Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 400,594 G→T 88.7% V169V (GTC→GTA)  proC ← pyrroline‑5‑carboxylate reductase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273400,5940GT88.7% 407.2 / 50.4 168V169V (GTC→GTA) proCpyrroline‑5‑carboxylate reductase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base G (9/10);  new base T (68/81);  total (77/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 168 total aligned reads displayed.

TTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTG  >  CP009273/400449‑400738
                                                                                                                                                 |                                                                                                                                                
tttCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTac                                                                                                                                                >  2:2733167/1‑148 (MQ=255)
tttCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTac                                                                                                                                                >  1:31623/1‑148 (MQ=255)
 ttCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGTCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTacc                                                                                                                                               >  1:380782/1‑148 (MQ=255)
 ttCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTacc                                                                                                                                               <  1:1933633/148‑1 (MQ=255)
 ttCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTacc                                                                                                                                               <  1:263498/148‑1 (MQ=255)
 ttCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGACCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTacc                                                                                                                                               >  1:2137408/1‑148 (MQ=255)
       cACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCggg                                                                                                                                         <  1:628189/148‑1 (MQ=255)
       cACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCggg                                                                                                                                         >  2:2006823/1‑148 (MQ=255)
         ccATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTg                                                                                                                                       <  1:3102716/148‑1 (MQ=255)
           aTTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGa                                                                                                                                     <  1:2076362/148‑1 (MQ=255)
               ttGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGatcat                                                                                                                                 <  2:2556565/148‑1 (MQ=255)
                 gCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCg                                                                                                                               >  1:343266/1‑148 (MQ=255)
                 gCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCg                                                                                                                               >  1:1296317/1‑148 (MQ=255)
                 gCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCg                                                                                                                               >  1:1296316/1‑148 (MQ=255)
                   ggAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGc                                                                                                                             >  2:3244127/1‑148 (MQ=255)
                    gAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGTCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCt                                                                                                                            <  2:380782/148‑1 (MQ=255)
                    gAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCt                                                                                                                            >  1:490238/1‑148 (MQ=255)
                       cccATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAg                                                                                                                         >  1:2533914/1‑148 (MQ=255)
                            tACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAAtt                                                                                                                    >  2:735456/1‑148 (MQ=255)
                             aCCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTa                                                                                                                   <  1:1575253/148‑1 (MQ=255)
                             aCCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTa                                                                                                                   <  2:1717983/148‑1 (MQ=255)
                             aCCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTa                                                                                                                   <  1:1575269/148‑1 (MQ=255)
                              ccGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTAc                                                                                                                  >  1:1784809/1‑148 (MQ=255)
                               cGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACt                                                                                                                 <  1:460412/148‑1 (MQ=255)
                                gCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACtt                                                                                                                <  2:2408593/148‑1 (MQ=255)
                                   tGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCg                                                                                                             <  2:453428/148‑1 (MQ=255)
                                     aGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCt                                                                                                           >  1:52474/1‑148 (MQ=255)
                                     aGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCt                                                                                                           >  1:402912/1‑148 (MQ=255)
                                         gCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGc                                                                                                       <  1:658289/148‑1 (MQ=255)
                                         gCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGc                                                                                                       <  1:2319097/148‑1 (MQ=255)
                                          cAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGcc                                                                                                      >  1:1469041/1‑148 (MQ=255)
                                             aTTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCaaa                                                                                                   >  2:310031/1‑148 (MQ=255)
                                             aTTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCaaa                                                                                                   >  2:310052/1‑148 (MQ=255)
                                               ttATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAagc                                                                                                 <  1:460138/148‑1 (MQ=255)
                                               ttATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAagc                                                                                                 <  2:27442/148‑1 (MQ=255)
                                                 ataCGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAagcag                                                                                               <  2:1498189/148‑1 (MQ=255)
                                                  taCGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAagcagc                                                                                              >  1:261926/1‑148 (MQ=255)
                                                  taCGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAagcagc                                                                                              <  1:826773/148‑1 (MQ=255)
                                                   aCGCCTGGGCGCGTGGCATCCCGCCCAGCACGTCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCg                                                                                             >  1:756669/1‑148 (MQ=255)
                                                    cGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCgg                                                                                            >  1:2328983/1‑148 (MQ=255)
                                                        tGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAACTACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGaaaa                                                                                        <  2:1349786/148‑1 (MQ=255)
                                                        tGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGaaaa                                                                                        <  1:2964280/148‑1 (MQ=255)
                                                         gggCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAat                                                                                       >  2:1189715/1‑148 (MQ=255)
                                                             gcgTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTc                                                                                   <  2:2346830/148‑1 (MQ=255)
                                                                          cccACCACGGCGGCGTCGGCCATCGCTTCGATAAACAGAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTACGCCAAAGCAGCGGAAAATATTCAGCACATCAGCgg                                                                      <  2:224168/148‑1 (MQ=255)
                                                                           ccAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGt                                                                     <  1:1570388/148‑1 (MQ=255)
                                                                           ccAGCACGGCGACGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGt                                                                     <  1:2485103/148‑1 (MQ=255)
                                                                            cAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTa                                                                    >  2:2689111/1‑148 (MQ=255)
                                                                            cAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTa                                                                    >  2:921173/1‑148 (MQ=255)
                                                                             aGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTAt                                                                   <  1:2726612/148‑1 (MQ=255)
                                                                               cACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTAtct                                                                 >  2:433489/1‑148 (MQ=255)
                                                                                aCGGCGGCGTCGGCCATCGCTTCGATAAACATACATACGTAGGCTGGCGAAGAACCGCTCACCCCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTAtctt                                                                <  2:1333032/148‑1 (MQ=255)
                                                                                  ggcggcGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTAtcttct                                                              <  1:2605822/148‑1 (MQ=255)
                                                                                         tCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTa                                                       >  2:851947/1‑148 (MQ=255)
                                                                                         tCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTa                                                       <  1:267101/148‑1 (MQ=255)
                                                                                             ccATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAg                                                   <  2:1955874/148‑1 (MQ=255)
                                                                                                tCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAgcgc                                                >  1:1956608/1‑148 (MQ=255)
                                                                                                tCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAgcgc                                                >  1:287845/1‑148 (MQ=255)
                                                                                                 cGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGcgcg                                               <  1:2351825/148‑1 (MQ=255)
                                                                                                   cTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCTCCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGtt                                             <  2:2899094/148‑1 (MQ=255)
                                                                                                    ttCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGttt                                            >  2:1086813/1‑148 (MQ=255)
                                                                                                     tCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTg                                           >  1:1009723/1‑148 (MQ=255)
                                                                                                     tCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTg                                           >  1:789911/1‑148 (MQ=255)
                                                                                                     tCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTg                                           >  1:771041/1‑148 (MQ=255)
                                                                                                     tCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTg                                           >  2:3048218/1‑148 (MQ=255)
                                                                                                     tCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTg                                           <  1:2515489/148‑1 (MQ=255)
                                                                                                     tCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTg                                           >  2:804970/1‑148 (MQ=255)
                                                                                                     tCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTg                                           <  1:2515455/148‑1 (MQ=255)
                                                                                                       gATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGc                                         >  2:1282670/1‑148 (MQ=255)
                                                                                                         tAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGt                                       <  2:68878/148‑1 (MQ=255)
                                                                                                         tAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGt                                       >  1:186208/1‑148 (MQ=255)
                                                                                                           aaCATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTa                                     <  1:2770841/148‑1 (MQ=255)
                                                                                                           aaCATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTa                                     <  1:2771092/148‑1 (MQ=255)
                                                                                                           aaCATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTa                                     <  1:2771156/148‑1 (MQ=255)
                                                                                                              aTAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACgg                                  >  1:1005374/1‑148 (MQ=255)
                                                                                                              aTAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACgg                                  <  2:2576225/148‑1 (MQ=255)
                                                                                                              aTAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACgg                                  <  1:1400801/148‑1 (MQ=255)
                                                                                                                aaaTACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAg                                >  1:3056195/1‑148 (MQ=255)
                                                                                                                aaaTACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAg                                <  2:1942155/148‑1 (MQ=255)
                                                                                                                aaaTACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAg                                <  2:2068298/148‑1 (MQ=255)
                                                                                                                aaaTACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAg                                >  2:2975658/1‑148 (MQ=255)
                                                                                                                  aTACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGt                              >  2:1632016/1‑148 (MQ=255)
                                                                                                                  aTACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGt                              >  2:2055528/1‑148 (MQ=255)
                                                                                                                   tACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTc                             >  2:518056/1‑148 (MQ=255)
                                                                                                                     cGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCAt                           <  1:2467780/148‑1 (MQ=255)
                                                                                                                       tAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATcc                         <  2:2994846/148‑1 (MQ=255)
                                                                                                                          gCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCgg                      >  1:1683644/1‑148 (MQ=255)
                                                                                                                             ggCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCAt                   >  1:1458391/1‑148 (MQ=255)
                                                                                                                                 aagaaCCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAAc               >  1:2229570/1‑148 (MQ=255)
                                                                                                                                    aaCCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAg            >  2:546919/1‑148 (MQ=255)
                                                                                                                                    aaCCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAg            >  2:2209812/1‑148 (MQ=255)
                                                                                                                                     aCCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGACAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGt           >  2:2058267/1‑148 (MQ=255)
                                                                                                                                      ccGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTg          >  2:286202/1‑148 (MQ=255)
                                                                                                                                        gCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCg        >  1:1104361/1‑148 (MQ=255)
                                                                                                                                           cacaCCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGa     >  1:3312350/1‑148 (MQ=255)
                                                                                                                                            acacCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAg    >  2:167857/1‑148 (MQ=255)
                                                                                                                                            acacCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAg    <  1:3217908/148‑1 (MQ=255)
                                                                                                                                             cacCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAgt   >  1:1325126/1‑148 (MQ=255)
                                                                                                                                             cacCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAgt   >  2:2892567/1‑148 (MQ=255)
                                                                                                                                              acCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAgtg  <  2:2336992/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTG  >  CP009273/400449‑400738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑