Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 524,496 T→G 91.2% pseudogene (401/491 nt) ybbD → pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273524,4960TG91.2% 716.0 / 28.9 204pseudogene (401/491 nt)ybbDpseudogene
Reads supporting (aligned to +/- strand):  ref base T (9/9);  new base G (81/105);  total (90/114)
Fisher's exact test for biased strand distribution p-value = 6.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 205 total aligned reads displayed.

TCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGG  >  CP009273/524350‑524639
                                                                                                                                                  |                                                                                                                                               
tCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGgtg                                                                                                                                                <  1:3305739/148‑1 (MQ=255)
  ccTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTa                                                                                                                                              >  2:1188538/1‑148 (MQ=255)
   cTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTaa                                                                                                                                             <  1:3225575/148‑1 (MQ=255)
   cTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTaa                                                                                                                                             <  2:2957835/148‑1 (MQ=255)
    tAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTaaa                                                                                                                                            <  2:1771499/148‑1 (MQ=255)
     aaCAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAAc                                                                                                                                           >  2:2343979/1‑148 (MQ=255)
      aCAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAAcc                                                                                                                                          >  2:346093/1‑148 (MQ=255)
      aCAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAAcc                                                                                                                                          >  1:720064/1‑148 (MQ=255)
         aaTGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCttt                                                                                                                                       <  2:711277/148‑1 (MQ=255)
         aaTGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCttt                                                                                                                                       <  1:1596110/148‑1 (MQ=255)
          atgatgatgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCtttt                                                                                                                                      <  2:481885/148‑1 (MQ=255)
          atgatgatgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCtttt                                                                                                                                      <  1:1264956/148‑1 (MQ=255)
            gatgatgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCtttttt                                                                                                                                    <  2:1108603/148‑1 (MQ=255)
            gatgatgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCtttttt                                                                                                                                    <  1:2822138/148‑1 (MQ=255)
            gatgatgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCtttttt                                                                                                                                    <  1:1125409/148‑1 (MQ=255)
              tgatgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCtttttttt                                                                                                                                  <  2:380854/148‑1 (MQ=255)
               gatgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTttg                                                                                                                                 >  1:1047239/1‑148 (MQ=255)
                atgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTttgt                                                                                                                                <  1:2759955/148‑1 (MQ=255)
                atgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTttgt                                                                                                                                <  1:2109548/148‑1 (MQ=255)
                atgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTttgt                                                                                                                                <  2:2242183/148‑1 (MQ=255)
                   ggggTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTgg                                                                                                                             <  1:107533/148‑1 (MQ=255)
                   ggggTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTgg                                                                                                                             <  1:2269098/148‑1 (MQ=255)
                    gggTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCTTTTTTTTGTTGGt                                                                                                                            >  1:2556552/1‑148 (MQ=255)
                       tAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGaa                                                                                                                         <  1:503775/148‑1 (MQ=255)
                       tAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGaa                                                                                                                         <  1:503756/148‑1 (MQ=255)
                        aGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGaaa                                                                                                                        <  1:1912354/148‑1 (MQ=255)
                              aaGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATg                                                                                                                  <  2:595092/148‑1 (MQ=255)
                              aaGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATg                                                                                                                  <  2:1704305/148‑1 (MQ=255)
                               aGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGTGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGt                                                                                                                 <  1:651386/148‑1 (MQ=255)
                                gAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCGAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCGGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGtt                                                                                                                >  2:1454139/1‑148 (MQ=255)
                                gAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGtt                                                                                                                <  1:1716238/148‑1 (MQ=255)
                                gAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGtt                                                                                                                <  1:1310829/148‑1 (MQ=255)
                                gAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGtt                                                                                                                >  1:2394388/1‑148 (MQ=255)
                                 aaaaCATTTACCGTGAGGCCAAGGTAACAAGTGGCGAAAATCTAAACAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAATTTACCTGGGAACGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTa                                                                                                               <  2:2769025/148‑1 (MQ=255)
                                   aaCATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACg                                                                                                             >  1:331205/1‑148 (MQ=255)
                                    aCATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGc                                                                                                            <  2:2443015/148‑1 (MQ=255)
                                       ttGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCatt                                                                                                         >  1:2871075/1‑148 (MQ=255)
                                       ttGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCatt                                                                                                         <  2:1977749/148‑1 (MQ=255)
                                         gACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCattat                                                                                                       >  1:2491143/1‑148 (MQ=255)
                                           cAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTAttt                                                                                                     >  2:2761030/1‑148 (MQ=255)
                                              tGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTtat                                                                                                  <  2:2333663/148‑1 (MQ=255)
                                               gAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTtata                                                                                                 >  2:88189/1‑148 (MQ=255)
                                                     cAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGCAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAat                                                                                           <  1:928426/148‑1 (MQ=255)
                                                        ggAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATAtt                                                                                        <  2:798830/148‑1 (MQ=255)
                                                        ggAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATAtt                                                                                        >  1:977121/1‑148 (MQ=255)
                                                         gAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTg                                                                                       <  2:3250167/148‑1 (MQ=255)
                                                          aaCAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTgg                                                                                      <  1:1087831/148‑1 (MQ=255)
                                                               gTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTg                                                                                 <  1:697513/148‑1 (MQ=255)
                                                                   cGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGAtt                                                                             >  2:2649223/1‑148 (MQ=255)
                                                                        aTCGTATCAAAGAATGATCCAGATACAAAACAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGCGATGGTGTAAACCTTTATTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGAtagactc                                                                        >  2:3126239/1‑142 (MQ=255)
                                                                        aTCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTg                                                                        >  2:2411039/1‑148 (MQ=255)
                                                                         tCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGa                                                                       <  1:2950935/148‑1 (MQ=255)
                                                                          cGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGAt                                                                      <  2:409421/148‑1 (MQ=255)
                                                                           gTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTT‑TTGGTGAAATTAATTTTACGCATTATTTTATAACAAATATTGGAGCTGGATTTCCTGATGc                                                                    >  2:2718890/1‑148 (MQ=255)
                                                                           gTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGc                                                                    >  2:160840/1‑148 (MQ=255)
                                                                            tATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGc                                                                    <  1:1148164/148‑1 (MQ=255)
                                                                               cAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCCCCGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTtg                                                                 <  2:1945872/148‑1 (MQ=255)
                                                                                 aaGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTtgtg                                                               >  2:671546/1‑148 (MQ=255)
                                                                                 aaGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTtgtg                                                               >  2:2917030/1‑148 (MQ=255)
                                                                                  aGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGc                                                              <  1:1303566/148‑1 (MQ=255)
                                                                                  aGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGc                                                              >  1:894908/1‑148 (MQ=255)
                                                                                  aGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGc                                                              >  2:2034920/1‑148 (MQ=255)
                                                                                    aaTGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCag                                                            >  1:846790/1‑148 (MQ=255)
                                                                                       gATCCAGATACAAAAAAAGTGTTGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGt                                                         >  2:1352030/1‑148 (MQ=255)
                                                                                        aTCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTa                                                        <  1:2263927/148‑1 (MQ=255)
                                                                                         tCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTaa                                                       <  1:2590164/148‑1 (MQ=255)
                                                                                          ccAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAAt                                                      <  2:162205/148‑1 (MQ=255)
                                                                                          ccAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAAt                                                      <  1:2741528/148‑1 (MQ=255)
                                                                                            aGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTg                                                    <  1:1538795/148‑1 (MQ=255)
                                                                                             gATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGc                                                   >  1:1330543/1‑148 (MQ=255)
                                                                                               tACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCtt                                                 <  1:1364480/148‑1 (MQ=255)
                                                                                                  aaaaaaaGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGaa                                              <  1:2048015/148‑1 (MQ=255)
                                                                                                  aaaaaaaGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGaa                                              >  1:3182846/1‑148 (MQ=255)
                                                                                                   aaaaaaGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTTTTTGTTGAATTAAAGTTAAGCATTATTTTATAACAAATATTGGAGCTGGTTTGCCTGATGCTTGTTCAGAGTAATTGCGTGAAt                                             >  1:879094/1‑148 (MQ=255)
                                                                                                   aaaaaaGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAAt                                             >  2:540565/1‑148 (MQ=255)
                                                                                                    aaaaaGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAAtt                                            >  1:2002934/1‑148 (MQ=255)
                                                                                                    aaaaaGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAAtt                                            >  2:721921/1‑148 (MQ=255)
                                                                                                      aaaGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTaa                                          >  1:1365810/1‑148 (MQ=255)
                                                                                                      aaaGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTaa                                          >  2:719393/1‑148 (MQ=255)
                                                                                                       aaGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAag                                         <  2:2547063/148‑1 (MQ=255)
                                                                                                       aaGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAag                                         <  2:3076305/148‑1 (MQ=255)
                                                                                                         gtgtGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAagag                                       <  1:2343979/148‑1 (MQ=255)
                                                                                                          tgtgGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGt                                      >  2:1736590/1‑148 (MQ=255)
                                                                                                          tgtgGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGGTGGTTAAAATAAATTTAAGCCATATTTTATAAAAAAAAATGGAGGTTGATTTGCGTATTCTTTTTCCGTTTAATTTCGTGAATTAagatt                                      >  2:63012/1‑146 (MQ=255)
                                                                                                            tggtgTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCt                                    >  2:3094557/1‑148 (MQ=255)
                                                                                                                gtAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATcc                                >  1:606083/1‑148 (MQ=255)
                                                                                                                 tAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATccc                               <  1:2602192/148‑1 (MQ=255)
                                                                                                                    atatGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCata                            >  2:564718/1‑148 (MQ=255)
                                                                                                                       tGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCg                         >  2:2572800/1‑148 (MQ=255)
                                                                                                                           aaGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAgt                     >  1:1299796/1‑148 (MQ=255)
                                                                                                                            aGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAgtc                    >  1:366243/1‑148 (MQ=255)
                                                                                                                            aGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAgtc                    >  1:3098304/1‑148 (MQ=255)
                                                                                                                            aGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAgtc                    <  2:2071804/148‑1 (MQ=255)
                                                                                                                                  cAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAAc              >  2:1566265/1‑148 (MQ=255)
                                                                                                                                      gCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCg          <  1:758699/148‑1 (MQ=255)
                                                                                                                                       cAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGt         >  2:1617084/1‑148 (MQ=255)
                                                                                                                                           gggATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTg     <  1:1496681/148‑1 (MQ=255)
                                                                                                                                            ggATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGa    <  1:993434/148‑1 (MQ=255)
                                                                                                                                            ggATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGa    <  1:2392928/148‑1 (MQ=255)
                                                                                                                                              aTGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAgg  <  2:1365810/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
TCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGG  >  CP009273/524350‑524639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑