Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 7,522 G→T 83.3% F146L (TTC→TTA)  yaaJ ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092737,5220GT83.3% 348.3 / 82.1 168F146L (TTC→TTA) yaaJputative transporter
Reads supporting (aligned to +/- strand):  ref base G (15/13);  new base T (69/71);  total (84/84)
Fisher's exact test for biased strand distribution p-value = 8.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 168 total aligned reads displayed.

GAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAAC  >  CP009273/7377‑7663
                                                                                                                                                 |                                                                                                                                             
gAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTaa                                                                                                                                             >  2:484903/1‑148 (MQ=255)
     aTTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGaa                                                                                                                                        <  2:1948212/148‑1 (MQ=255)
        gCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGc                                                                                                                                     <  2:1156841/148‑1 (MQ=255)
         ccAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGcc                                                                                                                                    <  2:43364/148‑1 (MQ=255)
            gcagAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCAt                                                                                                                                 >  1:2450225/1‑148 (MQ=255)
             cagAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATc                                                                                                                                <  2:302941/148‑1 (MQ=255)
                agTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAg                                                                                                                             >  1:1459183/1‑148 (MQ=255)
                agTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAg                                                                                                                             >  2:2377503/1‑148 (MQ=255)
                  tAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAgcg                                                                                                                           >  2:520412/1‑148 (MQ=255)
                   aaaGACAGCTAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAgcgc                                                                                                                          >  1:566011/1‑148 (MQ=255)
                    aaGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCa                                                                                                                         >  1:233749/1‑148 (MQ=255)
                      gACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATc                                                                                                                       >  1:3233899/1‑148 (MQ=255)
                         aGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATcccc                                                                                                                    <  1:6641/148‑1 (MQ=255)
                           cGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAg                                                                                                                  >  2:2273590/1‑148 (MQ=255)
                              gaATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCGTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGccc                                                                                                               >  2:3197143/1‑148 (MQ=255)
                                 taatGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCcgcg                                                                                                            >  1:2790436/1‑148 (MQ=255)
                                    tGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCgcgcgc                                                                                                         >  1:1624939/1‑148 (MQ=255)
                                    tGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCgcgcgc                                                                                                         >  1:1624778/1‑148 (MQ=255)
                                    tGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCgcgcgc                                                                                                         >  1:288862/1‑148 (MQ=255)
                                        tGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCata                                                                                                     >  1:257771/1‑148 (MQ=255)
                                         gTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCatat                                                                                                    <  2:566011/148‑1 (MQ=255)
                                            aCCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATAcc                                                                                                 <  2:1761544/148‑1 (MQ=255)
                                            aCCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATAcc                                                                                                 <  1:2605348/148‑1 (MQ=255)
                                             ccAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCa                                                                                                <  1:20725/148‑1 (MQ=255)
                                               aGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATg                                                                                              <  2:1492565/148‑1 (MQ=255)
                                                    tggAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGt                                                                                         <  2:1732678/147‑1 (MQ=255)
                                                    gggAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGt                                                                                         <  2:1625594/148‑1 (MQ=255)
                                                        aaaTCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGc                                                                                     <  2:1065481/148‑1 (MQ=255)
                                                        aaaTCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGc                                                                                     <  2:1428928/148‑1 (MQ=255)
                                                        aaaTCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGc                                                                                     <  1:3102680/148‑1 (MQ=255)
                                                        aaaTCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGc                                                                                     <  1:3100629/148‑1 (MQ=255)
                                                         aaTCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGACAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGcc                                                                                    >  2:33349/1‑148 (MQ=255)
                                                           tCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCAc                                                                                  >  2:1230906/1‑148 (MQ=255)
                                                              aaaGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGaa                                                                               >  2:2985013/1‑148 (MQ=255)
                                                              aaaGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGaa                                                                               >  2:3038844/1‑148 (MQ=255)
                                                               aaGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGaaa                                                                              >  1:2281558/1‑148 (MQ=255)
                                                               aaGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGaaa                                                                              <  2:1288225/148‑1 (MQ=255)
                                                               aaGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGaaa                                                                              >  1:1462206/1‑148 (MQ=255)
                                                                 gAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACt                                                                            >  1:343681/1‑148 (MQ=255)
                                                                  aaaaaCTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTg                                                                           >  1:2195617/1‑148 (MQ=255)
                                                                       cTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCAt                                                                      >  1:1788068/1‑148 (MQ=255)
                                                                          aGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGa                                                                   <  2:23396/148‑1 (MQ=255)
                                                                            ggCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACg                                                                 <  2:2471064/148‑1 (MQ=255)
                                                                             gcgcGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGt                                                                <  1:947546/148‑1 (MQ=255)
                                                                              cgcgGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTc                                                               <  2:2966535/148‑1 (MQ=255)
                                                                              cgcgGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTc                                                               <  2:2887032/148‑1 (MQ=255)
                                                                               gcgGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCa                                                              <  2:1468173/148‑1 (MQ=255)
                                                                                 gggCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACg                                                            <  2:1459183/148‑1 (MQ=255)
                                                                                 gggCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACg                                                            <  1:2904368/148‑1 (MQ=255)
                                                                                      aCGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCtt                                                       <  1:2192599/148‑1 (MQ=255)
                                                                                        ggCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTta                                                     >  1:2026595/1‑148 (MQ=255)
                                                                                         gCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTtat                                                    <  1:3188442/148‑1 (MQ=255)
                                                                                           gTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATaa                                                  <  1:2455955/148‑1 (MQ=255)
                                                                                             tCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAg                                                >  1:1731910/1‑148 (MQ=255)
                                                                                             tCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAg                                                >  1:2645617/1‑148 (MQ=255)
                                                                                              cGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGt                                               <  1:507900/148‑1 (MQ=255)
                                                                                                 ttGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTtgt                                            >  1:2002146/1‑148 (MQ=255)
                                                                                                  tGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTtgtg                                           >  1:2704805/1‑148 (MQ=255)
                                                                                                   gAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGc                                          >  2:2410830/1‑148 (MQ=255)
                                                                                                   gAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGc                                          >  1:458049/1‑148 (MQ=255)
                                                                                                     aCTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCa                                        <  2:2281558/148‑1 (MQ=255)
                                                                                                     aCTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCa                                        >  2:3215297/1‑148 (MQ=255)
                                                                                                      cTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCag                                       <  2:535237/148‑1 (MQ=255)
                                                                                                      cTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCag                                       >  1:1291983/1‑148 (MQ=255)
                                                                                                        ccGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCagag                                     >  1:2845354/1‑148 (MQ=255)
                                                                                                         cGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAgaga                                    <  2:938289/148‑1 (MQ=255)
                                                                                                         cGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAgaga                                    >  1:2294541/1‑148 (MQ=255)
                                                                                                         cGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAgaga                                    >  1:2294573/1‑148 (MQ=255)
                                                                                                          gCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGaa                                   <  1:1209271/148‑1 (MQ=255)
                                                                                                            tGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGACATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTATTTATAAAGTTGTGCCAGAGAACa                                 <  1:68607/148‑1 (MQ=255)
                                                                                                             gAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACAt                                >  1:1702180/1‑148 (MQ=255)
                                                                                                               aaaTTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTc                              <  2:2265498/148‑1 (MQ=255)
                                                                                                                 aTTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCgg                            <  2:2450443/148‑1 (MQ=255)
                                                                                                                  ttATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGc                           >  2:2749168/1‑148 (MQ=255)
                                                                                                                  ttATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGc                           >  2:2749163/1‑148 (MQ=255)
                                                                                                                     tGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCCTTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCaaa                        <  1:1637990/148‑1 (MQ=255)
                                                                                                                      gCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAAc                       >  2:644088/1‑148 (MQ=255)
                                                                                                                      gCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAAc                       >  1:456120/1‑148 (MQ=255)
                                                                                                                        cATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGa                     <  1:1132384/148‑1 (MQ=255)
                                                                                                                        cATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAATTTGTGCCAGAGAACATTCGGCAAACGa                     <  2:1624939/148‑1 (MQ=255)
                                                                                                                        cATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAATTTGTGCCAGAGAACATTCGGCAAACGa                     <  2:1624778/148‑1 (MQ=255)
                                                                                                                           aGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGt                  >  2:1089933/1‑148 (MQ=255)
                                                                                                                           aGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGt                  >  2:1091381/1‑148 (MQ=255)
                                                                                                                               gATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGcc              <  2:1951976/148‑1 (MQ=255)
                                                                                                                               gATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGcc              <  1:155642/148‑1 (MQ=255)
                                                                                                                                aTGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCa             <  2:1368178/148‑1 (MQ=255)
                                                                                                                                aTGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCa             <  2:1368177/148‑1 (MQ=255)
                                                                                                                                aTGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCa             <  1:486487/148‑1 (MQ=255)
                                                                                                                                 tGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCAt            >  2:2059239/1‑148 (MQ=255)
                                                                                                                                  gAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCACACGAGGTCGCCATg           >  2:2167229/1‑148 (MQ=255)
                                                                                                                                  gAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATg           >  2:504935/1‑148 (MQ=255)
                                                                                                                                    gCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGcc         >  1:686683/1‑148 (MQ=255)
                                                                                                                                     cAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCg        <  1:2156104/148‑1 (MQ=255)
                                                                                                                                       aaaaGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGAt      >  1:286305/1‑148 (MQ=255)
                                                                                                                                        aaaGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGTGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATa     >  1:3228949/1‑148 (MQ=255)
                                                                                                                                         aaGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATaa    <  1:2377503/148‑1 (MQ=255)
                                                                                                                                         aaGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATaa    <  2:1788068/148‑1 (MQ=255)
                                                                                                                                          ataCGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATaaa   >  2:842538/3‑148 (MQ=255)
                                                                                                                                          aGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCACATTGACGTCACGTTCTGTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATaaa   >  2:2557001/1‑148 (MQ=255)
                                                                                                                                           gACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAAc  >  1:755354/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
GAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAAC  >  CP009273/7377‑7663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑