                                          catcatcatTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGt                                                                          >  2:2402125/1‑148 (MQ=255)
                                                                           atcatcatTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGtt                                                                         >  1:2913708/1‑148 (MQ=255)
                                                                            tcatcatTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTc                                                                        >  1:129285/1‑148 (MQ=255)
                                                                             catcatTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCg                                                                       >  2:1309246/1‑148 (MQ=255)
                                                                             catcatTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCg                                                                       >  1:2621441/1‑148 (MQ=255)
                                                                             catcatTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCg                                                                       >  2:440908/1‑148 (MQ=255)
                                                                                catTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGccc                                                                    >  1:2570161/1‑148 (MQ=255)
                                                                                  ttACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAg                                                                  >  2:3211600/1‑148 (MQ=255)
                                                                                   tACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGc                                                                 >  2:1407983/1‑148 (MQ=255)
                                                                                   tACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGc                                                                 >  2:873843/1‑148 (MQ=255)
                                                                                        aaGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACtt                                                            <  1:226904/148‑1 (MQ=255)
                                                                                          ggTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTgg                                                          >  1:2118045/1‑148 (MQ=255)
                                                                                          ggTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTgg                                                          <  2:651014/148‑1 (MQ=255)
                                                                                           gTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGt                                                         >  1:3241698/1‑148 (MQ=255)
                                                                                                 ttGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCAtctc                                                   <  2:63342/148‑1 (MQ=255)
                                                                                                 ttGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCAtctc                                                   <  2:1737508/148‑1 (MQ=255)
                                                                                                  tgtgTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATctct                                                  >  2:270441/1‑148 (MQ=255)
                                                                                                  tgtgTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATctct                                                  >  2:2400363/1‑148 (MQ=255)
                                                                                                   gtgtCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCtt                                                 <  1:2131705/148‑1 (MQ=255)
                                                                                                    tgtCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTc                                                >  2:2902121/1‑148 (MQ=255)
                                                                                                       cGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCat                                             <  2:2771739/148‑1 (MQ=255)
                                                                                                       cGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCat                                             >  1:1163184/1‑148 (MQ=255)
                                                                                                        gCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCata                                            <  1:1561623/148‑1 (MQ=255)
                                                                                                         cAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCatat                                           >  2:2825503/1‑148 (MQ=255)
                                                                                                           gTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTa                                         <  1:832079/148‑1 (MQ=255)
                                                                                                            tataCCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTAc                                        >  2:3110454/1‑148 (MQ=255)
                                                                                                              taCCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTg                                      >  1:309932/1‑148 (MQ=255)
                                                                                                               aCCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGt                                     >  2:1054856/1‑148 (MQ=255)
                                                                                                               aCCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGt                                     >  1:1118211/1‑148 (MQ=255)
                                                                                                                ccGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGtt                                    <  2:2281765/148‑1 (MQ=255)
                                                                                                                   ccAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGt                                 <  2:1684805/148‑1 (MQ=255)
                                                                                                                     aGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCa                               >  1:652056/1‑148 (MQ=255)
                                                                                                                      gAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAg                              >  1:3010605/1‑148 (MQ=255)
                                                                                                                       aaaCGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGc                             <  1:1991903/148‑1 (MQ=255)
                                                                                                                       aaaCGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGc                             <  2:914783/148‑1 (MQ=255)
                                                                                                                       aaaCGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGc                             >  1:2702152/1‑148 (MQ=255)
                                                                                                                        aaCGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCa                            <  1:2955520/148‑1 (MQ=255)
                                                                                                                         aCGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCAt                           >  1:639634/1‑148 (MQ=255)
                                                                                                                           ggggATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATcc                         <  1:199968/148‑1 (MQ=255)
                                                                                                                                ttAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGtt                    >  1:3029781/1‑148 (MQ=255)
                                                                                                                                    tACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGc                >  2:2272001/1‑148 (MQ=255)
                                                                                                                                    tACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGc                >  1:2503682/1‑148 (MQ=255)
                                                                                                                                    tACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGc                >  2:2521724/1‑148 (MQ=255)
                                                                                                                                      ctctTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGt              <  1:1997353/148‑1 (MQ=255)
                                                                                                                                      ctctTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGt              <  2:3010605/148‑1 (MQ=255)
                                                                                                                                      ctctTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGt              <  1:1246917/148‑1 (MQ=255)
                                                                                                                                        ctTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGttt            >  2:1886283/1‑148 (MQ=255)
                                                                                                                                           tACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGga         <  2:2597164/148‑1 (MQ=255)
                                                                                                                                             ccTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGgaag       >  2:1559140/1‑148 (MQ=255)
                                                                                                                                               tGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGgaagaa     <  2:3280041/148‑1 (MQ=255)
                                                                                                                                               tGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGgaagaa     <  1:2398570/148‑1 (MQ=255)
                                                                                                                                                gTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGaagaag    >  1:1768220/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GACGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAG  >  CP009273/4589927‑4590220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑