Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 581,633 G→T 7.2% L244L (CTC→CTA)  envY ← porin thermoregulatory transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273581,6330GT 7.2% 818.0 / 24.0 221L244L (CTC→CTA) envYporin thermoregulatory transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (99/106);  new base T (5/11);  total (104/117)
Fisher's exact test for biased strand distribution p-value = 2.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 221 total aligned reads displayed.

GACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTC  >  CP009273/581490‑581779
                                                                                                                                               |                                                                                                                                                  
gACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGAt                                                                                                                                                >  2:2444175/1‑148 (MQ=255)
 aCTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATa                                                                                                                                               >  1:436379/1‑148 (MQ=255)
 aCTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATa                                                                                                                                               >  1:2101185/1‑148 (MQ=255)
  cTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATaa                                                                                                                                              <  1:2871569/148‑1 (MQ=255)
    tAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAAtt                                                                                                                                            <  1:2244046/148‑1 (MQ=255)
    tAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAAtt                                                                                                                                            <  2:3073554/148‑1 (MQ=255)
      aaaTGTCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCa                                                                                                                                          >  1:854395/1‑148 (MQ=255)
      aaaTGTCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCa                                                                                                                                          <  2:658176/148‑1 (MQ=255)
      aaaTGTCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCa                                                                                                                                          <  2:427429/148‑1 (MQ=255)
          gTCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACgg                                                                                                                                      >  2:1703596/1‑148 (MQ=255)
           tCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGgt                                                                                                                                     <  1:1318311/148‑1 (MQ=255)
           tCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGgt                                                                                                                                     <  1:1297499/148‑1 (MQ=255)
           tCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGgt                                                                                                                                     <  1:1318232/148‑1 (MQ=255)
            cGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGgtg                                                                                                                                    >  2:892971/1‑148 (MQ=255)
            cGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGgtg                                                                                                                                    <  1:343931/148‑1 (MQ=255)
                    gAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCg                                                                                                                            >  2:3007266/1‑148 (MQ=255)
                        ttCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTaaa                                                                                                                        >  2:1633232/1‑148 (MQ=255)
                        ttCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTaaa                                                                                                                        <  2:379426/148‑1 (MQ=255)
                        ttCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCGTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTaaa                                                                                                                        <  2:2189613/148‑1 (MQ=255)
                         tCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAAGGGTGTCAGGCCGTaaaa                                                                                                                       >  1:2146653/1‑148 (MQ=255)
                         tCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTaaaa                                                                                                                       <  1:580856/148‑1 (MQ=255)
                         tCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTaaaa                                                                                                                       >  2:741950/1‑148 (MQ=255)
                                    ggCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTaaaaa                                                                                                            >  2:247166/1‑148 (MQ=255)
                                       cGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACag                                                                                                         <  2:3225553/148‑1 (MQ=255)
                                        gCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACaga                                                                                                        <  1:2131638/148‑1 (MQ=255)
                                         cAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACagag                                                                                                       >  1:2636165/1‑148 (MQ=255)
                                            gTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATa                                                                                                    >  2:1001880/1‑148 (MQ=255)
                                            gTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATa                                                                                                    >  2:143270/1‑148 (MQ=255)
                                            gTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATa                                                                                                    >  2:2262/1‑148 (MQ=255)
                                             ttCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATaa                                                                                                   >  1:2559294/1‑148 (MQ=255)
                                             ttCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATaa                                                                                                   <  2:1165998/148‑1 (MQ=255)
                                               cGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAg                                                                                                 <  2:2859337/148‑1 (MQ=255)
                                                gAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGt                                                                                                <  2:1863234/148‑1 (MQ=255)
                                                gAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGt                                                                                                <  2:564544/148‑1 (MQ=255)
                                                      tGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTacgacg                                                                                          >  1:184262/1‑148 (MQ=255)
                                                       gCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGt                                                                                         >  1:1256203/1‑148 (MQ=255)
                                                        cAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGtg                                                                                        <  1:1384204/148‑1 (MQ=255)
                                                        cAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGtg                                                                                        <  2:333362/148‑1 (MQ=255)
                                                         aGGGCGCGCCATTTCGTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGtgc                                                                                       <  2:1403784/148‑1 (MQ=255)
                                                           ggCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGtgctg                                                                                     >  1:2801874/1‑148 (MQ=255)
                                                             cgcgcCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCACCGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTgctgct                                                                                   <  2:862586/148‑1 (MQ=255)
                                                             cgcgcCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTgctgct                                                                                   >  1:2784254/1‑148 (MQ=255)
                                                              gcgcCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCta                                                                                  <  2:2356063/148‑1 (MQ=255)
                                                              gcgcCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCta                                                                                  <  2:268569/148‑1 (MQ=255)
                                                                 ccATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAg                                                                               <  2:2092660/148‑1 (MQ=255)
                                                                   aTTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGcc                                                                             >  2:596253/1‑148 (MQ=255)
                                                                      tcttcCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCcaca                                                                          >  1:3271136/1‑148 (MQ=255)
                                                                       cttcCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACAt                                                                         >  2:2070257/1‑148 (MQ=255)
                                                                         tcCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATaa                                                                       >  2:2451151/1‑148 (MQ=255)
                                                                         tcCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATaa                                                                       <  1:166514/148‑1 (MQ=255)
                                                                         tcCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATaa                                                                       <  2:86464/148‑1 (MQ=255)
                                                                             cATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTgc                                                                   >  2:502778/1‑148 (MQ=255)
                                                                             cATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTgc                                                                   >  1:1696227/1‑148 (MQ=255)
                                                                             cATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTgc                                                                   >  1:1696719/1‑148 (MQ=255)
                                                                             cATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTgc                                                                   >  2:8661/1‑148 (MQ=255)
                                                                              aTTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTgcg                                                                  <  1:687541/148‑1 (MQ=255)
                                                                               tttATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGtg                                                                                        >  1:161357/1‑125 (MQ=255)
                                                                                ttATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGcc                                                                >  1:927547/1‑148 (MQ=255)
                                                                                ttATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGcc                                                                >  1:1092363/1‑148 (MQ=255)
                                                                                  aTGCCCGTCTTATCCGTTTCCGCTTTTCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCTACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCAc                                                              <  2:2756387/148‑1 (MQ=255)
                                                                                  aTGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCAc                                                              <  1:3167645/148‑1 (MQ=255)
                                                                                   tGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCAcc                                                             >  2:676042/1‑148 (MQ=255)
                                                                                   tGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCAcc                                                             <  1:1095415/148‑1 (MQ=255)
                                                                                     cccGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTg                                                           >  2:449013/1‑148 (MQ=255)
                                                                                      ccGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGa                                                          >  1:2755314/1‑148 (MQ=255)
                                                                                        gTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTGTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGt                                                        <  2:1291713/148‑1 (MQ=255)
                                                                                         tCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTg                                                       <  1:2444175/148‑1 (MQ=255)
                                                                                           ttATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGat                                                     <  1:3204209/148‑1 (MQ=255)
                                                                                            tATCCGTTTCCGCTTTGTCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGata                                                    <  1:1745392/148‑1 (MQ=255)
                                                                                            tATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGata                                                    <  1:2070257/148‑1 (MQ=255)
                                                                                             aTCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGatat                                                   <  2:1779886/148‑1 (MQ=255)
                                                                                             aTCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGatat                                                   <  1:1007588/148‑1 (MQ=255)
                                                                                             aTCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGatat                                                   <  2:264709/148‑1 (MQ=255)
                                                                                               ccGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATAttt                                                 >  1:2267613/1‑148 (MQ=255)
                                                                                               ccGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATAttt                                                 >  1:1025787/1‑148 (MQ=255)
                                                                                                 gTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATAttttt                                               >  1:187830/1‑148 (MQ=255)
                                                                                                   ttCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTTGCGAGATAATTCTACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGt                                             <  2:2861594/148‑1 (MQ=255)
                                                                                                       gCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATc                                         <  2:2607887/148‑1 (MQ=255)
                                                                                                        cTTTGCCCTTCACCACATCACTTTTTGTCGCTTTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATcc                                        <  1:2033949/148‑1 (MQ=255)
                                                                                                        cTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATcc                                        <  2:1996376/148‑1 (MQ=255)
                                                                                                         ttggCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCa                                       <  2:2981164/145‑1 (MQ=255)
                                                                                                          ttGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCAt                                      <  1:1495012/148‑1 (MQ=255)
                                                                                                           tGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATc                                     <  1:900799/148‑1 (MQ=255)
                                                                                                            gCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTatcaatca                                    <  2:2559294/148‑1 (MQ=255)
                                                                                                               cTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATa                                 >  2:1658749/1‑148 (MQ=255)
                                                                                                                ttCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATaa                                >  2:2247353/1‑148 (MQ=255)
                                                                                                                   accacATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACAt                             <  1:2417681/148‑1 (MQ=255)
                                                                                                                    ccacATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATc                            <  2:1369422/148‑1 (MQ=255)
                                                                                                                    ccacATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATc                            >  2:2570846/1‑148 (MQ=255)
                                                                                                                     cacaTCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCt                           >  1:2246995/1‑148 (MQ=255)
                                                                                                                      acaTCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTg                          >  1:368902/1‑148 (MQ=255)
                                                                                                                               tttGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTCTAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTa                 >  2:30820/1‑148 (MQ=255)
                                                                                                                                ttGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTaa                <  2:1810310/148‑1 (MQ=255)
                                                                                                                                   tCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGc             <  2:2835281/148‑1 (MQ=255)
                                                                                                                                     gCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGTCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCAt           <  2:2380012/148‑1 (MQ=255)
                                                                                                                                     gCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCAt           >  2:713753/1‑148 (MQ=255)
                                                                                                                                      cTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATa          >  2:133221/1‑148 (MQ=255)
                                                                                                                                        gTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATACg        >  1:2498621/1‑148 (MQ=255)
                                                                                                                                         tttGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATACGa       <  1:562215/148‑1 (MQ=255)
                                                                                                                                         tttGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGAGATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATACGa       >  2:2879805/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
GACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTC  >  CP009273/581490‑581779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑