Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 394,744 G→T 10.5% R16S (CGC→AGC)  yaiY ← DUF2755 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273394,7440GT10.5% 710.2 / 39.5 208R16S (CGC→AGC) yaiYDUF2755 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (105/81);  new base T (9/13);  total (114/94)
Fisher's exact test for biased strand distribution p-value = 1.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 214 total aligned reads displayed.

GTGGGCGACCTGTTTCCTGGACCTGCATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAA  >  CP009273/394597‑394891
                                                                                                                                                   |                                                                                                                                                   
gTGGGCGACCTGTTTCCTGGACCTGCATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCg                                                                                                                                                     <  2:986811/148‑1 (MQ=255)
gTGGGCGACCTGTTTCCTGGACCTGCATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCg                                                                                                                                                     <  2:1950911/148‑1 (MQ=255)
gTGGGCGACCTGTTTCCTGGACCTGCATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCg                                                                                                                                                     <  2:2151808/148‑1 (MQ=255)
       aCCTGTTTCCTGGACCTGCATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATAtttt                                                                                                                                              >  2:1697866/1‑148 (MQ=255)
       aCCTGTTTCCTGGACCTGCATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATAtttt                                                                                                                                              <  2:1225086/148‑1 (MQ=255)
         cTGTTTCCTGGACCTGCATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTcc                                                                                                                                            >  1:354715/1‑148 (MQ=255)
              tCCTGGACCTGCATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCTATATTTTCCGCTaa                                                                                                                                       <  2:2393092/148‑1 (MQ=255)
               ccTGGACCTGCATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTaaa                                                                                                                                      <  1:2246060/148‑1 (MQ=255)
                  ggACCTGCATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAg                                                                                                                                   <  2:134960/148‑1 (MQ=255)
                     ccTGCATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTACGCTAAACAGCGa                                                                                                                                <  1:3095265/148‑1 (MQ=255)
                     ccTGCATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTACGCTAAACAGCGa                                                                                                                                <  1:1651988/148‑1 (MQ=255)
                       tGCATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCTATATTTTCCGCTAAACAGCGAtt                                                                                                                              >  2:1435905/1‑148 (MQ=255)
                       tGCATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGAtt                                                                                                                              >  1:503547/1‑148 (MQ=255)
                       tGCATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGAtt                                                                                                                              <  1:548608/148‑1 (MQ=255)
                        gCATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGAttt                                                                                                                             <  1:876041/148‑1 (MQ=255)
                             aaaCGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACa                                                                                                                        <  2:3013531/148‑1 (MQ=255)
                               aCGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCTATATTTTCCGCTAAACAGCGATTTTGACAgg                                                                                                                      >  1:2837198/1‑148 (MQ=255)
                               aCGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAgg                                                                                                                      <  2:3117236/148‑1 (MQ=255)
                                 gCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGt                                                                                                                    >  2:2003468/1‑148 (MQ=255)
                                  cTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTg                                                                                                                   >  2:2345244/1‑148 (MQ=255)
                                    cATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGaa                                                                                                                 >  1:2546156/1‑148 (MQ=255)
                                        gACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCa                                                                                                             >  1:2124792/1‑148 (MQ=255)
                                         aCGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAg                                                                                                            >  1:2698839/1‑148 (MQ=255)
                                           gCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCTATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGcc                                                                                                          >  2:1129880/1‑148 (MQ=255)
                                           gCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGcc                                                                                                          <  1:2660802/148‑1 (MQ=255)
                                             cGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCAt                                                                                                        >  1:3138486/1‑148 (MQ=255)
                                                    aTGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAAcc                                                                                                 <  1:1780148/148‑1 (MQ=255)
                                                            aCAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAg                                                                                         >  1:3228295/1‑148 (MQ=255)
                                                            aCAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAg                                                                                         >  2:1977857/1‑148 (MQ=255)
                                                            aCAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAg                                                                                         >  1:1588624/1‑148 (MQ=255)
                                                             cAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGt                                                                                        <  1:1424612/148‑1 (MQ=255)
                                                               ggTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCa                                                                                      >  1:227473/1‑148 (MQ=255)
                                                                      cAATTGCGCCCCAGCCCAAAAGCAAAACAGCACATACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGctt                                                                               >  1:488947/1‑148 (MQ=255)
                                                                      cAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGctt                                                                               >  2:645608/1‑148 (MQ=255)
                                                                      cAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGctt                                                                               <  1:1356794/148‑1 (MQ=255)
                                                                        aTTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGcttct                                                                             <  2:29511/148‑1 (MQ=255)
                                                                          tGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGcttctct                                                                           >  1:814821/1‑148 (MQ=255)
                                                                          tGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGcttctct                                                                           <  2:2661810/148‑1 (MQ=255)
                                                                           gcgcCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGcttctctt                                                                          <  1:465313/148‑1 (MQ=255)
                                                                            cgcCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCTATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGcttctcttc                                                                         >  1:2475292/1‑148 (MQ=255)
                                                                            cgcCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGcttctcttc                                                                         >  1:1138861/1‑148 (MQ=255)
                                                                            cgcCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGcttctcttc                                                                         >  1:1139723/1‑148 (MQ=255)
                                                                             gcCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGcttctcttct                                                                        <  2:478413/148‑1 (MQ=255)
                                                                              ccccAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTg                                                                       >  2:922044/1‑148 (MQ=255)
                                                                              ccccAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTg                                                                       <  1:587902/148‑1 (MQ=255)
                                                                                         aaGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCa                                                            >  1:1623804/1‑148 (MQ=255)
                                                                                         aaGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCa                                                            >  2:2806481/1‑148 (MQ=255)
                                                                                          aGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCTATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAg                                                           >  2:284101/1‑148 (MQ=255)
                                                                                           gCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCTATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGc                                                          >  2:2730139/1‑148 (MQ=255)
                                                                                           gCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGc                                                          >  1:2862770/1‑148 (MQ=255)
                                                                                           gCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGc                                                          >  1:2352260/1‑148 (MQ=255)
                                                                                           gCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGc                                                          >  2:3252409/1‑148 (MQ=255)
                                                                                            cAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCg                                                         >  1:2465750/1‑148 (MQ=255)
                                                                                             aaaaCAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCgg                                                        >  2:1774096/1‑148 (MQ=255)
                                                                                              aaaCAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGc                                                       <  1:1393754/148‑1 (MQ=255)
                                                                                              aaaCAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGc                                                       >  1:2831172/1‑148 (MQ=255)
                                                                                               aaCAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCt                                                      >  1:2582947/1‑148 (MQ=255)
                                                                                                aCAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCtt                                                     <  2:2863297/148‑1 (MQ=255)
                                                                                                aCAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCtt                                                     >  2:1929072/1‑148 (MQ=255)
                                                                                                 cAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCTATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTa                                                    >  1:2581836/1‑148 (MQ=255)
                                                                                                  aGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTAt                                                   <  2:2466196/148‑1 (MQ=255)
                                                                                                  aGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTAt                                                   <  2:54421/148‑1 (MQ=255)
                                                                                                  aGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTAt                                                   <  2:49290/148‑1 (MQ=255)
                                                                                                   gCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATg                                                  >  2:2480007/1‑148 (MQ=255)
                                                                                                    cacaAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATgg                                                 >  1:2756666/1‑148 (MQ=255)
                                                                                                     acaaacaaCGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGc                                                >  1:612410/1‑148 (MQ=255)
                                                                                                        accaacGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAg                                             <  2:416888/146‑1 (MQ=255)
                                                                                                         acaacGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGt                                            >  1:713464/1‑148 (MQ=255)
                                                                                                         acaacGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATATGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGt                                            >  2:1364518/1‑148 (MQ=255)
                                                                                                            acGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCCTCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTg                                         >  2:1871201/1‑148 (MQ=255)
                                                                                                            acGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGAGACCAGCGGCTTATGGCAAGTCTg                                         >  2:3027074/1‑148 (MQ=255)
                                                                                                             cGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGa                                        >  1:329457/1‑148 (MQ=255)
                                                                                                                aTAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAg                                     >  1:1908997/1‑148 (MQ=255)
                                                                                                                    gCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCa                                 >  1:1028858/1‑148 (MQ=255)
                                                                                                                    gCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCa                                 <  1:1697866/148‑1 (MQ=255)
                                                                                                                    gCAATGGTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCa                                 <  2:1091307/148‑1 (MQ=255)
                                                                                                                        tGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTa                             >  2:2061281/1‑148 (MQ=255)
                                                                                                                         gTTGCCAGGCGTTGAAGAGGCATTGCTATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAg                            <  1:1231075/148‑1 (MQ=255)
                                                                                                                           tGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGAt                          <  1:645615/148‑1 (MQ=255)
                                                                                                                            gCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGAtt                         >  1:1877659/1‑148 (MQ=255)
                                                                                                                            gCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGAtt                         >  1:2022088/1‑148 (MQ=255)
                                                                                                                             ccAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTg                        <  1:1544865/148‑1 (MQ=255)
                                                                                                                                 gCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCaa                    <  2:2842682/148‑1 (MQ=255)
                                                                                                                                   gTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAata                  <  2:2249321/148‑1 (MQ=255)
                                                                                                                                    ttGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAatat                 <  1:1201427/148‑1 (MQ=255)
                                                                                                                                     tGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATc                >  1:992337/1‑148 (MQ=255)
                                                                                                                                       aaGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAg              >  1:2247612/1‑148 (MQ=255)
                                                                                                                                        agagGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGa             <  2:1565062/148‑1 (MQ=255)
                                                                                                                                        agagGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGa             >  1:2952893/1‑148 (MQ=255)
                                                                                                                                        agagGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGa             >  1:2996274/1‑148 (MQ=255)
                                                                                                                                        agagGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGa             <  2:695886/148‑1 (MQ=255)
                                                                                                                                         gagGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGaa            <  2:381155/148‑1 (MQ=255)
                                                                                                                                         gagGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGaa            <  2:264706/148‑1 (MQ=255)
                                                                                                                                         gagGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGaa            <  1:3150520/148‑1 (MQ=255)
                                                                                                                                              aTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATg       <  1:2443843/148‑1 (MQ=255)
                                                                                                                                              aTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATg       >  2:589815/1‑148 (MQ=255)
                                                                                                                                               ttGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATgg      <  1:1310436/148‑1 (MQ=255)
                                                                                                                                               ttGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATgg      >  1:3280744/1‑148 (MQ=255)
                                                                                                                                                   gATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTaaa  <  2:1588624/148‑1 (MQ=255)
                                                                                                                                                   gATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTaaa  <  2:3228295/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GTGGGCGACCTGTTTCCTGGACCTGCATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAA  >  CP009273/394597‑394891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑