Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 557,303 A→T 11.0% E717D (GAA→GAT)  sfmD → putative outer membrane export usher protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273557,3030AT11.0% 637.3 / 57.3 215E717D (GAA→GAT) sfmDputative outer membrane export usher protein
Reads supporting (aligned to +/- strand):  ref base A (107/84);  new base T (13/11);  total (120/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 219 total aligned reads displayed.

TATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAATATTAGCAGCGTTGTGCCGAC  >  CP009273/557157‑557449
                                                                                                                                                  |                                                                                                                                                  
tataACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGTCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGaaa                                                                                                                                                   <  2:2698794/148‑1 (MQ=255)
 ataACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGaaaa                                                                                                                                                  >  2:3119017/1‑148 (MQ=255)
 agaaCTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTTaaaa                                                                                                                                                  <  2:2073089/146‑1 (MQ=255)
   aaCTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATc                                                                                                                                                <  2:847249/148‑1 (MQ=255)
   aaCTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATc                                                                                                                                                >  2:2528523/1‑148 (MQ=255)
     cTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCaa                                                                                                                                              >  1:598418/1‑148 (MQ=255)
      tACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGATAATCaaa                                                                                                                                             >  2:1808354/1‑148 (MQ=255)
      tACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGATAATCaaa                                                                                                                                             >  2:136260/1‑148 (MQ=255)
      tACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCaaa                                                                                                                                             >  2:1210027/1‑148 (MQ=255)
      tACGACCACGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCaaa                                                                                                                                             >  1:257791/1‑148 (MQ=255)
       aCGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAAc                                                                                                                                            <  2:2283418/148‑1 (MQ=255)
          aCCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTgg                                                                                                                                         >  1:2719214/1‑148 (MQ=255)
           ccGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGc                                                                                                                                        >  1:1439731/1‑148 (MQ=255)
               gATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCAttt                                                                                                                                    <  2:1340166/148‑1 (MQ=255)
               gATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCAttt                                                                                                                                    <  1:1866303/148‑1 (MQ=255)
                 tCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTa                                                                                                                                  >  1:1648032/1‑148 (MQ=255)
                     cATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCg                                                                                                                              <  1:3313280/148‑1 (MQ=255)
                     cATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCg                                                                                                                              >  2:1800036/1‑148 (MQ=255)
                        gACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACt                                                                                                                           >  2:637144/1‑148 (MQ=255)
                             tAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGgcgc                                                                                                                      >  1:572919/1‑148 (MQ=255)
                               aCTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCgg                                                                                                                    <  2:383162/148‑1 (MQ=255)
                                  ggCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGATAATCAAACTGGCATTTTAACCGACTGGCGCGGCTa                                                                                                                 <  2:36599/148‑1 (MQ=255)
                                      gCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGATAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCg                                                                                                             >  2:2465990/1‑148 (MQ=255)
                                       cTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCgg                                                                                                            >  2:2552093/1‑148 (MQ=255)
                                       cTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCgg                                                                                                            >  2:1027887/1‑148 (MQ=255)
                                         gTCTGGCGGGGGGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGAGACCAATGTTTTGATTACAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTCACCGCCTGGCGCGGCTATGCGGTg                                                                                                          <  2:1731142/148‑1 (MQ=255)
                                          tCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGa                                                                                                         >  2:2168584/1‑148 (MQ=255)
                                           cTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGAt                                                                                                        >  2:1229330/1‑148 (MQ=255)
                                              gCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCt                                                                                                     >  2:3111443/1‑148 (MQ=255)
                                                ggTTTGGTGGTGCATGTACATGGCATATCGCTGTGCCAGCCTTTAGGGGATTCCAATGTTTGGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCTTTTTAACCGAGTGGCGCGGCTTTGCGGTGATGCTGt                                                                                                   <  2:2406437/148‑1 (MQ=255)
                                                    tgGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGATAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGc                                                                                               >  1:1652435/1‑148 (MQ=255)
                                                         gggCATGAAAATGGCATACCGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACgg                                                                                          <  1:1688118/148‑1 (MQ=255)
                                                             aTGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTa                                                                                      <  1:2748082/148‑1 (MQ=255)
                                                              tGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTAt                                                                                     <  1:1058963/148‑1 (MQ=255)
                                                               gAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGATAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATc                                                                                    <  1:861503/148‑1 (MQ=255)
                                                                aaaaTGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCg                                                                                   <  1:3178230/148‑1 (MQ=255)
                                                                aaaaTGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCg                                                                                   >  2:1859944/1‑148 (MQ=255)
                                                                aaaaTGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCg                                                                                   >  1:2471844/1‑148 (MQ=255)
                                                                 aaaTGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCgg                                                                                  >  2:2667086/1‑148 (MQ=255)
                                                                   aTGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGta                                                                                <  2:257088/148‑1 (MQ=255)
                                                                      gCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATaa                                                                             >  2:3114673/1‑148 (MQ=255)
                                                                              cTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATcg                                                                     <  2:1974634/148‑1 (MQ=255)
                                                                              cTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATcg                                                                     <  1:316068/148‑1 (MQ=255)
                                                                                gAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATcgcg                                                                   >  2:851243/1‑148 (MQ=255)
                                                                                   ccagccTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCtt                                                                >  1:297719/1‑148 (MQ=255)
                                                                                   ccagccTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCtt                                                                >  1:296000/1‑148 (MQ=255)
                                                                                   ccagccTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCtt                                                                <  1:104217/148‑1 (MQ=255)
                                                                                      gccTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGAt                                                             <  2:278548/148‑1 (MQ=255)
                                                                                       ccTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATa                                                            >  1:163147/1‑148 (MQ=255)
                                                                                       ccTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATa                                                            >  1:163538/1‑148 (MQ=255)
                                                                                         tttAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATAcc                                                          >  2:111134/1‑148 (MQ=255)
                                                                                          ttAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGATAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCa                                                         >  2:7035/1‑148 (MQ=255)
                                                                                          ttAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGATAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCa                                                         >  2:3085846/1‑148 (MQ=255)
                                                                                          ttAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCa                                                         <  1:1789822/148‑1 (MQ=255)
                                                                                              gggATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATAc                                                     >  2:2696754/1‑148 (MQ=255)
                                                                                               ggATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACg                                                    >  1:2908879/1‑148 (MQ=255)
                                                                                                 aTACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGAt                                                  <  1:2715511/148‑1 (MQ=255)
                                                                                                   aCCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATgg                                                >  2:944410/1‑148 (MQ=255)
                                                                                                    ccAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATggg                                               >  1:2194925/1‑148 (MQ=255)
                                                                                                        tGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAAt                                           >  2:780392/1‑148 (MQ=255)
                                                                                                         gTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAAtt                                          <  2:2479969/148‑1 (MQ=255)
                                                                                                         gTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAAtt                                          >  2:2019236/1‑148 (MQ=255)
                                                                                                           tttGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGATAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTcc                                        >  2:672017/1‑148 (MQ=255)
                                                                                                            ttGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCa                                       <  1:3114650/148‑1 (MQ=255)
                                                                                                            ttGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCa                                       <  2:2643454/148‑1 (MQ=255)
                                                                                                                ttAAAGCGCCTGGCGCAGGCGGTGTACGCATTGATAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGa                                   >  2:2864049/1‑148 (MQ=255)
                                                                                                                ttAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGa                                   >  1:1591263/1‑148 (MQ=255)
                                                                                                                    aGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGtt                               <  2:981453/148‑1 (MQ=255)
                                                                                                                    aGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGtt                               <  2:1264282/148‑1 (MQ=255)
                                                                                                                        ccTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGaaa                           >  2:1820827/1‑148 (MQ=255)
                                                                                                                           ggCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGaaaaaa                        >  2:3175732/1‑148 (MQ=255)
                                                                                                                            gcgcAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGaaaaaaa                       >  2:2132827/1‑148 (MQ=255)
                                                                                                                            gcgcAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGaaaaaaa                       <  1:1274638/148‑1 (MQ=255)
                                                                                                                            gcgcAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGaaaaaaa                       >  2:1984945/1‑148 (MQ=255)
                                                                                                                             cgcAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAat                      <  2:247400/148‑1 (MQ=255)
                                                                                                                             cgcAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAat                      <  2:30227/148‑1 (MQ=255)
                                                                                                                              gcAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAata                     <  1:865275/148‑1 (MQ=255)
                                                                                                                               cAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAatat                    >  1:2141866/1‑148 (MQ=255)
                                                                                                                               cAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAatat                    <  1:3119017/148‑1 (MQ=255)
                                                                                                                                aGGCGGTGTACGCATTGATAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAATAtt                   <  1:136260/148‑1 (MQ=255)
                                                                                                                                aGGCGGTGTACGCATTGATAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAATAtt                   <  1:1808354/148‑1 (MQ=255)
                                                                                                                                 ggCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAATATTa                  >  1:2203632/1‑148 (MQ=255)
                                                                                                                                  gCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAATATTag                 <  1:1386916/148‑1 (MQ=255)
                                                                                                                                   cGGTGTACGCATTGATAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAATATTagc                <  2:2356184/148‑1 (MQ=255)
                                                                                                                                    ggTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAATATTagca               <  2:1648032/148‑1 (MQ=255)
                                                                                                                                      tgtACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAATATTagcagc             <  1:2168576/148‑1 (MQ=255)
                                                                                                                                       gtACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAATATTAGCAGCg            <  2:1439731/148‑1 (MQ=255)
                                                                                                                                        tACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAATATTAGCAGCGt           >  2:1700566/1‑148 (MQ=255)
                                                                                                                                        tACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAATATTAGCAGCGt           >  2:328067/1‑148 (MQ=255)
                                                                                                                                        tACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAATATTAGCAGCGt           >  2:584365/1‑148 (MQ=255)
                                                                                                                                        tACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAATATTAGCAGCGt           >  2:584448/1‑148 (MQ=255)
                                                                                                                                         aCGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAATATTAGCAGCGtt          >  1:2529758/1‑148 (MQ=255)
                                                                                                                                            cATTGATAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAATATTAGCAGCGTtgtg       <  1:3046221/148‑1 (MQ=255)
                                                                                                                                            cATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAATATTAGCAGCGTtgtg       <  1:2528523/148‑1 (MQ=255)
                                                                                                                                            cATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAATATTAGCAGCGTtgtg       >  1:493162/1‑148 (MQ=255)
                                                                                                                                             aTTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAATATTAGCAGCGTTGTGc      >  2:2537726/1‑148 (MQ=255)
                                                                                                                                              ttGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAATATTAGCAGCGTTGTGcc     >  1:2342309/1‑148 (MQ=255)
                                                                                                                                              ttGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAATATTAGCAGCGTTGTGcc     >  1:680439/1‑148 (MQ=255)
                                                                                                                                               tGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAATATTAGCAGCGTTGTGCCg    <  2:3060253/148‑1 (MQ=255)
                                                                                                                                                 aaaaTCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAATATTAGCAGCGTTGTGCCGAc  >  1:1097917/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAATATTAGCAGCGTTGTGCCGAC  >  CP009273/557157‑557449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑