Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 705,588 C→A 100% intergenic (+16/+68) chiQ → / ← fur chitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273705,5880CA99.7% 692.9 / ‑6.0 217intergenic (+16/+68)chiQ/furchitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (115/101);  total (116/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 217 total aligned reads displayed.

GCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGT  >  CP009273/705444‑705732
                                                                                                                                                |                                                                                                                                                
gcagACGAGGAAAGTGTTCGCGTTCTTGATTATCAGGAGTGTCTGCGTGGAACTGAATCCGGATATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTGTGGTAGGAAATACGCAGTAATATCAAATAAGTTAGAGCTGTAACTATCg                                                                                                                                               <  2:2591460/145‑1 (MQ=255)
gCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCg                                                                                                                                               <  2:2320010/148‑1 (MQ=255)
  cGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCt                                                                                                                                             <  1:146247/148‑1 (MQ=255)
   gACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCtt                                                                                                                                            <  2:2105342/148‑1 (MQ=255)
    aCCAGGAAATTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGACTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCttt                                                                                                                                           <  1:2163140/148‑1 (MQ=255)
    aCCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACAGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCttt                                                                                                                                           >  2:1679512/1‑148 (MQ=255)
      cAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTc                                                                                                                                         <  1:2910731/148‑1 (MQ=255)
        ggAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCtt                                                                                                                                       >  1:2901906/1‑148 (MQ=255)
        ggAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCtt                                                                                                                                       >  2:1408384/1‑148 (MQ=255)
        ggAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCtt                                                                                                                                       >  2:1408327/1‑148 (MQ=255)
        ggAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCtt                                                                                                                                       <  1:2939050/148‑1 (MQ=255)
        ggAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCtt                                                                                                                                       <  1:782679/148‑1 (MQ=255)
          aaaGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTAt                                                                                                                                     <  2:2056545/148‑1 (MQ=255)
             gtgtACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTc                                                                                                                                  <  2:1929540/148‑1 (MQ=255)
              tgtACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTcc                                                                                                                                 <  2:754546/148‑1 (MQ=255)
              tgtACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTcc                                                                                                                                 <  1:2248340/148‑1 (MQ=255)
              tgtACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTcc                                                                                                                                 <  1:861764/148‑1 (MQ=255)
               gtACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTccc                                                                                                                                <  1:1621085/148‑1 (MQ=255)
               gtACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTccc                                                                                                                                <  2:196049/148‑1 (MQ=255)
                   gcgTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGc                                                                                                                            <  2:2957352/148‑1 (MQ=255)
                    cgTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCa                                                                                                                           >  1:1544312/1‑148 (MQ=255)
                       aCTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATaa                                                                                                                        >  2:2997749/1‑148 (MQ=255)
                       aCTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATaa                                                                                                                        <  2:222404/148‑1 (MQ=255)
                        cTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATaaa                                                                                                                       <  1:1619240/148‑1 (MQ=255)
                         tGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATaaaa                                                                                                                      <  2:1258295/148‑1 (MQ=255)
                         tGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATaaaa                                                                                                                      >  2:2507254/1‑148 (MQ=255)
                              tATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGcc                                                                                                                 >  1:1369998/1‑148 (MQ=255)
                                 cagcagTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAAc                                                                                                              <  1:279342/148‑1 (MQ=255)
                                   gcagTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGTCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAAccc                                                                                                            >  2:2861736/1‑148 (MQ=255)
                                    cagTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCg                                                                                                           >  1:1920271/1‑148 (MQ=255)
                                         tCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGt                                                                                                      <  1:2407824/148‑1 (MQ=255)
                                          cTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGtt                                                                                                     >  2:348054/1‑148 (MQ=255)
                                            gCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTgg                                                                                                   <  1:384439/148‑1 (MQ=255)
                                              gTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCt                                                                                                 <  2:495060/148‑1 (MQ=255)
                                               tGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCtt                                                                                                <  2:106731/148‑1 (MQ=255)
                                               tGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCtt                                                                                                <  2:305631/148‑1 (MQ=255)
                                                 cAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCtttt                                                                                              <  1:1243294/148‑1 (MQ=255)
                                                 cAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCtttt                                                                                              <  1:495504/148‑1 (MQ=255)
                                                   aCGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtct                                                                                            <  1:788581/148‑1 (MQ=255)
                                                   aCGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtct                                                                                            <  1:2296454/148‑1 (MQ=255)
                                                      cAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATCAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGt                                                                                         <  1:1270486/148‑1 (MQ=255)
                                                      cAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGt                                                                                         <  1:2919657/148‑1 (MQ=255)
                                                       aaaCCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGtt                                                                                        <  2:2761827/148‑1 (MQ=255)
                                                        aaCCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTc                                                                                       >  1:3053010/1‑148 (MQ=255)
                                                         aCCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTGATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCa                                                                                      <  2:1311699/148‑1 (MQ=255)
                                                         aCCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCa                                                                                      >  1:1124190/1‑148 (MQ=255)
                                                         aCCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCa                                                                                      >  1:2351496/1‑148 (MQ=255)
                                                          ccGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAg                                                                                     >  1:1430677/1‑148 (MQ=255)
                                                            ggAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggc                                                                                   <  2:1244938/148‑1 (MQ=255)
                                                              aaaTGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggctg                                                                                 >  2:358857/1‑148 (MQ=255)
                                                                 tGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggctggct                                                                              <  1:2023365/148‑1 (MQ=255)
                                                                  gATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCtt                                                                             >  2:2928386/1‑148 (MQ=255)
                                                                  gATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCtt                                                                             <  2:1194443/148‑1 (MQ=255)
                                                                   aTCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTa                                                                            >  1:965669/1‑148 (MQ=255)
                                                                     cAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTAtt                                                                          <  2:1983382/148‑1 (MQ=255)
                                                                         cGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGcc                                                                      <  1:1933098/148‑1 (MQ=255)
                                                                         cGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGcc                                                                      <  2:1740527/148‑1 (MQ=255)
                                                                           gTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCtt                                                                    >  1:3237654/1‑148 (MQ=255)
                                                                           gTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCtt                                                                    >  1:3140928/1‑148 (MQ=255)
                                                                              aaaGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGt                                                                 >  2:1573159/1‑148 (MQ=255)
                                                                                 gCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTGTCTTATTTCACTTTCATAAAAAAGCAAACCCGCAGGTTGGATTTTCTCGTTAAGGATGGCTTATTTGACTTCGTgcg                                                              >  1:137136/1‑148 (MQ=255)
                                                                                        gtgATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTc                                                       >  2:2791805/1‑148 (MQ=255)
                                                                                         tgATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCa                                                      <  1:3298067/148‑1 (MQ=255)
                                                                                             aaaGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCtt                                                  >  2:2729756/1‑148 (MQ=255)
                                                                                                gTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTcgc                                               >  1:1330366/1‑148 (MQ=255)
                                                                                                 tCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTcgcg                                              >  2:1052218/1‑148 (MQ=255)
                                                                                                   tGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGc                                            >  1:470121/1‑148 (MQ=255)
                                                                                                     gCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCaa                                          <  1:410169/148‑1 (MQ=255)
                                                                                                     gCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCaa                                          <  1:358857/148‑1 (MQ=255)
                                                                                                     gCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCaa                                          <  1:1020151/148‑1 (MQ=255)
                                                                                                        ggAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCg                                       >  1:288587/1‑148 (MQ=255)
                                                                                                         taaaTACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGc                                      >  1:2687720/2‑148 (MQ=255)
                                                                                                         gAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGc                                      >  1:2524187/1‑148 (MQ=255)
                                                                                                         gAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGc                                      >  1:2914055/1‑148 (MQ=255)
                                                                                                           aaTACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCt                                    >  1:1771938/1‑148 (MQ=255)
                                                                                                                gCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGc                               <  1:2971062/148‑1 (MQ=255)
                                                                                                                   gTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCTTAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGcaca                            <  2:336654/148‑1 (MQ=255)
                                                                                                                    taataaCAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAg                           <  2:2431749/148‑1 (MQ=255)
                                                                                                                      ataaCAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTg                         <  1:1282275/148‑1 (MQ=255)
                                                                                                                      ataaCAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTg                         >  1:1615532/1‑148 (MQ=255)
                                                                                                                       taaCAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAATCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGa                        >  2:1323357/1‑148 (MQ=255)
                                                                                                                       taaCAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGa                        >  1:734737/1‑148 (MQ=255)
                                                                                                                       taaCAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGa                        >  1:597630/1‑148 (MQ=255)
                                                                                                                        aaCAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGAc                       >  2:811534/1‑148 (MQ=255)
                                                                                                                         aCAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGAcc                      >  1:1302505/1‑148 (MQ=255)
                                                                                                                          cAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCg                     >  2:2258910/1‑148 (MQ=255)
                                                                                                                          cAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCg                     >  2:1892795/1‑148 (MQ=255)
                                                                                                                                aGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGa               <  1:1513954/148‑1 (MQ=255)
                                                                                                                                 gTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGAt              >  2:3224550/1‑148 (MQ=255)
                                                                                                                                 gTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGAt              >  2:372327/1‑148 (MQ=255)
                                                                                                                                  tGAGAGCTGTAACTCTCGCTTTTCTTATTTCCGTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTGCGGCCCAGTGACCGTAAAGATa             >  2:2121372/1‑148 (MQ=255)
                                                                                                                                  tGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGACAACCCGCAGGTTGGCTTGTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGAGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACAGTAAAtata             >  2:1267672/1‑148 (MQ=255)
                                                                                                                                     gagCTGTAACTATCGCTGTTCTTATTTCCCGTGCAGAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATagag          <  2:1773877/148‑1 (MQ=255)
                                                                                                                                      agCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAgaga         >  1:1909743/1‑148 (MQ=255)
                                                                                                                                         tGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACtg      >  1:1669749/1‑148 (MQ=255)
                                                                                                                                          gTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACtgt     >  2:2691408/1‑148 (MQ=255)
                                                                                                                                          gTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACtgt     >  2:2658842/1‑148 (MQ=255)
                                                                                                                                          gTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACtgt     >  2:411027/1‑148 (MQ=255)
                                                                                                                                            aaCTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTgg   >  1:1173799/1‑148 (MQ=255)
                                                                                                                                             aCTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGt  >  2:625558/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGT  >  CP009273/705444‑705732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑