Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 80,123 C→T 100% G25S (GGC→AGC)  leuL ← leu operon leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927380,1230CT99.5% 665.7 / ‑4.4 200G25S (GGC→AGC) leuLleu operon leader peptide
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (102/97);  total (102/98)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 200 total aligned reads displayed.

CAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACG  >  CP009273/79981‑80266
                                                                                                                                              |                                                                                                                                               
cAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCa                                                                                                                                            >  1:271590/1‑148 (MQ=255)
  aTGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACt                                                                                                                                          >  1:2962844/1‑148 (MQ=255)
   tgtgGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTc                                                                                                                                         >  1:3091861/1‑148 (MQ=255)
   tgtgGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTc                                                                                                                                         >  2:1342174/1‑148 (MQ=255)
   tgtgGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTc                                                                                                                                         >  2:2560077/1‑148 (MQ=255)
     tgGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGt                                                                                                                                       <  1:1326603/148‑1 (MQ=255)
     tgGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGt                                                                                                                                       <  2:1339082/148‑1 (MQ=255)
      ggTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTc                                                                                                                                      >  2:2188702/1‑148 (MQ=255)
       gTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCt                                                                                                                                     >  1:2931456/1‑148 (MQ=255)
       gTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCt                                                                                                                                     >  1:2008536/1‑148 (MQ=255)
       gTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCt                                                                                                                                     <  2:540110/148‑1 (MQ=255)
        tATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTa                                                                                                                                    >  1:993087/1‑148 (MQ=255)
        tATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTa                                                                                                                                    >  1:1187215/1‑148 (MQ=255)
          tCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTAcc                                                                                                                                  >  2:597306/1‑148 (MQ=255)
          tCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTAcc                                                                                                                                  >  1:198131/1‑148 (MQ=255)
           cGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACcg                                                                                                                                 >  1:150064/1‑148 (MQ=255)
           cGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACcg                                                                                                                                 >  1:2821224/1‑148 (MQ=255)
              aaaTAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCgcgc                                                                                                                              >  2:875191/1‑148 (MQ=255)
               aataatGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCa                                                                                                                             >  1:2079248/1‑148 (MQ=255)
                ataatGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCaa                                                                                                                            <  2:3196768/148‑1 (MQ=255)
                 taatGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCaaa                                                                                                                           >  2:254877/1‑148 (MQ=255)
                  aatGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAAGGGCGCGGGTATTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAaag                                                                                                                          <  2:685840/148‑1 (MQ=255)
                  aatGACGTGCGGGATCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAaag                                                                                                                          <  2:2283027/148‑1 (MQ=255)
                   atGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAaaga                                                                                                                         >  2:1431990/1‑148 (MQ=255)
                    tGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAaagaa                                                                                                                        >  1:2466000/1‑148 (MQ=255)
                         tGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGc                                                                                                                   >  2:2697290/1‑148 (MQ=255)
                           cTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGt                                                                                                                 <  1:1517070/148‑1 (MQ=255)
                           cTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGt                                                                                                                 <  1:746226/148‑1 (MQ=255)
                            tGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGtt                                                                                                                <  1:1342276/148‑1 (MQ=255)
                            tGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGtt                                                                                                                <  1:2989117/148‑1 (MQ=255)
                            tGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGtt                                                                                                                >  1:3102204/1‑148 (MQ=255)
                            tGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGtt                                                                                                                <  1:462438/148‑1 (MQ=255)
                             ggCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGttt                                                                                                               >  1:900093/1‑148 (MQ=255)
                                   tGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAATAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCCAAGAAGATGCGTTtagtagt                                                                                                         >  2:2156294/1‑148 (MQ=255)
                                      tttGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtagt                                                                                                      >  1:2304039/1‑148 (MQ=255)
                                      tttGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtagt                                                                                                      >  2:1770816/1‑148 (MQ=255)
                                        tGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtagtag                                                                                                    <  2:76135/148‑1 (MQ=255)
                                         gggTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGa                                                                                                   <  1:1214272/148‑1 (MQ=255)
                                         gggTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGa                                                                                                   <  1:597306/148‑1 (MQ=255)
                                            tCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCg                                                                                                <  2:2550829/148‑1 (MQ=255)
                                            tCCTTGTCTCGTTTAGAGCGCCTCGCTTCGGGCATACAACAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACGCGTCTACCGCGCAAAGACGATGCGTTTAGTAGTAGTAGACCg                                                                                                <  2:3166751/148‑1 (MQ=255)
                                             ccTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGa                                                                                               >  1:2089716/1‑148 (MQ=255)
                                                tGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATaa                                                                                            >  2:193623/1‑148 (MQ=255)
                                                tGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATaa                                                                                            >  2:1396623/1‑148 (MQ=255)
                                                 gTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATaaa                                                                                           <  2:3089961/148‑1 (MQ=255)
                                                     ctTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGa                                                                                       >  1:1874584/1‑148 (MQ=255)
                                                           gagCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGTCTTCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGAtg                                                                                 >  1:1446687/1‑148 (MQ=255)
                                                             gcgcCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGAtgtg                                                                               <  1:511876/148‑1 (MQ=255)
                                                                 cTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTc                                                                           <  2:2764552/148‑1 (MQ=255)
                                                                 cTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTc                                                                           >  2:2849402/1‑148 (MQ=255)
                                                                  tCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCa                                                                          <  2:2129501/148‑1 (MQ=255)
                                                                     cTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTTACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTa                                                                       >  1:2429239/1‑148 (MQ=255)
                                                                        cGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTCGTAGACCGATAAAGCGAACGATGTGAGTCATTAAAt                                                                    >  1:1599896/1‑148 (MQ=255)
                                                                        cGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAAt                                                                    <  1:1002449/148‑1 (MQ=255)
                                                                              tataaaaaCCCGCGCAATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGAGCAAAGAAGATGCGTTTAGTATTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTc                                                              <  2:3062583/145‑1 (MQ=255)
                                                                               aaaaaaaCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTcc                                                             <  1:786774/148‑1 (MQ=255)
                                                                                  aaaaCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCCTTAAATCAGCTCCAGa                                                          >  2:1135705/1‑148 (MQ=255)
                                                                                  aaaaCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGa                                                          <  1:2222389/148‑1 (MQ=255)
                                                                                  aaaaCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGa                                                          <  1:2665581/148‑1 (MQ=255)
                                                                                  aaaaCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGa                                                          >  2:2226481/1‑148 (MQ=255)
                                                                                  aaaaCCCGCGCAATGGCGCGGGTATTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGa                                                          <  1:3161559/148‑1 (MQ=255)
                                                                                      cccGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGa                                                                              >  1:1788716/1‑124 (MQ=255)
                                                                                         gcgcACTGGCGCGGGTTTTTTGTTTGACTGCGTTCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCTCGCAATGAAGATGCGTTTAGTAGTAGTATACCGATAAAGCTAACGATTTGATTCATTAAATCAGCTCCAGATTAATGc                                                   <  2:2664076/148‑1 (MQ=255)
                                                                                         gcgcAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGc                                                   >  2:341584/1‑148 (MQ=255)
                                                                                           gcAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGa                                                 <  2:1594576/148‑1 (MQ=255)
                                                                                           gcAATGGCGCGGGTTTTTTGTTGGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGa                                                 <  2:431404/148‑1 (MQ=255)
                                                                                             aaTGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGata                                               <  1:3245159/148‑1 (MQ=255)
                                                                                               tGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATg                                             >  2:1952812/1‑148 (MQ=255)
                                                                                               tGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATg                                             >  1:2345439/1‑148 (MQ=255)
                                                                                                  cgcgGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCtt                                          <  1:655478/148‑1 (MQ=255)
                                                                                                       gTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTaga                                     >  2:1143189/1‑148 (MQ=255)
                                                                                                       gTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTaga                                     <  2:283578/148‑1 (MQ=255)
                                                                                                       gTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTaga                                     >  2:2933749/1‑148 (MQ=255)
                                                                                                         tttttGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGt                                   >  1:1227716/1‑148 (MQ=255)
                                                                                                           tttgtttgACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTa                                 >  1:2010669/1‑148 (MQ=255)
                                                                                                            ttgtttgACTGCGTGCTGGCTTAATGCTGTATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTAc                                >  2:94785/1‑148 (MQ=255)
                                                                                                            ttgtttgACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTAc                                <  1:2791130/148‑1 (MQ=255)
                                                                                                             tgtttgACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACt                               <  1:1431990/148‑1 (MQ=255)
                                                                                                             tgtttgACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACt                               <  2:2962892/148‑1 (MQ=255)
                                                                                                               tttgACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTgg                             >  1:1580101/1‑148 (MQ=255)
                                                                                                                ttgACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGa                            <  1:1683597/148‑1 (MQ=255)
                                                                                                                   aCTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATAc                         >  2:2912166/1‑148 (MQ=255)
                                                                                                                     tGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACaa                       >  2:626181/1‑148 (MQ=255)
                                                                                                                       cGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACaaaa                     <  2:3154951/148‑1 (MQ=255)
                                                                                                                       cGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACaaaa                     <  1:3228716/148‑1 (MQ=255)
                                                                                                                       cGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACaaaa                     <  2:2331135/148‑1 (MQ=255)
                                                                                                                        gTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACaaaaa                    <  1:915941/148‑1 (MQ=255)
                                                                                                                        gTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACaaaaa                    <  2:1268424/148‑1 (MQ=255)
                                                                                                                            tGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGa                <  2:3102183/148‑1 (MQ=255)
                                                                                                                            tGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGa                <  2:1191033/148‑1 (MQ=255)
                                                                                                                            tGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGa                <  2:1312909/148‑1 (MQ=255)
                                                                                                                               cTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGt             <  2:502954/148‑1 (MQ=255)
                                                                                                                               cTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGt             <  2:895036/148‑1 (MQ=255)
                                                                                                                                ttAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACTGATGTc            >  2:2403660/1‑148 (MQ=255)
                                                                                                                                ttAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTc            <  1:2597238/148‑1 (MQ=255)
                                                                                                                                 tAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCa           <  2:3031562/148‑1 (MQ=255)
                                                                                                                                 tAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCa           <  2:1384811/148‑1 (MQ=255)
                                                                                                                                     gCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAAccc       >  1:807508/1‑148 (MQ=255)
                                                                                                                                        ggATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGa    >  1:62955/1‑148 (MQ=255)
                                                                                                                                          aTGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACg  >  2:1799212/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                               
CAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACG  >  CP009273/79981‑80266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑