Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 450,290 G→A 100% intergenic (+45/‑299) bolA → / → tig stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273450,2900GA100.0% 532.7 / ‑6.7 162intergenic (+45/‑299)bolA/tigstationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (70/91);  minor base T (0/1);  total (70/92)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 163 total aligned reads displayed.

TGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAAT‑TGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGC  >  CP009273/450146‑450434
                                                                                                                                                |                                                                                                                                                   
tGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtt                                                                                                                                                  <  1:180370/148‑1 (MQ=255)
tGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtt                                                                                                                                                  <  1:180079/148‑1 (MQ=255)
  cgcTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtttt                                                                                                                                                <  1:3006077/148‑1 (MQ=255)
  cgcTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtttt                                                                                                                                                <  2:1773449/148‑1 (MQ=255)
   gcTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTa                                                                                                                                               <  1:2559374/148‑1 (MQ=255)
     tGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATg                                                                                                                                             <  2:1368067/148‑1 (MQ=255)
     tGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATg                                                                                                                                             <  2:1928002/148‑1 (MQ=255)
      ggCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTCTGTTGCTAAAAATTTTATGa                                                                                                                                            >  2:2793709/1‑148 (MQ=255)
      ggCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGa                                                                                                                                            >  2:1892614/1‑148 (MQ=255)
      ggCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGa                                                                                                                                            >  2:1892617/1‑148 (MQ=255)
      ggCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGa                                                                                                                                            <  2:3005020/148‑1 (MQ=255)
       gCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaa                                                                                                                                           >  2:2550714/1‑148 (MQ=255)
       gCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaa                                                                                                                                           <  2:2380160/148‑1 (MQ=255)
         tctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaa                                                                                                                                         >  2:2880786/1‑148 (MQ=255)
         tctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaa                                                                                                                                         <  2:2836792/148‑1 (MQ=255)
          ctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaaa                                                                                                                                        >  1:3155857/1‑148 (MQ=255)
           tGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAAc                                                                                                                                       <  2:1587999/148‑1 (MQ=255)
               tACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGcc                                                                                                                                   <  2:957311/148‑1 (MQ=255)
                aCTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCt                                                                                                                                  >  2:2870561/1‑148 (MQ=255)
                aCTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTGATGAAAAACGGCCt                                                                                                                                  >  2:3058090/1‑148 (MQ=255)
                        tATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCg                                                                                                                          >  1:1622492/1‑148 (MQ=255)
                         aTTATGTAGTGGTACGGTTTGAAGGACACCGTCTTTGCCTCTCCGCCCTGTCGTGTAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCgt                                                                                                                         <  1:3277823/148‑1 (MQ=255)
                           tAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttt                                                                                                                       >  1:767492/1‑148 (MQ=255)
                           tAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttt                                                                                                                       >  2:2289969/1‑148 (MQ=255)
                             aGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttg                                                                                                                     <  2:562053/148‑1 (MQ=255)
                              ggAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttg‑t                                                                                                                   <  2:1061648/148‑1 (MQ=255)
                              ggAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgtgttg‑t                                                                                                                   >  2:2167180/1‑148 (MQ=255)
                               gAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttg‑tt                                                                                                                  <  2:1688240/148‑1 (MQ=255)
                               gAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttg‑tt                                                                                                                  <  2:1687750/148‑1 (MQ=255)
                               gAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttg‑tt                                                                                                                  <  2:1568122/148‑1 (MQ=255)
                                  tGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttg‑ttttt                                                                                                               >  1:3035723/1‑147 (MQ=255)
                                  tGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGttttg‑ttttg                                                                                                               <  1:1396173/148‑1 (MQ=255)
                                   gggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTtttg‑ttttgt                                                                                                              <  2:3193426/148‑1 (MQ=255)
                                    ggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAATTTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTc                                                                                                             <  1:1199894/148‑1 (MQ=255)
                                      aaGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTg                                                                                                           >  2:1557924/1‑148 (MQ=255)
                                        gggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGa                                                                                                         <  2:3027764/148‑1 (MQ=255)
                                         ggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGAt                                                                                                        <  2:2403579/148‑1 (MQ=255)
                                          gTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCTCGTAAAAACGCATTTTCAACGTTCGGCGCTTTTCCTGTATGTAGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGAtt                                                                                                       <  1:2188963/148‑1 (MQ=255)
                                           ttGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAACACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGAttt                                                                                                      <  1:1555051/148‑1 (MQ=255)
                                            tGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGAtttt                                                                                                     <  2:2345144/148‑1 (MQ=255)
                                                ggACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTgcgc                                                                                                 >  2:1882925/1‑148 (MQ=255)
                                                ggACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGTCTGGATTTTgggc                                                                                                 >  2:1850750/1‑148 (MQ=255)
                                                ggACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGTCTGGATTTTgcgc                                                                                                 >  2:2198373/1‑148 (MQ=255)
                                                 gACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGGGTG‑TTTTGTGTGGATTTTGCGCt                                                                                                >  2:1488929/1‑148 (MQ=255)
                                                  acacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑GTTTTTATGGCTTTTTGGCtt                                                                                               >  1:1608678/1‑148 (MQ=255)
                                                           ttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAg                                                                                      <  1:431816/148‑1 (MQ=255)
                                                           ttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTATGGTTTTTTGGCTTTTTGcgccg                                                                                      >  2:326150/1‑144 (MQ=255)
                                                            tGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATTTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGc                                                                                     <  1:2047897/148‑1 (MQ=255)
                                                               ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAtt                                                                                  <  2:200087/148‑1 (MQ=255)
                                                               ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTTTTGGTTTTTTGGTTTTTTGcccgccat                                                                                   >  1:2798124/1‑143 (MQ=255)
                                                                tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTc                                                                                 >  2:2056576/1‑148 (MQ=255)
                                                                tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTc                                                                                 <  2:204019/148‑1 (MQ=255)
                                                                tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGGTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTc                                                                                 >  2:1248472/1‑148 (MQ=255)
                                                                    ctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑c                                                                            <  2:1456020/148‑1 (MQ=255)
                                                                     tccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTTTCTGGTTTTTTCGCTTTTTGCCCAGCAATCAGA‑Cg                                                                           >  1:3029549/1‑148 (MQ=255)
                                                                     tccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCCGCATTCAGA‑Cg                                                                           >  2:199820/1‑148 (MQ=255)
                                                                         tGTCGTGGAGCAGGAAGCCTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGaaaa                                                                       <  2:2234188/148‑1 (MQ=255)
                                                                         tGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGaaaa                                                                       <  2:1422201/148‑1 (MQ=255)
                                                                          gTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTTCGCTTTTTTCCCAGCATTCAGA‑CGaaaaa                                                                      >  2:922141/1‑147 (MQ=255)
                                                                          gTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAAt                                                                      <  2:2725134/148‑1 (MQ=255)
                                                                                 aGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTTTCTGGATTTTGCGCTTTTTTGCCAGGATTCTGA‑CGAAAATTGCCCgg                                                               >  2:1266817/1‑148 (MQ=255)
                                                                                   cAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGa                                                             <  2:2980975/148‑1 (MQ=255)
                                                                                     ggAAGCATCGCGTAAACACGCATTTGCAAGTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAAt                                                           <  2:2552980/148‑1 (MQ=255)
                                                                                     ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAAt                                                           <  1:521098/148‑1 (MQ=255)
                                                                                     ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGTGGTGATTTTGGGCTTTTTTGCCCGCATTCAGA‑CGAAAATTGCCCCGGCAt                                                           >  2:2270291/1‑148 (MQ=255)
                                                                                      gAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAAt‑t                                                         <  1:2143241/148‑1 (MQ=255)
                                                                                      gAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGGTG‑TTTTGTCTGGATTTTTCGCTTTTTGCCCAGCATTAAGA‑CGAAACTTGCCCGGGCAt‑t                                                         >  2:2615400/1‑148 (MQ=255)
                                                                                       aaGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAATTTTTATGAAACACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAAT‑tg                                                        <  1:3072153/148‑1 (MQ=255)
                                                                                       aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGTGTGGATTTTGCGCTTTTTGGCCAGCATTCAGC‑CGAAAAGTGCCCGGGGAT‑tg                                                        >  2:3132659/1‑148 (MQ=255)
                                                                                            tCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAAT‑TGTGaaa                                                   <  2:3155857/148‑1 (MQ=255)
                                                                                            tCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAAT‑TGTGaaa                                                   <  1:362957/148‑1 (MQ=255)
                                                                                              gcttAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAAT‑TGTGaaaaa                                                 <  1:2580868/145‑1 (MQ=255)
                                                                                                  aaaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGGTTGTTTTTTTTTGGTTTTTGGGTTTTTTGCCCCCCA‑TAAGACCAAAAATTCCCCGGGAATGTGTAaaaaaaa                                               >  1:2332324/1‑147 (MQ=255)
                                                                                                      aCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGCTTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAAT‑TGTGAAAAAATAAGCGa                                         >  2:1390469/1‑148 (MQ=255)
                                                                                                      aCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCCGCATTCAGA‑CGAAAATTGCCCGGGAAT‑TGTGAAAAAATACGCGa                                         >  2:1392043/1‑148 (MQ=255)
                                                                                                      aCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGTGTGGGTTTTTGGGTTTTTGGCCCCCATTAAGA‑CGAAAATTGCCCGGGGAA‑TGTGGAAAAATAcgcgg                                         >  1:1900779/1‑147 (MQ=255)
                                                                                                       cGCATTTGCAACTGTCGGCGCTTTTCCATTTTGTTGATAAAATTTTTATTAAAAACGGCCTGCGGGCCTTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAAT‑TGTGAAAAAATACGCGAc                                        <  1:441999/148‑1 (MQ=255)
                                                                                                       cGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTTTGTGGTTTTTTTGGTTTTTTGCCAGCCTTCAGG‑CGGAAAATTCCCGGGGAG‑TTTGTAAAAATACACGAc                                        >  1:1752706/1‑148 (MQ=255)
                                                                                                         cATTTGCACCTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATCAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAAT‑TGTGAAAAAATACGCGACAg                                      <  1:1326705/148‑1 (MQ=255)
                                                                                                           tttGAAACTGTCGGCGCTTTTCCATTATTTTGATAAAAATTTTTTCAAAAACGGCCTGGGGGCCTTTTTT‑TTTTGTCTGTATTTTGCGATTTTTGCCCATCATTAAGA‑CTAAAATTGCCCGGGAAT‑TGTGAAAAAATACGCGACAgcg                                    <  1:2089616/148‑1 (MQ=255)
                                                                                                                  cTGTCGGCTCTTTTCCAGTATGTTGATAAAATTTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAAT‑TGTGAAAAAATACGCGACAGCGCGCAATa                             <  1:457675/148‑1 (MQ=255)
                                                                                                                   tGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTTGCTTTTTGGCTTTTTTCCCAGCAATAAGA‑CCAAAAATTCCCGGGAAT‑TTTGTAAAAATAAGCGACAACGCGCAaaaa                            >  2:1175326/1‑148 (MQ=255)
                                                                                                                        gcgcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTTCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAAT‑TGTGAAAAAAAAAGCGACAGCGCGCAAAAACCGtt                       >  2:2699285/1‑148 (MQ=255)
                                                                                                                         cgcTTTTCCCGTAGGTGGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCGGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAAT‑TGTGAAAAAATACGCGACAGCGCGCAATAACCGTtc                      <  1:55145/148‑1 (MQ=255)
                                                                                                                         cgcTTTTCCATTTTTTTGAAAAAATTTTTATCAAAAACGGCCTGCGGGCCTTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CTAAAATTGCCCGGGAAT‑TGTGAAAAAATACGCGACAGCGCGCAATAACCGTtc                      <  1:2250192/148‑1 (MQ=255)
                                                                                                                           cTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTTTCTGGATTTTTCGCTTTTTTCCCCGCATTCAGA‑CGAAAAATGCCCGGGAAT‑TTTGAAAAAATAAGCGACATCGCGCAAAAAACGTtctc                    >  2:2864681/1‑148 (MQ=255)
                                                                                                                            ttttCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTTGATTTTGCGCTTTTTGCCCACCATTCAGA‑CGAAAATTGCCCGGGAAA‑TGTGAAAAAAAACGCGACAGCGCGCAATAAACGTTATCg                   >  2:528164/1‑148 (MQ=255)
                                                                                                                            ttttCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCACCAATAACA‑CGAAAATTGCCCGGGAAA‑TGTGAAAAAAAAAGCGGCAGCGCGCAATAACCGGTGTCg                   >  2:891542/1‑148 (MQ=255)
                                                                                                                             tgtCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAAT‑TGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGa                  <  2:436723/146‑1 (MQ=255)
                                                                                                                                cctgtttGTTGATAAAAATTTTATTAAAAACGGCCTGCGGGCGGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAATTTGCCCGGGAAT‑TGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTc               <  1:880491/142‑1 (MQ=255)
                                                                                                                                 cAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAAT‑TGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCa              <  1:77892/148‑1 (MQ=255)
                                                                                                                                  aGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTTTCTGGTTTTTTGGCTTTTTGGCCCCCCGTCAGA‑CGAAAAATGCCCCGGAAA‑TGTGAAAAAATAACCCACACCCCGCAATAACCCGTATCGACTCAt             >  1:2179495/1‑148 (MQ=255)
                                                                                                                                   gTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTTTGTTGATTTTTCGCTTTTTTCCCACCATTAAGA‑CCAAAAAAGACCCGGAAT‑TTTGAAAAAATAAAAGACAACGCGCAAAAAACGGTATAGACACATa            >  1:2967838/1‑148 (MQ=255)
                                                                                                                                       tttGCTAAATATTTTATCAAAAACGGCCGGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAAT‑TGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAg        <  1:2269186/147‑1 (MQ=255)
                                                                                                                                         tGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGGGCTTTTTTCCCAGCAATCAGA‑CGAAAATTGGCCGGGAAT‑TGTTAAAAAAAAAGCGACACCGCGCAAAAACCGTTCTCGACACATAAAAGTg      >  2:1040390/1‑148 (MQ=255)
                                                                                                                                         tGCTAAAAATTTTATCAAACACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGTAAT‑TGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTg      <  1:2020595/148‑1 (MQ=255)
                                                                                                                                          gCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAAT‑TGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGa     <  2:2332324/148‑1 (MQ=255)
                                                                                                                                            tAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAAT‑TGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATg   <  1:1898096/148‑1 (MQ=255)
                                                                                                                                            tAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAAT‑TGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATg   <  1:1088653/148‑1 (MQ=255)
                                                                                                                                             aaaaATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTTTGTGGCTTTTGGGCGTTTTTCCCAGCATTCAGA‑CGAAAATTTCCCGGGGAT‑TGTGAAAAAATAAGCGACAGCGCGCCATAACCGTTGTCGACTCAAAAAAGTTAttc  >  2:690484/1‑146 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAAT‑TGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGC  >  CP009273/450146‑450434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑