Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 411,027 G→T 100% L61L (CTC→CTA)  sbcC ← exonuclease, dsDNA, ATP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273411,0270GT99.4% 536.0 / ‑4.5 166L61L (CTC→CTA) sbcCexonuclease, dsDNA, ATP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (74/91);  minor base A (1/0);  total (75/91)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 167 total aligned reads displayed.

TACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAA  >  CP009273/410888‑411170
                                                                                                                                           |                                                                                                                                               
   cGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGt                                                                                                                                      <  2:1115775/148‑1 (MQ=255)
   cGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGt                                                                                                                                      <  2:2370373/148‑1 (MQ=255)
    gTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGtt                                                                                                                                     <  1:2567575/148‑1 (MQ=255)
    gTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGtt                                                                                                                                     <  2:1295836/148‑1 (MQ=255)
     tCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGttt                                                                                                                                    >  2:2134014/1‑148 (MQ=255)
         gttggttACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTg                                                                                                                                <  1:2146349/148‑1 (MQ=255)
         gttggttACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTg                                                                                                                                <  1:2145594/148‑1 (MQ=255)
          ttggttACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGa                                                                                                                               >  2:1138928/1‑148 (MQ=255)
            ggttACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATa                                                                                                                             <  2:786369/148‑1 (MQ=255)
             gttACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATAc                                                                                                                            >  2:2023051/1‑148 (MQ=255)
              ttACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACa                                                                                                                           >  2:1033296/1‑148 (MQ=255)
              ttACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACa                                                                                                                           >  1:2134716/1‑148 (MQ=255)
               tACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAg                                                                                                                          <  2:3250169/148‑1 (MQ=255)
                aCGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAgc                                                                                                                         >  2:3090254/1‑148 (MQ=255)
                 cgcgCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAgcg                                                                                                                        >  2:1089676/1‑148 (MQ=255)
                 cgcgCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAgcg                                                                                                                        >  1:2738260/1‑148 (MQ=255)
                 cgcgCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAgcg                                                                                                                        >  2:770657/1‑148 (MQ=255)
                   cgcCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGcc                                                                                                                      >  2:695492/1‑148 (MQ=255)
                    gcCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCa                                                                                                                     <  2:2765198/148‑1 (MQ=255)
                         aTTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACaa                                                                                                                <  1:1001199/148‑1 (MQ=255)
                          taatgccTCCATAAACCACGCTAACCTTCACCTTTCACTTCAAACTCCACAACAGCAAGACATACCGCTGAAACGCGGGTCAATAGAACAATTAGCGATTGACAACCGATAGAAACACGCCGAGAATCGAGATACAGAGCCAGACaaa                                                                                                               >  2:258223/7‑148 (MQ=255)
                           tcgggCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAAt                                                                                                              <  2:1181802/145‑1 (MQ=255)
                           tCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAAt                                                                                                              <  1:633573/148‑1 (MQ=255)
                             tGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATgg                                                                                                            <  1:1269666/148‑1 (MQ=255)
                             tGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATgg                                                                                                            <  1:1270472/148‑1 (MQ=255)
                             tGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATgg                                                                                                            <  1:352498/148‑1 (MQ=255)
                              ggCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGc                                                                                                           >  2:1873478/1‑148 (MQ=255)
                                 tCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTc                                                                                                        <  1:669209/148‑1 (MQ=255)
                                     gAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCcagc                                                                                                    >  2:611095/1‑148 (MQ=255)
                                      aaTGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCcagca                                                                                                   >  2:3074582/1‑148 (MQ=255)
                                      aaTGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCcagca                                                                                                   >  1:413870/1‑148 (MQ=255)
                                            cGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGggtgg                                                                                             <  1:1762297/148‑1 (MQ=255)
                                            cGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGggtgg                                                                                             <  1:2768222/148‑1 (MQ=255)
                                            cGGTAAGCTTCACCTTTCAGTTCAAACTCCACATCCGCCAGACTTTCGGCGGTATCGCGGGTCATTAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGAGTCCAGCAGggtgg                                                                                             <  1:2768242/148‑1 (MQ=255)
                                             ggTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGggtggt                                                                                            <  1:1903431/148‑1 (MQ=255)
                                               tACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGttt                                                                                          <  2:217250/148‑1 (MQ=255)
                                               tACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGttt                                                                                          <  2:217413/148‑1 (MQ=255)
                                                   cTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTccc                                                                                      <  1:1327980/148‑1 (MQ=255)
                                                         cTTTCACTTCAAACTCCGCCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCAcc                                                                                >  1:189256/1‑148 (MQ=255)
                                                           ttCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTg                                                                              >  1:922928/1‑148 (MQ=255)
                                                            tCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCAGCACCTGt                                                                             <  2:2517018/148‑1 (MQ=255)
                                                             cACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGtt                                                                            <  2:2671372/148‑1 (MQ=255)
                                                             cACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGtt                                                                            <  2:2671310/148‑1 (MQ=255)
                                                               cTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTgg                                                                          >  2:3210533/1‑148 (MQ=255)
                                                                    aaCTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCgg                                                                     >  2:2111102/1‑148 (MQ=255)
                                                                    aaCTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCgg                                                                     >  1:1624262/1‑148 (MQ=255)
                                                                     aCTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGt                                                                    >  2:451436/1‑148 (MQ=255)
                                                                       tCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTaa                                                                  <  1:2413789/148‑1 (MQ=255)
                                                                        ccACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAAt                                                                 >  1:2862625/1‑148 (MQ=255)
                                                                          aCCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCAATTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGAGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAg                                                               >  2:2238488/1‑148 (MQ=255)
                                                                            cTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGGTACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCa                                                             <  1:2256743/148‑1 (MQ=255)
                                                                                gCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACa                                                         >  2:2997899/1‑148 (MQ=255)
                                                                                  cAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGc                                                       >  2:2075190/1‑148 (MQ=255)
                                                                                  cAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGc                                                       <  2:407306/148‑1 (MQ=255)
                                                                                    gACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGccc                                                     >  2:2758123/1‑148 (MQ=255)
                                                                                     aCATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCg                                                    <  1:1089676/148‑1 (MQ=255)
                                                                                     aCATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCg                                                    <  1:770657/148‑1 (MQ=255)
                                                                                        ttCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTg                                                 >  1:796564/1‑148 (MQ=255)
                                                                                        ttCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTg                                                 <  1:2346270/148‑1 (MQ=255)
                                                                                        ttCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTg                                                 <  1:414025/148‑1 (MQ=255)
                                                                                         tCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGc                                                >  1:3031567/1‑148 (MQ=255)
                                                                                         tCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGc                                                >  1:13636/1‑148 (MQ=255)
                                                                                            gcggTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTgg                                             >  2:1208743/1‑148 (MQ=255)
                                                                                                 aTCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAAc                                        >  1:1246253/1‑148 (MQ=255)
                                                                                                  tCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACg                                       <  2:1359478/148‑1 (MQ=255)
                                                                                                   cgcgGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACgg                                      >  2:204072/1‑148 (MQ=255)
                                                                                                     cgGGTCATGAGTTCATTTTGCTATTGTGAAACGTTAGATCTACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCt                                    <  1:989135/148‑1 (MQ=255)
                                                                                                     cgGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAACTGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCt                                    <  1:24273/148‑1 (MQ=255)
                                                                                                       ggTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTcg                                  >  2:2442748/1‑148 (MQ=255)
                                                                                                         tCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTcgcg                                >  1:65485/1‑148 (MQ=255)
                                                                                                         tCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTcgcg                                >  2:2334348/1‑148 (MQ=255)
                                                                                                          cATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCgg                               >  1:464150/1‑148 (MQ=255)
                                                                                                          cATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCgg                               <  2:2810393/148‑1 (MQ=255)
                                                                                                           aTGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCggg                              <  1:3308825/148‑1 (MQ=255)
                                                                                                            tGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGt                             >  2:1754093/1‑148 (MQ=255)
                                                                                                              agaTCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGa                           >  1:2491149/1‑148 (MQ=255)
                                                                                                               gaTCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGaa                          <  1:32358/148‑1 (MQ=255)
                                                                                                               gaTCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGaa                          <  2:1099855/148‑1 (MQ=255)
                                                                                                                 tCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAaat                        <  1:3248856/148‑1 (MQ=255)
                                                                                                                   aTTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAaatca                      <  1:894390/148‑1 (MQ=255)
                                                                                                                   aTTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAaatca                      <  1:3174980/148‑1 (MQ=255)
                                                                                                                     tttGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAaatcaat                    >  2:2663199/1‑148 (MQ=255)
                                                                                                                     tttGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAaatcaat                    >  2:1923185/1‑148 (MQ=255)
                                                                                                                      ttGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAaatcaatc                   >  2:251857/1‑148 (MQ=255)
                                                                                                                       tGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCt                  <  1:1585063/148‑1 (MQ=255)
                                                                                                                       tGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCt                  >  2:3263616/1‑148 (MQ=255)
                                                                                                                        gCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCtt                 <  1:1427377/148‑1 (MQ=255)
                                                                                                                         cGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTc                <  2:1447113/148‑1 (MQ=255)
                                                                                                                          gATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTcc               >  2:2111257/1‑148 (MQ=255)
                                                                                                                           aTTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCa              >  2:2980519/1‑148 (MQ=255)
                                                                                                                            ttGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCAt             >  2:2879817/1‑148 (MQ=255)
                                                                                                                             tgtgAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCAtt            <  2:982851/148‑1 (MQ=255)
                                                                                                                             tgtgAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCAtt            >  1:261511/1‑148 (MQ=255)
                                                                                                                                  aaCGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCt       >  1:699817/1‑148 (MQ=255)
                                                                                                                                  aaCGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCt       <  1:1184565/148‑1 (MQ=255)
                                                                                                                                  aaCGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCt       >  2:895023/1‑148 (MQ=255)
                                                                                                                                    cGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCttt     <  1:2303996/148‑1 (MQ=255)
                                                                                                                                      ttAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTa   <  1:246163/148‑1 (MQ=255)
                                                                                                                                      ttAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTa   <  1:3237523/148‑1 (MQ=255)
                                                                                                                                       tagatagaCGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTaa  >  1:306528/1‑148 (MQ=255)
                                                                                                                                           |                                                                                                                                               
TACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAA  >  CP009273/410888‑411170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑