Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 641,826 T→G 5.2% N71H (AAT→CAT)  citT ← citrate:succinate antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273641,8260TG 5.2% 562.2 / 21.2 192N71H (AAT→CAT) citTcitrate:succinate antiporter
Reads supporting (aligned to +/- strand):  ref base T (114/67);  new base G (6/4);  total (120/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

Only 100 of 199 total aligned reads displayed.

AAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGGGACAGGGATAAGAAA  >  CP009273/641679‑641973
                                                                                                                                                   |                                                                                                                                                   
aaaCTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAAtt                                                                                                                                                     >  1:1663152/1‑148 (MQ=255)
   cTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACt                                                                                                                                                  >  2:2415007/1‑148 (MQ=255)
    ttCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTAcgg                                                                                                                                                 <  1:3282418/148‑3 (MQ=255)
     tCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGc                                                                                                                                                <  1:542336/148‑1 (MQ=255)
       aTACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCa                                                                                                                                              >  2:1733327/1‑148 (MQ=255)
           ccTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATaa                                                                                                                                          >  1:1138374/1‑148 (MQ=255)
                 gCAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCaaa                                                                                                                                    >  2:189885/1‑148 (MQ=255)
                 gCAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCaaa                                                                                                                                    >  1:3045290/1‑148 (MQ=255)
                  cAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTTTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAAt                                                                                                                                   <  2:2046630/148‑1 (MQ=255)
                  cAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAAt                                                                                                                                   <  2:382577/148‑1 (MQ=255)
                   aaaaaTAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATa                                                                                                                                  <  2:699961/148‑1 (MQ=255)
                     aaataaatGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGt                                                                                                                                >  2:1401059/1‑148 (MQ=255)
                     aaataaatGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATGACTGCCAATAACGCAAATAGt                                                                                                                                >  1:2632239/1‑148 (MQ=255)
                      aataaatGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTATGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGACAATAAAGCAAATAGTa                                                                                                                               >  1:119920/1‑148 (MQ=255)
                       ataaatGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTaa                                                                                                                              >  1:38740/1‑148 (MQ=255)
                        taaatGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGAAAATAGTAAc                                                                                                                             >  2:92514/1‑148 (MQ=255)
                         aaatGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAAcc                                                                                                                            >  2:2512541/1‑148 (MQ=255)
                           atGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCACTAACGCACATAGTAACCGc                                                                                                                          >  2:1691072/1‑148 (MQ=255)
                           atGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCACATAGTAACCGc                                                                                                                          >  1:673326/1‑148 (MQ=255)
                                 cAAATACCATCCATACCGTTGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATaaa                                                                                                                    <  2:2228949/148‑1 (MQ=255)
                                  aaaTACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATaaaa                                                                                                                   <  1:1481843/148‑1 (MQ=255)
                                  aaaTACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATaaaa                                                                                                                   <  1:3124025/148‑1 (MQ=255)
                                      aCCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGa                                                                                                               <  2:966927/148‑1 (MQ=255)
                                       ccAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGAt                                                                                                              <  1:2008777/148‑1 (MQ=255)
                                         aGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCg                                                                                                            >  2:2818056/1‑148 (MQ=255)
                                            cATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATGACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTg                                                                                                         <  2:639547/148‑1 (MQ=255)
                                              tACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGtt                                                                                                       >  2:2671460/1‑148 (MQ=255)
                                               aCCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTg                                                                                                      >  1:1539051/1‑148 (MQ=255)
                                               aCCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTg                                                                                                      >  2:537141/1‑148 (MQ=255)
                                                 cGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGcc                                                                                                    >  1:769469/1‑148 (MQ=255)
                                                    ggtgCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGa                                                                                                 <  2:2986633/148‑1 (MQ=255)
                                                      tgCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACTCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGACAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAAt                                                                                               >  1:3006015/1‑148 (MQ=255)
                                                       gCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAAtt                                                                                              <  1:280622/148‑1 (MQ=255)
                                                           gAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCt                                                                                          >  2:202946/1‑148 (MQ=255)
                                                             aaaaCCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGTGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCg                                                                                        >  1:1734205/1‑148 (MQ=255)
                                                             aaaaCCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCg                                                                                        <  1:329436/148‑1 (MQ=255)
                                                             aaaaCCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCg                                                                                        <  1:2322164/148‑1 (MQ=255)
                                                              aaaCCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGa                                                                                       <  2:1168099/148‑1 (MQ=255)
                                                                aCCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAgg                                                                                     >  1:166677/1‑148 (MQ=255)
                                                                  cagccagGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGAt                                                                                   <  1:244870/148‑1 (MQ=255)
                                                                    gcccgGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCCTCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCa                                                                                 >  1:918817/1‑148 (MQ=255)
                                                                     ccagGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCAt                                                                                >  2:3032367/1‑148 (MQ=255)
                                                                     ccagGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCAt                                                                                >  2:852693/1‑148 (MQ=255)
                                                                      cagGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATg                                                                               <  1:566627/148‑1 (MQ=255)
                                                                        ggCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGcc                                                                             >  1:207515/1‑148 (MQ=255)
                                                                        ggCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGcc                                                                             >  2:2778894/1‑148 (MQ=255)
                                                                          ccccATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGa                                                                           >  1:68652/1‑148 (MQ=255)
                                                                           cccATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAGCCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGAc                                                                          >  1:1438300/1‑148 (MQ=255)
                                                                            ccATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACa                                                                         <  1:183093/148‑1 (MQ=255)
                                                                              aTTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAAt                                                                       >  1:345194/1‑148 (MQ=255)
                                                                              aTTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAAt                                                                       <  2:2104150/148‑1 (MQ=255)
                                                                               tttCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATc                                                                      >  1:649642/1‑148 (MQ=255)
                                                                               tttCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATc                                                                      >  2:2636875/1‑148 (MQ=255)
                                                                               tttCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATc                                                                      >  2:2637532/1‑148 (MQ=255)
                                                                               tttCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATc                                                                      >  2:2267840/1‑148 (MQ=255)
                                                                               tttCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATc                                                                      >  2:409517/1‑148 (MQ=255)
                                                                                ttCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCa                                                                     >  1:1801112/1‑148 (MQ=255)
                                                                                    gcgcCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGc                                                                 >  2:1585787/1‑148 (MQ=255)
                                                                                         tGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACaa                                                            >  2:684775/1‑148 (MQ=255)
                                                                                          gTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACaaa                                                           <  2:643827/148‑1 (MQ=255)
                                                                                            ttttGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAaca                                                         <  1:189885/148‑1 (MQ=255)
                                                                                              ttGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAAcaca                                                       <  2:769469/148‑1 (MQ=255)
                                                                                               tGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAg                                                      <  2:1647838/148‑1 (MQ=255)
                                                                                                   cATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGaa                                                  <  1:1835345/148‑1 (MQ=255)
                                                                                                      tAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTa                                               >  2:749474/1‑148 (MQ=255)
                                                                                                      tAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTa                                               >  2:3288578/1‑148 (MQ=255)
                                                                                                       aaaCTCTGGGTCATCTAATTCTTTGTCATCAAAGAGCAGTTAATTACTGCCTATAACGCAAATACTAAAAGCAATAACACTGATCTATGTTTACGGAATTGTATCGAGGATCATTCCGACAATCATTGCCACAAACACATCGAAGTaa                                              <  2:2589800/148‑1 (MQ=255)
                                                                                                        aaCGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAat                                             <  2:627715/148‑1 (MQ=255)
                                                                                                        aaCGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAat                                             >  1:1118918/1‑148 (MQ=255)
                                                                                                         aCGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAatg                                            <  1:3043674/148‑1 (MQ=255)
                                                                                                         aCGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAatg                                            <  1:2916330/148‑1 (MQ=255)
                                                                                                         aCGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAatg                                            <  1:126496/148‑1 (MQ=255)
                                                                                                         aCGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATGACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAatg                                            <  1:1043829/148‑1 (MQ=255)
                                                                                                               ggTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAatgccatgc                                      <  1:1006157/148‑1 (MQ=255)
                                                                                                               ggTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAatgccatgc                                      >  1:1400409/1‑148 (MQ=255)
                                                                                                                    gCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTgcg                                 >  1:1976491/1‑148 (MQ=255)
                                                                                                                      tAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACACTCATTGCCACAAACACAGCGAAGTAATGCCATGCCTgcggc                               >  2:3205945/1‑148 (MQ=255)
                                                                                                                      tAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTgcggc                               >  1:2543969/1‑148 (MQ=255)
                                                                                                                      tAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTgcggc                               >  1:1053643/1‑148 (MQ=255)
                                                                                                                       aaTTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTgcggcg                              >  1:2109525/1‑148 (MQ=255)
                                                                                                                        aTTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGcggcgg                             >  1:2482623/1‑148 (MQ=255)
                                                                                                                         ttCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCggcggc                            >  2:3108802/1‑148 (MQ=255)
                                                                                                                          tCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCa                           >  1:3195980/1‑148 (MQ=255)
                                                                                                                                   tCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCgg                  >  1:46211/1‑148 (MQ=255)
                                                                                                                                   tCAAAGAGCAGGTAATGACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCgg                  >  2:185353/1‑148 (MQ=255)
                                                                                                                                       agagCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGGGAc              >  2:1969038/1‑148 (MQ=255)
                                                                                                                                       agagCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGGGAc              >  1:2243493/1‑148 (MQ=255)
                                                                                                                                       agagCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGGGAc              >  1:2417785/1‑148 (MQ=255)
                                                                                                                                       agagCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGGGAc              >  1:2131305/1‑148 (MQ=255)
                                                                                                                                         agCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGGGACAg            <  1:2415007/148‑1 (MQ=255)
                                                                                                                                           cAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCTTGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGGGACAggg          >  2:1958721/1‑148 (MQ=255)
                                                                                                                                           cAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGGGACAggg          <  2:1663152/148‑1 (MQ=255)
                                                                                                                                              gTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGGGACAGGGATa       >  2:598558/1‑148 (MQ=255)
                                                                                                                                               tAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGGGACAGGGATaa      >  1:1285432/1‑148 (MQ=255)
                                                                                                                                                aaTTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGGGACAGGGATAAg     >  2:434963/1‑148 (MQ=255)
                                                                                                                                                 aTTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGGGACAGGGATAAGa    >  1:572841/1‑148 (MQ=255)
                                                                                                                                                 aTTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGGGACAGGGATAAGa    >  1:1610029/1‑148 (MQ=255)
                                                                                                                                                  ttACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGGGACAGGGATAAGaa   >  2:1017773/1‑148 (MQ=255)
                                                                                                                                                   tACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGGGACAGGGATAAGaaa  >  1:1284630/1‑148 (MQ=255)
                                                                                                                                                   tACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGGGACAGGGATAAGaaa  >  1:1285259/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGGGACAGGGATAAGAAA  >  CP009273/641679‑641973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑