Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 477,267 G→T 90.4% Q865K (CAG→AAG)  acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273477,2670GT90.4% 406.3 / 38.6 156Q865K (CAG→AAG) acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (8/7);  new base T (84/57);  total (92/64)
Fisher's exact test for biased strand distribution p-value = 7.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

Only 100 of 156 total aligned reads displayed.

CACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTC  >  CP009273/477126‑477411
                                                                                                                                             |                                                                                                                                                
cACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGa                                                                                                                                            <  1:2174889/148‑1 (MQ=255)
cACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGa                                                                                                                                            <  2:2729489/148‑1 (MQ=255)
  ccGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACa                                                                                                                                          <  2:3287231/148‑1 (MQ=255)
   cGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACAt                                                                                                                                         <  1:2947429/148‑1 (MQ=255)
    gATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATc                                                                                                                                        <  1:2907804/148‑1 (MQ=255)
    gATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATc                                                                                                                                        >  2:247865/1‑148 (MQ=255)
     aTAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATcc                                                                                                                                       >  2:1211897/1‑148 (MQ=255)
      tAACCCCCAGCGGAACGACCAGCATAACGGAGAACGTAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATAGCGTACAGTGAAGGTGCATGGATGCCGGAGAGAAGATACTTATAGCACATacc                                                                                                                                      >  1:1959219/1‑148 (MQ=255)
          ccccCGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTc                                                                                                                                  <  1:25384/148‑1 (MQ=255)
          ccccAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTc                                                                                                                                  <  1:720823/148‑1 (MQ=255)
          ccccAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTc                                                                                                                                  >  2:831081/1‑148 (MQ=255)
          ccccAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTc                                                                                                                                  >  2:831540/1‑148 (MQ=255)
              aGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGt                                                                                                                              >  2:1364209/1‑148 (MQ=255)
                  gAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATa                                                                                                                          >  2:158494/1‑148 (MQ=255)
                   aaCGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAg                                                                                                                         >  2:1007111/1‑148 (MQ=255)
                   aaCGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAg                                                                                                                         >  1:43415/1‑148 (MQ=255)
                   aaCGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAg                                                                                                                         >  2:387513/1‑148 (MQ=255)
                     cGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGcc                                                                                                                       >  2:893769/1‑148 (MQ=255)
                      gACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCa                                                                                                                      <  1:1078272/148‑1 (MQ=255)
                      gACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCa                                                                                                                      <  2:698557/148‑1 (MQ=255)
                        ccAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAac                                                                                                                    >  2:2565476/1‑148 (MQ=255)
                          aGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAacac                                                                                                                  <  2:1960849/148‑1 (MQ=255)
                                 cGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAgg                                                                                                           <  1:2008004/148‑1 (MQ=255)
                                    agaACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAg                                                                                                        >  1:482855/1‑148 (MQ=255)
                                      aaCGGAATCGACCAGCTCTCGTACCGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGtt                                                                                                      >  1:1901538/1‑148 (MQ=255)
                                       aCGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGttt                                                                                                     >  1:3178250/1‑148 (MQ=255)
                                        cGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTg                                                                                                    <  2:2894772/148‑1 (MQ=255)
                                          gAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCt                                                                                                  >  1:510939/1‑148 (MQ=255)
                                          gAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCt                                                                                                  >  1:1325130/1‑148 (MQ=255)
                                          gAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCt                                                                                                  <  2:2330208/148‑1 (MQ=255)
                                           aaTCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTc                                                                                                 >  1:2131254/1‑148 (MQ=255)
                                           aaTCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTc                                                                                                 >  1:822641/1‑148 (MQ=255)
                                             tCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGc                                                                                               >  2:2066306/1‑148 (MQ=255)
                                             tCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGc                                                                                               <  1:2953313/148‑1 (MQ=255)
                                              cGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGcc                                                                                              >  2:3024628/1‑148 (MQ=255)
                                               gACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCa                                                                                             <  1:1569747/148‑1 (MQ=255)
                                                aCCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAg                                                                                            >  2:1216231/1‑148 (MQ=255)
                                                 ccAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgt                                                                                           <  1:1844200/148‑1 (MQ=255)
                                                 ccAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgt                                                                                           >  1:845088/1‑148 (MQ=255)
                                                 ccAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgt                                                                                           >  1:1407392/1‑148 (MQ=255)
                                                   aGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgttg                                                                                         <  1:2837113/148‑1 (MQ=255)
                                                    gCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgttgt                                                                                        >  1:2445016/1‑148 (MQ=255)
                                                     ctctCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgttgtt                                                                                       >  2:2193146/1‑148 (MQ=255)
                                                     ctctCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgttgtt                                                                                       >  1:1306186/1‑148 (MQ=255)
                                                       ctcGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTcc                                                                                     >  2:1936691/1‑148 (MQ=255)
                                                             cAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGc                                                                               >  2:1780373/1‑148 (MQ=255)
                                                                cgccgcCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTcc                                                                            <  1:585815/148‑1 (MQ=255)
                                                                     ccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGc                                                                       <  2:2063853/148‑1 (MQ=255)
                                                                       aGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCtt                                                                     >  2:2630741/1‑148 (MQ=255)
                                                                          cacaGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCAc                                                                  >  1:897886/1‑148 (MQ=255)
                                                                          cacaGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCAc                                                                  >  2:2174635/1‑148 (MQ=255)
                                                                          cacaGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCAc                                                                  >  2:510518/1‑148 (MQ=255)
                                                                          cacaGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCAc                                                                  >  2:724039/1‑148 (MQ=255)
                                                                          cacaGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCAc                                                                  <  1:2049093/148‑1 (MQ=255)
                                                                           acaGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCAcc                                                                 >  1:1355223/1‑148 (MQ=255)
                                                                            caGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCg                                                                >  1:2601900/1‑148 (MQ=255)
                                                                              ggAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGt                                                              <  2:3050289/148‑1 (MQ=255)
                                                                              ggAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGt                                                              >  2:3208926/1‑148 (MQ=255)
                                                                               gAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTa                                                             >  1:1031507/1‑148 (MQ=255)
                                                                                 acacGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACt                                                           >  2:2090122/1‑148 (MQ=255)
                                                                                   acGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACttt                                                         <  1:66093/148‑1 (MQ=255)
                                                                                     gACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTa                                                       >  2:1760859/1‑148 (MQ=255)
                                                                                      aCAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTAc                                                      <  1:67929/148‑1 (MQ=255)
                                                                                       cAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTAcc                                                     <  1:1205565/148‑1 (MQ=255)
                                                                                        aaTCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTAccc                                                    >  1:2350253/1‑148 (MQ=255)
                                                                                        aaTCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTAccc                                                    <  2:2135449/148‑1 (MQ=255)
                                                                                           cAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGt                                                 >  2:1547049/1‑148 (MQ=255)
                                                                                            aaCGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTg                                                >  1:157404/1‑148 (MQ=255)
                                                                                             aCGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTgc                                               >  2:3222388/1‑148 (MQ=255)
                                                                                              cGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTgcc                                              >  2:2189827/1‑148 (MQ=255)
                                                                                                aaaTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTgccgc                                            <  1:938450/148‑1 (MQ=255)
                                                                                                aaaGCGCGTACAGTGCAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCAGAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTGACCCGGTgccgc                                            <  2:2047922/148‑1 (MQ=255)
                                                                                                  aTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCt                                          <  1:1768334/148‑1 (MQ=255)
                                                                                                  aTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCt                                          <  1:95342/148‑1 (MQ=255)
                                                                                                  aTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCt                                          >  2:2669155/1‑148 (MQ=255)
                                                                                                  aTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCt                                          >  1:2222697/1‑148 (MQ=255)
                                                                                                        tACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTa                                    <  1:893769/148‑1 (MQ=255)
                                                                                                          cAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAg                                  >  2:3032420/1‑148 (MQ=255)
                                                                                                          cAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAg                                  <  2:1306186/148‑1 (MQ=255)
                                                                                                           aGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGa                                 >  2:627231/1‑148 (MQ=255)
                                                                                                           aGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGATCCATTGCTTCACCGGTACTTTTACCCCGTGCCGCCTGGACTAAGa                                 >  2:2288908/1‑148 (MQ=255)
                                                                                                             tggaGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGAtt                               <  2:1619249/145‑1 (MQ=255)
                                                                                                             tGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGAtt                               >  1:164467/1‑148 (MQ=255)
                                                                                                               aaGGTTCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTc                             >  2:2431116/1‑148 (MQ=255)
                                                                                                               aaGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTc                             >  2:2681566/1‑148 (MQ=255)
                                                                                                                  gTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTAATAGCCAACACCGGTATGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCat                          >  1:2504794/1‑148 (MQ=255)
                                                                                                                    gCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCatgg                        >  2:2173888/1‑148 (MQ=255)
                                                                                                                     ccTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCatgga                       <  2:452244/148‑1 (MQ=255)
                                                                                                                        ggTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCatggatgg                    <  2:585023/148‑1 (MQ=255)
                                                                                                                            gCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAgg                <  1:3183608/148‑1 (MQ=255)
                                                                                                                            gCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAgg                >  2:1484754/1‑148 (MQ=255)
                                                                                                                            gCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAgg                >  2:1484753/1‑148 (MQ=255)
                                                                                                                                gagagaCGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGt            >  2:602284/1‑148 (MQ=255)
                                                                                                                                gagagaCGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGt            >  2:1178506/1‑148 (MQ=255)
                                                                                                                                 agagaCGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGtt           >  1:2073144/1‑148 (MQ=255)
                                                                                                                                  gagaCGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTg          <  1:3211961/148‑1 (MQ=255)
                                                                                                                                  gagaCGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTg          <  1:1381461/148‑1 (MQ=255)
                                                                                                                                   agaCGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGt         >  2:2249763/1‑148 (MQ=255)
                                                                                                                                     aCGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTaa       <  1:1487005/148‑1 (MQ=255)
                                                                                                                                        ttCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGt    >  1:474421/1‑148 (MQ=255)
                                                                                                                                             |                                                                                                                                                
CACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTC  >  CP009273/477126‑477411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑