Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 426,157 G→T 81.7% E33* (GAG→TAG)  yajD → HNH nuclease family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273426,1570GT81.7% 287.6 / 55.2 173E33* (GAG→TAG) yajDHNH nuclease family protein
Reads supporting (aligned to +/- strand):  ref base G (23/11);  new base T (76/63);  total (99/74)
Fisher's exact test for biased strand distribution p-value = 1.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 175 total aligned reads displayed.

CATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAG  >  CP009273/426012‑426303
                                                                                                                                                 |                                                                                                                                                  
 aTCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAgt                                                                                                                                                 <  2:2425250/148‑1 (MQ=255)
    ttGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAgtttg                                                                                                                                              <  1:2290119/148‑1 (MQ=255)
     tGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAgtttgt                                                                                                                                             <  1:773635/148‑1 (MQ=255)
       ggAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAgtttgttt                                                                                                                                           >  2:475159/1‑148 (MQ=255)
       ggAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAgtttgttt                                                                                                                                           <  1:87546/148‑1 (MQ=255)
         aaaCTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTAt                                                                                                                                         <  1:544481/148‑1 (MQ=255)
           aCTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAGAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTc                                                                                                                                       <  2:2741842/148‑1 (MQ=255)
           aCTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTc                                                                                                                                       <  2:1089528/148‑1 (MQ=255)
                   tAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGc                                                                                                                               >  2:3184229/1‑148 (MQ=255)
                      cccTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTg                                                                                                                            >  2:2964267/1‑148 (MQ=255)
                      cccTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTg                                                                                                                            >  2:1112768/1‑148 (MQ=255)
                       ccTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGa                                                                                                                           >  2:660273/1‑148 (MQ=255)
                       ccTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGa                                                                                                                           >  2:660386/1‑148 (MQ=255)
                        cTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGaa                                                                                                                          >  1:2281685/1‑148 (MQ=255)
                         tACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAAc                                                                                                                         >  1:394192/1‑148 (MQ=255)
                          aCATTTCCTCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTGAGACAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACt                                                                                                                        <  1:2958328/148‑1 (MQ=255)
                           cATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACtt                                                                                                                       <  2:1536186/148‑1 (MQ=255)
                           cATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACtt                                                                                                                       <  2:728606/148‑1 (MQ=255)
                             tttCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTAc                                                                                                                     <  1:3132141/148‑1 (MQ=255)
                             tttCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGGTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTAc                                                                                                                     <  1:3132151/148‑1 (MQ=255)
                              ttCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTAcc                                                                                                                    >  2:2619858/1‑148 (MQ=255)
                                 aTCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTACAAACTGCGTGAACTTACCGtt                                                                                                                 >  1:319740/1‑148 (MQ=255)
                                   ccAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTca                                                                                                               >  2:2945909/1‑148 (MQ=255)
                                     aGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTcacc                                                                                                             >  2:2655315/1‑148 (MQ=255)
                                       gTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTcaccac                                                                                                           >  2:2868172/1‑148 (MQ=255)
                                       gTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGGTTGTTTATTCCAACCTGCGTGAACTTACCGTTcaccac                                                                                                           >  1:803852/1‑148 (MQ=255)
                                       gTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTACCGCTAGTTTTGTTATTCCAACCAGCGTGAACTAACCGTTcaccac                                                                                                           >  2:2770460/1‑148 (MQ=255)
                                         aaCTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTGCCGCTAGTTTTTTTATTCCAACCTGCGTGTAATTAACGTTAACCACAt                                                                                                         >  1:2513651/1‑148 (MQ=255)
                                          aCTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACAtt                                                                                                        >  1:2214298/1‑148 (MQ=255)
                                           cTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAGATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTg                                                                                                       <  2:94065/148‑1 (MQ=255)
                                            ttttCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGa                                                                                                      <  1:2146871/148‑1 (MQ=255)
                                            ttttCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGa                                                                                                      <  2:33361/148‑1 (MQ=255)
                                            ttttCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGa                                                                                                      <  1:1778942/148‑1 (MQ=255)
                                             tttCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTCTCGCGAAAATGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGAt                                                                                                     >  2:1716514/1‑148 (MQ=255)
                                             tttCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGAt                                                                                                     >  1:2791566/1‑148 (MQ=255)
                                             tttCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGAt                                                                                                     >  2:1716549/1‑148 (MQ=255)
                                             tttCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCTGCGTGAACTTACCGTTCACCACATTGAt                                                                                                     >  2:1716530/1‑148 (MQ=255)
                                              ttCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAACGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATc                                                                                                    <  2:25691/148‑1 (MQ=255)
                                               tCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCa                                                                                                   <  2:75709/148‑1 (MQ=255)
                                                 aTGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACg                                                                                                 <  2:926875/148‑1 (MQ=255)
                                                 aTGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACg                                                                                                 <  2:1391175/148‑1 (MQ=255)
                                                    gCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGAcc                                                                                              >  1:2430942/1‑148 (MQ=255)
                                                      tATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCAt                                                                                            >  2:2377853/1‑148 (MQ=255)
                                                         catcCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATAcc                                                                                         >  2:1316188/1‑148 (MQ=255)
                                                            cccAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTGCCGCTAGTTTGTTTATTCCAAACTGCGTGAACTTAACGTTCACCACATTTTTAACGACCAAACCAAt                                                                                      >  1:1432673/1‑148 (MQ=255)
                                                                aaaaaCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAAcc                                                                                  <  1:2655315/148‑1 (MQ=255)
                                                                aaaaaCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGACTGAACTTACCCTTCACCACATTGATAACGACCATACCAAAAAcc                                                                                  >  2:2589995/1‑148 (MQ=255)
                                                                aaaaaCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAAcc                                                                                  >  2:2589911/1‑148 (MQ=255)
                                                                   aaCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCgg                                                                               >  2:1853161/1‑148 (MQ=255)
                                                                     cTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTACCGCTAGTTTGTTTATTCCAACCAGCGTGAACTTACAGTTCACAACATTGATCACCACCATAACAATAACCCGGaa                                                                             >  2:585359/1‑148 (MQ=255)
                                                                      tATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAg                                                                            <  2:1776800/148‑1 (MQ=255)
                                                                      tATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAg                                                                            <  2:851707/148‑1 (MQ=255)
                                                                       aTGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAAAAAACCCGAAGa                                                                           >  1:3298048/1‑148 (MQ=255)
                                                                           gcgGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGc                                                                       <  1:661959/148‑1 (MQ=255)
                                                                               ttAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTa                                                                   <  1:2308228/148‑1 (MQ=255)
                                                                               ttAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTa                                                                   <  1:3236964/148‑1 (MQ=255)
                                                                                tAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTaa                                                                  <  1:1112748/148‑1 (MQ=255)
                                                                                tAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTaa                                                                  <  1:1112768/148‑1 (MQ=255)
                                                                                  gAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAAcc                                                                                  >  1:1618519/1‑130 (MQ=255)
                                                                                   aaaGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTg                                                               >  1:2442278/1‑148 (MQ=255)
                                                                                   aaaGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTg                                                               >  1:3217693/1‑148 (MQ=255)
                                                                                     aGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGGTTGTTTATTACAACCTGCGTGAACTTACCCTTCACCACAATTATAACCACCAAACCAATAACCCGGAACATGGCAGTaaaaggg                                                             >  1:2330611/1‑143 (MQ=255)
                                                                                       cGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGaa                                                           <  2:1180720/148‑1 (MQ=255)
                                                                                        ggCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAAt                                                          <  1:1592163/148‑1 (MQ=255)
                                                                                            aTCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAAttgtt                                                      <  2:3104702/148‑1 (MQ=255)
                                                                                             tCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAAttgttg                                                     <  1:3193856/148‑1 (MQ=255)
                                                                                              cgcgAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCCGAAGATGGCCGGAACTGGGAATtgttgt                                                    >  1:1742868/1‑148 (MQ=255)
                                                                                               gcgAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAAAAACCCCGAAGATGGCAGTAACTGGGAATTGTtgtg                                                   >  1:817668/1‑148 (MQ=255)
                                                                                                cgAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTGCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCCTACCAATAACCCGGAAGTTGGCCGTAACTGGGCATTGTTgtgt                                                  >  1:1856184/1‑148 (MQ=255)
                                                                                                  aaaaaGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGtct                                                <  2:472788/148‑1 (MQ=255)
                                                                                                   aaaaGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGtctc                                               >  2:2003116/1‑148 (MQ=255)
                                                                                                    aaaGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACAATGATCACGACCATACCAATAAACCGGAAGATGGCAGTAACTGGGAAATGTTGTGTctct                                              >  1:499687/1‑148 (MQ=255)
                                                                                                     aaGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTa                                             <  2:1605845/148‑1 (MQ=255)
                                                                                                          ttAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGcc                                        >  2:2381080/1‑148 (MQ=255)
                                                                                                          ttAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGcc                                        >  2:541595/1‑148 (MQ=255)
                                                                                                           tAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGGTTGTTTATTACAACCTGCGTGAACATACCGTTCACCACATTGATCACCACCATACCAATAAACCGGAAGATTGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCa                                       >  1:1280647/1‑148 (MQ=255)
                                                                                                              aaaTCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACGTACCGTTCACCACATTGTTCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGa                                    >  1:499163/1‑148 (MQ=255)
                                                                                                                 tCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCa                                 >  2:1637056/1‑148 (MQ=255)
                                                                                                                 tCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCa                                 <  2:3005513/148‑1 (MQ=255)
                                                                                                                 tCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCa                                 <  1:234801/148‑1 (MQ=255)
                                                                                                                     tCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTTCCACGATCATGAg                             >  2:2681738/1‑148 (MQ=255)
                                                                                                                      ccGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCCATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGc                            >  2:2988699/1‑148 (MQ=255)
                                                                                                                         tGGGTCTGCGGTCTCTGTTCCCGCTCGTTTGTTTATGCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCAtt                         <  1:2009916/148‑1 (MQ=255)
                                                                                                                          gggTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTc                        >  2:1874857/1‑148 (MQ=255)
                                                                                                                             tCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGaa                     <  1:1883288/148‑1 (MQ=255)
                                                                                                                              cTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGaaa                    <  1:216707/148‑1 (MQ=255)
                                                                                                                                      gCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGa            >  1:2091481/1‑148 (MQ=255)
                                                                                                                                       cTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGaa           >  2:824532/1‑148 (MQ=255)
                                                                                                                                       cTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGaa           >  1:2114919/1‑148 (MQ=255)
                                                                                                                                         gTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGc         <  1:27992/148‑1 (MQ=255)
                                                                                                                                         gTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGc         <  1:181387/148‑1 (MQ=255)
                                                                                                                                         gTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGc         <  1:27993/148‑1 (MQ=255)
                                                                                                                                         gTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGc         >  2:2274745/1‑148 (MQ=255)
                                                                                                                                          ttCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCg        >  2:1180382/1‑148 (MQ=255)
                                                                                                                                           tCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCgg       <  1:985040/148‑1 (MQ=255)
                                                                                                                                           tCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCgg       <  1:985063/148‑1 (MQ=255)
                                                                                                                                           tCCCGCGAGTTTGATTATTCCAAACTGCGTGAACTTACCGTTCACCACTTTGATCACGACGATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCTTTCGAAATATACCGAAGCgg       <  2:2068553/148‑1 (MQ=255)
                                                                                                                                             ccGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCAGACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGAt     <  1:3069786/148‑1 (MQ=255)
                                                                                                                                              cgctAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATc    <  1:2188865/148‑1 (MQ=255)
                                                                                                                                                cgAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAg  <  2:3256026/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAG  >  CP009273/426012‑426303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑