Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 396,883 A→G 100% Q14Q (CAA→CAG)  iraP → anti‑RssB factor, RpoS stabilzer during Pi starvation; anti‑adapter protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273396,8830AG100.0% 856.1 / NA 209Q14Q (CAA→CAG) iraPanti‑RssB factor, RpoS stabilzer during Pi starvation; anti‑adapter protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (109/100);  total (109/100)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 210 total aligned reads displayed.

TTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGA  >  CP009273/396738‑397026
                                                                                                                                                 |                                                                                                                                               
ttGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCagaa                                                                                                                                               >  1:1824077/1‑148 (MQ=255)
       aaGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTTTGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAaagaagaa                                                                                                                                        <  2:1580703/148‑1 (MQ=255)
        aGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAaagaagaag                                                                                                                                       <  2:2538733/148‑1 (MQ=255)
          tATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAagag                                                                                                                                     <  1:1611466/148‑1 (MQ=255)
           atgtCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGt                                                                                                                                    <  2:2667798/145‑1 (MQ=255)
             ttCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCg                                                                                                                                  >  2:823097/1‑148 (MQ=255)
             ttCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCg                                                                                                                                  >  2:2910178/1‑148 (MQ=255)
               ccTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGACAGACGAAGAGTCGaa                                                                                                                                >  2:2997366/1‑148 (MQ=255)
                     taaTAAAAATAATACTTCCAGACACTATGAATTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACt                                                                                                                          <  1:3106136/146‑1 (MQ=255)
                        tAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACtgtg                                                                                                                       >  1:2972272/1‑148 (MQ=255)
                         aaaaaTAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACtgtgt                                                                                                                      <  1:624493/148‑1 (MQ=255)
                         aaaaaTAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACtgtgt                                                                                                                      >  2:1762606/1‑148 (MQ=255)
                            aataatACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTgcg                                                                                                                   >  2:1399286/1‑148 (MQ=255)
                             ataataCTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTgcgc                                                                                                                  <  1:3296349/148‑1 (MQ=255)
                               aataCTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGGGTGCGCAg                                                                                                                >  2:2038441/1‑148 (MQ=255)
                                ataCTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAgg                                                                                                               >  1:1013393/1‑148 (MQ=255)
                                     tCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGaa                                                                                                          >  1:2346733/1‑148 (MQ=255)
                                      ccAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAg                                                                                                         <  2:2346733/148‑1 (MQ=255)
                                       cAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGc                                                                                                        <  1:2680939/148‑1 (MQ=255)
                                        aGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCt                                                                                                       <  2:1039453/148‑1 (MQ=255)
                                        aGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCt                                                                                                       <  2:1039651/148‑1 (MQ=255)
                                         gACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCtt                                                                                                      <  1:2301567/148‑1 (MQ=255)
                                          acacTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCttt                                                                                                     >  1:2121088/1‑148 (MQ=255)
                                          acacTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCttt                                                                                                     >  1:2353993/1‑148 (MQ=255)
                                          acacTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCttt                                                                                                     >  1:2817188/1‑148 (MQ=255)
                                           cacTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTg                                                                                                    <  1:1364502/148‑1 (MQ=255)
                                             cTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGga                                                                                                  >  1:1812012/1‑148 (MQ=255)
                                              tATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGgag                                                                                                 >  1:3208951/1‑148 (MQ=255)
                                               aTGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGgaga                                                                                                <  1:1700403/148‑1 (MQ=255)
                                               aTGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGgaga                                                                                                <  1:2392077/148‑1 (MQ=255)
                                               aTGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGgaga                                                                                                >  2:1098978/1‑148 (MQ=255)
                                                 gAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGAtt                                                                                              <  1:998955/148‑1 (MQ=255)
                                                  aaGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTa                                                                                             <  1:1399752/148‑1 (MQ=255)
                                                   aGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTAt                                                                                            <  1:2601843/148‑1 (MQ=255)
                                                    gTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATc                                                                                           <  1:509602/148‑1 (MQ=255)
                                                        tgAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCa                                                                                       >  1:1432368/1‑148 (MQ=255)
                                                            aCATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGc                                                                                   <  1:1957707/148‑1 (MQ=255)
                                                             cATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCa                                                                                  <  1:902258/148‑1 (MQ=255)
                                                              aTAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCaa                                                                                 >  2:2837784/1‑148 (MQ=255)
                                                              aTAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCaa                                                                                 >  2:1591474/1‑148 (MQ=255)
                                                              aTAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCaa                                                                                 <  1:3212446/148‑1 (MQ=255)
                                                                aaTGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATg                                                                               >  2:781185/1‑148 (MQ=255)
                                                                aaTGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATg                                                                               >  2:2751193/1‑148 (MQ=255)
                                                                aaTGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATg                                                                               <  1:111709/148‑1 (MQ=255)
                                                                aaTGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATg                                                                               <  1:1248771/148‑1 (MQ=255)
                                                                  tGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCt                                                                             >  1:2918301/1‑148 (MQ=255)
                                                                   gTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCtt                                                                            <  1:2792995/148‑1 (MQ=255)
                                                                    ttAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTc                                                                           >  2:3147561/1‑148 (MQ=255)
                                                                       aCTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCa                                                                        <  2:2957052/148‑1 (MQ=255)
                                                                       aCTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCa                                                                        <  2:1223492/148‑1 (MQ=255)
                                                                          tctcCATACTTTGGATAAGGAAATACAGACATTAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAata                                                                     >  2:2628827/1‑148 (MQ=255)
                                                                             ccATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATgg                                                                  <  1:1669056/148‑1 (MQ=255)
                                                                               aTACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGgcg                                                                >  1:2862582/1‑148 (MQ=255)
                                                                                 aCTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCa                                                              >  2:42205/1‑148 (MQ=255)
                                                                                    ttGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCaaaa                                                           <  1:1755886/148‑1 (MQ=255)
                                                                                       gATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGa                                                        >  1:1974263/1‑148 (MQ=255)
                                                                                       gATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGa                                                        >  1:2998498/1‑148 (MQ=255)
                                                                                        aTAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGAc                                                       >  1:3245986/1‑148 (MQ=255)
                                                                                         tAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGAcc                                                      >  2:1435497/1‑148 (MQ=255)
                                                                                         tAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGAcc                                                      >  1:546009/1‑148 (MQ=255)
                                                                                           aGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCaa                                                    <  1:3171772/148‑1 (MQ=255)
                                                                                            ggAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAAc                                                   >  2:910937/1‑148 (MQ=255)
                                                                                             gAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACa                                                  >  2:721545/1‑148 (MQ=255)
                                                                                                aTACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCg                                               >  1:3196651/1‑148 (MQ=255)
                                                                                                  aCAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGTAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGt                                             >  1:1879952/1‑148 (MQ=255)
                                                                                                  aCAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGt                                             <  1:2936412/148‑1 (MQ=255)
                                                                                                  aCAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGt                                             >  2:2232292/1‑148 (MQ=255)
                                                                                                   cAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGtt                                            <  2:2124992/148‑1 (MQ=255)
                                                                                                    aGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttg                                           >  2:2465882/1‑148 (MQ=255)
                                                                                                      aCATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttgat                                         >  2:462782/1‑148 (MQ=255)
                                                                                                          gAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTtgattgat                                     >  1:1269267/1‑148 (MQ=255)
                                                                                                          gAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTtgattgat                                     >  1:1269018/1‑148 (MQ=255)
                                                                                                          gAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTtgattgat                                     <  2:1661795/148‑1 (MQ=255)
                                                                                                           aaaaaTCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATc                                    <  2:1951075/148‑1 (MQ=255)
                                                                                                             aaaTCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAg                                  <  2:2353993/148‑1 (MQ=255)
                                                                                                              aaTCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAgg                                 <  1:429855/148‑1 (MQ=255)
                                                                                                                 ctcATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTag                              >  2:559124/1‑148 (MQ=255)
                                                                                                                 ctcATTGCTGAGGTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTag                              <  1:1727409/148‑1 (MQ=255)
                                                                                                                  tcATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTaga                             >  2:738348/1‑148 (MQ=255)
                                                                                                                  tcATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTaga                             <  1:2180810/148‑1 (MQ=255)
                                                                                                                   cATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTagag                            >  2:865805/1‑148 (MQ=255)
                                                                                                                   cATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTagag                            >  2:2807533/1‑148 (MQ=255)
                                                                                                                   cATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTagag                            <  1:2362610/148‑1 (MQ=255)
                                                                                                                   cATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTagag                            <  2:1824077/148‑1 (MQ=255)
                                                                                                                        cTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGgc                       >  1:2270577/1‑148 (MQ=255)
                                                                                                                           aGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCt                    >  2:3184240/1‑148 (MQ=255)
                                                                                                                           aGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGAGTGATCAGGTAGAGGGGGCGCt                    >  2:1741149/1‑148 (MQ=255)
                                                                                                                             ttgttATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAGGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGt                  >  1:1684842/1‑148 (MQ=255)
                                                                                                                              tgttATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTa                 >  1:1461252/1‑148 (MQ=255)
                                                                                                                                ttATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACg               >  2:1307564/1‑148 (MQ=255)
                                                                                                                                 tATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGa              >  2:667661/1‑148 (MQ=255)
                                                                                                                                 tATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGa              >  2:941405/1‑148 (MQ=255)
                                                                                                                                   tttAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAgg            >  2:1356445/1‑148 (MQ=255)
                                                                                                                                        gCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTCAAg       >  2:2029357/1‑148 (MQ=255)
                                                                                                                                        gCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAg       >  2:362868/1‑148 (MQ=255)
                                                                                                                                         cTTGCCCAGAAAGAAGAAGAGTCGAAAGATCTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGc      <  1:3018171/148‑1 (MQ=255)
                                                                                                                                         cTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGc      >  2:3025807/1‑148 (MQ=255)
                                                                                                                                         cTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGc      >  2:188427/1‑148 (MQ=255)
                                                                                                                                            gCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCg   <  2:2191460/148‑1 (MQ=255)
                                                                                                                                            gCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCg   <  2:869607/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGA  >  CP009273/396738‑397026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑