Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 435,862 A→T 88.5% T232T (ACT→ACA)  ispA ← geranyltranstransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273435,8620AT88.5% 463.5 / 40.2 180T232T (ACT→ACA) ispAgeranyltranstransferase
Reads supporting (aligned to +/- strand):  ref base A (15/7);  new base T (79/75);  total (98/82)
Fisher's exact test for biased strand distribution p-value = 1.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 180 total aligned reads displayed.

ACTGTTCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGC  >  CP009273/435717‑436008
                                                                                                                                                 |                                                                                                                                                  
aCTGTTCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGt                                                                                                                                                  <  1:409626/148‑1 (MQ=255)
  tGTTCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTAt                                                                                                                                                <  2:2734259/148‑1 (MQ=255)
     tCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTACGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTAACAAGTTGATGGTCGGCACCCTGGCGTTTTCACAAAGTTGCTGTAtcac                                                                                                                                             >  2:1757591/1‑146 (MQ=255)
        gCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTTCTTTTCCCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCcca                                                                                                                                          <  2:2189054/148‑1 (MQ=255)
        gCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCcca                                                                                                                                          <  2:2189057/148‑1 (MQ=255)
        gCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCcca                                                                                                                                          <  2:215726/148‑1 (MQ=255)
                   cAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCAGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAgg                                                                                                                               <  1:1117546/148‑1 (MQ=255)
                     gCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCCACGTTGCTGTATCTCCCACCACATCCAGGAt                                                                                                                             >  2:961212/1‑148 (MQ=255)
                      cGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATg                                                                                                                            <  1:22265/148‑1 (MQ=255)
                        aCTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTc                                                                                                                          <  1:1958554/148‑1 (MQ=255)
                        aCTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCCGGCGTGCTCAAGACACAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGGTTGTCGGCACCCTGGCGTTTTCACCACCGTGCAGTATAAAACAACACAACCAGGAAGta                                                                                                                          >  1:3170217/1‑147 (MQ=255)
                           gACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATc                                                                                                                       <  2:1779330/148‑1 (MQ=255)
                            aCGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATcc                                                                                                                      >  2:198508/1‑148 (MQ=255)
                              gggCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACCTTGCTGTATCTCCCACCACATCCCCGATGTCATccgg                                                                                                                    >  1:1061134/1‑146 (MQ=255)
                                    cgtcgATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTg                                                                                                              <  1:3075653/148‑1 (MQ=255)
                                    cgtcgATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTACCACCACATCCAGGATGTCATCCTGAACCTg                                                                                                              <  1:3127841/148‑1 (MQ=255)
                                              aTCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGaaggcaagg                                                                                                    >  1:1671438/1‑148 (MQ=255)
                                               tCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTACCACCACATCCAGGATGTCATCCTGAACCTGGaatgcaaggc                                                                                                   >  1:2991346/1‑148 (MQ=255)
                                                cccGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACCTTGCTGTATCTACCACCCCATCCAGGATGTCATCCTGAACCAGGAAGGCAAGGcc                                                                                                  >  1:1557778/1‑148 (MQ=255)
                                                 ccGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCg                                                                                                 >  1:2709796/1‑148 (MQ=255)
                                                 ccGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTACCACCACAAACAGGATGTCATCCTGAACCTGGAAGGCAAGGCCg                                                                                                 >  1:3174602/1‑148 (MQ=255)
                                                  cGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTACCAACGTTGCGGTATCTCCCCCCACAACCAGGATGTCATCCGGAACCAGGAAGGCAAGGCCGa                                                                                                >  1:127206/1‑148 (MQ=255)
                                                   gggCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGAt                                                                                               >  2:254046/1‑148 (MQ=255)
                                                   gggCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGAt                                                                                               >  2:152459/1‑148 (MQ=255)
                                                      cTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCGAGTTGCTGGTCGGCACCCTGGCGTGTTCCCCACGGTGCTGTCTCTCCCACCCCATCCAGGCTGTCATCCTGAACCTGGGCGGCAAGGCCgctgct                                                                                            >  1:226184/1‑148 (MQ=255)
                                                      cTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGct                                                                                            <  2:1557525/148‑1 (MQ=255)
                                                      cTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGct                                                                                            <  2:1909487/148‑1 (MQ=255)
                                                       tttCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGctc                                                                                           <  1:3004751/148‑1 (MQ=255)
                                                        ttcttcCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGctct                                                                                          >  1:2811503/1‑148 (MQ=255)
                                                         tcttcCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGctctc                                                                                         >  2:1237487/1‑148 (MQ=255)
                                                         tcttcCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTACCAACGTTGCTGTATCTACCACCACATCCACGAAGTCATCCTGAACCTGGAAGGCAAGGCCGATGctctc                                                                                         >  2:2887771/1‑148 (MQ=255)
                                                            tcCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGc                                                                                      <  2:1161232/148‑1 (MQ=255)
                                                             ccGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTGCCAACGGTGCGGTATATACCACCACAACCACGATGTCATCCTGAACCTGGAAGGCAACGCCGATGCTCTCTGCa                                                                                     >  1:1119365/1‑148 (MQ=255)
                                                               gggCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATa                                                                                   <  1:1243008/148‑1 (MQ=255)
                                                                    tGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGt                                                                              <  1:2421976/148‑1 (MQ=255)
                                                                    tGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGt                                                                              >  2:1103756/1‑148 (MQ=255)
                                                                          aGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTGCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTa                                                                        >  1:812442/1‑148 (MQ=255)
                                                                           gACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCCACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATAATTGTCGAGTAc                                                                       >  2:937744/1‑148 (MQ=255)
                                                                            aCCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTAcc                                                                      >  2:2411983/1‑148 (MQ=255)
                                                                               cAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGc                                                                   <  1:2274931/148‑1 (MQ=255)
                                                                               cAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGc                                                                   <  1:612821/148‑1 (MQ=255)
                                                                               cAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATACTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGc                                                                   >  1:2810677/1‑148 (MQ=255)
                                                                               cAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTGTTCCCAACGTTGCTGTATCTCCCCCCACAACCACGATGTCATCCTGCACCTGGAAGGCAAGGCCGATGCTCTCGGCCTACTAGTCGCGTACCCGc                                                                   >  1:2420509/1‑148 (MQ=255)
                                                                                 gAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCag                                                                 >  2:3220243/1‑148 (MQ=255)
                                                                                   aGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCagag                                                               >  1:1889882/1‑148 (MQ=255)
                                                                                    gTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGc                                                              <  1:2493021/148‑1 (MQ=255)
                                                                                     tGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCa                                                             <  2:2028484/148‑1 (MQ=255)
                                                                                     tGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCa                                                             <  1:1847856/148‑1 (MQ=255)
                                                                                        aGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCacga                                                          <  1:2164522/148‑1 (MQ=255)
                                                                                          ggtaggtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATATCCCACCACAACCAGGATGTCAACCTGAACCAGGAAGGCAAGGCCCATGCTCTCTGCAAACTTGTCGAGTACCGGCAGAGCacgacg                                                        >  1:596738/1‑148 (MQ=255)
                                                                                           gtaggtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGGGTACCGGCAGAGCACGACGt                                                       >  1:2735309/1‑148 (MQ=255)
                                                                                            taggtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTc                                                      <  1:668687/148‑1 (MQ=255)
                                                                                            taggtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTc                                                      >  2:505837/1‑148 (MQ=255)
                                                                                             aggtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTcc                                                     <  2:1443017/148‑1 (MQ=255)
                                                                                             aggtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTcc                                                     <  2:3085579/148‑1 (MQ=255)
                                                                                               gtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCtt                                                   >  1:2970909/1‑148 (MQ=255)
                                                                                               gtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTACCACCACATCCAGGATGTCATACAGAACCAGGAAGGCAAGGCCCAATCTCTCTGCAAACTTGTCGGGTACCGGCACAGCACGACGTCCtt                                                   >  2:570556/1‑148 (MQ=255)
                                                                                               gtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGGTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCtt                                                   <  2:509932/148‑1 (MQ=255)
                                                                                                taCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCttt                                                  >  1:1916076/1‑148 (MQ=255)
                                                                                                taCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCttt                                                  <  2:3150405/148‑1 (MQ=255)
                                                                                                 aCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTa                                                 <  1:1160470/148‑1 (MQ=255)
                                                                                                 aCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTa                                                 <  1:1160800/148‑1 (MQ=255)
                                                                                                    tttACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCCGAGCACGACGTCCTGTAtct                                              >  2:799136/1‑148 (MQ=255)
                                                                                                    tttACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTAtct                                              >  2:3290066/1‑148 (MQ=255)
                                                                                                    tttACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTAtct                                              <  2:1462064/148‑1 (MQ=255)
                                                                                                    tttACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTAtct                                              >  2:1936022/1‑148 (MQ=255)
                                                                                                    tttACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCACTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTAtct                                              >  2:799128/1‑148 (MQ=255)
                                                                                                     ttACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCCATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTAtctc                                             <  1:1710179/148‑1 (MQ=255)
                                                                                                       aCCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCg                                           >  2:1760781/1‑148 (MQ=255)
                                                                                                        ccAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCgg                                          >  1:1078762/1‑148 (MQ=255)
                                                                                                            gTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCCGGATGTCATCCTGAAACTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGTGTAACGGCAGAGCACGACGGCCTTTATCTCCGGCGCt                                      >  2:1732584/1‑148 (MQ=255)
                                                                                                            gTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCt                                      <  2:1267792/148‑1 (MQ=255)
                                                                                                             ttGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCAGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCtt                                     >  2:572777/1‑148 (MQ=255)
                                                                                                               gCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGTATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTaa                                   <  2:2263973/148‑1 (MQ=255)
                                                                                                                  ggTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGc                                <  2:127206/148‑1 (MQ=255)
                                                                                                                       gCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCaa                           <  1:351190/148‑1 (MQ=255)
                                                                                                                         aCCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAgg                         <  1:2857017/148‑1 (MQ=255)
                                                                                                                            cTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGa                      <  2:2975723/148‑1 (MQ=255)
                                                                                                                            cTGGAGTTTTCCCAACGTTGCTGTATCTCCCAACACATCAAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTTCATACTTGTCGAGTACAGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGa                      <  1:1470643/148‑1 (MQ=255)
                                                                                                                             tGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGaa                     >  1:2102736/1‑148 (MQ=255)
                                                                                                                             tGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGaa                     >  2:1019005/1‑148 (MQ=255)
                                                                                                                              ggCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAc                    >  1:972522/1‑148 (MQ=255)
                                                                                                                                cGTTTTCCCATCGTTGCTGTATCTCCCAACACATCCAGGATGCCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATATCCGGCGCTTAATGCACCAAGGCGAAcgg                  <  1:1757591/148‑1 (MQ=255)
                                                                                                                                cGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAcgg                  <  1:2879443/148‑1 (MQ=255)
                                                                                                                                  ttgtCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAcggcg                <  2:419682/145‑1 (MQ=255)
                                                                                                                                   tttCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAcggcgg               >  2:983890/1‑148 (MQ=255)
                                                                                                                                   tttCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAcggcgg               >  1:485309/1‑148 (MQ=255)
                                                                                                                                    ttCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACggcggc              >  2:3121962/1‑148 (MQ=255)
                                                                                                                                    ttCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACggcggc              >  1:624962/1‑148 (MQ=255)
                                                                                                                                     tCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGgcggcg             >  2:1978070/1‑148 (MQ=255)
                                                                                                                                        cAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGa          >  1:864079/1‑148 (MQ=255)
                                                                                                                                        cAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGa          <  2:1491329/148‑1 (MQ=255)
                                                                                                                                          aCGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAAt        >  2:694612/1‑148 (MQ=255)
                                                                                                                                           cGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTACTGCACCAAGGCGAACGGCGGCGCGAATc       >  2:1291602/1‑148 (MQ=255)
                                                                                                                                           cGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATc       >  2:2483619/1‑148 (MQ=255)
                                                                                                                                             ttGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCaa     >  1:102431/1‑148 (MQ=255)
                                                                                                                                              tGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAAt    <  2:1133668/148‑1 (MQ=255)
                                                                                                                                              tGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAAt    >  1:1365252/1‑148 (MQ=255)
                                                                                                                                              tGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAAt    >  1:202658/1‑148 (MQ=255)
                                                                                                                                                cAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATgc  >  2:2090641/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
ACTGTTCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGC  >  CP009273/435717‑436008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑