Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 495,020 T→A 92.4% E137V (GAA→GTA)  aes ← acetyl esterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273495,0200TA92.4% 554.5 / 30.0 183E137V (GAA→GTA) aesacetyl esterase
Reads supporting (aligned to +/- strand):  ref base T (3/11);  new base A (89/80);  total (92/91)
Fisher's exact test for biased strand distribution p-value = 2.81e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

Only 100 of 184 total aligned reads displayed.

GCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAATAAA  >  CP009273/494875‑495167
                                                                                                                                                 |                                                                                                                                                   
gCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTAcc                                                                                                                                                   >  2:1982423/1‑148 (MQ=255)
  aaTCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACctc                                                                                                                                                 <  1:2429019/148‑1 (MQ=255)
        tCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGc                                                                                                                                           >  1:2010549/1‑148 (MQ=255)
         cTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCt                                                                                                                                          >  1:2492863/1‑148 (MQ=255)
          tGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCtt                                                                                                                                         >  2:2856061/1‑148 (MQ=255)
           gTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTg                                                                                                                                        <  2:542421/148‑1 (MQ=255)
           gTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTg                                                                                                                                        <  1:3134558/148‑1 (MQ=255)
            tttATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGc                                                                                                                                       >  2:69293/1‑148 (MQ=255)
            tttATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGc                                                                                                                                       >  2:1139800/1‑148 (MQ=255)
             ttATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCg                                                                                                                                      >  1:798971/1‑148 (MQ=255)
                tCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGaa                                                                                                                                   >  2:218268/1‑148 (MQ=255)
                 cACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGaaa                                                                                                                                  >  2:2481946/1‑148 (MQ=255)
                    gCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAAcg                                                                                                                               <  1:1705800/148‑1 (MQ=255)
                       aCCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACgcgc                                                                                                                            <  1:1676601/148‑1 (MQ=255)
                        ccACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCt                                                                                                                           >  2:2431942/1‑148 (MQ=255)
                        ccACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCt                                                                                                                           >  2:313610/1‑148 (MQ=255)
                         cacaACTCACTGGCGAGCGCCAGCATGTCAACTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTTGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCtt                                                                                                                          <  1:2187370/148‑1 (MQ=255)
                         cacaACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCtt                                                                                                                          <  2:1783974/148‑1 (MQ=255)
                          acaACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTc                                                                                                                         >  2:2547777/1‑148 (MQ=255)
                          acaACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTc                                                                                                                         <  2:1701255/148‑1 (MQ=255)
                            aaCGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAg                                                                                                                       >  2:2786978/1‑148 (MQ=255)
                                 aCTGGCGAGCTCCAGCATGGCACCTGCGTAATCACCTTCAAAGCAAATGCTGGACATATTGATTTGATAATCCTACGACTGCTGGTTGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGTTGaa                                                                                                                  <  2:782925/148‑1 (MQ=255)
                                 aCTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGaa                                                                                                                  <  2:1365973/148‑1 (MQ=255)
                                          cgcCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTaa                                                                                                         >  1:654956/1‑148 (MQ=255)
                                          cgcCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTaa                                                                                                         >  1:491075/1‑148 (MQ=255)
                                          cgcCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTaa                                                                                                         >  2:1583739/1‑148 (MQ=255)
                                             cAGCATGGCACCTGCGGAATCTCCGGCAAAGCCAATGCGGGACTTATTGATATGATAATCCTCCGCATGCTTGTGGAAAGAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCa                                                                                                      <  2:1963918/148‑1 (MQ=255)
                                             cAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCa                                                                                                      <  2:1963135/148‑1 (MQ=255)
                                             cAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCa                                                                                                      >  2:2364288/1‑148 (MQ=255)
                                                cATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATa                                                                                                   >  1:1239548/1‑148 (MQ=255)
                                                   ggCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCa                                                                                                <  1:584774/148‑1 (MQ=255)
                                                   ggCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCa                                                                                                <  1:986329/148‑1 (MQ=255)
                                                          gCGGAATCACCGGCAAAGCCTATGCTGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCg                                                                                         <  1:1780585/148‑1 (MQ=255)
                                                               aTCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACAt                                                                                    <  2:3123803/148‑1 (MQ=255)
                                                               aTCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACAt                                                                                    >  2:2170295/1‑148 (MQ=255)
                                                               aTCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACAt                                                                                    >  2:509104/1‑148 (MQ=255)
                                                                tCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACAtt                                                                                   >  2:441770/1‑148 (MQ=255)
                                                                tCACCGACAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACAtt                                                                                   <  2:1351829/148‑1 (MQ=255)
                                                                    cGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCt                                                                               <  1:2099858/148‑1 (MQ=255)
                                                                      gCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGt                                                                             >  1:2314938/1‑148 (MQ=255)
                                                                       cAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTa                                                                            <  1:2364288/148‑1 (MQ=255)
                                                                        aaaGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAg                                                                           >  1:3298183/1‑148 (MQ=255)
                                                                         aaGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGc                                                                          <  2:31732/148‑1 (MQ=255)
                                                                         aaGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGc                                                                          >  2:2956017/1‑148 (MQ=255)
                                                                              aaTGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCATGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGcc                                                                     <  1:616279/148‑1 (MQ=255)
                                                                              aaTGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGcc                                                                     >  1:2594391/1‑148 (MQ=255)
                                                                              aaTGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGcc                                                                     >  1:1501414/1‑148 (MQ=255)
                                                                               aTGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCca                                                                    >  2:1009355/1‑148 (MQ=255)
                                                                                tGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCcag                                                                   >  2:2478885/1‑148 (MQ=255)
                                                                                   gggACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCcagcag                                                                <  1:2937429/148‑1 (MQ=255)
                                                                                       cATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGgcgc                                                            <  1:605234/148‑1 (MQ=255)
                                                                                         tatTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCat                                                          >  2:3168794/1‑148 (MQ=255)
                                                                                          atTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTTCATTGGCTGTAGCTTGCCAGCAGGCGCatg                                                         <  1:555082/148‑1 (MQ=255)
                                                                                          atTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTTCCAGCAGGCGCatg                                                         >  1:1511533/1‑148 (MQ=255)
                                                                                           ttgatttgatAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCatga                                                        >  1:2792360/1‑148 (MQ=255)
                                                                                           ttgatttgatAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCatga                                                        >  2:1881611/1‑148 (MQ=255)
                                                                                           ttgatttgatAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCatga                                                        >  2:1881704/1‑148 (MQ=255)
                                                                                               tttgatAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCg                                                    >  1:2936335/1‑148 (MQ=255)
                                                                                               tttgatAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCg                                                    >  1:1331841/1‑148 (MQ=255)
                                                                                                ttgatAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGa                                                   <  1:808730/148‑1 (MQ=255)
                                                                                                ttgatAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGa                                                   >  2:404524/1‑148 (MQ=255)
                                                                                                   atAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCg                                                >  2:699076/1‑148 (MQ=255)
                                                                                                   atAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCg                                                <  1:1542035/148‑1 (MQ=255)
                                                                                                    tAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGt                                               >  2:2099829/1‑148 (MQ=255)
                                                                                                       tcctccGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTggg                                            >  1:3180729/1‑148 (MQ=255)
                                                                                                        cctccGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGt                                           >  2:769168/1‑148 (MQ=255)
                                                                                                        cctccGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGt                                           <  1:1008882/148‑1 (MQ=255)
                                                                                                        cctccGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGt                                           >  2:369341/1‑148 (MQ=255)
                                                                                                        cctccGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGt                                           <  1:1007709/148‑1 (MQ=255)
                                                                                                        cctccGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGt                                           <  1:1097043/148‑1 (MQ=255)
                                                                                                            cGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCg                                       <  1:878624/148‑1 (MQ=255)
                                                                                                              ccTGCTGGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCgag                                     <  2:799453/148‑1 (MQ=255)
                                                                                                               ctgctgGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCgaga                                    <  1:1613176/148‑1 (MQ=255)
                                                                                                                tgctgGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGAt                                   <  1:69293/148‑1 (MQ=255)
                                                                                                                 gctgGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGAtt                                  >  1:773180/1‑148 (MQ=255)
                                                                                                                 gctgGTGGAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGAtt                                  >  2:2754338/1‑148 (MQ=255)
                                                                                                                   tggtggAAATAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGc                                <  2:2375270/148‑1 (MQ=255)
                                                                                                                         aaaaaaCAACAAGCAGCCACAAATACCTCTATCGCTTGCGCAAAACGCCCTTCAGGTGAAAGGGAGTAATCAAAAACAAACACCGTAAATAGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGAATGCCGAGaa                          >  2:2931233/1‑148 (MQ=255)
                                                                                                                         aaaTAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGaa                          >  2:1527101/1‑148 (MQ=255)
                                                                                                                         aaaTAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGaa                          <  2:461020/148‑1 (MQ=255)
                                                                                                                         aaaTAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGaa                          >  2:2504137/1‑148 (MQ=255)
                                                                                                                           aTAACAACAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATa                        >  1:292172/1‑148 (MQ=255)
                                                                                                                             aacaacAAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATaaa                      <  1:1690438/148‑1 (MQ=255)
                                                                                                                              acaacaAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATaaaa                     <  2:23360/148‑1 (MQ=255)
                                                                                                                              acaacaAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATaaaa                     <  1:2170295/148‑1 (MQ=255)
                                                                                                                              acaacaAGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATaaaa                     >  1:2096501/1‑148 (MQ=255)
                                                                                                                               caacaaGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAAc                    <  1:502628/148‑1 (MQ=255)
                                                                                                                               caacaaGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAAc                    <  1:3121172/148‑1 (MQ=255)
                                                                                                                               caacaaGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAAc                    <  1:1426263/148‑1 (MQ=255)
                                                                                                                                  caaGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGc                 >  1:2043891/1‑148 (MQ=255)
                                                                                                                                   aaGCAGCCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGcc                <  1:248765/148‑1 (MQ=255)
                                                                                                                                     gcagcCACAATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTc              >  1:969332/1‑148 (MQ=255)
                                                                                                                                     gcagcCAAAATGACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGAATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTc              <  2:2266925/148‑1 (MQ=255)
                                                                                                                                            caATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCaa       <  2:2594391/148‑1 (MQ=255)
                                                                                                                                            caATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCaa       <  2:1501405/148‑1 (MQ=255)
                                                                                                                                            caATTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCaa       <  2:1501440/148‑1 (MQ=255)
                                                                                                                                            caATTACCTCTATAGCTTGAGGAAAACGCTGTTCAGGTGAAGGGTTGTAATCAATACCAATCACTGTACATTGGCTGTAGCTTTCCAGCAGGGGCTTGTTGGGTTATTGGTTATCGATATTTCCGTTAATAAAACTGCATCATTGCaa       <  2:1857756/148‑1 (MQ=255)
                                                                                                                                              aTTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAATGGGTGTAATCAATACCAATCCCCGTACATGGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTTTCGAGATTGCCGAGAATAAAACCGCCTCCATGCaaat     <  1:1485643/148‑1 (MQ=255)
                                                                                                                                              aTTACCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGTTGCGATCGTGGGTATCGAGATTGCCGATAATAAAACCGCCTCCATGCaaat     <  1:1487530/148‑1 (MQ=255)
                                                                                                                                                 tCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCTTACATTGGCTGTAGCTTGCCAGCCGGCGCATGATGCGATGGTGGGTATCGAGATTGCCGAGAATAAACCCGCCTCCATGCaaataaa  <  1:2786978/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAATCAATACCAATCACCGTACATTGGCTGTAGCTTGCCAGCAGGCGCATGATGCGATCGTGGGTATCGAGATTGCCGAGAATAAAACCGCCTCCATGCAAATAAA  >  CP009273/494875‑495167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑