Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 666,998 G→T 11.3% L22I (CTT→ATT)  holA ← DNA polymerase III, delta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273666,9980GT11.3% 543.0 / 32.7 169L22I (CTT→ATT) holADNA polymerase III, delta subunit
Reads supporting (aligned to +/- strand):  ref base G (87/62);  new base T (11/9);  total (98/71)
Fisher's exact test for biased strand distribution p-value = 8.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 169 total aligned reads displayed.

GCCTGGCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAAT  >  CP009273/666853‑667144
                                                                                                                                                 |                                                                                                                                                  
gCCTGGCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGAt                                                                                                                                                  >  2:740918/1‑148 (MQ=255)
 ccTGGCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATa                                                                                                                                                 >  1:2723329/1‑148 (MQ=255)
   tGGCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATAcg                                                                                                                                               <  2:2854010/148‑1 (MQ=255)
   tGGCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATAcg                                                                                                                                               <  2:1203337/148‑1 (MQ=255)
   tGGCATAACGAAAAGATCGCATTCCAGTCAGGGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGCCTTTCCTGCAATAACAGAGGATCGATACCAAGTAAAAGATAct                                                                                                                                               >  2:2585711/1‑147 (MQ=255)
     gCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATAcgcc                                                                                                                                             >  1:1617178/1‑148 (MQ=255)
      cATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATAcgccg                                                                                                                                            <  2:2968194/148‑1 (MQ=255)
       aTAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGGTACCAAGTAAAAGATAcgccgc                                                                                                                                           >  2:2691677/1‑148 (MQ=255)
       aTAAAGAAAATAAAGCATTCAAGTAAGTGATGGGAACAATGGAAAAAGTGTTGTGTTCTTCGAATCCTTGTGACTCAGATACCTGACGAACAGCGTCCTGGCTTTCCTGAAATAACATACGATCGTTACAAAGTAAAAGATActccgc                                                                                                                                           >  2:2693452/1‑148 (MQ=255)
         aaCGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCcgcgc                                                                                                                                         >  1:69737/1‑148 (MQ=255)
             aaaaGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAg                                                                                                                                     >  2:1575638/1‑148 (MQ=255)
             aaaaGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAg                                                                                                                                     >  1:2216759/1‑148 (MQ=255)
               aaGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGcc                                                                                                                                   >  1:940592/1‑148 (MQ=255)
                aGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGccc                                                                                                                                  <  2:659855/148‑1 (MQ=255)
                aGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGccc                                                                                                                                  >  1:2477352/1‑148 (MQ=255)
                aGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGccc                                                                                                                                  >  1:623018/1‑148 (MQ=255)
                  aTCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCtt                                                                                                                                >  1:1753623/1‑148 (MQ=255)
                     gCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCAt                                                                                                                             <  2:1089166/148‑1 (MQ=255)
                     gCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCAt                                                                                                                             <  2:2267454/148‑1 (MQ=255)
                      cATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCAtt                                                                                                                            <  1:3252192/148‑1 (MQ=255)
                         tCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAg                                                                                                                         >  2:527859/1‑148 (MQ=255)
                          ccAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGc                                                                                                                        <  1:770513/148‑1 (MQ=255)
                            agtcagtGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCtt                                                                                                                      >  2:1087082/1‑147 (MQ=255)
                             gtcagtGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTgc                                                                                                                     >  2:2114292/1‑148 (MQ=255)
                             gtcagtGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTgc                                                                                                                     >  2:964887/1‑148 (MQ=255)
                                   gtTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCgg                                                                                                               >  2:680172/1‑148 (MQ=255)
                                     tGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAg                                                                                                             <  2:1417665/148‑1 (MQ=255)
                                     tGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAg                                                                                                             <  2:503977/148‑1 (MQ=255)
                                     tGGGATCAATGGAAAAAGTGTGGTGTTCTTAGAATACTTGTGCCGCAGCTACCTGACTAACAGCGTCCTGGCTTTCCTGCAATAACATAGGATGGTTACCAAGTAAAAGATACGCCGCGAGCAGCCCTTCATTGAGCTGCGCGCGGAg                                                                                                             <  1:754751/148‑1 (MQ=255)
                                       ggATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAgtt                                                                                                           >  1:832298/1‑148 (MQ=255)
                                         aTCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAgttgt                                                                                                         >  2:1722487/1‑148 (MQ=255)
                                          tCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAgttgtt                                                                                                        >  2:2003119/1‑148 (MQ=255)
                                          tCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAgttgtt                                                                                                        >  2:2003107/1‑148 (MQ=255)
                                           cAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTc                                                                                                       >  2:1940186/1‑148 (MQ=255)
                                           cAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTc                                                                                                       <  1:1749808/148‑1 (MQ=255)
                                             aTGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCg                                                                                                     <  1:1555648/148‑1 (MQ=255)
                                             aTGGAAAAAGTGTGGTGGTCTTCGAATCCTTGTGCCGCAGCGACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCg                                                                                                     <  1:1555678/148‑1 (MQ=255)
                                              tGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCgg                                                                                                    >  2:3135811/1‑148 (MQ=255)
                                              tGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCgg                                                                                                    >  2:3246828/1‑148 (MQ=255)
                                              tGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCgg                                                                                                    >  2:1788423/1‑148 (MQ=255)
                                               ggAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGGGCAGCCCGTCAATGAGCTGCGCGCGGAGTTGTTCCggg                                                                                                   >  1:2796431/1‑148 (MQ=255)
                                               ggAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCggg                                                                                                   >  2:88323/1‑148 (MQ=255)
                                                gAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCCGCCCTTCATTGAGCTGCGCGCGGAGTTGGTGCGGGt                                                                                                  >  2:2236880/1‑148 (MQ=255)
                                                gAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTTAGCTGCGCGCGGAGTTGTTCCGGGt                                                                                                  >  1:1613892/1‑148 (MQ=255)
                                                 aaaaaGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTa                                                                                                 >  1:2663622/1‑148 (MQ=255)
                                                  aaaaGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGTCATTGATCTGCGCGCGGAGTTGTTGCGGGTAc                                                                                                >  1:2505062/1‑148 (MQ=255)
                                                  aaaaGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGTCATTGAGCTGCGCGCGGGGTTGTTCCGGGTAc                                                                                                >  1:2505085/1‑148 (MQ=255)
                                                    aaGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACaa                                                                                              <  1:1978878/148‑1 (MQ=255)
                                                       tgtgGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCg                                                                                           >  2:2768106/1‑148 (MQ=255)
                                                        gtggtgTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGa                                                                                          <  2:2046193/148‑1 (MQ=255)
                                                         tggtgTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGaa                                                                                         <  2:2962651/148‑1 (MQ=255)
                                                           gtgtTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCAATGAGCGGCGCGCGGAGTTGTTCCGGGTAAAACCGAatc                                                                                       >  1:2966751/1‑148 (MQ=255)
                                                             gtTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAatcat                                                                                     <  1:3271382/148‑1 (MQ=255)
                                                             gtTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAatcat                                                                                     <  1:1729752/148‑1 (MQ=255)
                                                                cttcGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAg                                                                                  <  2:1449565/148‑1 (MQ=255)
                                                                     aaTCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTCCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTAcc                                                                             <  2:983770/148‑1 (MQ=255)
                                                                     aaTCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTAcc                                                                             >  2:2200656/1‑148 (MQ=255)
                                                                      aTCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTTAGGTGCGCGCGGAGTTGTTACGGGTACAACCCAATAAACAGTTAccc                                                                            >  1:1823949/1‑148 (MQ=255)
                                                                      aTCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTAccc                                                                            >  2:1249682/1‑148 (MQ=255)
                                                                        ccTTTTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGTCATTTTGTTGCGCGCGGAGTTTTTCCCGGTCCAACCGAATCATCAGTTAACCAg                                                                          >  1:1029483/1‑148 (MQ=255)
                                                                        ccTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAg                                                                          >  1:1029516/1‑148 (MQ=255)
                                                                           tgtgCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGTCATTGAGCTGCGCGCGGAGTTTTTCCGGGTACAACCGAATCATCAGTTACCCAGCgg                                                                       >  1:161149/1‑147 (MQ=255)
                                                                             tgCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCgggg                                                                     >  1:2384896/1‑148 (MQ=255)
                                                                              gCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGAGACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCgtggt                                                                    <  1:1151886/148‑1 (MQ=255)
                                                                                    gCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCATCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGAc                                                              >  1:459563/1‑148 (MQ=255)
                                                                                     cTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCCGCCCGGCAATGTGCGGCGCGCGGAGTTGGTGCGGGGACAACCGACTCATCAGTTACCCAGCGGGGTGGCGAcg                                                             >  1:363759/1‑148 (MQ=255)
                                                                                     cTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGACTCAGCAGTTACCCAGCGTGGTGGAGAcg                                                             >  2:3078631/1‑148 (MQ=255)
                                                                                      tACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGAcgc                                                            <  2:3184920/148‑1 (MQ=255)
                                                                                        ccTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGt                                                          >  2:1527280/1‑148 (MQ=255)
                                                                                        ccTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGTCATTGTGCTGCGCGCGGTGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGt                                                          >  1:1210160/1‑148 (MQ=255)
                                                                                          tGACGACCAGCGTACTGGCTTTCCTGCAACAACAGAGTATCGTTACCAAGTAAAAGAGACGCCGCGCGCCGCCCTTCCTTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGc                                                        <  1:123442/148‑1 (MQ=255)
                                                                                             cGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAgg                                                     <  1:133421/148‑1 (MQ=255)
                                                                                                aCAGCGTCCTGGCTGTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGt                                                  <  1:3054686/148‑1 (MQ=255)
                                                                                                  aGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTg                                                <  2:497188/148‑1 (MQ=255)
                                                                                                  aGCGTCCTGGCTTTCCTGCAATAACAGAGGACCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTg                                                <  2:366401/148‑1 (MQ=255)
                                                                                                    cGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCCGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGGGGTGGAGACGCGTGCAGGCGTTGcc                                              >  1:2529091/1‑148 (MQ=255)
                                                                                                      tCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCgg                                            >  2:1322774/1‑148 (MQ=255)
                                                                                                      tCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGGGGTGGAGACGCGTGCAGGCGTTGCCgg                                            >  2:2803721/1‑148 (MQ=255)
                                                                                                      tCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGGGGTGGAGACGCGTGCAGGCGTTGCCgg                                            >  2:2970649/1‑148 (MQ=255)
                                                                                                         tGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGt                                         <  2:1651998/148‑1 (MQ=255)
                                                                                                           gCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTGATTGAGCTGCGCGCGGCGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTAt                                       >  1:2696447/1‑148 (MQ=255)
                                                                                                                    cAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTgg                              <  1:2159189/148‑1 (MQ=255)
                                                                                                                          cAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACg                        <  1:965048/148‑1 (MQ=255)
                                                                                                                           agagGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGt                       <  1:828298/148‑1 (MQ=255)
                                                                                                                             agGATCGTTACCAAGTAAAAGATACGCCGCGCACAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCt                     >  1:2871740/1‑148 (MQ=255)
                                                                                                                                aTCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGtt                  <  2:319447/148‑1 (MQ=255)
                                                                                                                                aTCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGtt                  <  2:2216759/148‑1 (MQ=255)
                                                                                                                                aTCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGGGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGtt                  >  2:1991927/1‑148 (MQ=255)
                                                                                                                                   gTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGttctt               <  1:2003107/148‑1 (MQ=255)
                                                                                                                                   gTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGttctt               <  1:2003119/148‑1 (MQ=255)
                                                                                                                                    ttACCAAGTAAAATATACGCCGCGCGCACCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCCGTTACCCAGCGGGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGttcttc              >  1:2958224/1‑148 (MQ=255)
                                                                                                                                     tACCAAGTAAAAGATACGCCGCGCGCAGCCCGTCAGTTAGCTGCGCGCGGAGTTGTTCCGGGTACAACCCAATAAAAAGTTACCCAGCGGGGTGGAGACGCGTGCAGGCGTTGCCGGCGTATCCGTTGTGGGCGACGGCTGttcttag             >  1:2634356/1‑146 (MQ=255)
                                                                                                                                     tACCAAGTAAAAGATACGCCGCGCGCAGCCCATCATTGAGCTGCGCGCGGAGTTGTTGCGGGTACAACCCAAACATCAGTTACCCCGCGGGGTGGTGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGGCTGttattgg             >  1:2634358/1‑146 (MQ=255)
                                                                                                                                        cAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTtcgtcg          >  2:2332436/1‑148 (MQ=255)
                                                                                                                                          aGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGa        <  2:1373230/148‑1 (MQ=255)
                                                                                                                                           gTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGaa       <  1:88323/148‑1 (MQ=255)
                                                                                                                                              aaaGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATGAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGGCGGCTGTTCTTAGTCGGAACGa    >  2:306036/1‑148 (MQ=255)
                                                                                                                                               aaGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACCACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGaa   >  2:988317/1‑148 (MQ=255)
                                                                                                                                               aaGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGaa   <  2:2723329/148‑1 (MQ=255)
                                                                                                                                                aGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAat  <  2:1665884/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GCCTGGCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAAT  >  CP009273/666853‑667144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑