Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 394,301 C→A 15.2% S325* (TCA→TAA)  yaiW → putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273394,3010CA15.2% 488.6 / 48.6 158S325* (TCA→TAA) yaiWputative lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (63/71);  new base A (14/10);  total (77/81)
Fisher's exact test for biased strand distribution p-value = 3.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 158 total aligned reads displayed.

ACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACAGTGATCAAACAGGAATGTCAGGCCAGATA  >  CP009273/394155‑394447
                                                                                                                                                  |                                                                                                                                                  
aCTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCa                                                                                                                                                   >  1:569495/1‑148 (MQ=255)
aCTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCa                                                                                                                                                   >  1:2232252/1‑148 (MQ=255)
aCTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCa                                                                                                                                                   >  1:3074228/1‑148 (MQ=255)
 cTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCAc                                                                                                                                                  <  1:2819067/148‑1 (MQ=255)
  tGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACt                                                                                                                                                 >  2:514238/1‑148 (MQ=255)
               aCTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATg                                                                                                                                    >  1:1071303/1‑148 (MQ=255)
               aCTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATg                                                                                                                                    <  1:178358/148‑1 (MQ=255)
                cTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGt                                                                                                                                   <  1:1601472/148‑1 (MQ=255)
                cTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGt                                                                                                                                   <  2:2310475/148‑1 (MQ=255)
                cTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGt                                                                                                                                   <  1:2723528/148‑1 (MQ=255)
                 tGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGtt                                                                                                                                  <  1:2880286/148‑1 (MQ=255)
                   gCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGc                                                                                                                                <  2:2272888/148‑1 (MQ=255)
                   gCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGc                                                                                                                                <  2:611250/148‑1 (MQ=255)
                   gCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATAACTCCCCCGCGAAATGTTGc                                                                                                                                >  2:2150498/1‑148 (MQ=255)
                    cTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATAACTCCCCCGCGAAATGTTGcc                                                                                                                               <  2:2433782/148‑1 (MQ=255)
                            cTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTc                                                                                                                       >  1:946021/1‑148 (MQ=255)
                              ggggATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATAACTCCCCCGCGAAATGTTGCCTGGCATTCaa                                                                                                                     <  2:3280369/148‑1 (MQ=255)
                              ggggATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATAACTCCCCCGCGAAATGTTGCCTGGCATTCaa                                                                                                                     <  2:2380988/148‑1 (MQ=255)
                                ggATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACt                                                                                                                   <  2:270149/148‑1 (MQ=255)
                                ggATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACt                                                                                                                   >  2:623751/1‑148 (MQ=255)
                                    gAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATAACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGaa                                                                                                               >  2:2955611/1‑148 (MQ=255)
                                        gACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGcc                                                                                                           <  1:3009870/148‑1 (MQ=255)
                                        gACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGcc                                                                                                           <  1:2656607/148‑1 (MQ=255)
                                                 aTTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCa                                                                                                  >  2:2875777/1‑148 (MQ=255)
                                                  ttCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCAc                                                                                                 <  1:3147357/148‑1 (MQ=255)
                                                  ttCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCAc                                                                                                 >  2:2479408/1‑148 (MQ=255)
                                                   tCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCAcc                                                                                                <  1:2619233/148‑1 (MQ=255)
                                                   tCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCAcc                                                                                                <  2:1535173/148‑1 (MQ=255)
                                                   tCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCAcc                                                                                                >  2:2579461/1‑148 (MQ=255)
                                                   tCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCAcc                                                                                                <  1:2801101/148‑1 (MQ=255)
                                                   tCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCAcc                                                                                                <  1:2801136/148‑1 (MQ=255)
                                                   tCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGATTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCAcc                                                                                                >  2:1148563/1‑148 (MQ=255)
                                                         tCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAAc                                                                                          <  2:1467363/148‑1 (MQ=255)
                                                         tCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAAc                                                                                          >  1:885712/1‑148 (MQ=255)
                                                              tAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGAc                                                                                     >  1:2998843/1‑148 (MQ=255)
                                                                     aGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGcc                                                                              <  2:2232252/148‑1 (MQ=255)
                                                                     aGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATAACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTCCGGcc                                                                              >  1:2744365/1‑148 (MQ=255)
                                                                      ggTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCt                                                                             >  1:364823/1‑148 (MQ=255)
                                                                      ggTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCt                                                                             <  1:106455/148‑1 (MQ=255)
                                                                         gACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGt                                                                          >  1:1991090/1‑148 (MQ=255)
                                                                         gACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGt                                                                          <  2:925222/148‑1 (MQ=255)
                                                                          aCAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGtt                                                                         <  1:1524402/148‑1 (MQ=255)
                                                                            aGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTg                                                                       >  2:399710/1‑148 (MQ=255)
                                                                             gCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGc                                                                      >  2:1222948/1‑148 (MQ=255)
                                                                               ttCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGACAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGGTTGCGa                                                                    >  2:3206812/1‑148 (MQ=255)
                                                                               ttCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGa                                                                    <  1:2177705/148‑1 (MQ=255)
                                                                               ttCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGa                                                                    >  1:1935561/1‑148 (MQ=255)
                                                                               ttCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGa                                                                    >  1:1935234/1‑148 (MQ=255)
                                                                               ttCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATAACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGa                                                                    >  1:1044405/1‑148 (MQ=255)
                                                                               ttCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATAACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGa                                                                    >  1:2700989/1‑148 (MQ=255)
                                                                                 ctctTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAg                                                                  >  2:435991/1‑148 (MQ=255)
                                                                                 ctctTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATAACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAg                                                                  >  1:334853/1‑148 (MQ=255)
                                                                                        gaggagACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAg                                                           <  2:1833499/148‑1 (MQ=255)
                                                                                          ggagACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGAc                                                         >  1:775709/1‑148 (MQ=255)
                                                                                            agaCGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGa                                                       <  2:2857369/148‑1 (MQ=255)
                                                                                             gaCGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGaa                                                      <  1:1038420/148‑1 (MQ=255)
                                                                                             gaCGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGaa                                                      >  2:1207954/1‑148 (MQ=255)
                                                                                              aCGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAAc                                                     <  2:1132132/148‑1 (MQ=255)
                                                                                                ggCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAAcgg                                                   <  1:241229/148‑1 (MQ=255)
                                                                                                ggCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAAcgg                                                   <  1:2361824/148‑1 (MQ=255)
                                                                                                ggCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAAcgg                                                   <  2:672332/148‑1 (MQ=255)
                                                                                                  cgcTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAAcggcg                                                 >  2:48953/1‑148 (MQ=255)
                                                                                                  cgcTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAAcggcg                                                 <  2:2138138/148‑1 (MQ=255)
                                                                                                    cTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACggcggg                                               >  2:2718563/1‑148 (MQ=255)
                                                                                                    cTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACggcggg                                               >  1:2719386/1‑148 (MQ=255)
                                                                                                        aCAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGcgcg                                           <  1:74910/148‑1 (MQ=255)
                                                                                                             aaaGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTa                                      >  1:2740721/1‑148 (MQ=255)
                                                                                                             aaaGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTa                                      >  2:2400773/1‑148 (MQ=255)
                                                                                                             aaaGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTa                                      <  2:364823/148‑1 (MQ=255)
                                                                                                             aaaGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTa                                      <  1:532068/148‑1 (MQ=255)
                                                                                                              atgTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTAAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTAt                                     <  1:1406767/146‑1 (MQ=255)
                                                                                                                 tttATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGaa                                  <  2:672977/148‑1 (MQ=255)
                                                                                                                   tATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAAc                                >  2:3189861/1‑148 (MQ=255)
                                                                                                                    aTCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACa                               <  1:1597968/148‑1 (MQ=255)
                                                                                                                     tCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACAg                              <  1:467415/148‑1 (MQ=255)
                                                                                                                      cAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACAGt                             >  1:1820005/1‑148 (MQ=255)
                                                                                                                      cAACTTGCCGAAACGAAAACGGGTAAATAACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACAGt                             >  2:1859615/1‑148 (MQ=255)
                                                                                                                       aaCTTGCCGACACGAAAACGGGTAAATAACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACAGTg                            <  2:2013684/148‑1 (MQ=255)
                                                                                                                               gaaacgaaaACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACAGTGATCAAACa                    <  2:2541540/148‑1 (MQ=255)
                                                                                                                               gaaacgaaaACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACAGTGATCAAACa                    <  2:1366799/148‑1 (MQ=255)
                                                                                                                                aaacgaaaACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACAGTGATCAAACAg                   >  1:746200/1‑148 (MQ=255)
                                                                                                                                aaacgaaaACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACAGTGATCAAACAg                   >  1:746846/1‑148 (MQ=255)
                                                                                                                                 aacgaaaACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACAGTGATCAAACAgg                  <  2:3073564/148‑1 (MQ=255)
                                                                                                                                 aacgaaaACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACAGTGATCAAACAgg                  <  2:3074228/148‑1 (MQ=255)
                                                                                                                                 aacgaaaACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACAGTGATCAAACAgg                  >  1:1215815/1‑148 (MQ=255)
                                                                                                                                 aacgaaaACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACAGTGATCAAACAgg                  >  1:2865116/1‑148 (MQ=255)
                                                                                                                                 aacgaaaACGGGTAAATAACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACAGTGATCAAACAgg                  >  1:2154293/1‑148 (MQ=255)
                                                                                                                                  acgaaaACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACAGTGATCAAACAGGa                 >  2:105297/1‑148 (MQ=255)
                                                                                                                                  acgaaaACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACAGTGATCAAACAGGa                 >  2:2369843/1‑148 (MQ=255)
                                                                                                                                    gaaaACGGGTAAATAACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACAGTGATCAAACAGGAAt               <  2:892877/148‑1 (MQ=255)
                                                                                                                                     aaaaCGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACAGTGATCAAACAGGAATg              >  2:2100157/1‑148 (MQ=255)
                                                                                                                                        actggTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACAGTGATCAAACAGGAATGTCa           >  1:907499/4‑148 (MQ=255)
                                                                                                                                        actggTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACAGTGATCAAACAGGAATGTCa           >  1:907506/4‑148 (MQ=255)
                                                                                                                                        aCGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACAGTGATCAAACAGGAATGTCa           <  2:1967278/148‑1 (MQ=255)
                                                                                                                                          gggTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACAGTGATCAAACAGGAATGTCAgg         <  1:3189861/148‑1 (MQ=255)
                                                                                                                                           ggTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACAGTGATCAAACAGGAATGTCAGGc        >  2:417685/1‑148 (MQ=255)
                                                                                                                                              aaaTCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACAGTGATCAAACAGGAATGTCAGGCCAg     >  1:2099841/1‑148 (MQ=255)
                                                                                                                                              aaaTCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACAGTGATCAAACAGGAATGTCAGGCCAg     >  1:2099845/1‑148 (MQ=255)
                                                                                                                                                 tCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACAGTGATCAAACAGGAATGTCAGGCCAGATa  <  1:2513584/148‑1 (MQ=255)
                                                                                                                                                 tCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACAGTGATCAAACAGGAATGTCAGGCCAGATa  <  1:2513433/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACGAAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAAATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGTTGTATGAAACAGTGATCAAACAGGAATGTCAGGCCAGATA  >  CP009273/394155‑394447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑