BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	403,157	G→T	7.6%	D92Y (GAC→TAC)	aroM →	AroM family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	403,157	0	G	T	7.6%	772.0 / 24.9	210	D92Y (GAC→TAC)	aroM	AroM family protein
Reads supporting (aligned to +/- strand): ref base G (92/102); new base T (9/7); total (101/109)
Fisher's exact test for biased strand distribution p-value = 6.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 213 total aligned reads displayed.

AAGAAGTGATGGCGGAGTACGCGCCAGAAGCAGGCGAAGACACCATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGTGGGGGTTATCGTTCCGGTTGAGGAGA  >  CP009273/403011‑403304

aagaagTGATGGCGGAGTACGTGCCAGAAGCAGGCGAAGACACCATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGa                                                                                                                                                    <  1:633599/148‑1 (MQ=255)

aagaagTGATGGCGGAGTACGCGCCAGAAGCAGGCGAAGACACCATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGa                                                                                                                                                    <  1:1991498/148‑1 (MQ=255)

 agaagTGATGGCGGAGTACGCGCCAGAAGCAGGCGAAGACACCATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGAc                                                                                                                                                   >  1:2232201/1‑148 (MQ=255)

     gTGATGGCGGAGTACGCGCCAGAAGCAGGCGAAGACACCATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCa                                                                                                                                               >  2:3166454/1‑148 (MQ=255)

       gATGGCGGAGTACGCGCCAGAAGCAGGCGAAGACACCATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCAtt                                                                                                                                             <  1:1816344/148‑1 (MQ=255)

        aTGGCGGAGTACGCGCCAGAAGCAGGCGAAGACACCATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCAttt                                                                                                                                            <  1:1268913/148‑1 (MQ=255)

         tGGCGGAGTACGCGCCAGAAGCAGGCGAAGACACCATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCAtttt                                                                                                                                           >  1:475451/1‑148 (MQ=255)

          ggCGGAGTACGCGCCAGAAGCAGGCGAAGACACCATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTtta                                                                                                                                          <  1:2417782/148‑1 (MQ=255)

           gCGGAGTACGCGCCAGAAGCAGGCGAAGACACCATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTttat                                                                                                                                         <  2:556636/148‑1 (MQ=255)

             ggAGTACGCGCCAGAAGCAGGCGAAGACACCATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTttatta                                                                                                                                       <  1:2923706/148‑1 (MQ=255)

             ggAGTACGCGCCAGAAGCAGGCGAAGACACCATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTttatta                                                                                                                                       <  1:2899234/148‑1 (MQ=255)

              gAGTACGCGCCAGAAGCAGGCGAAGACACCATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTaa                                                                                                                                      >  2:13168/1‑148 (MQ=255)

                 tACGCGCCAGAAGCAGGCGAAGACACCATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGa                                                                                                                                   >  1:1897878/1‑148 (MQ=255)

                   cgcgCCAGAAGCAGGCGAAGACACCATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGt                                                                                                                                 <  2:2268151/148‑1 (MQ=255)

                      gcCAGAAGCAGGCGAAGACACCATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACa                                                                                                                              <  1:647185/148‑1 (MQ=255)

                        cAGAAGCAGGCGAAGACACCAGTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGc                                                                                                                            <  2:2072965/148‑1 (MQ=255)

                          gAAGCAGGCGAAGACACCATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCaa                                                                                                                          >  2:1800000/1‑148 (MQ=255)

                          gAAGCAGGCGAAGACACCATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCaa                                                                                                                          >  1:2796371/1‑148 (MQ=255)

                          gAAGCAGGCGAAGACACCATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCaa                                                                                                                          <  1:1332532/148‑1 (MQ=255)

                             gCAGGCGAAGACACCATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACa                                                                                                                       <  2:2232201/148‑1 (MQ=255)

                               aGGCGAAGACACCATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACAtt                                                                                                                     <  2:446537/148‑1 (MQ=255)

                                    aaGACACCATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATtagtag                                                                                                                >  2:3280045/1‑148 (MQ=255)

                                     aGACACCATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTagtagt                                                                                                               <  2:2643393/148‑1 (MQ=255)

                                      gACACCATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATTACGTCATTTTATTAATGAGTACAGCAAACATTAgtagta                                                                                                              <  1:956586/148‑1 (MQ=255)

                                       acacCATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTAt                                                                                                             >  1:1328986/1‑148 (MQ=255)

                                       acacCATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTAt                                                                                                             >  1:230144/1‑148 (MQ=255)

                                       acacCATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTAt                                                                                                             >  1:1529500/1‑148 (MQ=255)

                                          ccATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATACTCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGAc                                                                                                          >  2:3308892/1‑148 (MQ=255)

                                          ccATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATTACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGAc                                                                                                          >  1:846276/1‑148 (MQ=255)

                                              tctcACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTgcg                                                                                                      >  2:2935820/1‑148 (MQ=255)

                                                    attattAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAAt                                                                                                <  1:2350662/148‑1 (MQ=255)

                                                    attattAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAAt                                                                                                >  1:2465412/1‑148 (MQ=255)

                                                     ttattaAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATTACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATa                                                                                               >  1:1188043/1‑148 (MQ=255)

                                                     ttattaAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATa                                                                                               <  2:2901516/148‑1 (MQ=255)

                                                      tattaAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATAc                                                                                              >  1:1393237/1‑148 (MQ=255)

                                                      tattaAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATAc                                                                                              >  2:2293471/1‑148 (MQ=255)

                                                       attaAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATTACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACg                                                                                             <  2:2315123/148‑1 (MQ=255)

                                                       attaAATGACAACCAGCTGGCCCATGTTTCGCGTCACAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACg                                                                                             >  2:742473/1‑148 (MQ=255)

                                                         taAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGAt                                                                                           <  1:1584233/148‑1 (MQ=255)

                                                         taAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGAt                                                                                           <  2:1332919/148‑1 (MQ=255)

                                                            aTGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGAtctt                                                                                        >  1:2574764/1‑148 (MQ=255)

                                                             tGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGAtcttt                                                                                       <  1:2024738/148‑1 (MQ=255)

                                                             tGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGAtcttt                                                                                       <  2:1391474/148‑1 (MQ=255)

                                                              gACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGAtctttc                                                                                      <  2:2830854/148‑1 (MQ=255)

                                                               aCAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGAtctttct                                                                                     >  1:2888439/1‑148 (MQ=255)

                                                                 aaCCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTg                                                                                   <  1:2137214/148‑1 (MQ=255)

                                                                       cTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGt                                                                             >  1:508897/1‑148 (MQ=255)

                                                                         ggCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTcg                                                                           >  2:3309651/1‑148 (MQ=255)

                                                                         ggCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTcg                                                                           <  2:1148921/148‑1 (MQ=255)

                                                                                gTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATTACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAatat                                                                    >  1:66896/1‑148 (MQ=255)

                                                                                  ttCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTg                                                                  <  1:2289441/148‑1 (MQ=255)

                                                                                  ttCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTg                                                                  >  2:3059939/1‑148 (MQ=255)

                                                                                   tCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGc                                                                 >  1:857176/1‑148 (MQ=255)

                                                                                      cgTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTc                                                              <  2:3170898/148‑1 (MQ=255)

                                                                                           cAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTg                                                         >  1:955313/1‑148 (MQ=255)

                                                                                            aaaGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTgg                                                        <  1:1361497/148‑1 (MQ=255)

                                                                                            aaaGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTgg                                                        <  1:3009296/148‑1 (MQ=255)

                                                                                              aGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGtt                                                      <  1:726112/148‑1 (MQ=255)

                                                                                                 ggAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTcc                                                   >  2:159418/1‑148 (MQ=255)

                                                                                                 ggAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTcc                                                   <  1:482520/148‑1 (MQ=255)

                                                                                                 ggAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTcc                                                   >  2:3198477/1‑148 (MQ=255)

                                                                                                 ggAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTcc                                                   >  2:3198257/1‑148 (MQ=255)

                                                                                                  gAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCct                                                  <  1:1008378/148‑1 (MQ=255)

                                                                                                  gAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCct                                                  <  1:3229076/148‑1 (MQ=255)

                                                                                                       tGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTAtt                                             <  2:531623/148‑1 (MQ=255)

                                                                                                           cTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTAttgttg                                         >  1:338461/1‑148 (MQ=255)

                                                                                                            tGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGa                                        >  1:2161695/1‑148 (MQ=255)

                                                                                                            tGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGa                                        <  1:1418794/148‑1 (MQ=255)

                                                                                                              cAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTATTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTTGTTTCCTCTATTGTTGAAg                                      <  2:667065/148‑1 (MQ=255)

                                                                                                              cAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAg                                      >  1:2093837/1‑148 (MQ=255)

                                                                                                                aGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATATTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGat                                    <  2:715181/148‑1 (MQ=255)

                                                                                                                 ggTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGatc                                   <  1:1421247/148‑1 (MQ=255)

                                                                                                                  gtgtGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGatca                                  >  2:390651/1‑148 (MQ=255)

                                                                                                                        ttGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGt                            <  1:796077/148‑1 (MQ=255)

                                                                                                                         tGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGTg                           >  1:1322162/1‑148 (MQ=255)

                                                                                                                          gAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGTgg                          <  2:1479894/148‑1 (MQ=255)

                                                                                                                            aGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGTgggg                        >  1:303675/1‑148 (MQ=255)

                                                                                                                             gTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGTggggg                       >  2:3233037/1‑148 (MQ=255)

                                                                                                                              tGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTTAAGATCATCAGGTGGGGGt                      >  2:1985669/1‑148 (MQ=255)

                                                                                                                               gCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGTGGGGGtt                     >  1:343966/1‑148 (MQ=255)

                                                                                                                                cTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGTTTTCCTCTATTGTTGAAGATCATCAGGTGGGGGTTa                    >  2:2485597/1‑148 (MQ=255)

                                                                                                                                cTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGTGGGGGTTa                    >  2:2485505/1‑148 (MQ=255)

                                                                                                                                 tCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGTGGGGGTTAt                   <  2:1950867/148‑1 (MQ=255)

                                                                                                                                   gATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGTGGGGGTTATCg                 <  2:1629205/148‑1 (MQ=255)

                                                                                                                                     tAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGTGGGGGTTATCGtt               <  1:409710/148‑1 (MQ=255)

                                                                                                                                        tCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGTGGGGGTTATCGTTCCg            >  1:261996/1‑148 (MQ=255)

                                                                                                                                         cAGGGTTATTACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGTGGGGGTTATCGTTCCgg           >  1:1058932/1‑148 (MQ=255)

                                                                                                                                         cAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGTGGGGGTTATCGTTCCgg           <  2:1241107/148‑1 (MQ=255)

                                                                                                                                         cAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGTGGGGGTTATCGTTCCgg           >  1:2784289/1‑148 (MQ=255)

                                                                                                                                         cAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGTGGGGGTTATCGTTCCgg           <  2:857176/148‑1 (MQ=255)

                                                                                                                                          aGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGTGGGGGTTATCGTTCCGGt          <  1:395732/148‑1 (MQ=255)

                                                                                                                                           gggTTATGACTTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGTGGGGGTTATCGTTCCGGtt         >  1:357450/1‑148 (MQ=255)

                                                                                                                                            ggTTATTACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGTGGGGGTTATCGTTCCGGTTg        <  1:3234289/148‑1 (MQ=255)

                                                                                                                                            ggTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGTGGGGGTTATCGTTCCGGTTg        >  1:1611798/1‑148 (MQ=255)

                                                                                                                                             gTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGTGGGGGTTATCGTTCCGGTTga       >  2:272666/1‑148 (MQ=255)

                                                                                                                                              ttATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGTGGGGGTTATCGTTCCGGTTgag      >  2:2574207/1‑148 (MQ=255)

                                                                                                                                               tATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGTGGGGGTTATCGTTCCGGTTgagg     >  2:1810271/1‑148 (MQ=255)

                                                                                                                                                 tGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGTGGGGGTTATCGTTCCTGTTgaggag   <  2:2318113/148‑1 (MQ=255)

                                                                                                                                                  gACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGTGGGGGTTATCGTTCCGGTTGAGgaga  >  2:2206783/1‑148 (MQ=255)

                                                                                                                                                  gACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGTGGGGGTTATCGTTCCGGTTGAGgaga  >  1:1313234/1‑148 (MQ=255)

AAGAAGTGATGGCGGAGTACGCGCCAGAAGCAGGCGAAGACACCATTCTCACATTATTAAATGACAACCAGCTGGCCCATGTTTCGCGTCGCAAAGTGGAGCGTGACCTGCAAGGTGTGGTTGAAGTGCTCGATAATCAGGGTTATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGTGGGGGTTATCGTTCCGGTTGAGGAGA  >  CP009273/403011‑403304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`